Reviewed, UniProtKB/Swiss-Prot P30847 (BAES_ECOLI)

Names and origin

Protein names

Recommended name:
    Signal transduction histidine-protein kinase baeS
    EC=2.7.13.3

Gene names

Name:	baeS
Ordered Locus Names:	b2078, JW2063

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of the two-component regulatory system baeS/baeR. May activate baeR by phosphorylation.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein. Ref.5
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Signal transduction histidine-protein kinase baeS		PRO_0000074696
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Potential
	Topological domain	33 – 167	135	Periplasmic Potential
	Transmembrane	168 – 186	19	Potential
	Topological domain	187 – 467	281	Cytoplasmic Potential
	Domain	187 – 239	53	HAMP
	Domain	247 – 461	215	Histidine kinase
Amino acid modifications
	Modified residue	250	1	Phosphohistidine; by autocatalysis By similarity
Experimental info
	Sequence conflict	414 – 421	8	GSRNRASG → VPATVPA in BAA03140. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P30847-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 024D76950012FFE0	FASTA	467	51,991
	10 20 30 40 50 60 MKFWRPGITG KLFLAIFATC IVLLISMHWA VRISFERGFI DYIKHGNEQR LQLLSDALGE 70 80 90 100 110 120 QYAQHGNWRF LRNNDRFVFQ ILRSFEHDNS EDKPGPGMPP HGWRTQFWVV DQNNKVLVGP 130 140 150 160 170 180 RAPIPPDGTR RPILVNGAEV GAVIASPVER LTRNTDINFD KQQRQTSWLI VALATLLAAL 190 200 210 220 230 240 ATFLLARGLL APVKRLVDGT HKLAAGDFTT RVTPTSEDEL GKLAQDFNQL ASTLEKNQQM 250 260 270 280 290 300 RRDFMADISH ELRTPLAVLR GELEAIQDGV RKFTPETVAS LQAEVGTLTK LVDDLHQLSM 310 320 330 340 350 360 SDEGALAYQK APVDLIPLLE VAGGAFRERF ASRGLKLQFS LPDSITVFGD RDRLMQLFNN 370 380 390 400 410 420 LLENSLRYTD SGGSLQISAG QRDKTVRLTF ADSAPGVSDD QLQKLFERFY RTEGSRNRAS 430 440 450 460 GGSGLGLAIC LNIVEAHNGR IIAAHSPFGG VSITVELPLE RDLQREV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel members of the two-component signal transduction genes in Escherichia coli." Nagasawa S., Ishige K., Mizuno T. J. Biochem. 114:350-357(1993) [PubMed: 8282725] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D14054 Genomic DNA. Translation: BAA03140.1. U00096 Genomic DNA. Translation: AAC75139.1. AP009048 Genomic DNA. Translation: BAA15934.1.
PIR	E64974.
RefSeq	AP_002678.1. NP_416582.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9199N.
Genome annotation databases
GeneID	946611.
GenomeReviews	Gene locus JW2063 in contig AP009048_GR. Gene locus b2078 in contig U00096_GR.
KEGG	ecj:JW2063. eco:b2078.
Organism-specific databases
EchoBASE	EB1574.
EcoGene	EG11617. baeS.
CMR	Search...
Phylogenomic databases
HOGENOM	P30847.
OMA	P30847. PSERINE.
Enzyme and pathway databases
BioCyc	EcoCyc:BAES-MON.
BRENDA	2.7.13.3. 246.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BAES_ECOLI

Accession

Primary (citable) accession number: P30847
Secondary accession number(s): P76401

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)