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P30844 (BASS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sensor protein BasS

EC=2.7.13.3
Gene names
Name:basS
Synonyms:pmrB
Ordered Locus Names:b4112, JW4073
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Member of the two-component regulatory system BasS/BasR Autophosphorylates and activates BasR by phosphorylation. Ref.5

Catalytic activity

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Induction

The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions By similarity.

Post-translational modification

Autophosphorylated. Ref.5

Sequence similarities

Contains 1 HAMP domain.

Contains 1 histidine kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 363363Sensor protein BasS
PRO_0000074701

Regions

Topological domain1 – 1313Cytoplasmic Potential
Transmembrane14 – 3421Helical; Potential
Topological domain35 – 6430Periplasmic Potential
Transmembrane65 – 8824Helical; Potential
Topological domain89 – 363275Cytoplasmic Potential
Domain89 – 14153HAMP
Domain149 – 357209Histidine kinase

Amino acid modifications

Modified residue1521Phosphohistidine; by autocatalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
P30844 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: A390AAB9EB425750

FASTA36341,029
        10         20         30         40         50         60 
MHFLRRPISL RQRLILTIGA ILLVFELISV FWLWHESTEQ IQLFEQALRD NRNNDRHIMR 

        70         80         90        100        110        120 
EIREAVASLI VPGVFMVSLT LFICYQAVRR ITRPLAELQK ELEARTADNL TPIAIHSATL 

       130        140        150        160        170        180 
EIEAVVSALN DLVSRLTSTL DNERLFTADV AHELRTPLAG VRLHLELLAK THHIDVAPLV 

       190        200        210        220        230        240 
ARLDQMMESV SQLLQLARAG QSFSSGNYQH VKLLEDVILP SYDELSTMLD QRQQTLLLPE 

       250        260        270        280        290        300 
SAADITVQGD ATLLRMLLRN LVENAHRYSP QGSNIMIKLQ EDDGAVMAVE DEGPGIDESK 

       310        320        330        340        350        360 
CGELSKAFVR MDSRYGGIGL GLSIVSRITQ LHHGQFFLQN RQETSGTRAW VRLKKDQYVA 


NQI 

« Hide

References

« Hide 'large scale' references
[1]"Novel members of the two-component signal transduction genes in Escherichia coli."
Nagasawa S., Ishige K., Mizuno T.
J. Biochem. 114:350-357(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli."
Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.
J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, AUTOPHOSPHORYLATION.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D14055 Genomic DNA. Translation: BAA03144.1.
U14003 Genomic DNA. Translation: AAA97011.1.
U00096 Genomic DNA. Translation: AAC77073.1.
AP009048 Genomic DNA. Translation: BAE78114.1.
PIRJX0285.
RefSeqNP_418536.1. NC_000913.2.
YP_492255.1. NC_007779.1.

3D structure databases

ProteinModelPortalP30844.
SMRP30844. Positions 128-351.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-9202N.
IntActP30844. 4 interactions.
MINTMINT-1257146.
STRING511145.b4112.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77073; AAC77073; b4112.
BAE78114; BAE78114; BAE78114.
GeneID12934090.
948632.
KEGGecj:Y75_p3999.
eco:b4112.
PATRIC32123789. VBIEscCol129921_4243.

Organism-specific databases

EchoBASEEB1571.
EcoGeneEG11614. basS.

Phylogenomic databases

eggNOGCOG0642.
HOGENOMHOG000127051.
KOK07643.
OMANESPNIE.
ProtClustDBPRK10755.

Enzyme and pathway databases

BioCycEcoCyc:BASS-MONOMER.
ECOL316407:JW4073-MONOMER.
BRENDA2.7.13.3. 2026.

Gene expression databases

GenevestigatorP30844.

Family and domain databases

Gene3D1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProIPR003660. HAMP_linker_domain.
IPR003594. HATPase_ATP-bd.
IPR004358. Sig_transdc_His_kin-like_C.
IPR003661. Sig_transdc_His_kin_sub1_dim/P.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
[Graphical view]
PfamPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSPR00344. BCTRLSENSOR.
SMARTSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMSSF55874. ATP_bd_ATPase. 1 hit.
SSF47384. His_kin_homodim. 1 hit.
PROSITEPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBASS_ECOLI
AccessionPrimary (citable) accession number: P30844
Secondary accession number(s): Q2M6J2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 1, 2013
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families