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Protein

Sensor protein BasS

Gene

basS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system BasS/BasR Autophosphorylates and activates BasR by phosphorylation.1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc
  • protein histidine kinase activity Source: EcoCyc
  • transferase activity, transferring phosphorus-containing groups Source: EcoCyc

GO - Biological processi

  • peptidyl-histidine phosphorylation Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • response to iron(III) ion Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:BASS-MONOMER.
ECOL316407:JW4073-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein BasS (EC:2.7.13.3)
Gene namesi
Name:basS
Synonyms:pmrB
Ordered Locus Names:b4112, JW4073
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11614. basS.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicSequence analysisAdd
BLAST
Transmembranei14 – 3421HelicalSequence analysisAdd
BLAST
Topological domaini35 – 6430PeriplasmicSequence analysisAdd
BLAST
Transmembranei65 – 8824HelicalSequence analysisAdd
BLAST
Topological domaini89 – 363275CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Sensor protein BasSPRO_0000074701Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei152 – 1521Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30844.

Expressioni

Inductioni

The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions.By similarity

Interactioni

Protein-protein interaction databases

BioGridi4263084. 3 interactions.
DIPiDIP-9202N.
IntActiP30844. 4 interactions.
MINTiMINT-1257146.
STRINGi511145.b4112.

Structurei

3D structure databases

ProteinModelPortaliP30844.
SMRiP30844. Positions 91-351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini89 – 14153HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini149 – 357209Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105ECM. Bacteria.
ENOG410XPUQ. LUCA.
HOGENOMiHOG000127051.
InParanoidiP30844.
KOiK07643.
OMAiELISIFW.
OrthoDBiEOG6G4VQG.
PhylomeDBiP30844.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30844-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFLRRPISL RQRLILTIGA ILLVFELISV FWLWHESTEQ IQLFEQALRD
60 70 80 90 100
NRNNDRHIMR EIREAVASLI VPGVFMVSLT LFICYQAVRR ITRPLAELQK
110 120 130 140 150
ELEARTADNL TPIAIHSATL EIEAVVSALN DLVSRLTSTL DNERLFTADV
160 170 180 190 200
AHELRTPLAG VRLHLELLAK THHIDVAPLV ARLDQMMESV SQLLQLARAG
210 220 230 240 250
QSFSSGNYQH VKLLEDVILP SYDELSTMLD QRQQTLLLPE SAADITVQGD
260 270 280 290 300
ATLLRMLLRN LVENAHRYSP QGSNIMIKLQ EDDGAVMAVE DEGPGIDESK
310 320 330 340 350
CGELSKAFVR MDSRYGGIGL GLSIVSRITQ LHHGQFFLQN RQETSGTRAW
360
VRLKKDQYVA NQI
Length:363
Mass (Da):41,029
Last modified:July 1, 1993 - v1
Checksum:iA390AAB9EB425750
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14055 Genomic DNA. Translation: BAA03144.1.
U14003 Genomic DNA. Translation: AAA97011.1.
U00096 Genomic DNA. Translation: AAC77073.1.
AP009048 Genomic DNA. Translation: BAE78114.1.
PIRiJX0285.
RefSeqiNP_418536.1. NC_000913.3.
WP_001300761.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77073; AAC77073; b4112.
BAE78114; BAE78114; BAE78114.
GeneIDi948632.
KEGGiecj:JW4073.
eco:b4112.
PATRICi32123789. VBIEscCol129921_4243.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14055 Genomic DNA. Translation: BAA03144.1.
U14003 Genomic DNA. Translation: AAA97011.1.
U00096 Genomic DNA. Translation: AAC77073.1.
AP009048 Genomic DNA. Translation: BAE78114.1.
PIRiJX0285.
RefSeqiNP_418536.1. NC_000913.3.
WP_001300761.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP30844.
SMRiP30844. Positions 91-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263084. 3 interactions.
DIPiDIP-9202N.
IntActiP30844. 4 interactions.
MINTiMINT-1257146.
STRINGi511145.b4112.

Proteomic databases

PaxDbiP30844.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77073; AAC77073; b4112.
BAE78114; BAE78114; BAE78114.
GeneIDi948632.
KEGGiecj:JW4073.
eco:b4112.
PATRICi32123789. VBIEscCol129921_4243.

Organism-specific databases

EchoBASEiEB1571.
EcoGeneiEG11614. basS.

Phylogenomic databases

eggNOGiENOG4105ECM. Bacteria.
ENOG410XPUQ. LUCA.
HOGENOMiHOG000127051.
InParanoidiP30844.
KOiK07643.
OMAiELISIFW.
OrthoDBiEOG6G4VQG.
PhylomeDBiP30844.

Enzyme and pathway databases

BioCyciEcoCyc:BASS-MONOMER.
ECOL316407:JW4073-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

PROiP30844.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel members of the two-component signal transduction genes in Escherichia coli."
    Nagasawa S., Ishige K., Mizuno T.
    J. Biochem. 114:350-357(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli."
    Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.
    J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, AUTOPHOSPHORYLATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiBASS_ECOLI
AccessioniPrimary (citable) accession number: P30844
Secondary accession number(s): Q2M6J2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 6, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.