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Protein

Fatty aldehyde dehydrogenase

Gene

Aldh3a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid (By similarity).By similarity

Catalytic activityi

An aldehyde + NAD+ + H2O = a carboxylate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei207 – 2071By similarity
Active sitei241 – 2411By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi185 – 1906NADSequence Analysis

GO - Molecular functioni

  1. 3-chloroallyl aldehyde dehydrogenase activity Source: RGD
  2. aldehyde dehydrogenase (NAD) activity Source: GO_Central
  3. aldehyde dehydrogenase [NAD(P)+] activity Source: InterPro

GO - Biological processi

  1. formaldehyde metabolic process Source: RGD
  2. response to reactive oxygen species Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty aldehyde dehydrogenase (EC:1.2.1.3)
Alternative name(s):
Aldehyde dehydrogenase 4
Aldehyde dehydrogenase family 3 member A2
Microsomal aldehyde dehydrogenase
Short name:
msALDH
Gene namesi
Name:Aldh3a2
Synonyms:Aldh4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi61866. Aldh3a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 463463CytoplasmicAdd
BLAST
Transmembranei464 – 48017HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytosol Source: RGD
  2. endoplasmic reticulum Source: HGNC
  3. extrinsic component of endoplasmic reticulum membrane Source: RGD
  4. integral component of membrane Source: UniProtKB-KW
  5. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Fatty aldehyde dehydrogenasePRO_0000056476Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei293 – 2931PhosphoserineBy similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30839.
PRIDEiP30839.

Expressioni

Gene expression databases

GenevestigatoriP30839.

Interactioni

Protein-protein interaction databases

IntActiP30839. 1 interaction.
MINTiMINT-4564816.
STRINGi10116.ENSRNOP00000045886.

Structurei

3D structure databases

ProteinModelPortaliP30839.
SMRiP30839. Positions 5-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi481 – 4844Prevents secretion from ERBy similarity

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271515.
HOVERGENiHBG050483.
InParanoidiP30839.
KOiK00128.
PhylomeDBiP30839.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012394. Aldehyde_DH_NAD(P).
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036492. ALDH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30839-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERQVQRLRQ TFRSGRSRPL RFRLQQLEAL RRMVQEREKD ILAAIAADLS
60 70 80 90 100
KSELNAYSHE VITILGEIDF MLGNLPELAS ARPAKKNLLT MMDEAYVQPE
110 120 130 140 150
PLGVVLIIGA WNYPFVLTLQ PLVGAIAAGN AAIVKPSELS ENTAKILAEL
160 170 180 190 200
LPQYLDQDLY MIVNGGVEET TELLRQRFDH ILYTGNTAVG KIVMEAAAKH
210 220 230 240 250
LTPVTLELGG KSPCYIDRDC DLDVACRRIT WGKYMNCGQT CIAPDYILCE
260 270 280 290 300
ASSQDQIVQK IKDTVKDFYG ENVKASPDYE RIINLRHFKR IKSLLEGQKI
310 320 330 340 350
AFGGETDEAT RYIAPTILTD VDPNSKVMQE EIFGPILPIV SVKNVEEAIN
360 370 380 390 400
FINDREKPLA LYIFSHNNKL IKRVIDETSS GGVTGNDVIM HFTVNSLPFG
410 420 430 440 450
GVGASGMGAY HGKYSFDTFS HQRPCLLKGL KGESVNKLRY PPNSESKVSW
460 470 480
SKFFLLKQFN KGRLQLLLLV CLVAVAAVIV KDQL
Length:484
Mass (Da):54,082
Last modified:July 1, 1993 - v1
Checksum:i9F50CB34AB64C31D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73714 mRNA. Translation: AAA41555.1.
PIRiA41028.
RefSeqiNP_113919.2. NM_031731.2.
UniGeneiRn.9113.

Genome annotation databases

GeneIDi65183.
KEGGirno:65183.
UCSCiRGD:61866. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73714 mRNA. Translation: AAA41555.1.
PIRiA41028.
RefSeqiNP_113919.2. NM_031731.2.
UniGeneiRn.9113.

3D structure databases

ProteinModelPortaliP30839.
SMRiP30839. Positions 5-443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP30839. 1 interaction.
MINTiMINT-4564816.
STRINGi10116.ENSRNOP00000045886.

Proteomic databases

PaxDbiP30839.
PRIDEiP30839.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65183.
KEGGirno:65183.
UCSCiRGD:61866. rat.

Organism-specific databases

CTDi224.
RGDi61866. Aldh3a2.

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271515.
HOVERGENiHBG050483.
InParanoidiP30839.
KOiK00128.
PhylomeDBiP30839.

Miscellaneous databases

NextBioi614089.
PROiP30839.

Gene expression databases

GenevestigatoriP30839.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012394. Aldehyde_DH_NAD(P).
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036492. ALDH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning, sequencing, and expression of cDNA for rat liver microsomal aldehyde dehydrogenase."
    Miyauchi K., Masaki R., Taketani S., Yamamoto A., Akayama M., Tashiro Y.
    J. Biol. Chem. 266:19536-19542(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 93-110; 236-255 AND 408-427.
    Tissue: Liver.

Entry informationi

Entry nameiAL3A2_RAT
AccessioniPrimary (citable) accession number: P30839
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: March 4, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.