Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P30775 (RF1M_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide chain release factor 1, mitochondrial

Short name=MRF-1
Short name=MtRF-1
Gene names
Name:MRF1
Ordered Locus Names:YGL143C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length413 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG.

Subcellular location

Mitochondrion.

Post-translational modification

Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals By similarity. N5-methylated on Gln-287 by MTQ1. Ref.6

Miscellaneous

Present with 414 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the prokaryotic/mitochondrial release factor family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MTQ1P539442EBI-14964,EBI-28737

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 413Peptide chain release factor 1, mitochondrialPRO_0000030337

Amino acid modifications

Modified residue2871N5-methylglutamine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P30775 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: AAAD4829748C7604

FASTA41346,770
        10         20         30         40         50         60 
MWLSKFQFPS RSIFKGVFLG HKLPLLVRLT STTTNSKSNG SIPTQYTELS PLLVKQAEKY 

        70         80         90        100        110        120 
EAELKDLDKD LSCGIHFDVN KQKHYAKLSA LTDTFIEYKE KLNELKSLQE MIVSDPSLRA 

       130        140        150        160        170        180 
EAEQEYAELV PQYETTSSRL VNKLLPPHPF ADKPSLLELR PGVGGIEAMI FTQNLLDMYI 

       190        200        210        220        230        240 
GYANYRKWKY RIISKNENES GSGIIDAILS IEEAGSYDRL RFEAGVHRVQ RIPSTETKGR 

       250        260        270        280        290        300 
THTSTAAVVV LPQIGDESAK SIDAYERTFK PGEIRVDIMR ASGKGGQHVN TTDSAVRLTH 

       310        320        330        340        350        360 
IPSGIVVSMQ DERSQHKNKA KAFTILRARL AEKERLEKEE KERKARKSQV SSTNRSDKIR 

       370        380        390        400        410 
TYNFPQNRIT DHRCGFTLLD LPGVLSGERL DEVIEAMSKY DSTERAKELL ESN 

« Hide

References

« Hide 'large scale' references
[1]"The yeast nuclear gene MRF1 encodes a mitochondrial peptide chain release factor and cures several mitochondrial RNA splicing defects."
Pel H.J., Maat M.J., Rep M., Grivell L.A.
Nucleic Acids Res. 20:6339-6346(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames."
Voet M., Defoor E., Verhasselt P., Riles L., Robben J., Volckaert G.
Yeast 13:177-182(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p."
Polevoda B., Span L., Sherman F.
J. Biol. Chem. 281:2562-2571(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT GLN-287, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60381 Genomic DNA. Translation: CAA42932.1.
X99960 Genomic DNA. Translation: CAA68219.1.
Z72665 Genomic DNA. Translation: CAA96855.1.
BK006941 Genomic DNA. Translation: DAA07967.1.
PIRS28602.
RefSeqNP_011372.3. NM_001181008.3.

3D structure databases

ProteinModelPortalP30775.
SMRP30775. Positions 54-411.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33109. 12 interactions.
DIPDIP-2626N.
IntActP30775. 2 interactions.
MINTMINT-427494.
STRING4932.YGL143C.

Proteomic databases

PaxDbP30775.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL143C; YGL143C; YGL143C.
GeneID852734.
KEGGsce:YGL143C.

Organism-specific databases

CYGDYGL143c.
SGDS000003111. MRF1.

Phylogenomic databases

eggNOGCOG0216.
GeneTreeENSGT00390000011879.
HOGENOMHOG000074815.
KOK02835.
OMAPFAHLPC.
OrthoDBEOG789CQG.

Enzyme and pathway databases

BioCycYEAST:G3O-30637-MONOMER.

Gene expression databases

GenevestigatorP30775.

Family and domain databases

Gene3D3.30.160.20. 1 hit.
InterProIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
[Graphical view]
PfamPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTSM00937. PCRF. 1 hit.
[Graphical view]
PROSITEPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972135.
PROP30775.

Entry information

Entry nameRF1M_YEAST
AccessionPrimary (citable) accession number: P30775
Secondary accession number(s): D6VU06
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families