P30799 (DDH_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-hydroxyacid dehydrogenase homolog
EC=1.1.1.-

Gene names

Name:	ddh
Synonyms:	ldhA
Ordered Locus Names:	ZMO1237

Organism

Zymomonas mobilis [Complete proteome] [HAMAP]

Taxonomic identifier

542 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	2-hydroxyacid dehydrogenase homolog		PRO_0000076020
Regions
	Nucleotide binding	154 – 155	2	NAD By similarity
	Nucleotide binding	234 – 236	3	NAD By similarity
	Nucleotide binding	297 – 300	4	NAD By similarity
Sites
	Active site	236	1	By similarity
	Active site	265	1	By similarity
	Active site	297	1	Proton donor By similarity
	Binding site	260	1	NAD By similarity
Experimental info
	Sequence conflict	184	1	K → E in AAA71934. Ref.1
	Sequence conflict	219	1	E → G in AAA71934. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P30799 [UniParc]. Last modified February 15, 2005. Version 3. Checksum: CFEBF136F9FCC4C4	FASTA	331	36,496
	10 20 30 40 50 60 MRVAIFSSKN YDHHSIEKEN EHYGHDLVFL NERLTKETAE KAKDAEAVCI FVNDEANAEV 70 80 90 100 110 120 LEILAGLGIK LVALRCAGYN NVDLDAAKKL NIKVVRVPAY SPYSVAEYAV GMLLTLNRQI 130 140 150 160 170 180 SRGLKRVREN NFSLEGLIGL DVHDKTVGII GVGHIGSVFA HIMTHGFGAN VIAYKPHPDP 190 200 210 220 230 240 ELAKKVGFRF TSLDEVIETS DIISLHCPLT PENHHMINEE TLARAKKGFY LVNTSRGGLV 250 260 270 280 290 300 DTKAVIKSLK AKHLGGYAAD VYEEEGPLFF ENHADDIIED DILERLIAFP NVVFTGHQAF 310 320 330 LTKEALSNIA HSILQDISDA EAGKEMPDAL V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli." Yomano L.P., Scopes R.K., Ingram L.O. J. Bacteriol. 175:3926-3933(1993) [PubMed: 8320209] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.
[2]	"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L09650 Genomic DNA. Translation: AAA71934.1. L09651 Unassigned DNA. Translation: AAA71938.1. AE008692 Genomic DNA. Translation: AAV89861.1.
PIR	D40649.
RefSeq	YP_162972.1. NC_006526.2.
3D structure databases
ProteinModelPortal	P30799.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3188392.
GenomeReviews	Gene locus ZMO1237 in contig AE008692_GR.
KEGG	zmo:ZMO1237.
NMPDR	fig|264203.3.peg.429.
PATRIC	32567770. VBIZymMob102260_1173.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG731446.
OMA	LEFFDFM.
ProtClustDB	CLSK2524817.
Enzyme and pathway databases
BioCyc	ZMOB264203:ZMO1237-MONOMER.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KO	K03778.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDH_ZYMMO

Accession

Primary (citable) accession number: P30799
Secondary accession number(s): Q5NN49

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program