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P30855

- EVGS_ECOLI

UniProt

P30855 - EVGS_ECOLI

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Protein
Sensor protein EvgS
Gene
evgS, b2370, JW2367
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system EvgS/EvgA. Phosphorylates EvgA via a four-step phosphorelay in response to environmental signals.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. histidine phosphotransfer kinase activity Source: EcoCyc
  3. phosphorelay response regulator activity Source: InterPro
  4. phosphorelay sensor kinase activity Source: EcoCyc
  5. transporter activity Source: InterPro

GO - Biological processi

  1. phosphorelay signal transduction system Source: EcoCyc
  2. protein autophosphorylation Source: EcoCyc
  3. signal transduction by phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EVGS-MONOMER.
ECOL316407:JW2367-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein EvgS (EC:2.7.13.3)
Gene namesi
Name:evgS
Ordered Locus Names:b2370, JW2367
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11610. evgS.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 325304Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei326 – 34621Helical; Reviewed prediction
Add
BLAST
Topological domaini347 – 537191Periplasmic Reviewed prediction
Add
BLAST
Transmembranei538 – 55821Helical; Reviewed prediction
Add
BLAST
Topological domaini559 – 1197639Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 11971176Sensor protein EvgS
PRO_0000032371Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei721 – 7211Phosphohistidine; by autocatalysis By similarity
Modified residuei1009 – 100914-aspartylphosphate By similarity
Modified residuei1137 – 11371Phosphohistidine By similarity

Post-translational modificationi

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30855.
PRIDEiP30855.

Expressioni

Gene expression databases

GenevestigatoriP30855.

Interactioni

Protein-protein interaction databases

DIPiDIP-9545N.
IntActiP30855. 3 interactions.
MINTiMINT-1288120.
STRINGi511145.b2370.

Structurei

3D structure databases

ProteinModelPortaliP30855.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini718 – 938221Histidine kinase
Add
BLAST
Domaini960 – 1074115Response regulatory
Add
BLAST
Domaini1098 – 1197100HPt
Add
BLAST

Sequence similaritiesi

Contains 1 HPt domain.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0784.
HOGENOMiHOG000122879.
KOiK07679.
OMAiMVDHYLP.
OrthoDBiEOG6G4VQG.
PhylomeDBiP30855.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003661. EnvZ-like_dim/P.
IPR003594. HATPase_ATP-bd.
IPR001638. SBP_bac_3.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00072. Response_reg. 1 hit.
PF00497. SBP_bac_3. 2 hits.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00062. PBPb. 2 hits.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30855-1 [UniParc]FASTAAdd to Basket

« Hide

MKFLPYIFLL CCGLWSTISF ADEDYIEYRG ISSNNRVTLD PLRLSNKELR     50
WLASKKNLVI AVHKSQTATL LHTDSQQRVR GINADYLNLL KRALNIKLTL 100
REYADHQKAM DALAEGEVDI VLSHLVTSPP LNNDIAATKP LIITFPALVT 150
TLHDSMRPLT SPKPVNIARV ANYPPDEVIH QSFPKATIIS FTNLYQALAS 200
VSAGHNDYFI GSNIITSSMI SRYFTHSLNV VKYYNSPRQY NFFLTRKESV 250
ILNEVLNRFV DALTNEVRYE VSQNWLDTGN LAFLNKPLEL TEHEKQWIKQ 300
HPNLKVLENP YSPPYSMTDE NGSVRGVMGD ILNIITLQTG LNFSPITVSH 350
NIHAGTQLSP GGWDIIPGAI YSEDRENNVL FAEAFITTPY VFVMQKAPDS 400
EQTLKKGMKV AIPYYYELHS QLKEMYPEVE WIQVDNASAA FHKVKEGELD 450
ALVATQLNSR YMIDHYYPNE LYHFLIPGVP NASLSFAFPR GEPELKDIIN 500
KALNAIPPSE VLRLTEKWIK MPNVTIDTWD LYSEQFYIVT TLSVLLVGSS 550
LLWGFYLLRS VRRRKVIQGD LENQISFRKA LSDSLPNPTY VVNWQGNVIS 600
HNSAFEHYFT ADYYKNAMLP LENSDSPFKD VFSNAHEVTA ETKENRTIYT 650
QVFEIDNGIE KRCINHWHTL CNLPASDNAV YICGWQDITE TRDLINALEV 700
EKNKAIKATV AKSQFLATMS HEIRTPISSI MGFLELLSGS GLSKEQRVEA 750
ISLAYATGQS LLGLIGEILD VDKIESGNYQ LQPQWVDIPT LVQNTCHSFG 800
AIAASKSIAL SCSSTFPEHY LVKIDPQAFK QVLSNLLSNA LKFTTEGAVK 850
ITTSLGHIDD NHAVIKMTIM DSGSGLSQEE QQQLFKRYSQ TSAGRQQTGS 900
GLGLMICKEL IKNMQGDLSL ESHPGIGTTF TITIPVEISQ QVATVEAKAE 950
QPITLPEKLS ILIADDHPTN RLLLKRQLNL LGYDVDEATD GVQALHKVSM 1000
QHYDLLITDV NMPNMDGFEL TRKLREQNSS LPIWGLTANA QANEREKGLS 1050
CGMNLCLFKP LTLDVLKTHL SQLHQVAHIA PQYRHLDIEA LKNNTANDLQ 1100
LMQEILMTFQ HETHKDLPAA FQALEAGDNR TFHQCIHRIH GAANILNLQK 1150
LINISHQLEI TPVSDDSKPE ILQLLNSVKE HIAELDQEIA VFCQKND 1197
Length:1,197
Mass (Da):134,743
Last modified:November 1, 1997 - v2
Checksum:iE8E1DE0F797B1278
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti577 – 5771F → S in EvgS1; constitutively active.
Natural varianti701 – 7011E → G in EvgS4; constitutively active.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1521L → F1 Publication
Sequence conflicti152 – 1521L → F1 Publication
Sequence conflicti242 – 2432FF → PL1 Publication
Sequence conflicti242 – 2432FF → PL1 Publication
Sequence conflicti275 – 2751W → R1 Publication
Sequence conflicti275 – 2751W → R1 Publication
Sequence conflicti420 – 4212SQ → FE1 Publication
Sequence conflicti420 – 4212SQ → FE1 Publication
Sequence conflicti739 – 7391G → D1 Publication
Sequence conflicti739 – 7391G → D1 Publication
Sequence conflicti758 – 7581G → K1 Publication
Sequence conflicti758 – 7581G → K1 Publication
Sequence conflicti761 – 7611L → V1 Publication
Sequence conflicti761 – 7611L → V1 Publication
Sequence conflicti877 – 8771S → L1 Publication
Sequence conflicti877 – 8771S → L1 Publication
Sequence conflicti1045 – 10451R → H1 Publication
Sequence conflicti1045 – 10451R → H1 Publication
Sequence conflicti1074 – 10741H → Y1 Publication
Sequence conflicti1074 – 10741H → Y1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14008 Genomic DNA. Translation: BAA03108.1.
AF201840 Genomic DNA. Translation: AAF17563.1.
AF201841 Genomic DNA. Translation: AAF17564.1.
U00096 Genomic DNA. Translation: AAC75429.1.
AP009048 Genomic DNA. Translation: BAA16241.1.
PIRiG65010.
RefSeqiNP_416871.1. NC_000913.3.
YP_490612.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75429; AAC75429; b2370.
BAA16241; BAA16241; BAA16241.
GeneIDi12931565.
946844.
KEGGiecj:Y75_p2337.
eco:b2370.
PATRICi32120119. VBIEscCol129921_2468.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14008 Genomic DNA. Translation: BAA03108.1 .
AF201840 Genomic DNA. Translation: AAF17563.1 .
AF201841 Genomic DNA. Translation: AAF17564.1 .
U00096 Genomic DNA. Translation: AAC75429.1 .
AP009048 Genomic DNA. Translation: BAA16241.1 .
PIRi G65010.
RefSeqi NP_416871.1. NC_000913.3.
YP_490612.1. NC_007779.1.

3D structure databases

ProteinModelPortali P30855.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9545N.
IntActi P30855. 3 interactions.
MINTi MINT-1288120.
STRINGi 511145.b2370.

Proteomic databases

PaxDbi P30855.
PRIDEi P30855.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75429 ; AAC75429 ; b2370 .
BAA16241 ; BAA16241 ; BAA16241 .
GeneIDi 12931565.
946844.
KEGGi ecj:Y75_p2337.
eco:b2370.
PATRICi 32120119. VBIEscCol129921_2468.

Organism-specific databases

EchoBASEi EB1567.
EcoGenei EG11610. evgS.

Phylogenomic databases

eggNOGi COG0784.
HOGENOMi HOG000122879.
KOi K07679.
OMAi MVDHYLP.
OrthoDBi EOG6G4VQG.
PhylomeDBi P30855.

Enzyme and pathway databases

BioCyci EcoCyc:EVGS-MONOMER.
ECOL316407:JW2367-MONOMER.
BRENDAi 2.7.13.3. 2026.

Miscellaneous databases

PROi P30855.

Gene expression databases

Genevestigatori P30855.

Family and domain databases

Gene3Di 1.10.287.130. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProi IPR011006. CheY-like_superfamily.
IPR003661. EnvZ-like_dim/P.
IPR003594. HATPase_ATP-bd.
IPR001638. SBP_bac_3.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view ]
Pfami PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00072. Response_reg. 1 hit.
PF00497. SBP_bac_3. 2 hits.
[Graphical view ]
PRINTSi PR00344. BCTRLSENSOR.
SMARTi SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00062. PBPb. 2 hits.
SM00448. REC. 1 hit.
[Graphical view ]
SUPFAMi SSF47226. SSF47226. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEi PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Newly identified genes involved in the signal transduction of Escherichia coli K-12."
    Utsumi R., Katayama S., Taniguchi M., Horie T., Ikeda M., Igaki S., Nakagawa H., Miwa A., Tanabe H., Noda M.
    Gene 140:73-77(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Cloning and sequence analysis of the evgAS genes involved in signal transduction of Escherichia coli K-12."
    Utsumi R., Katayama S., Ikeda M., Igaki S., Nakagawa H., Miwa A., Taniguchi M., Noda M.
    Nucleic Acids Symp. Ser. 27:149-150(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12."
    Kato A., Ohnishi H., Yamamoto K., Furuta E., Tanabe H., Utsumi R.
    Biosci. Biotechnol. Biochem. 64:1203-1209(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (VARIANTS EVGS1 AND EVGS4).
    Strain: K12.
  4. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Specificity of the BvgAS and EvgAS phosphorelay is mediated by the C-terminal HPt domains of the sensor proteins."
    Perraud A.-L., Kimmel B., Weiss V., Gross R.
    Mol. Microbiol. 27:875-887(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiEVGS_ECOLI
AccessioniPrimary (citable) accession number: P30855
Secondary accession number(s): P77644, Q9RF36, Q9RF37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi