Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoethanolamine transferase EptA

Gene

eptA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin (By similarity).By similarity

GO - Molecular functioni

  • phosphotransferase activity, phosphate group as acceptor Source: EcoCyc
  • sulfuric ester hydrolase activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Antibiotic resistance, Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11613-MONOMER.
ECOL316407:JW5730-MONOMER.
MetaCyc:EG11613-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoethanolamine transferase EptA (EC:2.7.-.-)
Alternative name(s):
Polymyxin resistance protein PmrC
Gene namesi
Name:eptA
Synonyms:pmrC, yjdB
Ordered Locus Names:b4114, JW5730
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11613. eptA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence analysis
Transmembranei10 – 3021HelicalSequence analysisAdd
BLAST
Topological domaini31 – 4717PeriplasmicSequence analysisAdd
BLAST
Transmembranei48 – 6821HelicalSequence analysisAdd
BLAST
Topological domaini69 – 7911CytoplasmicSequence analysisAdd
BLAST
Transmembranei80 – 10021HelicalSequence analysisAdd
BLAST
Topological domaini101 – 12323PeriplasmicSequence analysisAdd
BLAST
Transmembranei124 – 14421HelicalSequence analysisAdd
BLAST
Topological domaini145 – 15410CytoplasmicSequence analysis
Transmembranei155 – 17521HelicalSequence analysisAdd
BLAST
Topological domaini176 – 547372PeriplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 547547Phosphoethanolamine transferase EptAPRO_0000209151Add
BLAST

Proteomic databases

PaxDbiP30845.
PRIDEiP30845.

Expressioni

Inductioni

The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions.By similarity

Interactioni

Subunit structurei

Has been isolated as a 91 kDa complex containing ZipA-EptA and an unidentified 24 kDa protein.1 Publication

Protein-protein interaction databases

BioGridi4263082. 117 interactions.
DIPiDIP-12561N.
IntActiP30845. 2 interactions.
MINTiMINT-1218890.
STRINGi511145.b4114.

Structurei

3D structure databases

ProteinModelPortaliP30845.
SMRiP30845. Positions 235-544.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DIJ. Bacteria.
COG2194. LUCA.
HOGENOMiHOG000275171.
InParanoidiP30845.
KOiK03760.
OMAiFKNNAYE.
OrthoDBiEOG62ZHS6.
PhylomeDBiP30845.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR012549. DUF1705.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF08019. DUF1705. 1 hit.
PF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

P30845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKRLLKRPS LNLLAWLLLA AFYISICLNI AFFKQVLQAL PLDSLHNVLV
60 70 80 90 100
FLSMPVVAFS VINIVLTLSS FLWLNRPLAC LFILVGAAAQ YFIMTYGIVI
110 120 130 140 150
DRSMIANIID TTPAESYALM TPQMLLTLGF SGVLAALIAC WIKIKPATSR
160 170 180 190 200
LRSVLFRGAN ILVSVLLILL VAALFYKDYA SLFRNNKELV KSLSPSNSIV
210 220 230 240 250
ASWSWYSHQR LANLPLVRIG EDAHRNPLMQ NEKRKNLTIL IVGETSRAEN
260 270 280 290 300
FSLNGYPRET NPRLAKDNVV YFPNTASCGT ATAVSVPCMF SDMPREHYKE
310 320 330 340 350
ELAQHQEGVL DIIQRAGINV LWNDNDGGCK GACDRVPHQN VTALNLPDQC
360 370 380 390 400
INGECYDEVL FHGLEEYINN LQGDGVIVLH TIGSHGPTYY NRYPPQFRKF
410 420 430 440 450
TPTCDTNEIQ TCTKEQLVNT YDNTLVYVDY IVDKAINLLK EHQDKFTTSL
460 470 480 490 500
VYLSDHGESL GENGIYLHGL PYAIAPDSQK QVPMLLWLSE DYQKRYQVDQ
510 520 530 540
NCLQKQAQTQ HYSQDNLFST LLGLTGVETK YYQAADDILQ TCRRVSE
Length:547
Mass (Da):61,667
Last modified:February 1, 1995 - v2
Checksum:iAA8CE79DDC2F8D10
GO

Sequence cautioni

The sequence AAA97013.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97013.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77075.2.
AP009048 Genomic DNA. Translation: BAE78116.1.
D14055 Genomic DNA. Translation: BAA03142.1.
RefSeqiNP_418538.2. NC_000913.3.
WP_000919792.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77075; AAC77075; b4114.
BAE78116; BAE78116; BAE78116.
GeneIDi948629.
KEGGiecj:JW5730.
eco:b4114.
PATRICi32123793. VBIEscCol129921_4245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97013.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77075.2.
AP009048 Genomic DNA. Translation: BAE78116.1.
D14055 Genomic DNA. Translation: BAA03142.1.
RefSeqiNP_418538.2. NC_000913.3.
WP_000919792.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP30845.
SMRiP30845. Positions 235-544.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263082. 117 interactions.
DIPiDIP-12561N.
IntActiP30845. 2 interactions.
MINTiMINT-1218890.
STRINGi511145.b4114.

Proteomic databases

PaxDbiP30845.
PRIDEiP30845.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77075; AAC77075; b4114.
BAE78116; BAE78116; BAE78116.
GeneIDi948629.
KEGGiecj:JW5730.
eco:b4114.
PATRICi32123793. VBIEscCol129921_4245.

Organism-specific databases

EchoBASEiEB1570.
EcoGeneiEG11613. eptA.

Phylogenomic databases

eggNOGiENOG4105DIJ. Bacteria.
COG2194. LUCA.
HOGENOMiHOG000275171.
InParanoidiP30845.
KOiK03760.
OMAiFKNNAYE.
OrthoDBiEOG62ZHS6.
PhylomeDBiP30845.

Enzyme and pathway databases

BioCyciEcoCyc:EG11613-MONOMER.
ECOL316407:JW5730-MONOMER.
MetaCyc:EG11613-MONOMER.

Miscellaneous databases

PROiP30845.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR012549. DUF1705.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF08019. DUF1705. 1 hit.
PF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Novel members of the two-component signal transduction genes in Escherichia coli."
    Nagasawa S., Ishige K., Mizuno T.
    J. Biochem. 114:350-357(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 311-547.
    Strain: K12.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: BL21-DE3.

Entry informationi

Entry nameiEPTA_ECOLI
AccessioniPrimary (citable) accession number: P30845
Secondary accession number(s): P76793, Q2M6J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.