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Protein

Transcriptional regulatory protein BasR

Gene

basR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin (By similarity).By similarity

Miscellaneous

The activity of the BasR protein is in some natural isolates, but not in strain K12, controlled at a post-transcriptional level by the PmrD protein under low Mg2+ concentration conditions.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi124 – 218OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST95

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • phosphorelay response regulator activity Source: EcoCyc

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • regulation of transcription, DNA-templated Source: EcoCyc
  • response to antibiotic Source: UniProtKB-KW
  • response to iron(III) ion Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processAntibiotic resistance, Transcription, Transcription regulation, Two-component regulatory system

Enzyme and pathway databases

BioCyciEcoCyc:BASR-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein BasR
Gene namesi
Name:basR
Synonyms:pmrA
Ordered Locus Names:b4113, JW4074
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11615 basR

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000810231 – 222Transcriptional regulatory protein BasRAdd BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei514-aspartylphosphatePROSITE-ProRule annotation1

Post-translational modificationi

Phosphorylated by BasS.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30843
PRIDEiP30843

Expressioni

Inductioni

The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions.By similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi42630837 interactors.
DIPiDIP-9201N
IntActiP30843 5 interactors.
STRINGi316385.ECDH10B_4305

Structurei

3D structure databases

ProteinModelPortaliP30843
SMRiP30843
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 116Response regulatoryPROSITE-ProRule annotationAdd BLAST115

Phylogenomic databases

eggNOGiENOG4105XJC Bacteria
ENOG4111FPD LUCA
InParanoidiP30843
KOiK07771
OMAiMEHPREV
PhylomeDBiP30843

Family and domain databases

CDDicd00156 REC, 1 hit
cd00383 trans_reg_C, 1 hit
Gene3Di1.10.10.101 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR001867 OmpR/PhoB-type_DNA-bd
IPR016032 Sig_transdc_resp-reg_C-effctor
IPR001789 Sig_transdc_resp-reg_receiver
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00072 Response_reg, 1 hit
PF00486 Trans_reg_C, 1 hit
SMARTiView protein in SMART
SM00448 REC, 1 hit
SM00862 Trans_reg_C, 1 hit
SUPFAMiSSF46894 SSF46894, 1 hit
SSF52172 SSF52172, 1 hit
PROSITEiView protein in PROSITE
PS51755 OMPR_PHOB, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

P30843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILIVEDDT LLLQGLILAA QTEGYACDSV TTARMAEQSL EAGHYSLVVL
60 70 80 90 100
DLGLPDEDGL HFLARIRQKK YTLPVLILTA RDTLTDKIAG LDVGADDYLV
110 120 130 140 150
KPFALEELHA RIRALLRRHN NQGESELIVG NLTLNMGRRQ VWMGGEELIL
160 170 180 190 200
TPKEYALLSR LMLKAGSPVH REILYNDIYN WDNEPSTNTL EVHIHNLRDK
210 220
VGKARIRTVR GFGYMLVANE EN
Length:222
Mass (Da):25,031
Last modified:August 29, 2003 - v3
Checksum:i8588F9F23A1B8625
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29S → T (PubMed:8282725).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti29S → G in strain: ECOR 1, ECOR 10, ECOR 11, ECOR 12, ECOR 13, ECOR 14, ECOR 15, ECOR 16, ECOR 17, ECOR 18, ECOR 19, ECOR 2, ECOR 20, ECOR 21, ECOR 22, ECOR 24, ECOR 25, ECOR 26, ECOR 27, ECOR 3, ECOR 30, ECOR 33, ECOR 34, ECOR 35, ECOR 36, ECOR 37, ECOR 38, ECOR 4, ECOR 40, ECOR 41, ECOR 42, ECOR 43, ECOR 44, ECOR 45, ECOR 46, ECOR 47, ECOR 48, ECOR 5, ECOR 50, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 6, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64, ECOR 65, ECOR 66, ECOR 67, ECOR 68, ECOR 69, ECOR 7, ECOR 70, ECOR 71, ECOR 72, ECOR 8 and ECOR 9. 1
Natural varianti31T → S in strain: ECOR 35, ECOR 36, ECOR 38, ECOR 40, ECOR 41, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63 and ECOR 64, ECOR 65 and ECOR 66. 1
Natural varianti128I → N in strain: ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63 and ECOR 64. 1
Natural varianti129V → L in strain: ECOR 19. 1
Natural varianti144G → S in strain: ECOR 44, ECOR 48, ECOR 50, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64 and ECOR 72. 1
Natural varianti207R → P in strain: ECOR 27. 1
Natural varianti214Y → F in strain: ECOR 27. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14055 Genomic DNA Translation: BAA03143.1
AY725270 Genomic DNA Translation: AAV92717.1
AY725271 Genomic DNA Translation: AAV92718.1
AY725272 Genomic DNA Translation: AAV92719.1
AY725273 Genomic DNA Translation: AAV92720.1
AY725274 Genomic DNA Translation: AAV92721.1
AY725275 Genomic DNA Translation: AAV92722.1
AY725276 Genomic DNA Translation: AAV92723.1
AY725277 Genomic DNA Translation: AAV92724.1
AY725278 Genomic DNA Translation: AAV92725.1
AY725279 Genomic DNA Translation: AAV92726.1
AY725280 Genomic DNA Translation: AAV92727.1
AY725281 Genomic DNA Translation: AAV92728.1
AY725282 Genomic DNA Translation: AAV92729.1
AY725283 Genomic DNA Translation: AAV92730.1
AY725284 Genomic DNA Translation: AAV92731.1
AY725285 Genomic DNA Translation: AAV92732.1
AY725286 Genomic DNA Translation: AAV92733.1
AY725287 Genomic DNA Translation: AAV92734.1
AY725288 Genomic DNA Translation: AAV92735.1
AY725289 Genomic DNA Translation: AAV92736.1
AY725290 Genomic DNA Translation: AAV92737.1
AY725291 Genomic DNA Translation: AAV92738.1
AY725292 Genomic DNA Translation: AAV92739.1
AY725293 Genomic DNA Translation: AAV92740.1
AY725294 Genomic DNA Translation: AAV92741.1
AY725295 Genomic DNA Translation: AAV92742.1
AY725296 Genomic DNA Translation: AAV92743.1
AY725297 Genomic DNA Translation: AAV92744.1
AY725298 Genomic DNA Translation: AAV92745.1
AY725299 Genomic DNA Translation: AAV92746.1
AY725300 Genomic DNA Translation: AAV92747.1
AY725301 Genomic DNA Translation: AAV92748.1
AY725302 Genomic DNA Translation: AAV92749.1
AY725303 Genomic DNA Translation: AAV92750.1
AY725304 Genomic DNA Translation: AAV92751.1
AY725305 Genomic DNA Translation: AAV92752.1
AY725306 Genomic DNA Translation: AAV92753.1
AY725307 Genomic DNA Translation: AAV92754.1
AY725308 Genomic DNA Translation: AAV92755.1
AY725309 Genomic DNA Translation: AAV92756.1
AY725310 Genomic DNA Translation: AAV92757.1
AY725311 Genomic DNA Translation: AAV92758.1
AY725312 Genomic DNA Translation: AAV92759.1
AY725313 Genomic DNA Translation: AAV92760.1
AY725314 Genomic DNA Translation: AAV92761.1
AY725315 Genomic DNA Translation: AAV92762.1
AY725316 Genomic DNA Translation: AAV92763.1
AY725317 Genomic DNA Translation: AAV92764.1
AY725318 Genomic DNA Translation: AAV92765.1
AY725319 Genomic DNA Translation: AAV92766.1
AY725320 Genomic DNA Translation: AAV92767.1
AY725321 Genomic DNA Translation: AAV92768.1
AY725322 Genomic DNA Translation: AAV92769.1
AY725323 Genomic DNA Translation: AAV92770.1
AY725324 Genomic DNA Translation: AAV92771.1
AY725325 Genomic DNA Translation: AAV92772.1
AY725326 Genomic DNA Translation: AAV92773.1
AY725327 Genomic DNA Translation: AAV92774.1
AY725328 Genomic DNA Translation: AAV92775.1
AY725329 Genomic DNA Translation: AAV92776.1
AY725330 Genomic DNA Translation: AAV92777.1
AY725331 Genomic DNA Translation: AAV92778.1
AY725332 Genomic DNA Translation: AAV92779.1
AY725333 Genomic DNA Translation: AAV92780.1
U14003 Genomic DNA Translation: AAA97012.1
U00096 Genomic DNA Translation: AAC77074.1
AP009048 Genomic DNA Translation: BAE78115.1
PIRiH65220
RefSeqiNP_418537.1, NC_000913.3
WP_000697926.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77074; AAC77074; b4113
BAE78115; BAE78115; BAE78115
GeneIDi948631
KEGGiecj:JW4074
eco:b4113
PATRICifig|1411691.4.peg.2587

Similar proteinsi

Entry informationi

Entry nameiBASR_ECOLI
AccessioniPrimary (citable) accession number: P30843
Secondary accession number(s): Q2M6J1
, Q5MNC7, Q5MNC9, Q5MNE0, Q5MNE5, Q5MNF2, Q5MNH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: August 29, 2003
Last modified: March 28, 2018
This is version 142 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome