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Protein

Signal transduction histidine-protein kinase BaeS

Gene

baeS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system BaeS/BaeR which responds to envelope stress (PubMed:12354228, PubMed:21317898). Activates expression of periplasmic chaperone spy in response to spheroplast formation, indole and P pili protein PapG overexpression (PubMed:12354228). Activates BaeR by phosphorylation which then activates the mdtABCD (PubMed:12107134) and probably the CRISPR-Cas casABCDE-ygbT-ygbF operons (PubMed:21255106).4 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Stress response, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:BAES-MONOMER.
ECOL316407:JW2063-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transduction histidine-protein kinase BaeS (EC:2.7.13.3)
Gene namesi
Name:baeS1 Publication
Ordered Locus Names:b2078, JW2063
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11617. baeS.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence analysisAdd
BLAST
Transmembranei12 – 3221HelicalSequence analysisAdd
BLAST
Topological domaini33 – 167135PeriplasmicSequence analysisAdd
BLAST
Transmembranei168 – 18619HelicalSequence analysisAdd
BLAST
Topological domaini187 – 467281CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Loss of induction of the CRISPR-Cas casABCDE-ygbT-ygbF operon (PubMed:21255106).1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi150 – 1501R → W: Increased expression of periplasmic chaperone Spy. 1 Publication
Mutagenesisi192 – 1921P → L: Increased expression of periplasmic chaperone Spy. 1 Publication
Mutagenesisi264 – 2641E → K: Increased expression of periplasmic chaperone Spy. 1 Publication
Mutagenesisi268 – 2681D → N: Increased expression of periplasmic chaperone Spy. 1 Publication
Mutagenesisi416 – 4161R → C: Increased expression of periplasmic chaperone Spy, increased mRNA for mdtA and acrD, target genes of the BaeSR envelope stress response. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 467467Signal transduction histidine-protein kinase BaeSPRO_0000074696Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei250 – 2501Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30847.
PRIDEiP30847.

Interactioni

Protein-protein interaction databases

DIPiDIP-9199N.
IntActiP30847. 3 interactions.
STRINGi511145.b2078.

Structurei

3D structure databases

ProteinModelPortaliP30847.
SMRiP30847. Positions 192-461.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini187 – 23953HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini247 – 461215Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EB3. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000223179.
InParanoidiP30847.
KOiK07642.
OMAiSNEQRIN.
OrthoDBiEOG6G4VQG.
PhylomeDBiP30847.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30847-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFWRPGITG KLFLAIFATC IVLLISMHWA VRISFERGFI DYIKHGNEQR
60 70 80 90 100
LQLLSDALGE QYAQHGNWRF LRNNDRFVFQ ILRSFEHDNS EDKPGPGMPP
110 120 130 140 150
HGWRTQFWVV DQNNKVLVGP RAPIPPDGTR RPILVNGAEV GAVIASPVER
160 170 180 190 200
LTRNTDINFD KQQRQTSWLI VALATLLAAL ATFLLARGLL APVKRLVDGT
210 220 230 240 250
HKLAAGDFTT RVTPTSEDEL GKLAQDFNQL ASTLEKNQQM RRDFMADISH
260 270 280 290 300
ELRTPLAVLR GELEAIQDGV RKFTPETVAS LQAEVGTLTK LVDDLHQLSM
310 320 330 340 350
SDEGALAYQK APVDLIPLLE VAGGAFRERF ASRGLKLQFS LPDSITVFGD
360 370 380 390 400
RDRLMQLFNN LLENSLRYTD SGGSLQISAG QRDKTVRLTF ADSAPGVSDD
410 420 430 440 450
QLQKLFERFY RTEGSRNRAS GGSGLGLAIC LNIVEAHNGR IIAAHSPFGG
460
VSITVELPLE RDLQREV
Length:467
Mass (Da):51,991
Last modified:November 1, 1997 - v2
Checksum:i024D76950012FFE0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti414 – 4218GSRNRASG → VPATVPA in BAA03140 (PubMed:8282725).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14054 Genomic DNA. Translation: BAA03140.1.
U00096 Genomic DNA. Translation: AAC75139.1.
AP009048 Genomic DNA. Translation: BAA15934.1.
PIRiE64974.
RefSeqiNP_416582.1. NC_000913.3.
WP_000675150.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75139; AAC75139; b2078.
BAA15934; BAA15934; BAA15934.
GeneIDi946611.
KEGGiecj:JW2063.
eco:b2078.
PATRICi32119489. VBIEscCol129921_2155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14054 Genomic DNA. Translation: BAA03140.1.
U00096 Genomic DNA. Translation: AAC75139.1.
AP009048 Genomic DNA. Translation: BAA15934.1.
PIRiE64974.
RefSeqiNP_416582.1. NC_000913.3.
WP_000675150.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP30847.
SMRiP30847. Positions 192-461.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9199N.
IntActiP30847. 3 interactions.
STRINGi511145.b2078.

Proteomic databases

PaxDbiP30847.
PRIDEiP30847.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75139; AAC75139; b2078.
BAA15934; BAA15934; BAA15934.
GeneIDi946611.
KEGGiecj:JW2063.
eco:b2078.
PATRICi32119489. VBIEscCol129921_2155.

Organism-specific databases

EchoBASEiEB1574.
EcoGeneiEG11617. baeS.

Phylogenomic databases

eggNOGiENOG4105EB3. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000223179.
InParanoidiP30847.
KOiK07642.
OMAiSNEQRIN.
OrthoDBiEOG6G4VQG.
PhylomeDBiP30847.

Enzyme and pathway databases

BioCyciEcoCyc:BAES-MONOMER.
ECOL316407:JW2063-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

PROiP30847.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel members of the two-component signal transduction genes in Escherichia coli."
    Nagasawa S., Ishige K., Mizuno T.
    J. Biochem. 114:350-357(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The baeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate."
    Baranova N., Nikaido H.
    J. Bacteriol. 184:4168-4176(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN REGULATION OF THE MDTABCD OPERON.
    Strain: K12.
  6. "A third envelope stress signal transduction pathway in Escherichia coli."
    Raffa R.G., Raivio T.L.
    Mol. Microbiol. 45:1599-1611(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ENVELOPE STRESS RESPONSE.
    Strain: K12 / MC4100.
  7. "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli."
    Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.
    J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, AUTOPHOSPHORYLATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.
  9. "Envelope stress is a trigger of CRISPR RNA-mediated DNA silencing in Escherichia coli."
    Perez-Rodriguez R., Haitjema C., Huang Q., Nam K.H., Bernardis S., Ke A., DeLisa M.P.
    Mol. Microbiol. 79:584-599(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION OF THE CASABCDE-YGBT-YGBF OPERON, DISRUPTION PHENOTYPE.
    Strain: K12 / BW25113.
  10. Cited for: FUNCTION, MUTAGENESIS OF ARG-150; PRO-192; GLU-264; ASP-268 AND ARG-416.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiBAES_ECOLI
AccessioniPrimary (citable) accession number: P30847
Secondary accession number(s): P76401
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: July 6, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.