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P30843 (BASR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional regulatory protein BasR
Gene names
Name:basR
Synonyms:pmrA
Ordered Locus Names:b4113, JW4074
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length222 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm Potential.

Induction

The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions By similarity. Ref.2

Post-translational modification

Phosphorylated by BasS. Ref.6

Miscellaneous

The activity of the BasR protein is in some natural isolates, but not in strain K12, controlled at a post-transcriptional level by the PmrD protein under low Mg2+ concentration conditions.

Sequence similarities

Contains 1 response regulatory domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 222222Transcriptional regulatory protein BasR
PRO_0000081023

Regions

Domain1 – 116116Response regulatory

Amino acid modifications

Modified residue5114-aspartylphosphate By similarity

Natural variations

Natural variant291S → G in strain: ECOR 1, ECOR 10, ECOR 11, ECOR 12, ECOR 13, ECOR 14, ECOR 15, ECOR 16, ECOR 17, ECOR 18, ECOR 19, ECOR 2, ECOR 20, ECOR 21, ECOR 22, ECOR 24, ECOR 25, ECOR 26, ECOR 27, ECOR 3, ECOR 30, ECOR 33, ECOR 34, ECOR 35, ECOR 36, ECOR 37, ECOR 38, ECOR 4, ECOR 40, ECOR 41, ECOR 42, ECOR 43, ECOR 44, ECOR 45, ECOR 46, ECOR 47, ECOR 48, ECOR 5, ECOR 50, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 6, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64, ECOR 65, ECOR 66, ECOR 67, ECOR 68, ECOR 69, ECOR 7, ECOR 70, ECOR 71, ECOR 72, ECOR 8 and ECOR 9.
Natural variant311T → S in strain: ECOR 35, ECOR 36, ECOR 38, ECOR 40, ECOR 41, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63 and ECOR 64, ECOR 65 and ECOR 66.
Natural variant1281I → N in strain: ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63 and ECOR 64.
Natural variant1291V → L in strain: ECOR 19.
Natural variant1441G → S in strain: ECOR 44, ECOR 48, ECOR 50, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64 and ECOR 72.
Natural variant2071R → P in strain: ECOR 27.
Natural variant2141Y → F in strain: ECOR 27.

Experimental info

Sequence conflict291S → T Ref.1

Sequences

Sequence LengthMass (Da)Tools
P30843 [UniParc].

Last modified August 29, 2003. Version 3.
Checksum: 8588F9F23A1B8625

FASTA22225,031
        10         20         30         40         50         60 
MKILIVEDDT LLLQGLILAA QTEGYACDSV TTARMAEQSL EAGHYSLVVL DLGLPDEDGL 

        70         80         90        100        110        120 
HFLARIRQKK YTLPVLILTA RDTLTDKIAG LDVGADDYLV KPFALEELHA RIRALLRRHN 

       130        140        150        160        170        180 
NQGESELIVG NLTLNMGRRQ VWMGGEELIL TPKEYALLSR LMLKAGSPVH REILYNDIYN 

       190        200        210        220 
WDNEPSTNTL EVHIHNLRDK VGKARIRTVR GFGYMLVANE EN 

« Hide

References

« Hide 'large scale' references
[1]"Novel members of the two-component signal transduction genes in Escherichia coli."
Nagasawa S., Ishige K., Mizuno T.
J. Biochem. 114:350-357(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Phenotypic differences between Salmonella and Escherichia coli resulting from the disparate regulation of homologous genes."
Winfield M.D., Groisman E.A.
Proc. Natl. Acad. Sci. U.S.A. 101:17162-17167(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REGULATION BY PMRD.
Strain: ECOR 1, ECOR 10, ECOR 11, ECOR 12, ECOR 13, ECOR 14, ECOR 15, ECOR 16, ECOR 17, ECOR 18, ECOR 19, ECOR 2, ECOR 20, ECOR 21, ECOR 22, ECOR 24, ECOR 25, ECOR 26, ECOR 27, ECOR 3, ECOR 30, ECOR 33, ECOR 34, ECOR 35, ECOR 36, ECOR 37, ECOR 38, ECOR 4, ECOR 40, ECOR 41, ECOR 42, ECOR 43, ECOR 44, ECOR 45, ECOR 46, ECOR 47, ECOR 48, ECOR 5, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 6, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64, ECOR 65, ECOR 66, ECOR 67, ECOR 68, ECOR 69, ECOR 7, ECOR 70, ECOR 71, ECOR 72, ECOR 8, ECOR 9 and O2:HN / ECOR 50 / P97 / UPEC.
[3]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli."
Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.
J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D14055 Genomic DNA. Translation: BAA03143.1.
AY725270 Genomic DNA. Translation: AAV92717.1.
AY725271 Genomic DNA. Translation: AAV92718.1.
AY725272 Genomic DNA. Translation: AAV92719.1.
AY725273 Genomic DNA. Translation: AAV92720.1.
AY725274 Genomic DNA. Translation: AAV92721.1.
AY725275 Genomic DNA. Translation: AAV92722.1.
AY725276 Genomic DNA. Translation: AAV92723.1.
AY725277 Genomic DNA. Translation: AAV92724.1.
AY725278 Genomic DNA. Translation: AAV92725.1.
AY725279 Genomic DNA. Translation: AAV92726.1.
AY725280 Genomic DNA. Translation: AAV92727.1.
AY725281 Genomic DNA. Translation: AAV92728.1.
AY725282 Genomic DNA. Translation: AAV92729.1.
AY725283 Genomic DNA. Translation: AAV92730.1.
AY725284 Genomic DNA. Translation: AAV92731.1.
AY725285 Genomic DNA. Translation: AAV92732.1.
AY725286 Genomic DNA. Translation: AAV92733.1.
AY725287 Genomic DNA. Translation: AAV92734.1.
AY725288 Genomic DNA. Translation: AAV92735.1.
AY725289 Genomic DNA. Translation: AAV92736.1.
AY725290 Genomic DNA. Translation: AAV92737.1.
AY725291 Genomic DNA. Translation: AAV92738.1.
AY725292 Genomic DNA. Translation: AAV92739.1.
AY725293 Genomic DNA. Translation: AAV92740.1.
AY725294 Genomic DNA. Translation: AAV92741.1.
AY725295 Genomic DNA. Translation: AAV92742.1.
AY725296 Genomic DNA. Translation: AAV92743.1.
AY725297 Genomic DNA. Translation: AAV92744.1.
AY725298 Genomic DNA. Translation: AAV92745.1.
AY725299 Genomic DNA. Translation: AAV92746.1.
AY725300 Genomic DNA. Translation: AAV92747.1.
AY725301 Genomic DNA. Translation: AAV92748.1.
AY725302 Genomic DNA. Translation: AAV92749.1.
AY725303 Genomic DNA. Translation: AAV92750.1.
AY725304 Genomic DNA. Translation: AAV92751.1.
AY725305 Genomic DNA. Translation: AAV92752.1.
AY725306 Genomic DNA. Translation: AAV92753.1.
AY725307 Genomic DNA. Translation: AAV92754.1.
AY725308 Genomic DNA. Translation: AAV92755.1.
AY725309 Genomic DNA. Translation: AAV92756.1.
AY725310 Genomic DNA. Translation: AAV92757.1.
AY725311 Genomic DNA. Translation: AAV92758.1.
AY725312 Genomic DNA. Translation: AAV92759.1.
AY725313 Genomic DNA. Translation: AAV92760.1.
AY725314 Genomic DNA. Translation: AAV92761.1.
AY725315 Genomic DNA. Translation: AAV92762.1.
AY725316 Genomic DNA. Translation: AAV92763.1.
AY725317 Genomic DNA. Translation: AAV92764.1.
AY725318 Genomic DNA. Translation: AAV92765.1.
AY725319 Genomic DNA. Translation: AAV92766.1.
AY725320 Genomic DNA. Translation: AAV92767.1.
AY725321 Genomic DNA. Translation: AAV92768.1.
AY725322 Genomic DNA. Translation: AAV92769.1.
AY725323 Genomic DNA. Translation: AAV92770.1.
AY725324 Genomic DNA. Translation: AAV92771.1.
AY725325 Genomic DNA. Translation: AAV92772.1.
AY725326 Genomic DNA. Translation: AAV92773.1.
AY725327 Genomic DNA. Translation: AAV92774.1.
AY725328 Genomic DNA. Translation: AAV92775.1.
AY725329 Genomic DNA. Translation: AAV92776.1.
AY725330 Genomic DNA. Translation: AAV92777.1.
AY725331 Genomic DNA. Translation: AAV92778.1.
AY725332 Genomic DNA. Translation: AAV92779.1.
AY725333 Genomic DNA. Translation: AAV92780.1.
U14003 Genomic DNA. Translation: AAA97012.1.
U00096 Genomic DNA. Translation: AAC77074.1.
AP009048 Genomic DNA. Translation: BAE78115.1.
PIRH65220.
RefSeqNP_418537.1. NC_000913.3.
YP_492256.1. NC_007779.1.

3D structure databases

ProteinModelPortalP30843.
SMRP30843. Positions 2-118, 121-219.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9201N.
IntActP30843. 5 interactions.
STRING511145.b4113.

Proteomic databases

PaxDbP30843.
PRIDEP30843.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77074; AAC77074; b4113.
BAE78115; BAE78115; BAE78115.
GeneID12934089.
948631.
KEGGecj:Y75_p4000.
eco:b4113.
PATRIC32123791. VBIEscCol129921_4244.

Organism-specific databases

EchoBASEEB1572.
EcoGeneEG11615. basR.

Phylogenomic databases

eggNOGCOG0745.
KOK07771.
OMAACDCAGT.
OrthoDBEOG6G4VQG.
PhylomeDBP30843.

Enzyme and pathway databases

BioCycEcoCyc:BASR-MONOMER.
ECOL316407:JW4074-MONOMER.
RETL1328306-WGS:GSTH-5535-MONOMER.

Gene expression databases

GenevestigatorP30843.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP30843.

Entry information

Entry nameBASR_ECOLI
AccessionPrimary (citable) accession number: P30843
Secondary accession number(s): Q2M6J1 expand/collapse secondary AC list , Q5MNC7, Q5MNC9, Q5MNE0, Q5MNE5, Q5MNF2, Q5MNH0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: August 29, 2003
Last modified: July 9, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene