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P30843

- BASR_ECOLI

UniProt

P30843 - BASR_ECOLI

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Protein

Transcriptional regulatory protein BasR

Gene

basR

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin (By similarity).By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. phosphorelay response regulator activity Source: EcoCyc

GO - Biological processi

  1. phosphorelay signal transduction system Source: EcoCyc
  2. regulation of transcription, DNA-templated Source: EcoCyc
  3. response to antibiotic Source: UniProtKB-KW
  4. response to iron(III) ion Source: EcoCyc
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Antibiotic resistance, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:BASR-MONOMER.
ECOL316407:JW4074-MONOMER.
RETL1328306-WGS:GSTH-5535-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein BasR
Gene namesi
Name:basR
Synonyms:pmrA
Ordered Locus Names:b4113, JW4074
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11615. basR.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Transcriptional regulatory protein BasRPRO_0000081023Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 5114-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by BasS.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30843.
PRIDEiP30843.

Expressioni

Inductioni

The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions.By similarity

Gene expression databases

GenevestigatoriP30843.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

DIPiDIP-9201N.
IntActiP30843. 5 interactions.
STRINGi511145.b4113.

Structurei

3D structure databases

ProteinModelPortaliP30843.
SMRiP30843. Positions 2-118, 121-219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 116116Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0745.
InParanoidiP30843.
KOiK07771.
OMAiACDCAGT.
OrthoDBiEOG6G4VQG.
PhylomeDBiP30843.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30843-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKILIVEDDT LLLQGLILAA QTEGYACDSV TTARMAEQSL EAGHYSLVVL
60 70 80 90 100
DLGLPDEDGL HFLARIRQKK YTLPVLILTA RDTLTDKIAG LDVGADDYLV
110 120 130 140 150
KPFALEELHA RIRALLRRHN NQGESELIVG NLTLNMGRRQ VWMGGEELIL
160 170 180 190 200
TPKEYALLSR LMLKAGSPVH REILYNDIYN WDNEPSTNTL EVHIHNLRDK
210 220
VGKARIRTVR GFGYMLVANE EN
Length:222
Mass (Da):25,031
Last modified:August 29, 2003 - v3
Checksum:i8588F9F23A1B8625
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291S → T(PubMed:8282725)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 291S → G in strain: ECOR 1, ECOR 10, ECOR 11, ECOR 12, ECOR 13, ECOR 14, ECOR 15, ECOR 16, ECOR 17, ECOR 18, ECOR 19, ECOR 2, ECOR 20, ECOR 21, ECOR 22, ECOR 24, ECOR 25, ECOR 26, ECOR 27, ECOR 3, ECOR 30, ECOR 33, ECOR 34, ECOR 35, ECOR 36, ECOR 37, ECOR 38, ECOR 4, ECOR 40, ECOR 41, ECOR 42, ECOR 43, ECOR 44, ECOR 45, ECOR 46, ECOR 47, ECOR 48, ECOR 5, ECOR 50, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 6, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64, ECOR 65, ECOR 66, ECOR 67, ECOR 68, ECOR 69, ECOR 7, ECOR 70, ECOR 71, ECOR 72, ECOR 8 and ECOR 9.
Natural varianti31 – 311T → S in strain: ECOR 35, ECOR 36, ECOR 38, ECOR 40, ECOR 41, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63 and ECOR 64, ECOR 65 and ECOR 66.
Natural varianti128 – 1281I → N in strain: ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63 and ECOR 64.
Natural varianti129 – 1291V → L in strain: ECOR 19.
Natural varianti144 – 1441G → S in strain: ECOR 44, ECOR 48, ECOR 50, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64 and ECOR 72.
Natural varianti207 – 2071R → P in strain: ECOR 27.
Natural varianti214 – 2141Y → F in strain: ECOR 27.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14055 Genomic DNA. Translation: BAA03143.1.
AY725270 Genomic DNA. Translation: AAV92717.1.
AY725271 Genomic DNA. Translation: AAV92718.1.
AY725272 Genomic DNA. Translation: AAV92719.1.
AY725273 Genomic DNA. Translation: AAV92720.1.
AY725274 Genomic DNA. Translation: AAV92721.1.
AY725275 Genomic DNA. Translation: AAV92722.1.
AY725276 Genomic DNA. Translation: AAV92723.1.
AY725277 Genomic DNA. Translation: AAV92724.1.
AY725278 Genomic DNA. Translation: AAV92725.1.
AY725279 Genomic DNA. Translation: AAV92726.1.
AY725280 Genomic DNA. Translation: AAV92727.1.
AY725281 Genomic DNA. Translation: AAV92728.1.
AY725282 Genomic DNA. Translation: AAV92729.1.
AY725283 Genomic DNA. Translation: AAV92730.1.
AY725284 Genomic DNA. Translation: AAV92731.1.
AY725285 Genomic DNA. Translation: AAV92732.1.
AY725286 Genomic DNA. Translation: AAV92733.1.
AY725287 Genomic DNA. Translation: AAV92734.1.
AY725288 Genomic DNA. Translation: AAV92735.1.
AY725289 Genomic DNA. Translation: AAV92736.1.
AY725290 Genomic DNA. Translation: AAV92737.1.
AY725291 Genomic DNA. Translation: AAV92738.1.
AY725292 Genomic DNA. Translation: AAV92739.1.
AY725293 Genomic DNA. Translation: AAV92740.1.
AY725294 Genomic DNA. Translation: AAV92741.1.
AY725295 Genomic DNA. Translation: AAV92742.1.
AY725296 Genomic DNA. Translation: AAV92743.1.
AY725297 Genomic DNA. Translation: AAV92744.1.
AY725298 Genomic DNA. Translation: AAV92745.1.
AY725299 Genomic DNA. Translation: AAV92746.1.
AY725300 Genomic DNA. Translation: AAV92747.1.
AY725301 Genomic DNA. Translation: AAV92748.1.
AY725302 Genomic DNA. Translation: AAV92749.1.
AY725303 Genomic DNA. Translation: AAV92750.1.
AY725304 Genomic DNA. Translation: AAV92751.1.
AY725305 Genomic DNA. Translation: AAV92752.1.
AY725306 Genomic DNA. Translation: AAV92753.1.
AY725307 Genomic DNA. Translation: AAV92754.1.
AY725308 Genomic DNA. Translation: AAV92755.1.
AY725309 Genomic DNA. Translation: AAV92756.1.
AY725310 Genomic DNA. Translation: AAV92757.1.
AY725311 Genomic DNA. Translation: AAV92758.1.
AY725312 Genomic DNA. Translation: AAV92759.1.
AY725313 Genomic DNA. Translation: AAV92760.1.
AY725314 Genomic DNA. Translation: AAV92761.1.
AY725315 Genomic DNA. Translation: AAV92762.1.
AY725316 Genomic DNA. Translation: AAV92763.1.
AY725317 Genomic DNA. Translation: AAV92764.1.
AY725318 Genomic DNA. Translation: AAV92765.1.
AY725319 Genomic DNA. Translation: AAV92766.1.
AY725320 Genomic DNA. Translation: AAV92767.1.
AY725321 Genomic DNA. Translation: AAV92768.1.
AY725322 Genomic DNA. Translation: AAV92769.1.
AY725323 Genomic DNA. Translation: AAV92770.1.
AY725324 Genomic DNA. Translation: AAV92771.1.
AY725325 Genomic DNA. Translation: AAV92772.1.
AY725326 Genomic DNA. Translation: AAV92773.1.
AY725327 Genomic DNA. Translation: AAV92774.1.
AY725328 Genomic DNA. Translation: AAV92775.1.
AY725329 Genomic DNA. Translation: AAV92776.1.
AY725330 Genomic DNA. Translation: AAV92777.1.
AY725331 Genomic DNA. Translation: AAV92778.1.
AY725332 Genomic DNA. Translation: AAV92779.1.
AY725333 Genomic DNA. Translation: AAV92780.1.
U14003 Genomic DNA. Translation: AAA97012.1.
U00096 Genomic DNA. Translation: AAC77074.1.
AP009048 Genomic DNA. Translation: BAE78115.1.
PIRiH65220.
RefSeqiNP_418537.1. NC_000913.3.
YP_492256.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77074; AAC77074; b4113.
BAE78115; BAE78115; BAE78115.
GeneIDi12934089.
948631.
KEGGiecj:Y75_p4000.
eco:b4113.
PATRICi32123791. VBIEscCol129921_4244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14055 Genomic DNA. Translation: BAA03143.1 .
AY725270 Genomic DNA. Translation: AAV92717.1 .
AY725271 Genomic DNA. Translation: AAV92718.1 .
AY725272 Genomic DNA. Translation: AAV92719.1 .
AY725273 Genomic DNA. Translation: AAV92720.1 .
AY725274 Genomic DNA. Translation: AAV92721.1 .
AY725275 Genomic DNA. Translation: AAV92722.1 .
AY725276 Genomic DNA. Translation: AAV92723.1 .
AY725277 Genomic DNA. Translation: AAV92724.1 .
AY725278 Genomic DNA. Translation: AAV92725.1 .
AY725279 Genomic DNA. Translation: AAV92726.1 .
AY725280 Genomic DNA. Translation: AAV92727.1 .
AY725281 Genomic DNA. Translation: AAV92728.1 .
AY725282 Genomic DNA. Translation: AAV92729.1 .
AY725283 Genomic DNA. Translation: AAV92730.1 .
AY725284 Genomic DNA. Translation: AAV92731.1 .
AY725285 Genomic DNA. Translation: AAV92732.1 .
AY725286 Genomic DNA. Translation: AAV92733.1 .
AY725287 Genomic DNA. Translation: AAV92734.1 .
AY725288 Genomic DNA. Translation: AAV92735.1 .
AY725289 Genomic DNA. Translation: AAV92736.1 .
AY725290 Genomic DNA. Translation: AAV92737.1 .
AY725291 Genomic DNA. Translation: AAV92738.1 .
AY725292 Genomic DNA. Translation: AAV92739.1 .
AY725293 Genomic DNA. Translation: AAV92740.1 .
AY725294 Genomic DNA. Translation: AAV92741.1 .
AY725295 Genomic DNA. Translation: AAV92742.1 .
AY725296 Genomic DNA. Translation: AAV92743.1 .
AY725297 Genomic DNA. Translation: AAV92744.1 .
AY725298 Genomic DNA. Translation: AAV92745.1 .
AY725299 Genomic DNA. Translation: AAV92746.1 .
AY725300 Genomic DNA. Translation: AAV92747.1 .
AY725301 Genomic DNA. Translation: AAV92748.1 .
AY725302 Genomic DNA. Translation: AAV92749.1 .
AY725303 Genomic DNA. Translation: AAV92750.1 .
AY725304 Genomic DNA. Translation: AAV92751.1 .
AY725305 Genomic DNA. Translation: AAV92752.1 .
AY725306 Genomic DNA. Translation: AAV92753.1 .
AY725307 Genomic DNA. Translation: AAV92754.1 .
AY725308 Genomic DNA. Translation: AAV92755.1 .
AY725309 Genomic DNA. Translation: AAV92756.1 .
AY725310 Genomic DNA. Translation: AAV92757.1 .
AY725311 Genomic DNA. Translation: AAV92758.1 .
AY725312 Genomic DNA. Translation: AAV92759.1 .
AY725313 Genomic DNA. Translation: AAV92760.1 .
AY725314 Genomic DNA. Translation: AAV92761.1 .
AY725315 Genomic DNA. Translation: AAV92762.1 .
AY725316 Genomic DNA. Translation: AAV92763.1 .
AY725317 Genomic DNA. Translation: AAV92764.1 .
AY725318 Genomic DNA. Translation: AAV92765.1 .
AY725319 Genomic DNA. Translation: AAV92766.1 .
AY725320 Genomic DNA. Translation: AAV92767.1 .
AY725321 Genomic DNA. Translation: AAV92768.1 .
AY725322 Genomic DNA. Translation: AAV92769.1 .
AY725323 Genomic DNA. Translation: AAV92770.1 .
AY725324 Genomic DNA. Translation: AAV92771.1 .
AY725325 Genomic DNA. Translation: AAV92772.1 .
AY725326 Genomic DNA. Translation: AAV92773.1 .
AY725327 Genomic DNA. Translation: AAV92774.1 .
AY725328 Genomic DNA. Translation: AAV92775.1 .
AY725329 Genomic DNA. Translation: AAV92776.1 .
AY725330 Genomic DNA. Translation: AAV92777.1 .
AY725331 Genomic DNA. Translation: AAV92778.1 .
AY725332 Genomic DNA. Translation: AAV92779.1 .
AY725333 Genomic DNA. Translation: AAV92780.1 .
U14003 Genomic DNA. Translation: AAA97012.1 .
U00096 Genomic DNA. Translation: AAC77074.1 .
AP009048 Genomic DNA. Translation: BAE78115.1 .
PIRi H65220.
RefSeqi NP_418537.1. NC_000913.3.
YP_492256.1. NC_007779.1.

3D structure databases

ProteinModelPortali P30843.
SMRi P30843. Positions 2-118, 121-219.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9201N.
IntActi P30843. 5 interactions.
STRINGi 511145.b4113.

Proteomic databases

PaxDbi P30843.
PRIDEi P30843.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC77074 ; AAC77074 ; b4113 .
BAE78115 ; BAE78115 ; BAE78115 .
GeneIDi 12934089.
948631.
KEGGi ecj:Y75_p4000.
eco:b4113.
PATRICi 32123791. VBIEscCol129921_4244.

Organism-specific databases

EchoBASEi EB1572.
EcoGenei EG11615. basR.

Phylogenomic databases

eggNOGi COG0745.
InParanoidi P30843.
KOi K07771.
OMAi ACDCAGT.
OrthoDBi EOG6G4VQG.
PhylomeDBi P30843.

Enzyme and pathway databases

BioCyci EcoCyc:BASR-MONOMER.
ECOL316407:JW4074-MONOMER.
RETL1328306-WGS:GSTH-5535-MONOMER.

Miscellaneous databases

PROi P30843.

Gene expression databases

Genevestigatori P30843.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view ]
SMARTi SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view ]
SUPFAMi SSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Novel members of the two-component signal transduction genes in Escherichia coli."
    Nagasawa S., Ishige K., Mizuno T.
    J. Biochem. 114:350-357(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Phenotypic differences between Salmonella and Escherichia coli resulting from the disparate regulation of homologous genes."
    Winfield M.D., Groisman E.A.
    Proc. Natl. Acad. Sci. U.S.A. 101:17162-17167(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REGULATION BY PMRD.
    Strain: ECOR 1, ECOR 10, ECOR 11, ECOR 12, ECOR 13, ECOR 14, ECOR 15, ECOR 16, ECOR 17, ECOR 18, ECOR 19, ECOR 2, ECOR 20, ECOR 21, ECOR 22, ECOR 24, ECOR 25, ECOR 26, ECOR 27, ECOR 3, ECOR 30, ECOR 33, ECOR 34, ECOR 35, ECOR 36, ECOR 37, ECOR 38, ECOR 4, ECOR 40, ECOR 41, ECOR 42, ECOR 43, ECOR 44, ECOR 45, ECOR 46, ECOR 47, ECOR 48, ECOR 5, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 6, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64, ECOR 65, ECOR 66, ECOR 67, ECOR 68, ECOR 69, ECOR 7, ECOR 70, ECOR 71, ECOR 72, ECOR 8, ECOR 9 and O2:HN / ECOR 50 / P97 / UPEC.
  3. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli."
    Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.
    J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiBASR_ECOLI
AccessioniPrimary (citable) accession number: P30843
Secondary accession number(s): Q2M6J1
, Q5MNC7, Q5MNC9, Q5MNE0, Q5MNE5, Q5MNF2, Q5MNH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: August 29, 2003
Last modified: October 29, 2014
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The activity of the BasR protein is in some natural isolates, but not in strain K12, controlled at a post-transcriptional level by the PmrD protein under low Mg2+ concentration conditions.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3