Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P30775

- RF1M_YEAST

UniProt

P30775 - RF1M_YEAST

Protein

Peptide chain release factor 1, mitochondrial

Gene

MRF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. translation release factor activity Source: SGD
    3. translation release factor activity, codon specific Source: InterPro

    GO - Biological processi

    1. mitochondrial translation Source: SGD
    2. mitochondrial translational termination Source: SGD
    3. translational termination Source: SGD

    Keywords - Biological processi

    Protein biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30637-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptide chain release factor 1, mitochondrial
    Short name:
    MRF-1
    Short name:
    MtRF-1
    Gene namesi
    Name:MRF1
    Ordered Locus Names:YGL143C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL143c.
    SGDiS000003111. MRF1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 413Peptide chain release factor 1, mitochondrialPRO_0000030337
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei287 – 2871N5-methylglutamine1 Publication

    Post-translational modificationi

    Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals By similarity. N5-methylated on Gln-287 by MTQ1.By similarity1 Publication

    Keywords - PTMi

    Methylation

    Proteomic databases

    MaxQBiP30775.
    PaxDbiP30775.

    Expressioni

    Gene expression databases

    GenevestigatoriP30775.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MTQ1P539442EBI-14964,EBI-28737

    Protein-protein interaction databases

    BioGridi33109. 12 interactions.
    DIPiDIP-2626N.
    IntActiP30775. 2 interactions.
    MINTiMINT-427494.
    STRINGi4932.YGL143C.

    Structurei

    3D structure databases

    ProteinModelPortaliP30775.
    SMRiP30775. Positions 83-411.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0216.
    GeneTreeiENSGT00390000011879.
    HOGENOMiHOG000074815.
    KOiK02835.
    OMAiYERTFKP.
    OrthoDBiEOG789CQG.

    Family and domain databases

    Gene3Di3.30.160.20. 1 hit.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR005139. PCRF.
    IPR000352. Pep_chain_release_fac_I_II.
    [Graphical view]
    PfamiPF03462. PCRF. 1 hit.
    PF00472. RF-1. 1 hit.
    [Graphical view]
    SMARTiSM00937. PCRF. 1 hit.
    [Graphical view]
    PROSITEiPS00745. RF_PROK_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30775-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWLSKFQFPS RSIFKGVFLG HKLPLLVRLT STTTNSKSNG SIPTQYTELS    50
    PLLVKQAEKY EAELKDLDKD LSCGIHFDVN KQKHYAKLSA LTDTFIEYKE 100
    KLNELKSLQE MIVSDPSLRA EAEQEYAELV PQYETTSSRL VNKLLPPHPF 150
    ADKPSLLELR PGVGGIEAMI FTQNLLDMYI GYANYRKWKY RIISKNENES 200
    GSGIIDAILS IEEAGSYDRL RFEAGVHRVQ RIPSTETKGR THTSTAAVVV 250
    LPQIGDESAK SIDAYERTFK PGEIRVDIMR ASGKGGQHVN TTDSAVRLTH 300
    IPSGIVVSMQ DERSQHKNKA KAFTILRARL AEKERLEKEE KERKARKSQV 350
    SSTNRSDKIR TYNFPQNRIT DHRCGFTLLD LPGVLSGERL DEVIEAMSKY 400
    DSTERAKELL ESN 413
    Length:413
    Mass (Da):46,770
    Last modified:July 1, 1993 - v1
    Checksum:iAAAD4829748C7604
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60381 Genomic DNA. Translation: CAA42932.1.
    X99960 Genomic DNA. Translation: CAA68219.1.
    Z72665 Genomic DNA. Translation: CAA96855.1.
    BK006941 Genomic DNA. Translation: DAA07967.1.
    PIRiS28602.
    RefSeqiNP_011372.3. NM_001181008.3.

    Genome annotation databases

    EnsemblFungiiYGL143C; YGL143C; YGL143C.
    GeneIDi852734.
    KEGGisce:YGL143C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60381 Genomic DNA. Translation: CAA42932.1 .
    X99960 Genomic DNA. Translation: CAA68219.1 .
    Z72665 Genomic DNA. Translation: CAA96855.1 .
    BK006941 Genomic DNA. Translation: DAA07967.1 .
    PIRi S28602.
    RefSeqi NP_011372.3. NM_001181008.3.

    3D structure databases

    ProteinModelPortali P30775.
    SMRi P30775. Positions 83-411.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33109. 12 interactions.
    DIPi DIP-2626N.
    IntActi P30775. 2 interactions.
    MINTi MINT-427494.
    STRINGi 4932.YGL143C.

    Proteomic databases

    MaxQBi P30775.
    PaxDbi P30775.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL143C ; YGL143C ; YGL143C .
    GeneIDi 852734.
    KEGGi sce:YGL143C.

    Organism-specific databases

    CYGDi YGL143c.
    SGDi S000003111. MRF1.

    Phylogenomic databases

    eggNOGi COG0216.
    GeneTreei ENSGT00390000011879.
    HOGENOMi HOG000074815.
    KOi K02835.
    OMAi YERTFKP.
    OrthoDBi EOG789CQG.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30637-MONOMER.

    Miscellaneous databases

    NextBioi 972135.
    PROi P30775.

    Gene expression databases

    Genevestigatori P30775.

    Family and domain databases

    Gene3Di 3.30.160.20. 1 hit.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR005139. PCRF.
    IPR000352. Pep_chain_release_fac_I_II.
    [Graphical view ]
    Pfami PF03462. PCRF. 1 hit.
    PF00472. RF-1. 1 hit.
    [Graphical view ]
    SMARTi SM00937. PCRF. 1 hit.
    [Graphical view ]
    PROSITEi PS00745. RF_PROK_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast nuclear gene MRF1 encodes a mitochondrial peptide chain release factor and cures several mitochondrial RNA splicing defects."
      Pel H.J., Maat M.J., Rep M., Grivell L.A.
      Nucleic Acids Res. 20:6339-6346(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames."
      Voet M., Defoor E., Verhasselt P., Riles L., Robben J., Volckaert G.
      Yeast 13:177-182(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p."
      Polevoda B., Span L., Sherman F.
      J. Biol. Chem. 281:2562-2571(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT GLN-287, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiRF1M_YEAST
    AccessioniPrimary (citable) accession number: P30775
    Secondary accession number(s): D6VU06
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 414 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3