Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2,5-diketo-D-gluconic acid reductase B

Gene

dkgB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG).

Miscellaneous

2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).

Catalytic activityi

2-dehydro-L-gulonate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.

pH dependencei

Optimum pH is 7.0.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei39Proton donorBy similarity1
Binding sitei97SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi179 – 231NADPBy similarityAdd BLAST53

GO - Molecular functioni

  • aldo-keto reductase (NADP) activity Source: EcoCyc
  • aryl-alcohol dehydrogenase (NADP+) activity Source: EcoCyc
  • methylglyoxal reductase (NADPH-dependent, acetol producing) Source: EcoCyc

GO - Biological processi

  • L-ascorbic acid biosynthetic process Source: UniProtKB-KW
  • methylglyoxal catabolic process Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processAscorbate biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-149.
MetaCyc:MONOMER0-149.
BRENDAi1.1.1.274. 2026.
SABIO-RKiP30863.

Names & Taxonomyi

Protein namesi
Recommended name:
2,5-diketo-D-gluconic acid reductase B (EC:1.1.1.346)
Short name:
2,5-DKG reductase B
Short name:
2,5-DKGR B
Short name:
25DKGR-B
Alternative name(s):
AKR5D
Gene namesi
Name:dkgB
Synonyms:yafB
Ordered Locus Names:b0207, JW0197
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11648. dkgB.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001246041 – 2672,5-diketo-D-gluconic acid reductase BAdd BLAST267

Proteomic databases

PaxDbiP30863.
PRIDEiP30863.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4261784. 5 interactors.
IntActiP30863. 1 interactor.
STRINGi316385.ECDH10B_0188.

Structurei

3D structure databases

ProteinModelPortaliP30863.
SMRiP30863.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiENOG4105CV8. Bacteria.
COG0656. LUCA.
HOGENOMiHOG000250272.
InParanoidiP30863.
KOiK06222.
PhylomeDBiP30863.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiView protein in InterPro
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
PfamiView protein in Pfam
PF00248. Aldo_ket_red. 1 hit.
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiView protein in PROSITE
PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P30863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIPAFGLGT FRLKDDVVIS SVITALELGY RAIDTAQIYD NEAAVGQAIA
60 70 80 90 100
ESGVPRHELY ITTKIWIENL SKDKLIPSLK ESLQKLRTDY VDLTLIHWPS
110 120 130 140 150
PNDEVSVEEF MQALLEAKKQ GLTREIGISN FTIPLMEKAI AAVGAENIAT
160 170 180 190 200
NQIELSPYLQ NRKVVAWAKQ HGIHITSYMT LAYGKALKDE VIARIAAKHN
210 220 230 240 250
ATPAQVILAW AMGEGYSVIP SSTKRKNLES NLKAQNLQLD AEDKKAIAAL
260
DCNDRLVSPE GLAPEWD
Length:267
Mass (Da):29,437
Last modified:April 27, 2001 - v2
Checksum:i265C7B1CC2D9C2E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194 – 195RI → AY in BAA02170 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12650 Genomic DNA. Translation: BAA02170.1.
U70214 Genomic DNA. Translation: AAB08629.1.
U00096 Genomic DNA. Translation: AAC73312.1.
AP009048 Genomic DNA. Translation: BAA77878.2.
V00336 Genomic DNA. Translation: CAA23619.1.
PIRiA64745.
RefSeqiNP_414743.1. NC_000913.3.
WP_000997010.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73312; AAC73312; b0207.
BAA77878; BAA77878; BAA77878.
GeneIDi944901.
KEGGiecj:JW0197.
eco:b0207.
PATRICifig|1411691.4.peg.2077.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDKGB_ECOLI
AccessioniPrimary (citable) accession number: P30863
Secondary accession number(s): P77777
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: April 27, 2001
Last modified: July 5, 2017
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families