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Protein

ATP-dependent 6-phosphofructokinase, liver type

Gene

Pfkl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate. GlcNAcylation by OGT overcomes allosteric regulation (By similarity).UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251ATP; via amide nitrogenUniRule annotation
Metal bindingi119 – 1191Magnesium; catalyticUniRule annotation
Active sitei166 – 1661Proton acceptorUniRule annotation
Binding sitei201 – 2011Substrate; shared with dimeric partnerUniRule annotation
Binding sitei264 – 2641SubstrateUniRule annotation
Binding sitei292 – 2921Substrate; shared with dimeric partnerUniRule annotation
Binding sitei470 – 4701Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei565 – 5651Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei628 – 6281Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei654 – 6541Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei734 – 7341Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi88 – 892ATPUniRule annotation
Nucleotide bindingi118 – 1214ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: RGD
  2. ATP binding Source: RGD
  3. fructose-2,6-bisphosphate 2-phosphatase activity Source: RGD
  4. fructose-6-phosphate binding Source: RGD
  5. fructose binding Source: Ensembl
  6. metal ion binding Source: UniProtKB-KW
  7. monosaccharide binding Source: RGD

GO - Biological processi

  1. carbohydrate metabolic process Source: RGD
  2. carbohydrate phosphorylation Source: GOC
  3. dephosphorylation Source: GOC
  4. fructose 1,6-bisphosphate metabolic process Source: RGD
  5. fructose 6-phosphate metabolic process Source: RGD
  6. glycolytic process Source: RGD
  7. negative regulation of insulin secretion Source: Ensembl
  8. protein homotetramerization Source: RGD
  9. response to glucose Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_225694. Glycolysis.
SABIO-RKP30835.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase, liver typeUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PFK-L
Alternative name(s):
6-phosphofructokinase type B
Phosphofructo-1-kinase isozyme B
Short name:
PFK-B
PhosphohexokinaseUniRule annotation
Gene namesi
Name:Pfkl
Synonyms:Pfk-l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 20

Organism-specific databases

RGDi3311. Pfkl.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: Ensembl
  2. cytosol Source: RGD
  3. extracellular vesicular exosome Source: Ensembl
  4. membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 780779ATP-dependent 6-phosphofructokinase, liver typePRO_0000112023Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Glycosylationi529 – 5291O-linked (GlcNAc)By similarity
Modified residuei640 – 6401PhosphotyrosineBy similarity
Modified residuei775 – 7751PhosphoserineBy similarity

Post-translational modificationi

GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation (By similarity).By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP30835.
PRIDEiP30835.

PTM databases

PhosphoSiteiP30835.

Expressioni

Gene expression databases

GenevestigatoriP30835.

Interactioni

Subunit structurei

Homo- and heterotetramers.

Protein-protein interaction databases

BioGridi247769. 1 interaction.
IntActiP30835. 1 interaction.
MINTiMINT-4575401.
STRINGi10116.ENSRNOP00000001625.

Structurei

3D structure databases

ProteinModelPortaliP30835.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 390389N-terminal catalytic PFK domain 1Add
BLAST
Regioni164 – 1663Substrate bindingUniRule annotation
Regioni208 – 2103Substrate bindingUniRule annotation
Regioni298 – 3014Substrate bindingUniRule annotation
Regioni391 – 40010Interdomain linker
Regioni401 – 780380C-terminal regulatory PFK domain 2Add
BLAST
Regioni527 – 5315Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni572 – 5743Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni660 – 6634Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
GeneTreeiENSGT00390000013209.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.
InParanoidiP30835.
KOiK00850.
OMAiVQHGITN.
OrthoDBiEOG7ZSHV5.
PhylomeDBiP30835.
TreeFamiTF300411.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30835-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATVDLEKLR MSGAGKAIGV LTSGGDAQGM NAAVRAVTRM GIYVGAKVFL
60 70 80 90 100
IYEGYEGLVE GGENIKPANW LSVSNIIQLG GTIIGSARCK AFTTREGRLA
110 120 130 140 150
AAYNLLQHGI TNLCVIGGDG SLTGANIFRN EWGSLLEELV KEGKISESTA
160 170 180 190 200
QNYAHLSIAG LVGSIDNDFC GTDMTIGTDS ALHRIMEVID AITTTAQSHQ
210 220 230 240 250
RTFVLEVMGR HCGYLALVSA LASGADWLFI PEAPPEDGWE NFMCERLGET
260 270 280 290 300
RSRGSRLNII IIAEGAIDRH GKPISSSYVK DLVVQRLGFD TRVTVLGHVQ
310 320 330 340 350
RGGTPSAFDR VLSSKMGMEA VMALLEATPD TPACVVSLSG NQSVRLPLME
360 370 380 390 400
CVQVTKDVQK AMDEKRFDEA IQLRGRSFEN NWKIYKLLAH QKVSKEKSNF
410 420 430 440 450
SLAILNVGAP AAGMNAAVRS AVRTGISEGH TVYVVHDGFE GLAKGQVQEV
460 470 480 490 500
GWHDVAGWLG RGGSMLGTKR TLPKPHLEAI VENLRTYNIH ALLVIGGFEA
510 520 530 540 550
YEGVLQLVEA RGRYEELCIV MCVIPATISN NVPGTDFSLG SDTAVNAAME
560 570 580 590 600
SCDRIKQSAS GTKRRVFIVE TMGGYCGYLA TVTGIAVGAD AAYVFEDPFN
610 620 630 640 650
IHDLKANVEH MTEKMKTDIQ RGLVLRNEKC HEHYTTEFLY NLYSSEGRGV
660 670 680 690 700
FDCRTNVLGH LQQGGAPTPF DRNYGTKLGV KAMLWMSEKL RDVYRKGRVF
710 720 730 740 750
ANAPDSACVI GLRKKVVAFS SVTELKKETD FEHRMPREQW WLNLRLMLKM
760 770 780
LAHYRISMAD YVSGELEHVT RRTLSIDKGF
Length:780
Mass (Da):85,339
Last modified:January 23, 2007 - v3
Checksum:i7F6FCFB076D6A0F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58865 mRNA. Translation: CAA41674.1.
PIRiS19689.
RefSeqiNP_037322.1. NM_013190.4.
UniGeneiRn.4212.

Genome annotation databases

EnsembliENSRNOT00000001625; ENSRNOP00000001625; ENSRNOG00000001214.
GeneIDi25741.
KEGGirno:25741.
UCSCiRGD:3311. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58865 mRNA. Translation: CAA41674.1.
PIRiS19689.
RefSeqiNP_037322.1. NM_013190.4.
UniGeneiRn.4212.

3D structure databases

ProteinModelPortaliP30835.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247769. 1 interaction.
IntActiP30835. 1 interaction.
MINTiMINT-4575401.
STRINGi10116.ENSRNOP00000001625.

PTM databases

PhosphoSiteiP30835.

Proteomic databases

PaxDbiP30835.
PRIDEiP30835.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001625; ENSRNOP00000001625; ENSRNOG00000001214.
GeneIDi25741.
KEGGirno:25741.
UCSCiRGD:3311. rat.

Organism-specific databases

CTDi5211.
RGDi3311. Pfkl.

Phylogenomic databases

eggNOGiCOG0205.
GeneTreeiENSGT00390000013209.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.
InParanoidiP30835.
KOiK00850.
OMAiVQHGITN.
OrthoDBiEOG7ZSHV5.
PhylomeDBiP30835.
TreeFamiTF300411.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
ReactomeiREACT_225694. Glycolysis.
SABIO-RKP30835.

Miscellaneous databases

NextBioi607897.
PROiP30835.

Gene expression databases

GenevestigatoriP30835.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rat-liver-type phosphofructokinase mRNA. Structure, tissue distribution and regulation."
    Hotta K., Nakajima H., Yamasaki T., Hamaguchi T., Kuwajima M., Noguchi T., Tanaka T., Kono N., Tarui S.
    Eur. J. Biochem. 202:293-298(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.

Entry informationi

Entry nameiPFKAL_RAT
AccessioniPrimary (citable) accession number: P30835
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.