Transcriptional regulatory protein BasR - Escherichia coli (strain K12)

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P30843 (BASR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein BasR

Gene names

Name:	basR
Synonyms:	pmrA
Ordered Locus Names:	b4113, JW4074

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Induction	The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions By similarity. Ref.2
Post-translational modification	Phosphorylated by BasS. Ref.6
Miscellaneous	The activity of the BasR protein is in some natural isolates, but not in strain K12, controlled at a post-transcriptional level by the PmrD protein under low Mg²⁺ concentration conditions.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Antibiotic resistance Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 16428424. Source: EcoCyc response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW response to iron(III) ion Inferred from mutant phenotype PubMed 12139617. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from direct assay Ref.6. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 222

222

Transcriptional regulatory protein BasR

PRO_0000081023

Regions

Domain

1 – 116

116

Response regulatory

Amino acid modifications

Modified residue

4-aspartylphosphate By similarity

Natural variations

Natural variant

S → G in strain: ECOR 1, ECOR 10, ECOR 11, ECOR 12, ECOR 13, ECOR 14, ECOR 15, ECOR 16, ECOR 17, ECOR 18, ECOR 19, ECOR 2, ECOR 20, ECOR 21, ECOR 22, ECOR 24, ECOR 25, ECOR 26, ECOR 27, ECOR 3, ECOR 30, ECOR 33, ECOR 34, ECOR 35, ECOR 36, ECOR 37, ECOR 38, ECOR 4, ECOR 40, ECOR 41, ECOR 42, ECOR 43, ECOR 44, ECOR 45, ECOR 46, ECOR 47, ECOR 48, ECOR 5, ECOR 50, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 6, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64, ECOR 65, ECOR 66, ECOR 67, ECOR 68, ECOR 69, ECOR 7, ECOR 70, ECOR 71, ECOR 72, ECOR 8 and ECOR 9.

Natural variant

T → S in strain: ECOR 35, ECOR 36, ECOR 38, ECOR 40, ECOR 41, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63 and ECOR 64, ECOR 65 and ECOR 66.

Natural variant

128

I → N in strain: ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63 and ECOR 64.

Natural variant

129

V → L in strain: ECOR 19.

Natural variant

144

G → S in strain: ECOR 44, ECOR 48, ECOR 50, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64 and ECOR 72.

Natural variant

207

R → P in strain: ECOR 27.

Natural variant

214

Y → F in strain: ECOR 27.

Experimental info

Sequence conflict

S → T Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P30843 [UniParc].

Last modified August 29, 2003. Version 3.
Checksum: 8588F9F23A1B8625
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FASTA

222

25,031

        10         20         30         40         50         60 
MKILIVEDDT LLLQGLILAA QTEGYACDSV TTARMAEQSL EAGHYSLVVL DLGLPDEDGL 

        70         80         90        100        110        120 
HFLARIRQKK YTLPVLILTA RDTLTDKIAG LDVGADDYLV KPFALEELHA RIRALLRRHN 

       130        140        150        160        170        180 
NQGESELIVG NLTLNMGRRQ VWMGGEELIL TPKEYALLSR LMLKAGSPVH REILYNDIYN 

       190        200        210        220 
WDNEPSTNTL EVHIHNLRDK VGKARIRTVR GFGYMLVANE EN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel members of the two-component signal transduction genes in Escherichia coli." Nagasawa S., Ishige K., Mizuno T. J. Biochem. 114:350-357(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Phenotypic differences between Salmonella and Escherichia coli resulting from the disparate regulation of homologous genes." Winfield M.D., Groisman E.A. Proc. Natl. Acad. Sci. U.S.A. 101:17162-17167(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REGULATION BY PMRD. Strain: ECOR 1, ECOR 10, ECOR 11, ECOR 12, ECOR 13, ECOR 14, ECOR 15, ECOR 16, ECOR 17, ECOR 18, ECOR 19, ECOR 2, ECOR 20, ECOR 21, ECOR 22, ECOR 24, ECOR 25, ECOR 26, ECOR 27, ECOR 3, ECOR 30, ECOR 33, ECOR 34, ECOR 35, ECOR 36, ECOR 37, ECOR 38, ECOR 4, ECOR 40, ECOR 41, ECOR 42, ECOR 43, ECOR 44, ECOR 45, ECOR 46, ECOR 47, ECOR 48, ECOR 5, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 6, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64, ECOR 65, ECOR 66, ECOR 67, ECOR 68, ECOR 69, ECOR 7, ECOR 70, ECOR 71, ECOR 72, ECOR 8, ECOR 9 and O2:HN / ECOR 50 / P97 / UPEC.
[3]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A. J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14055 Genomic DNA. Translation: BAA03143.1. AY725270 Genomic DNA. Translation: AAV92717.1. AY725271 Genomic DNA. Translation: AAV92718.1. AY725272 Genomic DNA. Translation: AAV92719.1. AY725273 Genomic DNA. Translation: AAV92720.1. AY725274 Genomic DNA. Translation: AAV92721.1. AY725275 Genomic DNA. Translation: AAV92722.1. AY725276 Genomic DNA. Translation: AAV92723.1. AY725277 Genomic DNA. Translation: AAV92724.1. AY725278 Genomic DNA. Translation: AAV92725.1. AY725279 Genomic DNA. Translation: AAV92726.1. AY725280 Genomic DNA. Translation: AAV92727.1. AY725281 Genomic DNA. Translation: AAV92728.1. AY725282 Genomic DNA. Translation: AAV92729.1. AY725283 Genomic DNA. Translation: AAV92730.1. AY725284 Genomic DNA. Translation: AAV92731.1. AY725285 Genomic DNA. Translation: AAV92732.1. AY725286 Genomic DNA. Translation: AAV92733.1. AY725287 Genomic DNA. Translation: AAV92734.1. AY725288 Genomic DNA. Translation: AAV92735.1. AY725289 Genomic DNA. Translation: AAV92736.1. AY725290 Genomic DNA. Translation: AAV92737.1. AY725291 Genomic DNA. Translation: AAV92738.1. AY725292 Genomic DNA. Translation: AAV92739.1. AY725293 Genomic DNA. Translation: AAV92740.1. AY725294 Genomic DNA. Translation: AAV92741.1. AY725295 Genomic DNA. Translation: AAV92742.1. AY725296 Genomic DNA. Translation: AAV92743.1. AY725297 Genomic DNA. Translation: AAV92744.1. AY725298 Genomic DNA. Translation: AAV92745.1. AY725299 Genomic DNA. Translation: AAV92746.1. AY725300 Genomic DNA. Translation: AAV92747.1. AY725301 Genomic DNA. Translation: AAV92748.1. AY725302 Genomic DNA. Translation: AAV92749.1. AY725303 Genomic DNA. Translation: AAV92750.1. AY725304 Genomic DNA. Translation: AAV92751.1. AY725305 Genomic DNA. Translation: AAV92752.1. AY725306 Genomic DNA. Translation: AAV92753.1. AY725307 Genomic DNA. Translation: AAV92754.1. AY725308 Genomic DNA. Translation: AAV92755.1. AY725309 Genomic DNA. Translation: AAV92756.1. AY725310 Genomic DNA. Translation: AAV92757.1. AY725311 Genomic DNA. Translation: AAV92758.1. AY725312 Genomic DNA. Translation: AAV92759.1. AY725313 Genomic DNA. Translation: AAV92760.1. AY725314 Genomic DNA. Translation: AAV92761.1. AY725315 Genomic DNA. Translation: AAV92762.1. AY725316 Genomic DNA. Translation: AAV92763.1. AY725317 Genomic DNA. Translation: AAV92764.1. AY725318 Genomic DNA. Translation: AAV92765.1. AY725319 Genomic DNA. Translation: AAV92766.1. AY725320 Genomic DNA. Translation: AAV92767.1. AY725321 Genomic DNA. Translation: AAV92768.1. AY725322 Genomic DNA. Translation: AAV92769.1. AY725323 Genomic DNA. Translation: AAV92770.1. AY725324 Genomic DNA. Translation: AAV92771.1. AY725325 Genomic DNA. Translation: AAV92772.1. AY725326 Genomic DNA. Translation: AAV92773.1. AY725327 Genomic DNA. Translation: AAV92774.1. AY725328 Genomic DNA. Translation: AAV92775.1. AY725329 Genomic DNA. Translation: AAV92776.1. AY725330 Genomic DNA. Translation: AAV92777.1. AY725331 Genomic DNA. Translation: AAV92778.1. AY725332 Genomic DNA. Translation: AAV92779.1. AY725333 Genomic DNA. Translation: AAV92780.1. U14003 Genomic DNA. Translation: AAA97012.1. U00096 Genomic DNA. Translation: AAC77074.1. AP009048 Genomic DNA. Translation: BAE78115.1.
PIR	H65220.
RefSeq	NP_418537.1. NC_000913.2. YP_492256.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P30843.
SMR	P30843. Positions 1-216.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9201N.
IntAct	P30843. 5 interactions.
STRING	511145.b4113.
Proteomic databases
PaxDb	P30843.
PRIDE	P30843.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77074; AAC77074; b4113. BAE78115; BAE78115; BAE78115.
GeneID	12934089. 948631.
KEGG	ecj:Y75_p4000. eco:b4113.
PATRIC	32123791. VBIEscCol129921_4244.
Organism-specific databases
EchoBASE	EB1572.
EcoGene	EG11615. basR.
Phylogenomic databases
eggNOG	COG0745.
KO	K07771.
OMA	GKATIRT.
ProtClustDB	PRK10643.
Enzyme and pathway databases
BioCyc	EcoCyc:BASR-MONOMER. ECOL316407:JW4074-MONOMER.
Gene expression databases
Genevestigator	P30843.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR011006. CheY-like_superfamily. IPR001867. Sig_transdc_resp-reg_C. IPR001789. Sig_transdc_resp-reg_receiver. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00072. Response_reg. 1 hit. PF00486. Trans_reg_C. 1 hit. [Graphical view]
SMART	SM00448. REC. 1 hit. SM00862. Trans_reg_C. 1 hit. [Graphical view]
SUPFAM	SSF52172. CheY_like. 1 hit.
PROSITE	PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BASR_ECOLI

Accession

Primary (citable) accession number: P30843
Secondary accession number(s): Q2M6J1

Q5MNH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	August 29, 2003
Last modified:	May 1, 2013
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents