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P30822 (XPO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exportin-1
Alternative name(s):
Chromosome region maintenance protein 1
Karyopherin-124
Gene names
Name:CRM1
Synonyms:KAP124, XPO1
Ordered Locus Names:YGR218W
ORF Names:G8514
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1084 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for the leucine-rich nuclear export signal (NES).

Subunit structure

Interacts with GSP1/GSP2, polyadenylate-binding protein PAB1 and PRP40. Ref.8 Ref.9

Subcellular location

Nucleus. Cytoplasmperinuclear region. Note: Localized in the nucleus and at its periphery. Ref.6

Miscellaneous

Present with 7080 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the exportin family.

Contains 1 importin N-terminal domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PKIAP6192517EBI-20589,EBI-2682139From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10841084Exportin-1
PRO_0000204710

Regions

Domain34 – 10067Importin N-terminal

Amino acid modifications

Modified residue10801Phosphoserine Ref.10 Ref.11

Secondary structure

.................................................................................................................................................. 1084
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P30822 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: A81833C7F60F5202

FASTA1,084124,104
        10         20         30         40         50         60 
MEGILDFSND LDIALLDQVV STFYQGSGVQ QKQAQEILTK FQDNPDAWQK ADQILQFSTN 

        70         80         90        100        110        120 
PQSKFIALSI LDKLITRKWK LLPNDHRIGI RNFVVGMIIS MCQDDEVFKT QKNLINKSDL 

       130        140        150        160        170        180 
TLVQILKQEW PQNWPEFIPE LIGSSSSSVN VCENNMIVLK LLSEEVFDFS AEQMTQAKAL 

       190        200        210        220        230        240 
HLKNSMSKEF EQIFKLCFQV LEQGSSSSLI VATLESLLRY LHWIPYRYIY ETNILELLST 

       250        260        270        280        290        300 
KFMTSPDTRA ITLKCLTEVS NLKIPQDNDL IKRQTVLFFQ NTLQQIATSV MPVTADLKAT 

       310        320        330        340        350        360 
YANANGNDQS FLQDLAMFLT TYLARNRALL ESDESLRELL LNAHQYLIQL SKIEERELFK 

       370        380        390        400        410        420 
TTLDYWHNLV ADLFYEVQRL PATEMSPLIQ LSVGSQAIST GSGALNPEYM KRFPLKKHIY 

       430        440        450        460        470        480 
EEICSQLRLV IIENMVRPEE VLVVENDEGE IVREFVKESD TIQLYKSERE VLVYLTHLNV 

       490        500        510        520        530        540 
IDTEEIMISK LARQIDGSEW SWHNINTLSW AIGSISGTMS EDTEKRFVVT VIKDLLDLTV 

       550        560        570        580        590        600 
KKRGKDNKAV VASDIMYVVG QYPRFLKAHW NFLRTVILKL FEFMHETHEG VQDMACDTFI 

       610        620        630        640        650        660 
KIVQKCKYHF VIQQPRESEP FIQTIIRDIQ KTTADLQPQQ VHTFYKACGI IISEERSVAE 

       670        680        690        700        710        720 
RNRLLSDLMQ LPNMAWDTIV EQSTANPTLL LDSETVKIIA NIIKTNVAVC TSMGADFYPQ 

       730        740        750        760        770        780 
LGHIYYNMLQ LYRAVSSMIS AQVAAEGLIA TKTPKVRGLR TIKKEILKLV ETYISKARNL 

       790        800        810        820        830        840 
DDVVKVLVEP LLNAVLEDYM NNVPDARDAE VLNCMTTVVE KVGHMIPQGV ILILQSVFEC 

       850        860        870        880        890        900 
TLDMINKDFT EYPEHRVEFY KLLKVINEKS FAAFLELPPA AFKLFVDAIC WAFKHNNRDV 

       910        920        930        940        950        960 
EVNGLQIALD LVKNIERMGN VPFANEFHKN YFFIFVSETF FVLTDSDHKS GFSKQALLLM 

       970        980        990       1000       1010       1020 
KLISLVYDNK ISVPLYQEAE VPQGTSNQVY LSQYLANMLS NAFPHLTSEQ IASFLSALTK 

      1030       1040       1050       1060       1070       1080 
QYKDLVVFKG TLRDFLVQIK EVGGDPTDYL FAEDKENALM EQNRLEREKA AKIGGLLKPS 


ELDD 

« Hide

References

« Hide 'large scale' references
[1]"Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1."
Toda T., Shimanuki M., Saka Y., Yamano H., Adachi Y., Shirakawa M., Kyogoku Y., Yanagida M.
Mol. Cell. Biol. 12:5474-5484(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII."
van der Aart Q.J.M., Kleine K., Steensma H.Y.
Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Exportin 1 (Crm1p) is an essential nuclear export factor."
Stade K., Ford C.S., Guthrie C., Weis K.
Cell 90:1041-1050(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Yeast poly(A)-binding protein Pab1 shuttles between the nucleus and the cytoplasm and functions in mRNA export."
Brune C., Munchel S.E., Fischer N., Podtelejnikov A.V., Weis K.
RNA 11:517-531(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PAB1.
[9]"The yeast splicing factor Prp40p contains functional leucine-rich nuclear export signals that are essential for splicing."
Murphy M.W., Olson B.L., Siliciano P.G.
Genetics 166:53-65(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PRP40.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1080, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1080, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13039 Genomic DNA. Translation: BAA02371.1.
X87941 Genomic DNA. Translation: CAA61166.1.
Z73003 Genomic DNA. Translation: CAA97246.1.
BK006941 Genomic DNA. Translation: DAA08312.1.
PIRS57681.
RefSeqNP_011734.3. NM_001181347.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3M1IX-ray2.00C1-1084[»]
3VYCX-ray2.10A1-1084[»]
4GMXX-ray2.10C1-1058[»]
4GPTX-ray2.22C1-1058[»]
4HATX-ray1.78C1-1058[»]
4HAUX-ray2.00C1-1058[»]
4HAVX-ray2.00C1-1058[»]
4HAWX-ray1.90C1-1058[»]
4HAXX-ray2.28C1-1058[»]
4HAYX-ray2.30C1-1058[»]
4HAZX-ray1.90C1-1058[»]
4HB0X-ray2.20C1-1058[»]
4HB2X-ray1.80C1-1058[»]
4HB3X-ray2.80C1-1058[»]
4HB4X-ray2.05C1-1058[»]
ProteinModelPortalP30822.
SMRP30822. Positions 515-641.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33472. 135 interactions.
DIPDIP-2244N.
IntActP30822. 47 interactions.
MINTMINT-474317.
STRING4932.YGR218W.

Proteomic databases

MaxQBP30822.
PaxDbP30822.
PeptideAtlasP30822.
PRIDEP30822.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR218W; YGR218W; YGR218W.
GeneID853133.
KEGGsce:YGR218W.

Organism-specific databases

CYGDYGR218w.
SGDS000003450. CRM1.

Phylogenomic databases

eggNOGCOG5101.
GeneTreeENSGT00390000000572.
HOGENOMHOG000209012.
KOK14290.
OMASEDTEKR.
OrthoDBEOG7MWH56.

Enzyme and pathway databases

BioCycYEAST:G3O-30900-MONOMER.

Gene expression databases

GenevestigatorP30822.

Family and domain databases

Gene3D1.25.10.10. 4 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR014877. CRM1_C_dom.
IPR013598. Exportin-1/Importin-b-like.
IPR001494. Importin-beta_N.
[Graphical view]
PfamPF08767. CRM1_C. 1 hit.
PF03810. IBN_N. 1 hit.
PF08389. Xpo1. 1 hit.
[Graphical view]
SMARTSM01102. CRM1_C. 1 hit.
SM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 4 hits.
PROSITEPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP30822.
NextBio973192.
PROP30822.

Entry information

Entry nameXPO1_YEAST
AccessionPrimary (citable) accession number: P30822
Secondary accession number(s): D6VV01
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references