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P30822

- XPO1_YEAST

UniProt

P30822 - XPO1_YEAST

Protein

Exportin-1

Gene

CRM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Receptor for the leucine-rich nuclear export signal (NES).

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein transporter activity Source: SGD

    GO - Biological processi

    1. mRNA export from nucleus Source: SGD
    2. protein export from nucleus Source: SGD
    3. protein localization to kinetochore Source: SGD
    4. ribosomal large subunit export from nucleus Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30900-MONOMER.
    ReactomeiREACT_188487. Cyclin A/B1 associated events during G2/M transition.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exportin-1
    Alternative name(s):
    Chromosome region maintenance protein 1
    Karyopherin-124
    Gene namesi
    Name:CRM1
    Synonyms:KAP124, XPO1
    Ordered Locus Names:YGR218W
    ORF Names:G8514
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR218w.
    SGDiS000003450. CRM1.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasmperinuclear region 1 Publication
    Note: Localized in the nucleus and at its periphery.

    GO - Cellular componenti

    1. kinetochore Source: SGD
    2. nucleus Source: SGD
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    4. spindle pole body Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10841084Exportin-1PRO_0000204710Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1080 – 10801Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP30822.
    PaxDbiP30822.
    PeptideAtlasiP30822.
    PRIDEiP30822.

    Expressioni

    Gene expression databases

    GenevestigatoriP30822.

    Interactioni

    Subunit structurei

    Interacts with GSP1/GSP2, polyadenylate-binding protein PAB1 and PRP40.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PKIAP6192517EBI-20589,EBI-2682139From a different organism.

    Protein-protein interaction databases

    BioGridi33472. 135 interactions.
    DIPiDIP-2244N.
    IntActiP30822. 47 interactions.
    MINTiMINT-474317.
    STRINGi4932.YGR218W.

    Structurei

    Secondary structure

    1084
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1 – 55
    Helixi13 – 2513
    Helixi28 – 4316
    Helixi47 – 504
    Helixi51 – 577
    Helixi61 – 7818
    Helixi79 – 813
    Helixi84 – 10320
    Helixi105 – 1106
    Helixi112 – 12918
    Turni130 – 1334
    Helixi137 – 1448
    Turni145 – 1473
    Helixi149 – 16719
    Turni171 – 1733
    Helixi176 – 20328
    Beta strandi205 – 2073
    Helixi208 – 22013
    Turni221 – 2233
    Helixi227 – 2304
    Beta strandi231 – 2333
    Helixi234 – 2396
    Helixi241 – 2444
    Helixi246 – 26015
    Helixi269 – 28921
    Helixi297 – 3037
    Helixi308 – 32619
    Helixi327 – 3304
    Helixi334 – 3363
    Helixi337 – 35014
    Helixi356 – 37318
    Turni414 – 4163
    Helixi417 – 4204
    Helixi421 – 43313
    Beta strandi443 – 4453
    Beta strandi451 – 4533
    Helixi459 – 4613
    Helixi462 – 47817
    Helixi480 – 49516
    Beta strandi497 – 4993
    Helixi502 – 51413
    Turni515 – 5173
    Helixi521 – 54121
    Helixi545 – 56016
    Helixi563 – 5686
    Helixi570 – 58314
    Helixi589 – 60618
    Helixi608 – 6114
    Helixi621 – 6277
    Helixi629 – 6335
    Beta strandi634 – 6363
    Helixi638 – 65215
    Helixi658 – 66811
    Helixi670 – 68516
    Helixi689 – 6913
    Helixi693 – 71321
    Helixi714 – 7174
    Helixi718 – 74629
    Helixi748 – 7525
    Helixi754 – 77623
    Helixi780 – 7867
    Helixi788 – 80114
    Helixi804 – 8063
    Helixi809 – 82214
    Helixi823 – 8253
    Helixi827 – 84519
    Beta strandi849 – 8524
    Helixi853 – 86917
    Helixi872 – 8754
    Helixi879 – 89315
    Helixi898 – 91821
    Helixi922 – 94423
    Beta strandi945 – 9473
    Helixi949 – 9513
    Helixi952 – 96716
    Turni978 – 9803
    Helixi987 – 100216
    Helixi1008 – 102013
    Turni1021 – 10233
    Helixi1025 – 103814
    Beta strandi1041 – 10433
    Helixi1046 – 10505
    Turni1055 – 10573
    Helixi1079 – 10813

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3M1IX-ray2.00C1-1084[»]
    3VYCX-ray2.10A1-1084[»]
    4GMXX-ray2.10C1-1058[»]
    4GPTX-ray2.22C1-1058[»]
    4HATX-ray1.78C1-1058[»]
    4HAUX-ray2.00C1-1058[»]
    4HAVX-ray2.00C1-1058[»]
    4HAWX-ray1.90C1-1058[»]
    4HAXX-ray2.28C1-1058[»]
    4HAYX-ray2.30C1-1058[»]
    4HAZX-ray1.90C1-1058[»]
    4HB0X-ray2.20C1-1058[»]
    4HB2X-ray1.80C1-1058[»]
    4HB3X-ray2.80C1-1058[»]
    4HB4X-ray2.05C1-1058[»]
    ProteinModelPortaliP30822.
    SMRiP30822. Positions 515-641.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP30822.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 10067Importin N-terminalPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the exportin family.Curated
    Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5101.
    GeneTreeiENSGT00390000000572.
    HOGENOMiHOG000209012.
    KOiK14290.
    OMAiSEDTEKR.
    OrthoDBiEOG7MWH56.

    Family and domain databases

    Gene3Di1.25.10.10. 4 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR014877. CRM1_C_dom.
    IPR013598. Exportin-1/Importin-b-like.
    IPR001494. Importin-beta_N.
    [Graphical view]
    PfamiPF08767. CRM1_C. 1 hit.
    PF03810. IBN_N. 1 hit.
    PF08389. Xpo1. 1 hit.
    [Graphical view]
    SMARTiSM01102. CRM1_C. 1 hit.
    SM00913. IBN_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 4 hits.
    PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30822-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGILDFSND LDIALLDQVV STFYQGSGVQ QKQAQEILTK FQDNPDAWQK     50
    ADQILQFSTN PQSKFIALSI LDKLITRKWK LLPNDHRIGI RNFVVGMIIS 100
    MCQDDEVFKT QKNLINKSDL TLVQILKQEW PQNWPEFIPE LIGSSSSSVN 150
    VCENNMIVLK LLSEEVFDFS AEQMTQAKAL HLKNSMSKEF EQIFKLCFQV 200
    LEQGSSSSLI VATLESLLRY LHWIPYRYIY ETNILELLST KFMTSPDTRA 250
    ITLKCLTEVS NLKIPQDNDL IKRQTVLFFQ NTLQQIATSV MPVTADLKAT 300
    YANANGNDQS FLQDLAMFLT TYLARNRALL ESDESLRELL LNAHQYLIQL 350
    SKIEERELFK TTLDYWHNLV ADLFYEVQRL PATEMSPLIQ LSVGSQAIST 400
    GSGALNPEYM KRFPLKKHIY EEICSQLRLV IIENMVRPEE VLVVENDEGE 450
    IVREFVKESD TIQLYKSERE VLVYLTHLNV IDTEEIMISK LARQIDGSEW 500
    SWHNINTLSW AIGSISGTMS EDTEKRFVVT VIKDLLDLTV KKRGKDNKAV 550
    VASDIMYVVG QYPRFLKAHW NFLRTVILKL FEFMHETHEG VQDMACDTFI 600
    KIVQKCKYHF VIQQPRESEP FIQTIIRDIQ KTTADLQPQQ VHTFYKACGI 650
    IISEERSVAE RNRLLSDLMQ LPNMAWDTIV EQSTANPTLL LDSETVKIIA 700
    NIIKTNVAVC TSMGADFYPQ LGHIYYNMLQ LYRAVSSMIS AQVAAEGLIA 750
    TKTPKVRGLR TIKKEILKLV ETYISKARNL DDVVKVLVEP LLNAVLEDYM 800
    NNVPDARDAE VLNCMTTVVE KVGHMIPQGV ILILQSVFEC TLDMINKDFT 850
    EYPEHRVEFY KLLKVINEKS FAAFLELPPA AFKLFVDAIC WAFKHNNRDV 900
    EVNGLQIALD LVKNIERMGN VPFANEFHKN YFFIFVSETF FVLTDSDHKS 950
    GFSKQALLLM KLISLVYDNK ISVPLYQEAE VPQGTSNQVY LSQYLANMLS 1000
    NAFPHLTSEQ IASFLSALTK QYKDLVVFKG TLRDFLVQIK EVGGDPTDYL 1050
    FAEDKENALM EQNRLEREKA AKIGGLLKPS ELDD 1084
    Length:1,084
    Mass (Da):124,104
    Last modified:July 1, 1993 - v1
    Checksum:iA81833C7F60F5202
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13039 Genomic DNA. Translation: BAA02371.1.
    X87941 Genomic DNA. Translation: CAA61166.1.
    Z73003 Genomic DNA. Translation: CAA97246.1.
    BK006941 Genomic DNA. Translation: DAA08312.1.
    PIRiS57681.
    RefSeqiNP_011734.3. NM_001181347.3.

    Genome annotation databases

    EnsemblFungiiYGR218W; YGR218W; YGR218W.
    GeneIDi853133.
    KEGGisce:YGR218W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13039 Genomic DNA. Translation: BAA02371.1 .
    X87941 Genomic DNA. Translation: CAA61166.1 .
    Z73003 Genomic DNA. Translation: CAA97246.1 .
    BK006941 Genomic DNA. Translation: DAA08312.1 .
    PIRi S57681.
    RefSeqi NP_011734.3. NM_001181347.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3M1I X-ray 2.00 C 1-1084 [» ]
    3VYC X-ray 2.10 A 1-1084 [» ]
    4GMX X-ray 2.10 C 1-1058 [» ]
    4GPT X-ray 2.22 C 1-1058 [» ]
    4HAT X-ray 1.78 C 1-1058 [» ]
    4HAU X-ray 2.00 C 1-1058 [» ]
    4HAV X-ray 2.00 C 1-1058 [» ]
    4HAW X-ray 1.90 C 1-1058 [» ]
    4HAX X-ray 2.28 C 1-1058 [» ]
    4HAY X-ray 2.30 C 1-1058 [» ]
    4HAZ X-ray 1.90 C 1-1058 [» ]
    4HB0 X-ray 2.20 C 1-1058 [» ]
    4HB2 X-ray 1.80 C 1-1058 [» ]
    4HB3 X-ray 2.80 C 1-1058 [» ]
    4HB4 X-ray 2.05 C 1-1058 [» ]
    ProteinModelPortali P30822.
    SMRi P30822. Positions 515-641.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33472. 135 interactions.
    DIPi DIP-2244N.
    IntActi P30822. 47 interactions.
    MINTi MINT-474317.
    STRINGi 4932.YGR218W.

    Proteomic databases

    MaxQBi P30822.
    PaxDbi P30822.
    PeptideAtlasi P30822.
    PRIDEi P30822.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR218W ; YGR218W ; YGR218W .
    GeneIDi 853133.
    KEGGi sce:YGR218W.

    Organism-specific databases

    CYGDi YGR218w.
    SGDi S000003450. CRM1.

    Phylogenomic databases

    eggNOGi COG5101.
    GeneTreei ENSGT00390000000572.
    HOGENOMi HOG000209012.
    KOi K14290.
    OMAi SEDTEKR.
    OrthoDBi EOG7MWH56.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30900-MONOMER.
    Reactomei REACT_188487. Cyclin A/B1 associated events during G2/M transition.

    Miscellaneous databases

    EvolutionaryTracei P30822.
    NextBioi 973192.
    PROi P30822.

    Gene expression databases

    Genevestigatori P30822.

    Family and domain databases

    Gene3Di 1.25.10.10. 4 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR014877. CRM1_C_dom.
    IPR013598. Exportin-1/Importin-b-like.
    IPR001494. Importin-beta_N.
    [Graphical view ]
    Pfami PF08767. CRM1_C. 1 hit.
    PF03810. IBN_N. 1 hit.
    PF08389. Xpo1. 1 hit.
    [Graphical view ]
    SMARTi SM01102. CRM1_C. 1 hit.
    SM00913. IBN_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 4 hits.
    PROSITEi PS50166. IMPORTIN_B_NT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1."
      Toda T., Shimanuki M., Saka Y., Yamano H., Adachi Y., Shirakawa M., Kyogoku Y., Yanagida M.
      Mol. Cell. Biol. 12:5474-5484(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII."
      van der Aart Q.J.M., Kleine K., Steensma H.Y.
      Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Exportin 1 (Crm1p) is an essential nuclear export factor."
      Stade K., Ford C.S., Guthrie C., Weis K.
      Cell 90:1041-1050(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Yeast poly(A)-binding protein Pab1 shuttles between the nucleus and the cytoplasm and functions in mRNA export."
      Brune C., Munchel S.E., Fischer N., Podtelejnikov A.V., Weis K.
      RNA 11:517-531(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PAB1.
    9. "The yeast splicing factor Prp40p contains functional leucine-rich nuclear export signals that are essential for splicing."
      Murphy M.W., Olson B.L., Siliciano P.G.
      Genetics 166:53-65(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRP40.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1080, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1080, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiXPO1_YEAST
    AccessioniPrimary (citable) accession number: P30822
    Secondary accession number(s): D6VV01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7080 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3