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Protein

Sensor protein EvgS

Gene

evgS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system EvgS/EvgA. Phosphorylates EvgA via a four-step phosphorelay in response to environmental signals.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histidine phosphotransfer kinase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • peptidyl-histidine phosphorylation Source: GO_Central
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
  • signal transduction by protein phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EVGS-MONOMER.
ECOL316407:JW2367-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein EvgS (EC:2.7.13.3)
Gene namesi
Name:evgS
Ordered Locus Names:b2370, JW2367
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11610. evgS.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 325304CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei326 – 34621HelicalSequence AnalysisAdd
BLAST
Topological domaini347 – 537191PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei538 – 55821HelicalSequence AnalysisAdd
BLAST
Topological domaini559 – 1197639CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 11971176Sensor protein EvgSPRO_0000032371Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei721 – 7211Phosphohistidine; by autocatalysisPROSITE-ProRule annotation
Modified residuei1009 – 100914-aspartylphosphatePROSITE-ProRule annotation
Modified residuei1137 – 11371PhosphohistidinePROSITE-ProRule annotation

Post-translational modificationi

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30855.
PRIDEiP30855.

Interactioni

Protein-protein interaction databases

DIPiDIP-9545N.
IntActiP30855. 3 interactions.
MINTiMINT-1288120.
STRINGi511145.b2370.

Structurei

3D structure databases

ProteinModelPortaliP30855.
SMRiP30855. Positions 45-275, 291-518, 641-935, 957-1104.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini718 – 938221Histidine kinasePROSITE-ProRule annotationAdd
BLAST
Domaini960 – 1074115Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini1098 – 1197100HPtPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 HPt domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0784.
HOGENOMiHOG000122879.
InParanoidiP30855.
KOiK07679.
OMAiFMRVLID.
OrthoDBiEOG6G4VQG.
PhylomeDBiP30855.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003661. EnvZ-like_dim/P.
IPR003594. HATPase_C.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR001638. Solute-binding_3/MltF_N.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00062. PBPb. 2 hits.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLPYIFLL CCGLWSTISF ADEDYIEYRG ISSNNRVTLD PLRLSNKELR
60 70 80 90 100
WLASKKNLVI AVHKSQTATL LHTDSQQRVR GINADYLNLL KRALNIKLTL
110 120 130 140 150
REYADHQKAM DALAEGEVDI VLSHLVTSPP LNNDIAATKP LIITFPALVT
160 170 180 190 200
TLHDSMRPLT SPKPVNIARV ANYPPDEVIH QSFPKATIIS FTNLYQALAS
210 220 230 240 250
VSAGHNDYFI GSNIITSSMI SRYFTHSLNV VKYYNSPRQY NFFLTRKESV
260 270 280 290 300
ILNEVLNRFV DALTNEVRYE VSQNWLDTGN LAFLNKPLEL TEHEKQWIKQ
310 320 330 340 350
HPNLKVLENP YSPPYSMTDE NGSVRGVMGD ILNIITLQTG LNFSPITVSH
360 370 380 390 400
NIHAGTQLSP GGWDIIPGAI YSEDRENNVL FAEAFITTPY VFVMQKAPDS
410 420 430 440 450
EQTLKKGMKV AIPYYYELHS QLKEMYPEVE WIQVDNASAA FHKVKEGELD
460 470 480 490 500
ALVATQLNSR YMIDHYYPNE LYHFLIPGVP NASLSFAFPR GEPELKDIIN
510 520 530 540 550
KALNAIPPSE VLRLTEKWIK MPNVTIDTWD LYSEQFYIVT TLSVLLVGSS
560 570 580 590 600
LLWGFYLLRS VRRRKVIQGD LENQISFRKA LSDSLPNPTY VVNWQGNVIS
610 620 630 640 650
HNSAFEHYFT ADYYKNAMLP LENSDSPFKD VFSNAHEVTA ETKENRTIYT
660 670 680 690 700
QVFEIDNGIE KRCINHWHTL CNLPASDNAV YICGWQDITE TRDLINALEV
710 720 730 740 750
EKNKAIKATV AKSQFLATMS HEIRTPISSI MGFLELLSGS GLSKEQRVEA
760 770 780 790 800
ISLAYATGQS LLGLIGEILD VDKIESGNYQ LQPQWVDIPT LVQNTCHSFG
810 820 830 840 850
AIAASKSIAL SCSSTFPEHY LVKIDPQAFK QVLSNLLSNA LKFTTEGAVK
860 870 880 890 900
ITTSLGHIDD NHAVIKMTIM DSGSGLSQEE QQQLFKRYSQ TSAGRQQTGS
910 920 930 940 950
GLGLMICKEL IKNMQGDLSL ESHPGIGTTF TITIPVEISQ QVATVEAKAE
960 970 980 990 1000
QPITLPEKLS ILIADDHPTN RLLLKRQLNL LGYDVDEATD GVQALHKVSM
1010 1020 1030 1040 1050
QHYDLLITDV NMPNMDGFEL TRKLREQNSS LPIWGLTANA QANEREKGLS
1060 1070 1080 1090 1100
CGMNLCLFKP LTLDVLKTHL SQLHQVAHIA PQYRHLDIEA LKNNTANDLQ
1110 1120 1130 1140 1150
LMQEILMTFQ HETHKDLPAA FQALEAGDNR TFHQCIHRIH GAANILNLQK
1160 1170 1180 1190
LINISHQLEI TPVSDDSKPE ILQLLNSVKE HIAELDQEIA VFCQKND
Length:1,197
Mass (Da):134,743
Last modified:November 1, 1997 - v2
Checksum:iE8E1DE0F797B1278
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1521L → F (PubMed:8125343).Curated
Sequence conflicti152 – 1521L → F (PubMed:1289796).Curated
Sequence conflicti242 – 2432FF → PL (PubMed:8125343).Curated
Sequence conflicti242 – 2432FF → PL (PubMed:1289796).Curated
Sequence conflicti275 – 2751W → R (PubMed:8125343).Curated
Sequence conflicti275 – 2751W → R (PubMed:1289796).Curated
Sequence conflicti420 – 4212SQ → FE (PubMed:8125343).Curated
Sequence conflicti420 – 4212SQ → FE (PubMed:1289796).Curated
Sequence conflicti739 – 7391G → D (PubMed:8125343).Curated
Sequence conflicti739 – 7391G → D (PubMed:1289796).Curated
Sequence conflicti758 – 7581G → K (PubMed:8125343).Curated
Sequence conflicti758 – 7581G → K (PubMed:1289796).Curated
Sequence conflicti761 – 7611L → V (PubMed:8125343).Curated
Sequence conflicti761 – 7611L → V (PubMed:1289796).Curated
Sequence conflicti877 – 8771S → L (PubMed:8125343).Curated
Sequence conflicti877 – 8771S → L (PubMed:1289796).Curated
Sequence conflicti1045 – 10451R → H (PubMed:8125343).Curated
Sequence conflicti1045 – 10451R → H (PubMed:1289796).Curated
Sequence conflicti1074 – 10741H → Y (PubMed:8125343).Curated
Sequence conflicti1074 – 10741H → Y (PubMed:1289796).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti577 – 5771F → S in EvgS1; constitutively active.
Natural varianti701 – 7011E → G in EvgS4; constitutively active.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14008 Genomic DNA. Translation: BAA03108.1.
AF201840 Genomic DNA. Translation: AAF17563.1.
AF201841 Genomic DNA. Translation: AAF17564.1.
U00096 Genomic DNA. Translation: AAC75429.1.
AP009048 Genomic DNA. Translation: BAA16241.1.
PIRiG65010.
RefSeqiNP_416871.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75429; AAC75429; b2370.
BAA16241; BAA16241; BAA16241.
GeneIDi946844.
KEGGiecj:Y75_p2337.
eco:b2370.
PATRICi32120119. VBIEscCol129921_2468.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14008 Genomic DNA. Translation: BAA03108.1.
AF201840 Genomic DNA. Translation: AAF17563.1.
AF201841 Genomic DNA. Translation: AAF17564.1.
U00096 Genomic DNA. Translation: AAC75429.1.
AP009048 Genomic DNA. Translation: BAA16241.1.
PIRiG65010.
RefSeqiNP_416871.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP30855.
SMRiP30855. Positions 45-275, 291-518, 641-935, 957-1104.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9545N.
IntActiP30855. 3 interactions.
MINTiMINT-1288120.
STRINGi511145.b2370.

Proteomic databases

PaxDbiP30855.
PRIDEiP30855.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75429; AAC75429; b2370.
BAA16241; BAA16241; BAA16241.
GeneIDi946844.
KEGGiecj:Y75_p2337.
eco:b2370.
PATRICi32120119. VBIEscCol129921_2468.

Organism-specific databases

EchoBASEiEB1567.
EcoGeneiEG11610. evgS.

Phylogenomic databases

eggNOGiCOG0784.
HOGENOMiHOG000122879.
InParanoidiP30855.
KOiK07679.
OMAiFMRVLID.
OrthoDBiEOG6G4VQG.
PhylomeDBiP30855.

Enzyme and pathway databases

BioCyciEcoCyc:EVGS-MONOMER.
ECOL316407:JW2367-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

PROiP30855.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003661. EnvZ-like_dim/P.
IPR003594. HATPase_C.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR001638. Solute-binding_3/MltF_N.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00062. PBPb. 2 hits.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Newly identified genes involved in the signal transduction of Escherichia coli K-12."
    Utsumi R., Katayama S., Taniguchi M., Horie T., Ikeda M., Igaki S., Nakagawa H., Miwa A., Tanabe H., Noda M.
    Gene 140:73-77(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Cloning and sequence analysis of the evgAS genes involved in signal transduction of Escherichia coli K-12."
    Utsumi R., Katayama S., Ikeda M., Igaki S., Nakagawa H., Miwa A., Taniguchi M., Noda M.
    Nucleic Acids Symp. Ser. 27:149-150(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12."
    Kato A., Ohnishi H., Yamamoto K., Furuta E., Tanabe H., Utsumi R.
    Biosci. Biotechnol. Biochem. 64:1203-1209(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (VARIANTS EVGS1 AND EVGS4).
    Strain: K12.
  4. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Specificity of the BvgAS and EvgAS phosphorelay is mediated by the C-terminal HPt domains of the sensor proteins."
    Perraud A.-L., Kimmel B., Weiss V., Gross R.
    Mol. Microbiol. 27:875-887(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiEVGS_ECOLI
AccessioniPrimary (citable) accession number: P30855
Secondary accession number(s): P77644, Q9RF36, Q9RF37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.