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P30835

- PFKAL_RAT

UniProt

P30835 - PFKAL_RAT

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Protein
ATP-dependent 6-phosphofructokinase, liver type
Gene
Pfkl, Pfk-l
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate. GlcNAcylation by OGT overcomes allosteric regulation By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251ATP; via amide nitrogen By similarity
Metal bindingi119 – 1191Magnesium; catalytic By similarity
Active sitei166 – 1661Proton acceptor By similarity
Binding sitei201 – 2011Substrate; shared with dimeric partner By similarity
Binding sitei264 – 2641Substrate By similarity
Binding sitei292 – 2921Substrate; shared with dimeric partner By similarity
Binding sitei470 – 4701Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei565 – 5651Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei628 – 6281Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei654 – 6541Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei734 – 7341Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi88 – 892ATP By similarity
Nucleotide bindingi118 – 1214ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: RGD
  2. ATP binding Source: RGD
  3. fructose binding Source: Ensembl
  4. fructose-2,6-bisphosphate 2-phosphatase activity Source: RGD
  5. fructose-6-phosphate binding Source: RGD
  6. metal ion binding Source: UniProtKB-KW
  7. monosaccharide binding Source: RGD

GO - Biological processi

  1. carbohydrate metabolic process Source: RGD
  2. carbohydrate phosphorylation Source: GOC
  3. dephosphorylation Source: GOC
  4. fructose 1,6-bisphosphate metabolic process Source: RGD
  5. fructose 6-phosphate metabolic process Source: RGD
  6. glycolytic process Source: RGD
  7. negative regulation of insulin secretion Source: Ensembl
  8. protein homotetramerization Source: RGD
  9. response to glucose Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_225694. Glycolysis.
SABIO-RKP30835.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase, liver type (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
PFK-L
Alternative name(s):
6-phosphofructokinase type B
Phosphofructo-1-kinase isozyme B
Short name:
PFK-B
Phosphohexokinase
Gene namesi
Name:Pfkl
Synonyms:Pfk-l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 20

Organism-specific databases

RGDi3311. Pfkl.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: Ensembl
  2. cytosol Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 780779ATP-dependent 6-phosphofructokinase, liver typeUniRule annotation
PRO_0000112023Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Glycosylationi529 – 5291O-linked (GlcNAc) By similarity
Modified residuei640 – 6401Phosphotyrosine By similarity
Modified residuei775 – 7751Phosphoserine By similarity

Post-translational modificationi

GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation By similarity.UniRule annotation

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP30835.
PRIDEiP30835.

PTM databases

PhosphoSiteiP30835.

Expressioni

Gene expression databases

GenevestigatoriP30835.

Interactioni

Subunit structurei

Homo- and heterotetramers.

Protein-protein interaction databases

BioGridi247769. 1 interaction.
IntActiP30835. 1 interaction.
MINTiMINT-4575401.
STRINGi10116.ENSRNOP00000001625.

Structurei

3D structure databases

ProteinModelPortaliP30835.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 390389N-terminal catalytic PFK domain 1UniRule annotation
Add
BLAST
Regioni164 – 1663Substrate binding By similarity
Regioni208 – 2103Substrate binding By similarity
Regioni298 – 3014Substrate binding By similarity
Regioni391 – 40010Interdomain linkerUniRule annotation
Regioni401 – 780380C-terminal regulatory PFK domain 2UniRule annotation
Add
BLAST
Regioni527 – 5315Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni572 – 5743Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni660 – 6634Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
GeneTreeiENSGT00390000013209.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.
InParanoidiP30835.
KOiK00850.
OMAiVQHGITN.
OrthoDBiEOG7ZSHV5.
PhylomeDBiP30835.
TreeFamiTF300411.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30835-1 [UniParc]FASTAAdd to Basket

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MATVDLEKLR MSGAGKAIGV LTSGGDAQGM NAAVRAVTRM GIYVGAKVFL    50
IYEGYEGLVE GGENIKPANW LSVSNIIQLG GTIIGSARCK AFTTREGRLA 100
AAYNLLQHGI TNLCVIGGDG SLTGANIFRN EWGSLLEELV KEGKISESTA 150
QNYAHLSIAG LVGSIDNDFC GTDMTIGTDS ALHRIMEVID AITTTAQSHQ 200
RTFVLEVMGR HCGYLALVSA LASGADWLFI PEAPPEDGWE NFMCERLGET 250
RSRGSRLNII IIAEGAIDRH GKPISSSYVK DLVVQRLGFD TRVTVLGHVQ 300
RGGTPSAFDR VLSSKMGMEA VMALLEATPD TPACVVSLSG NQSVRLPLME 350
CVQVTKDVQK AMDEKRFDEA IQLRGRSFEN NWKIYKLLAH QKVSKEKSNF 400
SLAILNVGAP AAGMNAAVRS AVRTGISEGH TVYVVHDGFE GLAKGQVQEV 450
GWHDVAGWLG RGGSMLGTKR TLPKPHLEAI VENLRTYNIH ALLVIGGFEA 500
YEGVLQLVEA RGRYEELCIV MCVIPATISN NVPGTDFSLG SDTAVNAAME 550
SCDRIKQSAS GTKRRVFIVE TMGGYCGYLA TVTGIAVGAD AAYVFEDPFN 600
IHDLKANVEH MTEKMKTDIQ RGLVLRNEKC HEHYTTEFLY NLYSSEGRGV 650
FDCRTNVLGH LQQGGAPTPF DRNYGTKLGV KAMLWMSEKL RDVYRKGRVF 700
ANAPDSACVI GLRKKVVAFS SVTELKKETD FEHRMPREQW WLNLRLMLKM 750
LAHYRISMAD YVSGELEHVT RRTLSIDKGF 780
Length:780
Mass (Da):85,339
Last modified:January 23, 2007 - v3
Checksum:i7F6FCFB076D6A0F1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58865 mRNA. Translation: CAA41674.1.
PIRiS19689.
RefSeqiNP_037322.1. NM_013190.4.
UniGeneiRn.4212.

Genome annotation databases

EnsembliENSRNOT00000001625; ENSRNOP00000001625; ENSRNOG00000001214.
GeneIDi25741.
KEGGirno:25741.
UCSCiRGD:3311. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58865 mRNA. Translation: CAA41674.1 .
PIRi S19689.
RefSeqi NP_037322.1. NM_013190.4.
UniGenei Rn.4212.

3D structure databases

ProteinModelPortali P30835.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247769. 1 interaction.
IntActi P30835. 1 interaction.
MINTi MINT-4575401.
STRINGi 10116.ENSRNOP00000001625.

PTM databases

PhosphoSitei P30835.

Proteomic databases

PaxDbi P30835.
PRIDEi P30835.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000001625 ; ENSRNOP00000001625 ; ENSRNOG00000001214 .
GeneIDi 25741.
KEGGi rno:25741.
UCSCi RGD:3311. rat.

Organism-specific databases

CTDi 5211.
RGDi 3311. Pfkl.

Phylogenomic databases

eggNOGi COG0205.
GeneTreei ENSGT00390000013209.
HOGENOMi HOG000200154.
HOVERGENi HBG000976.
InParanoidi P30835.
KOi K00850.
OMAi VQHGITN.
OrthoDBi EOG7ZSHV5.
PhylomeDBi P30835.
TreeFami TF300411.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
Reactomei REACT_225694. Glycolysis.
SABIO-RK P30835.

Miscellaneous databases

NextBioi 607897.
PROi P30835.

Gene expression databases

Genevestigatori P30835.

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Rat-liver-type phosphofructokinase mRNA. Structure, tissue distribution and regulation."
    Hotta K., Nakajima H., Yamasaki T., Hamaguchi T., Kuwajima M., Noguchi T., Tanaka T., Kono N., Tarui S.
    Eur. J. Biochem. 202:293-298(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.

Entry informationi

Entry nameiPFKAL_RAT
AccessioniPrimary (citable) accession number: P30835
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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