Sensor protein EvgS precursor - Escherichia coli (strain K12)

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P30855 (EVGS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 128. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sensor protein EvgS
EC=2.7.13.3

Gene names

Name:	evgS
Ordered Locus Names:	b2370, JW2367

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	1197 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system EvgS/EvgA. Phosphorylates EvgA via a four-step phosphorelay in response to environmental signals.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Probable.
Post-translational modification	Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 HPt domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC protein autophosphorylation Inferred from direct assay Ref.7. Source: EcoCyc regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW histidine phosphotransfer kinase activity Inferred from mutant phenotype Ref.7. Source: EcoCyc phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro phosphorelay sensor kinase activity Inferred from direct assay Ref.7. Source: EcoCyc transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

Potential

Chain

22 – 1197

1176

Sensor protein EvgS

PRO_0000032371

Regions

Topological domain

22 – 325

304

Cytoplasmic Potential

Transmembrane

326 – 346

Helical; Potential

Topological domain

347 – 537

191

Periplasmic Potential

Transmembrane

538 – 558

Helical; Potential

Topological domain

559 – 1197

639

Cytoplasmic Potential

Domain

718 – 938

221

Histidine kinase

Domain

960 – 1074

115

Response regulatory

Domain

1098 – 1197

100

HPt

Amino acid modifications

Modified residue

721

Phosphohistidine; by autocatalysis By similarity

Modified residue

1009

4-aspartylphosphate By similarity

Modified residue

1137

Phosphohistidine By similarity

Natural variations

Natural variant

577

F → S in EvgS1; constitutively active.

Natural variant

701

E → G in EvgS4; constitutively active.

Experimental info

Sequence conflict

152

L → F Ref.1

Sequence conflict

152

L → F Ref.2

Sequence conflict

242 – 243

FF → PL Ref.1

Sequence conflict

242 – 243

FF → PL Ref.2

Sequence conflict

275

W → R Ref.1

Sequence conflict

275

W → R Ref.2

Sequence conflict

420 – 421

SQ → FE Ref.1

Sequence conflict

420 – 421

SQ → FE Ref.2

Sequence conflict

739

G → D Ref.1

Sequence conflict

739

G → D Ref.2

Sequence conflict

758

G → K Ref.1

Sequence conflict

758

G → K Ref.2

Sequence conflict

761

L → V Ref.1

Sequence conflict

761

L → V Ref.2

Sequence conflict

877

S → L Ref.1

Sequence conflict

877

S → L Ref.2

Sequence conflict

1045

R → H Ref.1

Sequence conflict

1045

R → H Ref.2

Sequence conflict

1074

H → Y Ref.1

Sequence conflict

1074

H → Y Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P30855 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: E8E1DE0F797B1278
Show »

FASTA

1,197

134,743

        10         20         30         40         50         60 
MKFLPYIFLL CCGLWSTISF ADEDYIEYRG ISSNNRVTLD PLRLSNKELR WLASKKNLVI 

        70         80         90        100        110        120 
AVHKSQTATL LHTDSQQRVR GINADYLNLL KRALNIKLTL REYADHQKAM DALAEGEVDI 

       130        140        150        160        170        180 
VLSHLVTSPP LNNDIAATKP LIITFPALVT TLHDSMRPLT SPKPVNIARV ANYPPDEVIH 

       190        200        210        220        230        240 
QSFPKATIIS FTNLYQALAS VSAGHNDYFI GSNIITSSMI SRYFTHSLNV VKYYNSPRQY 

       250        260        270        280        290        300 
NFFLTRKESV ILNEVLNRFV DALTNEVRYE VSQNWLDTGN LAFLNKPLEL TEHEKQWIKQ 

       310        320        330        340        350        360 
HPNLKVLENP YSPPYSMTDE NGSVRGVMGD ILNIITLQTG LNFSPITVSH NIHAGTQLSP 

       370        380        390        400        410        420 
GGWDIIPGAI YSEDRENNVL FAEAFITTPY VFVMQKAPDS EQTLKKGMKV AIPYYYELHS 

       430        440        450        460        470        480 
QLKEMYPEVE WIQVDNASAA FHKVKEGELD ALVATQLNSR YMIDHYYPNE LYHFLIPGVP 

       490        500        510        520        530        540 
NASLSFAFPR GEPELKDIIN KALNAIPPSE VLRLTEKWIK MPNVTIDTWD LYSEQFYIVT 

       550        560        570        580        590        600 
TLSVLLVGSS LLWGFYLLRS VRRRKVIQGD LENQISFRKA LSDSLPNPTY VVNWQGNVIS 

       610        620        630        640        650        660 
HNSAFEHYFT ADYYKNAMLP LENSDSPFKD VFSNAHEVTA ETKENRTIYT QVFEIDNGIE 

       670        680        690        700        710        720 
KRCINHWHTL CNLPASDNAV YICGWQDITE TRDLINALEV EKNKAIKATV AKSQFLATMS 

       730        740        750        760        770        780 
HEIRTPISSI MGFLELLSGS GLSKEQRVEA ISLAYATGQS LLGLIGEILD VDKIESGNYQ 

       790        800        810        820        830        840 
LQPQWVDIPT LVQNTCHSFG AIAASKSIAL SCSSTFPEHY LVKIDPQAFK QVLSNLLSNA 

       850        860        870        880        890        900 
LKFTTEGAVK ITTSLGHIDD NHAVIKMTIM DSGSGLSQEE QQQLFKRYSQ TSAGRQQTGS 

       910        920        930        940        950        960 
GLGLMICKEL IKNMQGDLSL ESHPGIGTTF TITIPVEISQ QVATVEAKAE QPITLPEKLS 

       970        980        990       1000       1010       1020 
ILIADDHPTN RLLLKRQLNL LGYDVDEATD GVQALHKVSM QHYDLLITDV NMPNMDGFEL 

      1030       1040       1050       1060       1070       1080 
TRKLREQNSS LPIWGLTANA QANEREKGLS CGMNLCLFKP LTLDVLKTHL SQLHQVAHIA 

      1090       1100       1110       1120       1130       1140 
PQYRHLDIEA LKNNTANDLQ LMQEILMTFQ HETHKDLPAA FQALEAGDNR TFHQCIHRIH 

      1150       1160       1170       1180       1190 
GAANILNLQK LINISHQLEI TPVSDDSKPE ILQLLNSVKE HIAELDQEIA VFCQKND

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Newly identified genes involved in the signal transduction of Escherichia coli K-12." Utsumi R., Katayama S., Taniguchi M., Horie T., Ikeda M., Igaki S., Nakagawa H., Miwa A., Tanabe H., Noda M. Gene 140:73-77(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Cloning and sequence analysis of the evgAS genes involved in signal transduction of Escherichia coli K-12." Utsumi R., Katayama S., Ikeda M., Igaki S., Nakagawa H., Miwa A., Taniguchi M., Noda M. Nucleic Acids Symp. Ser. 27:149-150(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12." Kato A., Ohnishi H., Yamamoto K., Furuta E., Tanabe H., Utsumi R. Biosci. Biotechnol. Biochem. 64:1203-1209(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (VARIANTS EVGS1 AND EVGS4). Strain: K12.
[4]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Specificity of the BvgAS and EvgAS phosphorelay is mediated by the C-terminal HPt domains of the sensor proteins." Perraud A.-L., Kimmel B., Weiss V., Gross R. Mol. Microbiol. 27:875-887(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14008 Genomic DNA. Translation: BAA03108.1. AF201840 Genomic DNA. Translation: AAF17563.1. AF201841 Genomic DNA. Translation: AAF17564.1. U00096 Genomic DNA. Translation: AAC75429.1. AP009048 Genomic DNA. Translation: BAA16241.1.
PIR	G65010.
RefSeq	NP_416871.1. NC_000913.2. YP_490612.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P30855.
SMR	P30855. Positions 45-275, 291-518, 686-935, 957-1104.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9545N.
IntAct	P30855. 2 interactions.
MINT	MINT-1288120.
STRING	511145.b2370.
Proteomic databases
PaxDb	P30855.
PRIDE	P30855.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75429; AAC75429; b2370. BAA16241; BAA16241; BAA16241.
GeneID	12931565. 946844.
KEGG	ecj:Y75_p2337. eco:b2370.
PATRIC	32120119. VBIEscCol129921_2468.
Organism-specific databases
EchoBASE	EB1567.
EcoGene	EG11610. evgS.
Phylogenomic databases
eggNOG	COG0784.
HOGENOM	HOG000122879.
KO	K07679.
OMA	SFAEAFI.
ProtClustDB	PRK09959.
Enzyme and pathway databases
BioCyc	EcoCyc:EVGS-MONOMER. ECOL316407:JW2367-MONOMER.
BRENDA	2.7.13.3. 2026.
Gene expression databases
Genevestigator	P30855.
Family and domain databases
Gene3D	1.10.287.130. 1 hit. 1.20.120.160. 1 hit. 3.30.565.10. 1 hit.
InterPro	IPR011006. CheY-like_superfamily. IPR003594. HATPase_ATP-bd. IPR001638. SBP_bac_3. IPR004358. Sig_transdc_His_kin-like_C. IPR008207. Sig_transdc_His_kin_Hpt_dom. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Pfam	PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF01627. Hpt. 1 hit. PF00072. Response_reg. 1 hit. PF00497. SBP_bac_3. 2 hits. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00073. HPT. 1 hit. SM00062. PBPb. 2 hits. SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF52172. CheY_like. 1 hit. SSF47384. His_kin_homodim. 1 hit. SSF47226. Hpt. 1 hit.
PROSITE	PS50109. HIS_KIN. 1 hit. PS50894. HPT. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EVGS_ECOLI

Accession

Primary (citable) accession number: P30855
Secondary accession number(s): P77644, Q9RF36, Q9RF37

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 128 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents