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Protein

Aldehyde dehydrogenase, dimeric NADP-preferring

Gene

ALDH3A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde (Probable). They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation (Probable). Oxidizes medium and long chain aldehydes into non-toxic fatty acids (PubMed:1737758). Preferentially oxidizes aromatic aldehyde substrates (PubMed:1737758). Comprises about 50 percent of corneal epithelial soluble proteins (By similarity). May play a role in preventing corneal damage caused by ultraviolet light (By similarity).By similarityCurated1 Publication

Catalytic activityi

An aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H.2 Publications

Kineticsi

Has a high Km for acetaldehyde.

      Sites

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Active sitei2101 Publication1
      Active sitei2441 Publication1

      Regions

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Nucleotide bindingi188 – 193NAD or NADPBy similarity6

      GO - Molecular functioni

      • 3-chloroallyl aldehyde dehydrogenase activity Source: GO_Central
      • alcohol dehydrogenase (NADP+) activity Source: UniProtKB
      • aldehyde dehydrogenase (NAD) activity Source: UniProtKB
      • aldehyde dehydrogenase [NAD(P)+] activity Source: Reactome
      • benzaldehyde dehydrogenase (NAD+) activity Source: CACAO

      GO - Biological processi

      Complete GO annotation...

      Keywords - Molecular functioni

      Oxidoreductase

      Keywords - Ligandi

      NAD, NADP

      Enzyme and pathway databases

      BioCyciZFISH:HS03133-MONOMER.
      BRENDAi1.2.1.5. 2681.
      ReactomeiR-HSA-211945. Phase 1 - Functionalization of compounds.
      SABIO-RKP30838.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Aldehyde dehydrogenase, dimeric NADP-preferring (EC:1.2.1.52 Publications)
      Alternative name(s):
      ALDHIII
      Aldehyde dehydrogenase 3
      Aldehyde dehydrogenase family 3 member A1
      Gene namesi
      Name:ALDH3A1
      Synonyms:ALDH3
      OrganismiHomo sapiens (Human)
      Taxonomic identifieri9606 [NCBI]
      Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
      Proteomesi
      • UP000005640 Componenti: Chromosome 17

      Organism-specific databases

      HGNCiHGNC:405. ALDH3A1.

      Subcellular locationi

      • Cytoplasm By similarity

      GO - Cellular componenti

      Complete GO annotation...

      Keywords - Cellular componenti

      Cytoplasm

      Pathology & Biotechi

      Mutagenesis

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Mutagenesisi244C → S: Abolishes activity. 1 Publication1

      Organism-specific databases

      DisGeNETi218.
      OpenTargetsiENSG00000108602.
      PharmGKBiPA24697.

      Chemistry databases

      ChEMBLiCHEMBL3578.

      Polymorphism and mutation databases

      BioMutaiALDH3A1.
      DMDMi311033473.

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Initiator methionineiRemovedCombined sources
      ChainiPRO_00000564702 – 453Aldehyde dehydrogenase, dimeric NADP-preferringAdd BLAST452

      Amino acid modifications

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Modified residuei2N-acetylserineCombined sources1
      Modified residuei178N6-acetyllysineCombined sources1
      Modified residuei194N6-acetyllysineCombined sources1

      Keywords - PTMi

      Acetylation

      Proteomic databases

      MaxQBiP30838.
      PaxDbiP30838.
      PeptideAtlasiP30838.
      PRIDEiP30838.

      PTM databases

      iPTMnetiP30838.
      PhosphoSitePlusiP30838.

      Expressioni

      Tissue specificityi

      High levels in stomach, esophagus and lung; low level in the liver and kidney.

      Gene expression databases

      BgeeiENSG00000108602.
      CleanExiHS_ALDH3A1.
      ExpressionAtlasiP30838. baseline and differential.
      GenevisibleiP30838. HS.

      Organism-specific databases

      HPAiCAB045957.
      HPA051150.
      HPA063783.

      Interactioni

      Subunit structurei

      Homodimer.1 Publication

      Binary interactionsi

      WithEntry#Exp.IntActNotes
      POT1Q9NUX52EBI-3905126,EBI-752420

      Protein-protein interaction databases

      BioGridi106720. 18 interactors.
      IntActiP30838. 3 interactors.
      STRINGi9606.ENSP00000225740.

      Chemistry databases

      BindingDBiP30838.

      Structurei

      Secondary structure

      1453
      Legend: HelixTurnBeta strandPDB Structure known for this area
      Show more details
      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Helixi3 – 16Combined sources14
      Turni17 – 20Combined sources4
      Helixi23 – 39Combined sources17
      Helixi41 – 52Combined sources12
      Helixi56 – 61Combined sources6
      Helixi64 – 82Combined sources19
      Helixi91 – 93Combined sources3
      Beta strandi96 – 104Combined sources9
      Beta strandi106 – 111Combined sources6
      Beta strandi114 – 116Combined sources3
      Helixi119 – 130Combined sources12
      Beta strandi134 – 138Combined sources5
      Beta strandi141 – 143Combined sources3
      Helixi144 – 157Combined sources14
      Turni160 – 162Combined sources3
      Helixi170 – 176Combined sources7
      Beta strandi182 – 188Combined sources7
      Helixi190 – 201Combined sources12
      Turni202 – 204Combined sources3
      Beta strandi207 – 210Combined sources4
      Beta strandi216 – 219Combined sources4
      Helixi225 – 237Combined sources13
      Helixi238 – 241Combined sources4
      Beta strandi249 – 252Combined sources4
      Helixi254 – 256Combined sources3
      Helixi257 – 272Combined sources16
      Helixi276 – 278Combined sources3
      Helixi288 – 298Combined sources11
      Beta strandi301 – 305Combined sources5
      Turni311 – 314Combined sources4
      Beta strandi319 – 323Combined sources5
      Helixi329 – 331Combined sources3
      Beta strandi337 – 344Combined sources8
      Helixi348 – 357Combined sources10
      Beta strandi362 – 367Combined sources6
      Helixi371 – 380Combined sources10
      Beta strandi384 – 389Combined sources6
      Helixi393 – 395Combined sources3
      Helixi406 – 408Combined sources3
      Helixi416 – 421Combined sources6
      Beta strandi423 – 430Combined sources8
      Helixi437 – 442Combined sources6
      Beta strandi443 – 445Combined sources3

      3D structure databases

      Select the link destinations:
      PDBei
      RCSB PDBi
      PDBji
      Links Updated
      PDB entryMethodResolution (Å)ChainPositionsPDBsum
      3SZAX-ray1.48A/B1-453[»]
      3SZBX-ray1.51A/B1-453[»]
      4H80X-ray2.50A/B/C/D/E/F/G/H1-453[»]
      4L1OX-ray2.30A/B1-453[»]
      4L2OX-ray1.94A/B/E/G1-453[»]
      ProteinModelPortaliP30838.
      SMRiP30838.
      ModBaseiSearch...
      MobiDBiSearch...

      Miscellaneous databases

      EvolutionaryTraceiP30838.

      Family & Domainsi

      Sequence similaritiesi

      Belongs to the aldehyde dehydrogenase family.Curated

      Phylogenomic databases

      eggNOGiKOG2456. Eukaryota.
      COG1012. LUCA.
      GeneTreeiENSGT00390000002825.
      HOGENOMiHOG000271515.
      HOVERGENiHBG050483.
      InParanoidiP30838.
      KOiK00129.
      OMAiINQHEKP.
      OrthoDBiEOG091G05HC.
      PhylomeDBiP30838.
      TreeFamiTF314264.

      Family and domain databases

      Gene3Di3.40.309.10. 1 hit.
      3.40.605.10. 1 hit.
      InterProiIPR016161. Ald_DH/histidinol_DH.
      IPR016163. Ald_DH_C.
      IPR016160. Ald_DH_CS_CYS.
      IPR029510. Ald_DH_CS_GLU.
      IPR016162. Ald_DH_N.
      IPR015590. Aldehyde_DH_dom.
      IPR012394. Aldehyde_DH_NAD(P).
      [Graphical view]
      PfamiPF00171. Aldedh. 1 hit.
      [Graphical view]
      PIRSFiPIRSF036492. ALDH. 1 hit.
      SUPFAMiSSF53720. SSF53720. 1 hit.
      PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
      PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
      [Graphical view]

      Sequencei

      Sequence statusi: Complete.

      Sequence processingi: The displayed sequence is further processed into a mature form.

      P30838-1 [UniParc]FASTAAdd to basket

      « Hide

              10         20         30         40         50
      MSKISEAVKR ARAAFSSGRT RPLQFRIQQL EALQRLIQEQ EQELVGALAA
      60 70 80 90 100
      DLHKNEWNAY YEEVVYVLEE IEYMIQKLPE WAADEPVEKT PQTQQDELYI
      110 120 130 140 150
      HSEPLGVVLV IGTWNYPFNL TIQPMVGAIA AGNSVVLKPS ELSENMASLL
      160 170 180 190 200
      ATIIPQYLDK DLYPVINGGV PETTELLKER FDHILYTGST GVGKIIMTAA
      210 220 230 240 250
      AKHLTPVTLE LGGKSPCYVD KNCDLDVACR RIAWGKFMNS GQTCVAPDYI
      260 270 280 290 300
      LCDPSIQNQI VEKLKKSLKE FYGEDAKKSR DYGRIISARH FQRVMGLIEG
      310 320 330 340 350
      QKVAYGGTGD AATRYIAPTI LTDVDPQSPV MQEEIFGPVL PIVCVRSLEE
      360 370 380 390 400
      AIQFINQREK PLALYMFSSN DKVIKKMIAE TSSGGVAAND VIVHITLHSL
      410 420 430 440 450
      PFGGVGNSGM GSYHGKKSFE TFSHRRSCLV RPLMNDEGLK VRYPPSPAKM

      TQH
      Length:453
      Mass (Da):50,395
      Last modified:November 2, 2010 - v3
      Checksum:iD35C488A022FAACA
      GO

      Experimental Info

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Sequence conflicti12R → P in AAB46377 (PubMed:1737758).Curated1
      Sequence conflicti12R → P in AAB26658 (PubMed:8493892).Curated1
      Sequence conflicti27I → F in AAA51696 (Ref. 1) Curated1
      Sequence conflicti170V → L AA sequence (PubMed:2037078).Curated1
      Sequence conflicti436D → E AA sequence (PubMed:2037078).Curated1

      Natural variant

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Natural variantiVAR_018981134S → A.5 PublicationsCorresponds to variant rs887241dbSNPEnsembl.1
      Natural variantiVAR_018982309G → E.Corresponds to variant rs3744692dbSNPEnsembl.1
      Natural variantiVAR_011303329P → A in allele ALDH3A1*2. 1 PublicationCorresponds to variant rs2228100dbSNPEnsembl.1

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      M74542 mRNA. Translation: AAA51696.1.
      M77477 mRNA. Translation: AAB46377.1.
      S61044 mRNA. Translation: AAB26658.1.
      BT007102 mRNA. Translation: AAP35766.1.
      AK292193 mRNA. Translation: BAF84882.1.
      AK314584 mRNA. Translation: BAG37160.1.
      AC005722 Genomic DNA. No translation available.
      CH471212 Genomic DNA. Translation: EAW50909.1.
      BC004370 mRNA. Translation: AAH04370.1.
      BC008892 mRNA. Translation: AAH08892.1.
      BC021194 mRNA. Translation: AAH21194.1.
      CCDSiCCDS11212.1.
      PIRiA42584.
      RefSeqiNP_000682.3. NM_000691.4.
      NP_001128639.1. NM_001135167.1.
      NP_001128640.1. NM_001135168.1.
      UniGeneiHs.531682.

      Genome annotation databases

      EnsembliENST00000225740; ENSP00000225740; ENSG00000108602.
      ENST00000444455; ENSP00000388469; ENSG00000108602.
      ENST00000457500; ENSP00000411821; ENSG00000108602.
      GeneIDi218.
      KEGGihsa:218.
      UCSCiuc002gwj.4. human.

      Keywords - Coding sequence diversityi

      Polymorphism

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      M74542 mRNA. Translation: AAA51696.1.
      M77477 mRNA. Translation: AAB46377.1.
      S61044 mRNA. Translation: AAB26658.1.
      BT007102 mRNA. Translation: AAP35766.1.
      AK292193 mRNA. Translation: BAF84882.1.
      AK314584 mRNA. Translation: BAG37160.1.
      AC005722 Genomic DNA. No translation available.
      CH471212 Genomic DNA. Translation: EAW50909.1.
      BC004370 mRNA. Translation: AAH04370.1.
      BC008892 mRNA. Translation: AAH08892.1.
      BC021194 mRNA. Translation: AAH21194.1.
      CCDSiCCDS11212.1.
      PIRiA42584.
      RefSeqiNP_000682.3. NM_000691.4.
      NP_001128639.1. NM_001135167.1.
      NP_001128640.1. NM_001135168.1.
      UniGeneiHs.531682.

      3D structure databases

      Select the link destinations:
      PDBei
      RCSB PDBi
      PDBji
      Links Updated
      PDB entryMethodResolution (Å)ChainPositionsPDBsum
      3SZAX-ray1.48A/B1-453[»]
      3SZBX-ray1.51A/B1-453[»]
      4H80X-ray2.50A/B/C/D/E/F/G/H1-453[»]
      4L1OX-ray2.30A/B1-453[»]
      4L2OX-ray1.94A/B/E/G1-453[»]
      ProteinModelPortaliP30838.
      SMRiP30838.
      ModBaseiSearch...
      MobiDBiSearch...

      Protein-protein interaction databases

      BioGridi106720. 18 interactors.
      IntActiP30838. 3 interactors.
      STRINGi9606.ENSP00000225740.

      Chemistry databases

      BindingDBiP30838.
      ChEMBLiCHEMBL3578.

      PTM databases

      iPTMnetiP30838.
      PhosphoSitePlusiP30838.

      Polymorphism and mutation databases

      BioMutaiALDH3A1.
      DMDMi311033473.

      Proteomic databases

      MaxQBiP30838.
      PaxDbiP30838.
      PeptideAtlasiP30838.
      PRIDEiP30838.

      Protocols and materials databases

      DNASUi218.
      Structural Biology KnowledgebaseSearch...

      Genome annotation databases

      EnsembliENST00000225740; ENSP00000225740; ENSG00000108602.
      ENST00000444455; ENSP00000388469; ENSG00000108602.
      ENST00000457500; ENSP00000411821; ENSG00000108602.
      GeneIDi218.
      KEGGihsa:218.
      UCSCiuc002gwj.4. human.

      Organism-specific databases

      CTDi218.
      DisGeNETi218.
      GeneCardsiALDH3A1.
      H-InvDBHIX0013622.
      HGNCiHGNC:405. ALDH3A1.
      HPAiCAB045957.
      HPA051150.
      HPA063783.
      MIMi100660. gene.
      neXtProtiNX_P30838.
      OpenTargetsiENSG00000108602.
      PharmGKBiPA24697.
      GenAtlasiSearch...

      Phylogenomic databases

      eggNOGiKOG2456. Eukaryota.
      COG1012. LUCA.
      GeneTreeiENSGT00390000002825.
      HOGENOMiHOG000271515.
      HOVERGENiHBG050483.
      InParanoidiP30838.
      KOiK00129.
      OMAiINQHEKP.
      OrthoDBiEOG091G05HC.
      PhylomeDBiP30838.
      TreeFamiTF314264.

      Enzyme and pathway databases

      BioCyciZFISH:HS03133-MONOMER.
      BRENDAi1.2.1.5. 2681.
      ReactomeiR-HSA-211945. Phase 1 - Functionalization of compounds.
      SABIO-RKP30838.

      Miscellaneous databases

      ChiTaRSiALDH3A1. human.
      EvolutionaryTraceiP30838.
      GeneWikiiAldehyde_dehydrogenase_3_family,_member_A1.
      GenomeRNAii218.
      PROiP30838.
      SOURCEiSearch...

      Gene expression databases

      BgeeiENSG00000108602.
      CleanExiHS_ALDH3A1.
      ExpressionAtlasiP30838. baseline and differential.
      GenevisibleiP30838. HS.

      Family and domain databases

      Gene3Di3.40.309.10. 1 hit.
      3.40.605.10. 1 hit.
      InterProiIPR016161. Ald_DH/histidinol_DH.
      IPR016163. Ald_DH_C.
      IPR016160. Ald_DH_CS_CYS.
      IPR029510. Ald_DH_CS_GLU.
      IPR016162. Ald_DH_N.
      IPR015590. Aldehyde_DH_dom.
      IPR012394. Aldehyde_DH_NAD(P).
      [Graphical view]
      PfamiPF00171. Aldedh. 1 hit.
      [Graphical view]
      PIRSFiPIRSF036492. ALDH. 1 hit.
      SUPFAMiSSF53720. SSF53720. 1 hit.
      PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
      PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
      [Graphical view]
      ProtoNetiSearch...

      Entry informationi

      Entry nameiAL3A1_HUMAN
      AccessioniPrimary (citable) accession number: P30838
      Secondary accession number(s): A8K828, Q9BT37
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: July 1, 1993
      Last sequence update: November 2, 2010
      Last modified: November 2, 2016
      This is version 164 of the entry and version 3 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programChordata Protein Annotation Program
      DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

      Miscellaneousi

      Keywords - Technical termi

      3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

      Documents

      1. Human chromosome 17
        Human chromosome 17: entries, gene names and cross-references to MIM
      2. Human entries with polymorphisms or disease mutations
        List of human entries with polymorphisms or disease mutations
      3. Human polymorphisms and disease mutations
        Index of human polymorphisms and disease mutations
      4. MIM cross-references
        Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
      5. PDB cross-references
        Index of Protein Data Bank (PDB) cross-references
      6. SIMILARITY comments
        Index of protein domains and families

      Similar proteinsi

      Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
      100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
      90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
      50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.