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Protein

Signal transduction histidine-protein kinase BaeS

Gene

baeS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system BaeS/BaeR which responds to envelope stress (PubMed:12354228, PubMed:21317898). Activates expression of periplasmic chaperone spy in response to spheroplast formation, indole and P pili protein PapG overexpression (PubMed:12354228). Activates BaeR by phosphorylation which then activates the mdtABCD (PubMed:12107134) and probably the CRISPR-Cas casABCDE-ygbT-ygbF operons (PubMed:21255106).4 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Stress response, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:BAES-MONOMER.
ECOL316407:JW2063-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transduction histidine-protein kinase BaeS (EC:2.7.13.3)
Gene namesi
Name:baeS1 Publication
Ordered Locus Names:b2078, JW2063
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11617. baeS.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Topological domaini33 – 167PeriplasmicSequence analysisAdd BLAST135
Transmembranei168 – 186HelicalSequence analysisAdd BLAST19
Topological domaini187 – 467CytoplasmicSequence analysisAdd BLAST281

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Loss of induction of the CRISPR-Cas casABCDE-ygbT-ygbF operon (PubMed:21255106).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi150R → W: Increased expression of periplasmic chaperone Spy. 1 Publication1
Mutagenesisi192P → L: Increased expression of periplasmic chaperone Spy. 1 Publication1
Mutagenesisi264E → K: Increased expression of periplasmic chaperone Spy. 1 Publication1
Mutagenesisi268D → N: Increased expression of periplasmic chaperone Spy. 1 Publication1
Mutagenesisi416R → C: Increased expression of periplasmic chaperone Spy, increased mRNA for mdtA and acrD, target genes of the BaeSR envelope stress response. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000746961 – 467Signal transduction histidine-protein kinase BaeSAdd BLAST467

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei250Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30847.
PRIDEiP30847.

Interactioni

Protein-protein interaction databases

DIPiDIP-9199N.
IntActiP30847. 3 interactors.
STRINGi511145.b2078.

Structurei

3D structure databases

ProteinModelPortaliP30847.
SMRiP30847.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 239HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini247 – 461Histidine kinasePROSITE-ProRule annotationAdd BLAST215

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EB3. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000223179.
InParanoidiP30847.
KOiK07642.
OMAiSNEQRIN.
PhylomeDBiP30847.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30847-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFWRPGITG KLFLAIFATC IVLLISMHWA VRISFERGFI DYIKHGNEQR
60 70 80 90 100
LQLLSDALGE QYAQHGNWRF LRNNDRFVFQ ILRSFEHDNS EDKPGPGMPP
110 120 130 140 150
HGWRTQFWVV DQNNKVLVGP RAPIPPDGTR RPILVNGAEV GAVIASPVER
160 170 180 190 200
LTRNTDINFD KQQRQTSWLI VALATLLAAL ATFLLARGLL APVKRLVDGT
210 220 230 240 250
HKLAAGDFTT RVTPTSEDEL GKLAQDFNQL ASTLEKNQQM RRDFMADISH
260 270 280 290 300
ELRTPLAVLR GELEAIQDGV RKFTPETVAS LQAEVGTLTK LVDDLHQLSM
310 320 330 340 350
SDEGALAYQK APVDLIPLLE VAGGAFRERF ASRGLKLQFS LPDSITVFGD
360 370 380 390 400
RDRLMQLFNN LLENSLRYTD SGGSLQISAG QRDKTVRLTF ADSAPGVSDD
410 420 430 440 450
QLQKLFERFY RTEGSRNRAS GGSGLGLAIC LNIVEAHNGR IIAAHSPFGG
460
VSITVELPLE RDLQREV
Length:467
Mass (Da):51,991
Last modified:November 1, 1997 - v2
Checksum:i024D76950012FFE0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti414 – 421GSRNRASG → VPATVPA in BAA03140 (PubMed:8282725).Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14054 Genomic DNA. Translation: BAA03140.1.
U00096 Genomic DNA. Translation: AAC75139.1.
AP009048 Genomic DNA. Translation: BAA15934.1.
PIRiE64974.
RefSeqiNP_416582.1. NC_000913.3.
WP_000675150.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75139; AAC75139; b2078.
BAA15934; BAA15934; BAA15934.
GeneIDi946611.
KEGGiecj:JW2063.
eco:b2078.
PATRICi32119489. VBIEscCol129921_2155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14054 Genomic DNA. Translation: BAA03140.1.
U00096 Genomic DNA. Translation: AAC75139.1.
AP009048 Genomic DNA. Translation: BAA15934.1.
PIRiE64974.
RefSeqiNP_416582.1. NC_000913.3.
WP_000675150.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP30847.
SMRiP30847.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9199N.
IntActiP30847. 3 interactors.
STRINGi511145.b2078.

Proteomic databases

PaxDbiP30847.
PRIDEiP30847.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75139; AAC75139; b2078.
BAA15934; BAA15934; BAA15934.
GeneIDi946611.
KEGGiecj:JW2063.
eco:b2078.
PATRICi32119489. VBIEscCol129921_2155.

Organism-specific databases

EchoBASEiEB1574.
EcoGeneiEG11617. baeS.

Phylogenomic databases

eggNOGiENOG4105EB3. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000223179.
InParanoidiP30847.
KOiK07642.
OMAiSNEQRIN.
PhylomeDBiP30847.

Enzyme and pathway databases

BioCyciEcoCyc:BAES-MONOMER.
ECOL316407:JW2063-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

PROiP30847.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAES_ECOLI
AccessioniPrimary (citable) accession number: P30847
Secondary accession number(s): P76401
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.