Skip Header

Contribute Send feedback
Read comments (?) or add your own

P30836 (LYAM1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
L-selectin
Alternative name(s):
CD62 antigen-like family member L
Leukocyte adhesion molecule 1
Short name=LAM-1
Leukocyte-endothelial cell adhesion molecule 1
Short name=LECAM1
Lymph node homing receptor
Lymphocyte antigen 22
Short name=Ly-22
Lymphocyte surface MEL-14 antigen
CD_antigen=CD62L
Gene names
Name:Sell
Synonyms:Lnhr, Ly-22
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia By similarity.

Subunit structure

Interaction with PSGL1/SELPLG and PODXL2 is required for promoting recruitment and rolling of leukocytes. This interaction is dependent on the sialyl Lewis X glycan modification of PSGL1 and PODXL2, and tyrosine sulfation modifications of PSGL1. Sulfation on 'Tyr-51' of PSGL1 is important for L-selectin binding By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed in peripheral blood mononuclear cells (PBMC), spleen and thymus. Ref.1

Induction

Down-regulated by mitogen stimulation of PBMC and spleen T-cells. Ref.1

Sequence similarities

Belongs to the selectin/LECAM family.

Contains 1 C-type lectin domain.

Contains 1 EGF-like domain.

Contains 2 Sushi (CCP/SCR) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 By similarity
Propeptide29 – 3810 By similarity
PRO_0000017487
Chain39 – 372334L-selectin
PRO_0000017488

Regions

Topological domain39 – 332294Extracellular Potential
Transmembrane333 – 35523Helical; Potential
Topological domain356 – 37217Cytoplasmic Potential
Domain55 – 155101C-type lectin
Domain156 – 19237EGF-like
Domain195 – 25662Sushi 1
Domain257 – 31862Sushi 2

Amino acid modifications

Glycosylation601N-linked (GlcNAc...) Potential
Glycosylation1041N-linked (GlcNAc...) Potential
Glycosylation1771N-linked (GlcNAc...) Potential
Glycosylation2261N-linked (GlcNAc...) Potential
Glycosylation2461N-linked (GlcNAc...) Potential
Glycosylation2781N-linked (GlcNAc...) Potential
Disulfide bond57 ↔ 155 By similarity
Disulfide bond128 ↔ 147 By similarity
Disulfide bond160 ↔ 171 By similarity
Disulfide bond165 ↔ 180 By similarity
Disulfide bond182 ↔ 191 By similarity
Disulfide bond197 ↔ 241 By similarity
Disulfide bond227 ↔ 254 By similarity
Disulfide bond259 ↔ 303 By similarity
Disulfide bond289 ↔ 316 By similarity

Sequences

Sequence LengthMass (Da)Tools
P30836 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 3B88AE0F1E4D191A

FASTA37242,441
        10         20         30         40         50         60 
MVFPWRCQSA QRGSWSFLKL WIRTLLCCDL LPHHGTHCWT YHYSERSMNW ENARKFCKHN 

        70         80         90        100        110        120 
YTDLVAIQNK REIEYLEKTL PKNPTYYWIG IRKIGKTWTW VGTNKTLTKE AENWGTGEPN 

       130        140        150        160        170        180 
NKKSKEDCVE IYIKRERDSG KWNDDACHKR KAALCYTASC QPESCNRHGE CVETINNNTC 

       190        200        210        220        230        240 
ICDPGYYGPQ CQYVIQCEPL KAPELGTMNC IHPLGDFSFQ SQCAFNCSEG SELLGNAKTE 

       250        260        270        280        290        300 
CGASGNWTYL EPICQVIQCM PLAAPDLGTM ECSHPLANFS FTSACTFTCS EETDLIGERK 

       310        320        330        340        350        360 
TVCRSSGSWS SPSPICQKTK RSFSKIKEGD YNPLFIPVAV MVTAFSGLAF IIWLARRLKK 

       370 
GKKSQERMDD PY

« Hide

References

[1]"Sequence and expression of a rat cDNA for LECAM-1."
Watanabe T., Song Y., Hirayama Y., Tamatani T., Kuida K., Miyasaka M.
Biochim. Biophys. Acta 1131:321-324(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D10831 mRNA. Translation: BAA01613.1.
IPIIPI00327594.
PIRS23936.
UniGeneRn.10461.

3D structure databases

ProteinModelPortalP30836.
SMRP30836. Positions 39-194.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000003733.

Proteomic databases

PaxDbP30836.
PRIDEP30836.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:3655. rat.

Organism-specific databases

RGD3655. Sell.

Phylogenomic databases

eggNOGNOG258998.
HOGENOMHOG000236254.
HOVERGENHBG052375.
InParanoidP30836.
OrthoDBEOG4FFD22.

Gene expression databases

ArrayExpressP30836.
GenevestigatorP30836.
GermOnlineENSRNOG00000002776. Rattus norvegicus.

Family and domain databases

Gene3D3.10.100.10. 1 hit.
InterProIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR013111. EGF_extracell.
IPR016348. L-selectin.
IPR002396. Selectin_superfamily.
IPR000436. Sushi_SCR_CCP.
[Graphical view]
PfamPF07974. EGF_2. 1 hit.
PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 2 hits.
[Graphical view]
PIRSFPIRSF002421. L-selectin. 1 hit.
PRINTSPR00343. SELECTIN.
SMARTSM00032. CCP. 2 hits.
SM00034. CLECT. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMSSF56436. C-type_lectin_fold. 1 hit.
SSF57535. Complement_control_module. 2 hits.
PROSITEPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50923. SUSHI. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLYAM1_RAT
AccessionPrimary (citable) accession number: P30836
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents