P30864 (YAFC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized HTH-type transcriptional regulator YafC

Gene names

Name:	yafC
Ordered Locus Names:	b0208, JW0198

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Sequence similarities

Contains 1 HTH lysR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Uncharacterized HTH-type transcriptional regulator YafC		PRO_0000105774
Regions
	Domain	1 – 60	60	HTH lysR-type
	DNA binding	20 – 39	20	H-T-H motif By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30864 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: E7179C36A2264BA9	FASTA	304	33,776
	10 20 30 40 50 60 MKATSEELAI FVSVVESGSF SRAAEQLGQA NSAVSRAVKK LEMKLGVSLL NRTTRQLSLT 70 80 90 100 110 120 EEGERYFRRV QSILQEMAAA ESEIMETRNT PRGLLRIDAA TPVVLHFLMP LIKPFRERYP 130 140 150 160 170 180 EVTLSLVSSE TIINLIERKV DVAIRAGTLT DSSLRARPLF NSYRKIIASP DYISRYGKPE 190 200 210 220 230 240 TIDDLKQHIC LGFTEPASLN TWPIARSDGQ LHEVKYGLSS NSGETLKQLC LSGNGIACLS 250 260 270 280 290 300 DYMIDKEIAR GELVELMADK VLPVEMPFSA VYYSDRAVST RIRAFIDFLS EHVKTAPGGA VREA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Nishimura K., Komine Y., Miyamoto K., Kitabatake M., Mathunaga F., Hisano T., Miki T., Inokuchi H. Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12650 Genomic DNA. Translation: BAA02171.1. U70214 Genomic DNA. Translation: AAB08630.1. U00096 Genomic DNA. Translation: AAC73313.1. AP009048 Genomic DNA. Translation: BAA77879.1.
PIR	JS0716.
RefSeq	NP_414744.1. NC_000913.2. YP_488505.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P30864.
SMR	P30864. Positions 1-293.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11210N.
IntAct	P30864. 6 interactions.
MINT	MINT-1323766.
STRING	511145.b0208.
Proteomic databases
PaxDb	P30864.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73313; AAC73313; b0208. BAA77879; BAA77879; BAA77879.
GeneID	12932794. 947507.
KEGG	ecj:Y75_p0199. eco:b0208.
PATRIC	32115529. VBIEscCol129921_0210.
Organism-specific databases
EchoBASE	EB1602.
EcoGene	EG11649. yafC.
Phylogenomic databases
eggNOG	COG0583.
HOGENOM	HOG000233519.
OMA	CLSDYMV.
ProtClustDB	CLSK879575.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11649-MONOMER. ECOL316407:JW0198-MONOMER.
Gene expression databases
Genevestigator	P30864.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR005119. LysR_subst-bd. IPR000847. Tscrpt_reg_HTH_LysR. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00126. HTH_1. 1 hit. PF03466. LysR_substrate. 1 hit. [Graphical view]
PRINTS	PR00039. HTHLYSR.
PROSITE	PS50931. HTH_LYSR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YAFC_ECOLI

Accession

Primary (citable) accession number: P30864

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program