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P30855

- EVGS_ECOLI

UniProt

P30855 - EVGS_ECOLI

Protein

Sensor protein EvgS

Gene

evgS

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Member of the two-component regulatory system EvgS/EvgA. Phosphorylates EvgA via a four-step phosphorelay in response to environmental signals.

    Catalytic activityi

    ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. histidine phosphotransfer kinase activity Source: EcoCyc
    3. phosphorelay response regulator activity Source: InterPro
    4. phosphorelay sensor kinase activity Source: EcoCyc
    5. transporter activity Source: InterPro

    GO - Biological processi

    1. phosphorelay signal transduction system Source: EcoCyc
    2. protein autophosphorylation Source: EcoCyc
    3. signal transduction by phosphorylation Source: GOC

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Two-component regulatory system

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EVGS-MONOMER.
    ECOL316407:JW2367-MONOMER.
    BRENDAi2.7.13.3. 2026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sensor protein EvgS (EC:2.7.13.3)
    Gene namesi
    Name:evgS
    Ordered Locus Names:b2370, JW2367
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11610. evgS.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 11971176Sensor protein EvgSPRO_0000032371Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei721 – 7211Phosphohistidine; by autocatalysisPROSITE-ProRule annotation
    Modified residuei1009 – 100914-aspartylphosphatePROSITE-ProRule annotation
    Modified residuei1137 – 11371PhosphohistidinePROSITE-ProRule annotation

    Post-translational modificationi

    Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP30855.
    PRIDEiP30855.

    Expressioni

    Gene expression databases

    GenevestigatoriP30855.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-9545N.
    IntActiP30855. 3 interactions.
    MINTiMINT-1288120.
    STRINGi511145.b2370.

    Structurei

    3D structure databases

    ProteinModelPortaliP30855.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini22 – 325304CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini347 – 537191PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini559 – 1197639CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei326 – 34621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei538 – 55821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini718 – 938221Histidine kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini960 – 1074115Response regulatoryPROSITE-ProRule annotationAdd
    BLAST
    Domaini1098 – 1197100HPtPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 histidine kinase domain.PROSITE-ProRule annotation
    Contains 1 HPt domain.PROSITE-ProRule annotation
    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0784.
    HOGENOMiHOG000122879.
    KOiK07679.
    OMAiMVDHYLP.
    OrthoDBiEOG6G4VQG.
    PhylomeDBiP30855.

    Family and domain databases

    Gene3Di1.10.287.130. 1 hit.
    1.20.120.160. 1 hit.
    3.30.565.10. 1 hit.
    InterProiIPR011006. CheY-like_superfamily.
    IPR003661. EnvZ-like_dim/P.
    IPR003594. HATPase_ATP-bd.
    IPR001638. SBP_bac_3.
    IPR004358. Sig_transdc_His_kin-like_C.
    IPR008207. Sig_transdc_His_kin_Hpt_dom.
    IPR005467. Sig_transdc_His_kinase_core.
    IPR009082. Sig_transdc_His_kinase_dimeric.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view]
    PfamiPF02518. HATPase_c. 1 hit.
    PF00512. HisKA. 1 hit.
    PF01627. Hpt. 1 hit.
    PF00072. Response_reg. 1 hit.
    PF00497. SBP_bac_3. 2 hits.
    [Graphical view]
    PRINTSiPR00344. BCTRLSENSOR.
    SMARTiSM00387. HATPase_c. 1 hit.
    SM00388. HisKA. 1 hit.
    SM00073. HPT. 1 hit.
    SM00062. PBPb. 2 hits.
    SM00448. REC. 1 hit.
    [Graphical view]
    SUPFAMiSSF47226. SSF47226. 1 hit.
    SSF47384. SSF47384. 1 hit.
    SSF52172. SSF52172. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEiPS50109. HIS_KIN. 1 hit.
    PS50894. HPT. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30855-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFLPYIFLL CCGLWSTISF ADEDYIEYRG ISSNNRVTLD PLRLSNKELR     50
    WLASKKNLVI AVHKSQTATL LHTDSQQRVR GINADYLNLL KRALNIKLTL 100
    REYADHQKAM DALAEGEVDI VLSHLVTSPP LNNDIAATKP LIITFPALVT 150
    TLHDSMRPLT SPKPVNIARV ANYPPDEVIH QSFPKATIIS FTNLYQALAS 200
    VSAGHNDYFI GSNIITSSMI SRYFTHSLNV VKYYNSPRQY NFFLTRKESV 250
    ILNEVLNRFV DALTNEVRYE VSQNWLDTGN LAFLNKPLEL TEHEKQWIKQ 300
    HPNLKVLENP YSPPYSMTDE NGSVRGVMGD ILNIITLQTG LNFSPITVSH 350
    NIHAGTQLSP GGWDIIPGAI YSEDRENNVL FAEAFITTPY VFVMQKAPDS 400
    EQTLKKGMKV AIPYYYELHS QLKEMYPEVE WIQVDNASAA FHKVKEGELD 450
    ALVATQLNSR YMIDHYYPNE LYHFLIPGVP NASLSFAFPR GEPELKDIIN 500
    KALNAIPPSE VLRLTEKWIK MPNVTIDTWD LYSEQFYIVT TLSVLLVGSS 550
    LLWGFYLLRS VRRRKVIQGD LENQISFRKA LSDSLPNPTY VVNWQGNVIS 600
    HNSAFEHYFT ADYYKNAMLP LENSDSPFKD VFSNAHEVTA ETKENRTIYT 650
    QVFEIDNGIE KRCINHWHTL CNLPASDNAV YICGWQDITE TRDLINALEV 700
    EKNKAIKATV AKSQFLATMS HEIRTPISSI MGFLELLSGS GLSKEQRVEA 750
    ISLAYATGQS LLGLIGEILD VDKIESGNYQ LQPQWVDIPT LVQNTCHSFG 800
    AIAASKSIAL SCSSTFPEHY LVKIDPQAFK QVLSNLLSNA LKFTTEGAVK 850
    ITTSLGHIDD NHAVIKMTIM DSGSGLSQEE QQQLFKRYSQ TSAGRQQTGS 900
    GLGLMICKEL IKNMQGDLSL ESHPGIGTTF TITIPVEISQ QVATVEAKAE 950
    QPITLPEKLS ILIADDHPTN RLLLKRQLNL LGYDVDEATD GVQALHKVSM 1000
    QHYDLLITDV NMPNMDGFEL TRKLREQNSS LPIWGLTANA QANEREKGLS 1050
    CGMNLCLFKP LTLDVLKTHL SQLHQVAHIA PQYRHLDIEA LKNNTANDLQ 1100
    LMQEILMTFQ HETHKDLPAA FQALEAGDNR TFHQCIHRIH GAANILNLQK 1150
    LINISHQLEI TPVSDDSKPE ILQLLNSVKE HIAELDQEIA VFCQKND 1197
    Length:1,197
    Mass (Da):134,743
    Last modified:November 1, 1997 - v2
    Checksum:iE8E1DE0F797B1278
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti152 – 1521L → F(PubMed:8125343)Curated
    Sequence conflicti152 – 1521L → F(PubMed:1289796)Curated
    Sequence conflicti242 – 2432FF → PL(PubMed:8125343)Curated
    Sequence conflicti242 – 2432FF → PL(PubMed:1289796)Curated
    Sequence conflicti275 – 2751W → R(PubMed:8125343)Curated
    Sequence conflicti275 – 2751W → R(PubMed:1289796)Curated
    Sequence conflicti420 – 4212SQ → FE(PubMed:8125343)Curated
    Sequence conflicti420 – 4212SQ → FE(PubMed:1289796)Curated
    Sequence conflicti739 – 7391G → D(PubMed:8125343)Curated
    Sequence conflicti739 – 7391G → D(PubMed:1289796)Curated
    Sequence conflicti758 – 7581G → K(PubMed:8125343)Curated
    Sequence conflicti758 – 7581G → K(PubMed:1289796)Curated
    Sequence conflicti761 – 7611L → V(PubMed:8125343)Curated
    Sequence conflicti761 – 7611L → V(PubMed:1289796)Curated
    Sequence conflicti877 – 8771S → L(PubMed:8125343)Curated
    Sequence conflicti877 – 8771S → L(PubMed:1289796)Curated
    Sequence conflicti1045 – 10451R → H(PubMed:8125343)Curated
    Sequence conflicti1045 – 10451R → H(PubMed:1289796)Curated
    Sequence conflicti1074 – 10741H → Y(PubMed:8125343)Curated
    Sequence conflicti1074 – 10741H → Y(PubMed:1289796)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti577 – 5771F → S in EvgS1; constitutively active.
    Natural varianti701 – 7011E → G in EvgS4; constitutively active.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14008 Genomic DNA. Translation: BAA03108.1.
    AF201840 Genomic DNA. Translation: AAF17563.1.
    AF201841 Genomic DNA. Translation: AAF17564.1.
    U00096 Genomic DNA. Translation: AAC75429.1.
    AP009048 Genomic DNA. Translation: BAA16241.1.
    PIRiG65010.
    RefSeqiNP_416871.1. NC_000913.3.
    YP_490612.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75429; AAC75429; b2370.
    BAA16241; BAA16241; BAA16241.
    GeneIDi12931565.
    946844.
    KEGGiecj:Y75_p2337.
    eco:b2370.
    PATRICi32120119. VBIEscCol129921_2468.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14008 Genomic DNA. Translation: BAA03108.1 .
    AF201840 Genomic DNA. Translation: AAF17563.1 .
    AF201841 Genomic DNA. Translation: AAF17564.1 .
    U00096 Genomic DNA. Translation: AAC75429.1 .
    AP009048 Genomic DNA. Translation: BAA16241.1 .
    PIRi G65010.
    RefSeqi NP_416871.1. NC_000913.3.
    YP_490612.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P30855.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9545N.
    IntActi P30855. 3 interactions.
    MINTi MINT-1288120.
    STRINGi 511145.b2370.

    Proteomic databases

    PaxDbi P30855.
    PRIDEi P30855.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75429 ; AAC75429 ; b2370 .
    BAA16241 ; BAA16241 ; BAA16241 .
    GeneIDi 12931565.
    946844.
    KEGGi ecj:Y75_p2337.
    eco:b2370.
    PATRICi 32120119. VBIEscCol129921_2468.

    Organism-specific databases

    EchoBASEi EB1567.
    EcoGenei EG11610. evgS.

    Phylogenomic databases

    eggNOGi COG0784.
    HOGENOMi HOG000122879.
    KOi K07679.
    OMAi MVDHYLP.
    OrthoDBi EOG6G4VQG.
    PhylomeDBi P30855.

    Enzyme and pathway databases

    BioCyci EcoCyc:EVGS-MONOMER.
    ECOL316407:JW2367-MONOMER.
    BRENDAi 2.7.13.3. 2026.

    Miscellaneous databases

    PROi P30855.

    Gene expression databases

    Genevestigatori P30855.

    Family and domain databases

    Gene3Di 1.10.287.130. 1 hit.
    1.20.120.160. 1 hit.
    3.30.565.10. 1 hit.
    InterProi IPR011006. CheY-like_superfamily.
    IPR003661. EnvZ-like_dim/P.
    IPR003594. HATPase_ATP-bd.
    IPR001638. SBP_bac_3.
    IPR004358. Sig_transdc_His_kin-like_C.
    IPR008207. Sig_transdc_His_kin_Hpt_dom.
    IPR005467. Sig_transdc_His_kinase_core.
    IPR009082. Sig_transdc_His_kinase_dimeric.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view ]
    Pfami PF02518. HATPase_c. 1 hit.
    PF00512. HisKA. 1 hit.
    PF01627. Hpt. 1 hit.
    PF00072. Response_reg. 1 hit.
    PF00497. SBP_bac_3. 2 hits.
    [Graphical view ]
    PRINTSi PR00344. BCTRLSENSOR.
    SMARTi SM00387. HATPase_c. 1 hit.
    SM00388. HisKA. 1 hit.
    SM00073. HPT. 1 hit.
    SM00062. PBPb. 2 hits.
    SM00448. REC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47226. SSF47226. 1 hit.
    SSF47384. SSF47384. 1 hit.
    SSF52172. SSF52172. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEi PS50109. HIS_KIN. 1 hit.
    PS50894. HPT. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Newly identified genes involved in the signal transduction of Escherichia coli K-12."
      Utsumi R., Katayama S., Taniguchi M., Horie T., Ikeda M., Igaki S., Nakagawa H., Miwa A., Tanabe H., Noda M.
      Gene 140:73-77(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Cloning and sequence analysis of the evgAS genes involved in signal transduction of Escherichia coli K-12."
      Utsumi R., Katayama S., Ikeda M., Igaki S., Nakagawa H., Miwa A., Taniguchi M., Noda M.
      Nucleic Acids Symp. Ser. 27:149-150(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. "Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12."
      Kato A., Ohnishi H., Yamamoto K., Furuta E., Tanabe H., Utsumi R.
      Biosci. Biotechnol. Biochem. 64:1203-1209(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (VARIANTS EVGS1 AND EVGS4).
      Strain: K12.
    4. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    7. "Specificity of the BvgAS and EvgAS phosphorelay is mediated by the C-terminal HPt domains of the sensor proteins."
      Perraud A.-L., Kimmel B., Weiss V., Gross R.
      Mol. Microbiol. 27:875-887(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiEVGS_ECOLI
    AccessioniPrimary (citable) accession number: P30855
    Secondary accession number(s): P77644, Q9RF36, Q9RF37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3