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Protein

Peptide chain release factor 1, mitochondrial

Gene

MRF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG.

GO - Molecular functioni

GO - Biological processi

  • mitochondrial translation Source: SGD
  • mitochondrial translational termination Source: SGD
  • translational termination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-30637-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide chain release factor 1, mitochondrial
Short name:
MRF-1
Short name:
MtRF-1
Gene namesi
Name:MRF1
Ordered Locus Names:YGL143C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGL143c.
EuPathDBiFungiDB:YGL143C.
SGDiS000003111. MRF1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 413Peptide chain release factor 1, mitochondrialPRO_0000030337
Transit peptidei1 – ?MitochondrionSequence Analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei287 – 2871N5-methylglutamine1 Publication

Post-translational modificationi

Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals (By similarity). N5-methylated on Gln-287 by MTQ1.By similarity1 Publication

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP30775.
PaxDbiP30775.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MTQ1P539442EBI-14964,EBI-28737

Protein-protein interaction databases

BioGridi33109. 12 interactions.
DIPiDIP-2626N.
IntActiP30775. 2 interactions.
MINTiMINT-427494.

Structurei

3D structure databases

ProteinModelPortaliP30775.
SMRiP30775. Positions 97-410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0216.
GeneTreeiENSGT00390000011879.
HOGENOMiHOG000074815.
InParanoidiP30775.
KOiK02835.
OMAiYERTFKP.
OrthoDBiEOG789CQG.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
[Graphical view]
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30775-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLSKFQFPS RSIFKGVFLG HKLPLLVRLT STTTNSKSNG SIPTQYTELS
60 70 80 90 100
PLLVKQAEKY EAELKDLDKD LSCGIHFDVN KQKHYAKLSA LTDTFIEYKE
110 120 130 140 150
KLNELKSLQE MIVSDPSLRA EAEQEYAELV PQYETTSSRL VNKLLPPHPF
160 170 180 190 200
ADKPSLLELR PGVGGIEAMI FTQNLLDMYI GYANYRKWKY RIISKNENES
210 220 230 240 250
GSGIIDAILS IEEAGSYDRL RFEAGVHRVQ RIPSTETKGR THTSTAAVVV
260 270 280 290 300
LPQIGDESAK SIDAYERTFK PGEIRVDIMR ASGKGGQHVN TTDSAVRLTH
310 320 330 340 350
IPSGIVVSMQ DERSQHKNKA KAFTILRARL AEKERLEKEE KERKARKSQV
360 370 380 390 400
SSTNRSDKIR TYNFPQNRIT DHRCGFTLLD LPGVLSGERL DEVIEAMSKY
410
DSTERAKELL ESN
Length:413
Mass (Da):46,770
Last modified:July 1, 1993 - v1
Checksum:iAAAD4829748C7604
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60381 Genomic DNA. Translation: CAA42932.1.
X99960 Genomic DNA. Translation: CAA68219.1.
Z72665 Genomic DNA. Translation: CAA96855.1.
BK006941 Genomic DNA. Translation: DAA07967.1.
PIRiS28602.
RefSeqiNP_011372.3. NM_001181008.3.

Genome annotation databases

EnsemblFungiiYGL143C; YGL143C; YGL143C.
GeneIDi852734.
KEGGisce:YGL143C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60381 Genomic DNA. Translation: CAA42932.1.
X99960 Genomic DNA. Translation: CAA68219.1.
Z72665 Genomic DNA. Translation: CAA96855.1.
BK006941 Genomic DNA. Translation: DAA07967.1.
PIRiS28602.
RefSeqiNP_011372.3. NM_001181008.3.

3D structure databases

ProteinModelPortaliP30775.
SMRiP30775. Positions 97-410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33109. 12 interactions.
DIPiDIP-2626N.
IntActiP30775. 2 interactions.
MINTiMINT-427494.

Proteomic databases

MaxQBiP30775.
PaxDbiP30775.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL143C; YGL143C; YGL143C.
GeneIDi852734.
KEGGisce:YGL143C.

Organism-specific databases

CYGDiYGL143c.
EuPathDBiFungiDB:YGL143C.
SGDiS000003111. MRF1.

Phylogenomic databases

eggNOGiCOG0216.
GeneTreeiENSGT00390000011879.
HOGENOMiHOG000074815.
InParanoidiP30775.
KOiK02835.
OMAiYERTFKP.
OrthoDBiEOG789CQG.

Enzyme and pathway databases

BioCyciYEAST:G3O-30637-MONOMER.

Miscellaneous databases

NextBioi972135.
PROiP30775.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
[Graphical view]
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast nuclear gene MRF1 encodes a mitochondrial peptide chain release factor and cures several mitochondrial RNA splicing defects."
    Pel H.J., Maat M.J., Rep M., Grivell L.A.
    Nucleic Acids Res. 20:6339-6346(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames."
    Voet M., Defoor E., Verhasselt P., Riles L., Robben J., Volckaert G.
    Yeast 13:177-182(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p."
    Polevoda B., Span L., Sherman F.
    J. Biol. Chem. 281:2562-2571(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT GLN-287, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiRF1M_YEAST
AccessioniPrimary (citable) accession number: P30775
Secondary accession number(s): D6VU06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 22, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 414 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.