P30844 (BASS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sensor protein BasS
EC=2.7.13.3

Gene names

Name:	basS
Synonyms:	pmrB
Ordered Locus Names:	b4112, JW4073

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system BasS/BasR Autophosphorylates and activates BasR by phosphorylation. Ref.5
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Induction	The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions By similarity.
Post-translational modification	Autophosphorylated. Ref.5
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-histidine phosphorylation Inferred from direct assay Ref.5. Source: EcoCyc response to iron(III) ion Inferred from genetic interaction PubMed 12139617. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.1. Source: EcoCyc
Molecular_function	ATP binding Inferred from direct assay Ref.5. Source: EcoCyc phosphorelay sensor kinase activity Inferred from mutant phenotype Ref.1. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Sensor protein BasS		PRO_0000074701
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 34	21	Helical; Potential
	Topological domain	35 – 64	30	Periplasmic Potential
	Transmembrane	65 – 88	24	Helical; Potential
	Topological domain	89 – 363	275	Cytoplasmic Potential
	Domain	89 – 141	53	HAMP
	Domain	149 – 357	209	Histidine kinase
Amino acid modifications
	Modified residue	152	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30844 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: A390AAB9EB425750	FASTA	363	41,029
	10 20 30 40 50 60 MHFLRRPISL RQRLILTIGA ILLVFELISV FWLWHESTEQ IQLFEQALRD NRNNDRHIMR 70 80 90 100 110 120 EIREAVASLI VPGVFMVSLT LFICYQAVRR ITRPLAELQK ELEARTADNL TPIAIHSATL 130 140 150 160 170 180 EIEAVVSALN DLVSRLTSTL DNERLFTADV AHELRTPLAG VRLHLELLAK THHIDVAPLV 190 200 210 220 230 240 ARLDQMMESV SQLLQLARAG QSFSSGNYQH VKLLEDVILP SYDELSTMLD QRQQTLLLPE 250 260 270 280 290 300 SAADITVQGD ATLLRMLLRN LVENAHRYSP QGSNIMIKLQ EDDGAVMAVE DEGPGIDESK 310 320 330 340 350 360 CGELSKAFVR MDSRYGGIGL GLSIVSRITQ LHHGQFFLQN RQETSGTRAW VRLKKDQYVA NQI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel members of the two-component signal transduction genes in Escherichia coli." Nagasawa S., Ishige K., Mizuno T. J. Biochem. 114:350-357(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A. J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, AUTOPHOSPHORYLATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14055 Genomic DNA. Translation: BAA03144.1. U14003 Genomic DNA. Translation: AAA97011.1. U00096 Genomic DNA. Translation: AAC77073.1. AP009048 Genomic DNA. Translation: BAE78114.1.
PIR	JX0285.
RefSeq	NP_418536.1. NC_000913.2. YP_492255.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P30844.
SMR	P30844. Positions 128-351.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9202N.
IntAct	P30844. 4 interactions.
MINT	MINT-1257146.
STRING	511145.b4112.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77073; AAC77073; b4112. BAE78114; BAE78114; BAE78114.
GeneID	12934090. 948632.
KEGG	ecj:Y75_p3999. eco:b4112.
PATRIC	32123789. VBIEscCol129921_4243.
Organism-specific databases
EchoBASE	EB1571.
EcoGene	EG11614. basS.
Phylogenomic databases
eggNOG	COG0642.
HOGENOM	HOG000127051.
KO	K07643.
OMA	NESPNIE.
ProtClustDB	PRK10755.
Enzyme and pathway databases
BioCyc	EcoCyc:BASS-MONOMER. ECOL316407:JW4073-MONOMER.
BRENDA	2.7.13.3. 2026.
Gene expression databases
Genevestigator	P30844.
Family and domain databases
Gene3D	1.10.287.130. 1 hit. 3.30.565.10. 1 hit.
InterPro	IPR003660. HAMP_linker_domain. IPR003594. HATPase_ATP-bd. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF47384. His_kin_homodim. 1 hit.
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BASS_ECOLI

Accession

Primary (citable) accession number: P30844
Secondary accession number(s): Q2M6J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 125 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program