Reviewed, UniProtKB/Swiss-Prot P30839 (AL3A2_RAT)

Names and origin

Protein names

Recommended name:
    Fatty aldehyde dehydrogenase
    EC=1.2.1.3
Alternative name(s):
    Aldehyde dehydrogenase family 3 member A2
    Aldehyde dehydrogenase 4
    Microsomal aldehyde dehydrogenase
      Short name=msALDH

Gene names

Name:	Aldh3a2
Synonyms:	Aldh4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	An aldehyde + NAD(+) + H(2)O = an acid + NADH.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular aldehyde metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aldehyde dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC aldehyde dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Fatty aldehyde dehydrogenase		PRO_0000056476
Regions
	Topological domain	1 – 463	463	Cytoplasmic
	Transmembrane	464 – 480	17	Potential
	Nucleotide binding	185 – 190	6	NAD Potential
	Motif	481 – 484	4	Prevents secretion from ER By similarity
Sites
	Active site	207	1	By similarity
	Active site	241	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30839-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 9F50CB34AB64C31D	FASTA	484	54,082
	10 20 30 40 50 60 MERQVQRLRQ TFRSGRSRPL RFRLQQLEAL RRMVQEREKD ILAAIAADLS KSELNAYSHE 70 80 90 100 110 120 VITILGEIDF MLGNLPELAS ARPAKKNLLT MMDEAYVQPE PLGVVLIIGA WNYPFVLTLQ 130 140 150 160 170 180 PLVGAIAAGN AAIVKPSELS ENTAKILAEL LPQYLDQDLY MIVNGGVEET TELLRQRFDH 190 200 210 220 230 240 ILYTGNTAVG KIVMEAAAKH LTPVTLELGG KSPCYIDRDC DLDVACRRIT WGKYMNCGQT 250 260 270 280 290 300 CIAPDYILCE ASSQDQIVQK IKDTVKDFYG ENVKASPDYE RIINLRHFKR IKSLLEGQKI 310 320 330 340 350 360 AFGGETDEAT RYIAPTILTD VDPNSKVMQE EIFGPILPIV SVKNVEEAIN FINDREKPLA 370 380 390 400 410 420 LYIFSHNNKL IKRVIDETSS GGVTGNDVIM HFTVNSLPFG GVGASGMGAY HGKYSFDTFS 430 440 450 460 470 480 HQRPCLLKGL KGESVNKLRY PPNSESKVSW SKFFLLKQFN KGRLQLLLLV CLVAVAAVIV KDQL

References

[1]	"Molecular cloning, sequencing, and expression of cDNA for rat liver microsomal aldehyde dehydrogenase." Miyauchi K., Masaki R., Taketani S., Yamamoto A., Akayama M., Tashiro Y. J. Biol. Chem. 266:19536-19542(1991) [PubMed: 1717467] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 93-110; 236-255 AND 408-427. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M73714 mRNA. Translation: AAA41555.1.
PIR	A41028.
RefSeq	NP_113919.2.
UniGene	Rn.9113
3D structure databases
HSSP	HSSP built from PDB template 1AD3 based on UniProtKB P11883.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000002342. Rattus norvegicus. [Contig view]
GeneID	65183.
KEGG	rno:65183.
Organism-specific databases
RGD	61866. Aldh3a2.
Phylogenomic databases
HOVERGEN	P30839.
Gene expression databases
ArrayExpress	P30839.
GermOnline	ENSRNOG00000002342. Rattus norvegicus.
Family and domain databases
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR012394. Ald_DHase_NAD(P). IPR015590. Aldehyde_DHase. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF15. ALDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF036492. ALDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	614089.

Entry information

Entry name

AL3A2_RAT

Accession

Primary (citable) accession number: P30839

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	November 25, 2008
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)