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Protein

Phosphoethanolamine transferase EptA

Gene

eptA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin (By similarity).By similarity

GO - Molecular functioni

  • phosphotransferase activity, phosphate group as acceptor Source: EcoCyc
  • sulfuric ester hydrolase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAntibiotic resistance, Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11613-MONOMER
MetaCyc:EG11613-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoethanolamine transferase EptA (EC:2.7.-.-)
Alternative name(s):
Polymyxin resistance protein PmrC
Gene namesi
Name:eptA
Synonyms:pmrC, yjdB
Ordered Locus Names:b4114, JW5730
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11613 eptA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Topological domaini31 – 47PeriplasmicSequence analysisAdd BLAST17
Transmembranei48 – 68HelicalSequence analysisAdd BLAST21
Topological domaini69 – 79CytoplasmicSequence analysisAdd BLAST11
Transmembranei80 – 100HelicalSequence analysisAdd BLAST21
Topological domaini101 – 123PeriplasmicSequence analysisAdd BLAST23
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Topological domaini145 – 154CytoplasmicSequence analysis10
Transmembranei155 – 175HelicalSequence analysisAdd BLAST21
Topological domaini176 – 547PeriplasmicSequence analysisAdd BLAST372

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002091511 – 547Phosphoethanolamine transferase EptAAdd BLAST547

Proteomic databases

PaxDbiP30845
PRIDEiP30845

Expressioni

Inductioni

The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions.By similarity

Interactioni

Subunit structurei

Has been isolated as a 91 kDa complex containing ZipA-EptA and an unidentified 24 kDa protein.1 Publication

Protein-protein interaction databases

BioGridi4263082, 125 interactors
DIPiDIP-12561N
IntActiP30845, 2 interactors
STRINGi316385.ECDH10B_4306

Structurei

3D structure databases

ProteinModelPortaliP30845
SMRiP30845
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DIJ Bacteria
COG2194 LUCA
HOGENOMiHOG000275171
InParanoidiP30845
KOiK03760
OMAiLMNAKNW
PhylomeDBiP30845

Family and domain databases

Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR017850 Alkaline_phosphatase_core_sf
IPR012549 EptA-like_N
IPR000917 Sulfatase_N
PfamiView protein in Pfam
PF08019 DUF1705, 1 hit
PF00884 Sulfatase, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit

Sequencei

Sequence statusi: Complete.

P30845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKRLLKRPS LNLLAWLLLA AFYISICLNI AFFKQVLQAL PLDSLHNVLV
60 70 80 90 100
FLSMPVVAFS VINIVLTLSS FLWLNRPLAC LFILVGAAAQ YFIMTYGIVI
110 120 130 140 150
DRSMIANIID TTPAESYALM TPQMLLTLGF SGVLAALIAC WIKIKPATSR
160 170 180 190 200
LRSVLFRGAN ILVSVLLILL VAALFYKDYA SLFRNNKELV KSLSPSNSIV
210 220 230 240 250
ASWSWYSHQR LANLPLVRIG EDAHRNPLMQ NEKRKNLTIL IVGETSRAEN
260 270 280 290 300
FSLNGYPRET NPRLAKDNVV YFPNTASCGT ATAVSVPCMF SDMPREHYKE
310 320 330 340 350
ELAQHQEGVL DIIQRAGINV LWNDNDGGCK GACDRVPHQN VTALNLPDQC
360 370 380 390 400
INGECYDEVL FHGLEEYINN LQGDGVIVLH TIGSHGPTYY NRYPPQFRKF
410 420 430 440 450
TPTCDTNEIQ TCTKEQLVNT YDNTLVYVDY IVDKAINLLK EHQDKFTTSL
460 470 480 490 500
VYLSDHGESL GENGIYLHGL PYAIAPDSQK QVPMLLWLSE DYQKRYQVDQ
510 520 530 540
NCLQKQAQTQ HYSQDNLFST LLGLTGVETK YYQAADDILQ TCRRVSE
Length:547
Mass (Da):61,667
Last modified:February 1, 1995 - v2
Checksum:iAA8CE79DDC2F8D10
GO

Sequence cautioni

The sequence AAA97013 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97013.1 Different initiation.
U00096 Genomic DNA Translation: AAC77075.2
AP009048 Genomic DNA Translation: BAE78116.1
D14055 Genomic DNA Translation: BAA03142.1
RefSeqiNP_418538.2, NC_000913.3
WP_000919792.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77075; AAC77075; b4114
BAE78116; BAE78116; BAE78116
GeneIDi948629
KEGGiecj:JW5730
eco:b4114
PATRICifig|1411691.4.peg.2586

Similar proteinsi

Entry informationi

Entry nameiEPTA_ECOLI
AccessioniPrimary (citable) accession number: P30845
Secondary accession number(s): P76793, Q2M6J0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health