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Protein

2,5-diketo-D-gluconic acid reductase B

Gene

dkgB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG).

Miscellaneous

2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).

Catalytic activityi

2-dehydro-L-gulonate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.

pH dependencei

Optimum pH is 7.0.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei39Proton donorBy similarity1
Binding sitei97SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi179 – 231NADPBy similarityAdd BLAST53

GO - Molecular functioni

  • aldo-keto reductase (NADP) activity Source: EcoCyc
  • aryl-alcohol dehydrogenase (NADP+) activity Source: EcoCyc
  • methylglyoxal reductase (NADPH-dependent, acetol producing) Source: EcoCyc

GO - Biological processi

  • L-ascorbic acid biosynthetic process Source: UniProtKB-KW
  • methylglyoxal catabolic process Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processAscorbate biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-149
MetaCyc:MONOMER0-149
BRENDAi1.1.1.274 2026
SABIO-RKiP30863

Names & Taxonomyi

Protein namesi
Recommended name:
2,5-diketo-D-gluconic acid reductase B (EC:1.1.1.346)
Short name:
2,5-DKG reductase B
Short name:
2,5-DKGR B
Short name:
25DKGR-B
Alternative name(s):
AKR5D
Gene namesi
Name:dkgB
Synonyms:yafB
Ordered Locus Names:b0207, JW0197
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11648 dkgB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001246041 – 2672,5-diketo-D-gluconic acid reductase BAdd BLAST267

Proteomic databases

PaxDbiP30863
PRIDEiP30863

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4261784, 5 interactors
IntActiP30863, 1 interactor
STRINGi316385.ECDH10B_0188

Structurei

3D structure databases

ProteinModelPortaliP30863
SMRiP30863
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiENOG4105CV8 Bacteria
COG0656 LUCA
HOGENOMiHOG000250272
InParanoidiP30863
KOiK06222
OMAiKIWTENL
PhylomeDBiP30863

Family and domain databases

CDDicd06660 Aldo_ket_red, 1 hit
Gene3Di3.20.20.100, 1 hit
InterProiView protein in InterPro
IPR018170 Aldo/ket_reductase_CS
IPR020471 Aldo/keto_reductase
IPR023210 NADP_OxRdtase_dom
IPR036812 NADP_OxRdtase_dom_sf
PfamiView protein in Pfam
PF00248 Aldo_ket_red, 1 hit
PIRSFiPIRSF000097 AKR, 1 hit
PRINTSiPR00069 ALDKETRDTASE
SUPFAMiSSF51430 SSF51430, 1 hit
PROSITEiView protein in PROSITE
PS00798 ALDOKETO_REDUCTASE_1, 1 hit
PS00062 ALDOKETO_REDUCTASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P30863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIPAFGLGT FRLKDDVVIS SVITALELGY RAIDTAQIYD NEAAVGQAIA
60 70 80 90 100
ESGVPRHELY ITTKIWIENL SKDKLIPSLK ESLQKLRTDY VDLTLIHWPS
110 120 130 140 150
PNDEVSVEEF MQALLEAKKQ GLTREIGISN FTIPLMEKAI AAVGAENIAT
160 170 180 190 200
NQIELSPYLQ NRKVVAWAKQ HGIHITSYMT LAYGKALKDE VIARIAAKHN
210 220 230 240 250
ATPAQVILAW AMGEGYSVIP SSTKRKNLES NLKAQNLQLD AEDKKAIAAL
260
DCNDRLVSPE GLAPEWD
Length:267
Mass (Da):29,437
Last modified:April 27, 2001 - v2
Checksum:i265C7B1CC2D9C2E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194 – 195RI → AY in BAA02170 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12650 Genomic DNA Translation: BAA02170.1
U70214 Genomic DNA Translation: AAB08629.1
U00096 Genomic DNA Translation: AAC73312.1
AP009048 Genomic DNA Translation: BAA77878.2
V00336 Genomic DNA Translation: CAA23619.1
PIRiA64745
RefSeqiNP_414743.1, NC_000913.3
WP_000997010.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73312; AAC73312; b0207
BAA77878; BAA77878; BAA77878
GeneIDi944901
KEGGiecj:JW0197
eco:b0207
PATRICifig|1411691.4.peg.2077

Entry informationi

Entry nameiDKGB_ECOLI
AccessioniPrimary (citable) accession number: P30863
Secondary accession number(s): P77777
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: April 27, 2001
Last modified: March 28, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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