P30775 (RF1M_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide chain release factor 1, mitochondrial
Short name=MRF-1
Short name=MtRF-1

Gene names

Name:	MRF1
Ordered Locus Names:	YGL143C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG.
Subcellular location	Mitochondrion.
Post-translational modification	Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals By similarity. N5-methylated on Gln-287 by MTQ1. Ref.6
Miscellaneous	Present with 414 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial translational termination Inferred from mutant phenotype PubMed 15827612. Source: SGD
Cellular_component	mitochondrion Inferred from direct assay PubMed 11502169PubMed 14576278PubMed 16823961PubMed 8265342. Source: SGD
Molecular_function	translation release factor activity Inferred from direct assay PubMed 10748224. Source: SGD translation release factor activity, codon specific Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MTQ1	P53944	2	EBI-14964,EBI-28737

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 413		Peptide chain release factor 1, mitochondrial		PRO_0000030337
Amino acid modifications
	Modified residue	287	1	N5-methylglutamine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P30775 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: AAAD4829748C7604	FASTA	413	46,770
	10 20 30 40 50 60 MWLSKFQFPS RSIFKGVFLG HKLPLLVRLT STTTNSKSNG SIPTQYTELS PLLVKQAEKY 70 80 90 100 110 120 EAELKDLDKD LSCGIHFDVN KQKHYAKLSA LTDTFIEYKE KLNELKSLQE MIVSDPSLRA 130 140 150 160 170 180 EAEQEYAELV PQYETTSSRL VNKLLPPHPF ADKPSLLELR PGVGGIEAMI FTQNLLDMYI 190 200 210 220 230 240 GYANYRKWKY RIISKNENES GSGIIDAILS IEEAGSYDRL RFEAGVHRVQ RIPSTETKGR 250 260 270 280 290 300 THTSTAAVVV LPQIGDESAK SIDAYERTFK PGEIRVDIMR ASGKGGQHVN TTDSAVRLTH 310 320 330 340 350 360 IPSGIVVSMQ DERSQHKNKA KAFTILRARL AEKERLEKEE KERKARKSQV SSTNRSDKIR 370 380 390 400 410 TYNFPQNRIT DHRCGFTLLD LPGVLSGERL DEVIEAMSKY DSTERAKELL ESN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast nuclear gene MRF1 encodes a mitochondrial peptide chain release factor and cures several mitochondrial RNA splicing defects." Pel H.J., Maat M.J., Rep M., Grivell L.A. Nucleic Acids Res. 20:6339-6346(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames." Voet M., Defoor E., Verhasselt P., Riles L., Robben J., Volckaert G. Yeast 13:177-182(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p." Polevoda B., Span L., Sherman F. J. Biol. Chem. 281:2562-2571(2006) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT GLN-287, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60381 Genomic DNA. Translation: CAA42932.1. X99960 Genomic DNA. Translation: CAA68219.1. Z72665 Genomic DNA. Translation: CAA96855.1. BK006941 Genomic DNA. Translation: DAA07967.1.
PIR	S28602.
RefSeq	NP_011372.3. NM_001181008.3.
3D structure databases
ProteinModelPortal	P30775.
SMR	P30775. Positions 83-411.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2626N.
IntAct	P30775. 1 interaction.
MINT	MINT-427494.
STRING	4932.YGL143C.
Proteomic databases
PaxDb	P30775.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL143C; YGL143C; YGL143C.
GeneID	852734.
KEGG	sce:YGL139W. sce:YGL143C.
Organism-specific databases
CYGD	YGL143c.
SGD	S000003111. MRF1.
Phylogenomic databases
eggNOG	COG0216.
GeneTree	ENSGT00390000011879.
HOGENOM	HOG000074815.
KO	K02835.
OMA	PPHPFAD.
OrthoDB	EOG4JWZP5.
Gene expression databases
Genevestigator	P30775.
GermOnline	YGL143C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.160.20. 1 hit.
InterPro	IPR014720. dsRNA-bd-like_dom. IPR005139. PCRF. IPR000352. Pep_chain_release_fac_I_II. [Graphical view]
Pfam	PF03462. PCRF. 1 hit. PF00472. RF-1. 1 hit. [Graphical view]
SMART	SM00937. PCRF. 1 hit. [Graphical view]
PROSITE	PS00745. RF_PROK_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972135.

Entry information

Entry name

RF1M_YEAST

Accession

Primary (citable) accession number: P30775
Secondary accession number(s): D6VU06

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program