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Protein

Inorganic triphosphatase

Gene

ygiF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the hydrolysis of the beta-gamma-phosphoanhydride linkage of triphosphate-containing substrates (inorganic or nucleoside-linked). Catalyzes the hydrolysis of inorganic triphosphate (PPPi), which could be cytotoxic because of its high affinity for calcium ion, thereby interfering with calcium signaling. It also hydrolyzes slowly thiamine triphosphate (ThTP). YgiF is a specific PPPase, but it contributes only marginally to the total PPPase activity in E.coli, where the main enzyme responsible for hydrolysis of PPPi is inorganic pyrophosphatase (PPase).1 Publication

Catalytic activityi

Triphosphate + H2O = diphosphate + phosphate.1 Publication

Enzyme regulationi

Inhibited by calcium ion and activated by magnesium ion.1 Publication

Kineticsi

  1. KM=270 µM for inorganic triphosphate1 Publication
  1. Vmax=27 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.5.1 Publication

GO - Molecular functioni

  • metal ion binding Source: EcoCyc
  • triphosphatase activity Source: EcoCyc

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG11603-MONOMER
MetaCyc:EG11603-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic triphosphatase (EC:3.6.1.25)
Short name:
PPPase
Alternative name(s):
ORFXE
Gene namesi
Name:ygiF
Ordered Locus Names:b3054, JW3026
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11603 ygiF

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene do not show decrease of specific PPPase activity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001694061 – 433Inorganic triphosphataseAdd BLAST433

Proteomic databases

PaxDbiP30871
PRIDEiP30871

Interactioni

Protein-protein interaction databases

BioGridi4259253, 8 interactors
DIPiDIP-12218N
IntActiP30871, 23 interactors
STRINGi316385.ECDH10B_3229

Structurei

Secondary structure

1433
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 11Combined sources10
Helixi13 – 15Combined sources3
Helixi16 – 23Combined sources8
Beta strandi29 – 42Combined sources14
Helixi47 – 50Combined sources4
Beta strandi54 – 60Combined sources7
Beta strandi63 – 69Combined sources7
Beta strandi73 – 75Combined sources3
Beta strandi78 – 92Combined sources15
Helixi95 – 97Combined sources3
Helixi100 – 102Combined sources3
Beta strandi103 – 107Combined sources5
Helixi112 – 115Combined sources4
Beta strandi117 – 133Combined sources17
Beta strandi136 – 149Combined sources14
Beta strandi152 – 166Combined sources15
Helixi168 – 178Combined sources11
Beta strandi181 – 186Combined sources6
Helixi191 – 199Combined sources9
Helixi221 – 244Combined sources24
Helixi250 – 267Combined sources18
Turni268 – 271Combined sources4
Helixi274 – 277Combined sources4
Helixi278 – 293Combined sources16
Helixi297 – 302Combined sources6
Helixi304 – 318Combined sources15
Turni319 – 322Combined sources4
Helixi323 – 325Combined sources3
Helixi328 – 334Combined sources7
Helixi338 – 357Combined sources20
Helixi366 – 368Combined sources3
Helixi369 – 382Combined sources14
Helixi388 – 407Combined sources20
Helixi410 – 422Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5A60X-ray1.82A1-433[»]
5A61X-ray1.50A1-433[»]
ProteinModelPortaliP30871
SMRiP30871
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 202CYTHPROSITE-ProRule annotationAdd BLAST201
Domaini218 – 433CHADPROSITE-ProRule annotationAdd BLAST216

Phylogenomic databases

eggNOGiENOG4105I7R Bacteria
COG3025 LUCA
HOGENOMiHOG000283528
InParanoidiP30871
KOiK18446
OMAiMPCDISK

Family and domain databases

InterProiView protein in InterPro
IPR007899 CHAD_dom
IPR033469 CYTH-like_dom_sf
IPR023577 CYTH_domain
PfamiView protein in Pfam
PF01928 CYTH, 1 hit
SMARTiView protein in SMART
SM01118 CYTH, 1 hit
SUPFAMiSSF55154 SSF55154, 1 hit
PROSITEiView protein in PROSITE
PS51708 CHAD, 1 hit
PS51707 CYTH, 1 hit

Sequencei

Sequence statusi: Complete.

P30871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQEIELKFI VNHSAVEALR DHLNTLGGEH HDPVQLLNIY YETPDNWLRG
60 70 80 90 100
HDMGLRIRGE NGRYEMTMKV AGRVTGGLHQ RPEYNVALSE PTLDLAQLPT
110 120 130 140 150
EVWPNGELPA DLASRVQPLF STDFYREKWL VAVDGSQIEI ALDQGEVKAG
160 170 180 190 200
EFAEPICELE LELLSGDTRA VLKLANQLVS QTGLRQGSLS KAARGYHLAQ
210 220 230 240 250
GNPAREIKPT TILHVAAKAD VEQGLEAALE LALAQWQYHE ELWVRGNDAA
260 270 280 290 300
KEQVLAAISL VRHTLMLFGG IVPRKASTHL RDLLTQCEAT IASAVSAVTA
310 320 330 340 350
VYSTETAMAK LALTEWLVSK AWQPFLDAKA QGKISDSFKR FADIHLSRHA
360 370 380 390 400
AELKSVFCQP LGDRYRDQLP RLTRDIDSIL LLAGYYDPVV AQAWLENWQG
410 420 430
LHHAIATGQR IEIEHFRNEA NNQEPFWLHS GKR
Length:433
Mass (Da):48,389
Last modified:July 1, 1993 - v1
Checksum:iF2CB1519123C0277
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21844 Genomic DNA Translation: CAA79891.1
U00096 Genomic DNA Translation: AAC76090.1
AP009048 Genomic DNA Translation: BAE77105.1
PIRiS37754
RefSeqiNP_417526.1, NC_000913.3
WP_000046281.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76090; AAC76090; b3054
BAE77105; BAE77105; BAE77105
GeneIDi947554
KEGGiecj:JW3026
eco:b3054
PATRICifig|1411691.4.peg.3677

Similar proteinsi

Entry informationi

Entry namei3PASE_ECOLI
AccessioniPrimary (citable) accession number: P30871
Secondary accession number(s): Q2M9F1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: March 28, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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