Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribonuclease R

Gene

rnr

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs (By similarity). Required for the expression of virulence genes on the large plasmid of S.flexneri at the post-transcriptional level.UniRule annotation

Catalytic activityi

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Virulence

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease RUniRule annotation (EC:3.1.13.1UniRule annotation)
Short name:
RNase RUniRule annotation
Alternative name(s):
Protein VacB
Gene namesi
Name:rnrUniRule annotation
Synonyms:vacB
Ordered Locus Names:SF4334, S4602
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001664111 – 813Ribonuclease RAdd BLAST813

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei544N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP30851.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi198214.SF4334.

Structurei

3D structure databases

ProteinModelPortaliP30851.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini644 – 725S1 motifUniRule annotationAdd BLAST82

Sequence similaritiesi

Belongs to the RNR ribonuclease family. RNase R subfamily.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C40. Bacteria.
COG0557. LUCA.
HOGENOMiHOG000071120.
KOiK12573.

Family and domain databases

HAMAPiMF_01895. RNase_R. 1 hit.
InterProiIPR011129. Cold_shock_prot.
IPR012340. NA-bd_OB-fold.
IPR013223. RNase_B_OB_dom.
IPR022966. RNase_II/R_CS.
IPR004476. RNase_II/RNase_R.
IPR011805. RNase_R.
IPR013668. RNase_R_HTH_12.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF08461. HTH_12. 1 hit.
PF08206. OB_RNB. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00357. CSP. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 4 hits.
TIGRFAMsiTIGR00358. 3_prime_RNase. 1 hit.
TIGR02063. RNase_R. 1 hit.
PROSITEiPS01175. RIBONUCLEASE_II. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30851-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQDPFQERE AEKYANPIPS REFILEHLTK REKPASRDEL AVELHIEGEE
60 70 80 90 100
QLEGLRRRLR AMERDGQLVF TRRQCYALPE RLDLVKGTVI GHRDGYGFLR
110 120 130 140 150
VEGRKDDLYL SSEQMKTCIH GDQVLAQPLG ADRKGRREAR IVRVLVPKTS
160 170 180 190 200
QIVGRYFTEA GVGFVVPDDS RLSFDILIPP DQIMGARMGF VVVVELTQRP
210 220 230 240 250
TRRTKAVGKI VEVLGDNMGT GMAVDIALRT HEIPYIWPQA VEQQVAGLKE
260 270 280 290 300
EVPEEAKAGR VDLRDLPLVT IDGEDARDFD DAVYCEKKRG GGWRLWVAIA
310 320 330 340 350
DVSYYVRPPT PLDREARNRG TSVYFPSQVI PMLPEVLSNG LCSLNPQVDR
360 370 380 390 400
LCMVCEMTVS SKGRLTGYKF YEAVMSSHAR LTYTKVWHIL QGDQDLREQY
410 420 430 440 450
APLVKHLEEL HNLYKVLDKA REERGGISFE SEEAKFIFNA ERRIERIEQT
460 470 480 490 500
QRNDAHKLIE ECMILANISA ARFVEKAKEP ALFRIHDKPS TEAITSFRSV
510 520 530 540 550
LAELGLELPG GNKPEPRDYA ELLESVADRP DAEMLQTMLL RSMKQAIYDP
560 570 580 590 600
ENRGHFGLAL QSYAHFTSPI RRYPDLTLHR AIKYLLAKEQ GHQGNTTETG
610 620 630 640 650
GYHYSMEEML QLGQHCSMAE RRADEATRDV ADWLKCDFML DQVGNVFKGV
660 670 680 690 700
ISSVTGFGFF VRLDDLFIDG LVHVSSLDND YYRFDQVGQR LMGESSGQTY
710 720 730 740 750
RLGDRVEVRV EAVNMDERKI DFSLISSERA PRNVGKTARE KAKKGDAGKK
760 770 780 790 800
GGKRCQVGKK VNFEPDSAFR GEKKTKPKAA KKDARKAKKP SAKTQKIAAA
810
TKAKRAAKKK VAE
Length:813
Mass (Da):92,066
Last modified:January 10, 2003 - v4
Checksum:i759B6D1B40C24494
GO

Sequence cautioni

The sequence AAN45751 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAP19534 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAA01777 differs from that shown. Several sequencing errors.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti520 – 521AE → GQ in BAA01777 (PubMed:1400189).Curated2
Sequence conflicti624D → E in BAA01777 (PubMed:1400189).Curated1
Sequence conflicti628R → G in BAA01777 (PubMed:1400189).Curated1
Sequence conflicti718R → A in BAA01777 (PubMed:1400189).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN45751.1. Different initiation.
AE014073 Genomic DNA. Translation: AAP19534.1. Different initiation.
D11024 Genomic DNA. Translation: BAA01777.1. Frameshift.
RefSeqiNP_710044.3. NC_004337.2.
WP_000076311.1. NZ_LVJC01000059.1.

Genome annotation databases

EnsemblBacteriaiAAN45751; AAN45751; SF4334.
AAP19534; AAP19534; S4602.
GeneIDi1025395.
KEGGisfl:SF4334.
sft:NCTC1_04709.
sfx:S4602.
PATRICi18711246. VBIShiFle31049_5021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN45751.1. Different initiation.
AE014073 Genomic DNA. Translation: AAP19534.1. Different initiation.
D11024 Genomic DNA. Translation: BAA01777.1. Frameshift.
RefSeqiNP_710044.3. NC_004337.2.
WP_000076311.1. NZ_LVJC01000059.1.

3D structure databases

ProteinModelPortaliP30851.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF4334.

Proteomic databases

PaxDbiP30851.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN45751; AAN45751; SF4334.
AAP19534; AAP19534; S4602.
GeneIDi1025395.
KEGGisfl:SF4334.
sft:NCTC1_04709.
sfx:S4602.
PATRICi18711246. VBIShiFle31049_5021.

Phylogenomic databases

eggNOGiENOG4105C40. Bacteria.
COG0557. LUCA.
HOGENOMiHOG000071120.
KOiK12573.

Family and domain databases

HAMAPiMF_01895. RNase_R. 1 hit.
InterProiIPR011129. Cold_shock_prot.
IPR012340. NA-bd_OB-fold.
IPR013223. RNase_B_OB_dom.
IPR022966. RNase_II/R_CS.
IPR004476. RNase_II/RNase_R.
IPR011805. RNase_R.
IPR013668. RNase_R_HTH_12.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF08461. HTH_12. 1 hit.
PF08206. OB_RNB. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00357. CSP. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 4 hits.
TIGRFAMsiTIGR00358. 3_prime_RNase. 1 hit.
TIGR02063. RNase_R. 1 hit.
PROSITEiPS01175. RIBONUCLEASE_II. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNR_SHIFL
AccessioniPrimary (citable) accession number: P30851
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 10, 2003
Last modified: November 2, 2016
This is version 120 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.