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Protein

Peptide chain release factor 1, mitochondrial

Gene

MRF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG.

Miscellaneous

Present with 414 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • translation release factor activity Source: SGD

GO - Biological processi

  • mitochondrial translation Source: SGD
  • mitochondrial translational termination Source: SGD
  • translational termination Source: SGD

Keywordsi

Biological processProtein biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-30637-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide chain release factor 1, mitochondrial
Short name:
MRF-1
Short name:
MtRF-1
Gene namesi
Name:MRF1
Ordered Locus Names:YGL143C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL143C
SGDiS000003111 MRF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000030337? – 413Peptide chain release factor 1, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei287N5-methylglutamine1 Publication1

Post-translational modificationi

Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals (By similarity). N5-methylated on Gln-287 by MTQ1.By similarity1 Publication

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP30775
PaxDbiP30775
PRIDEiP30775

PTM databases

iPTMnetiP30775

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MTQ1P539442EBI-14964,EBI-28737

Protein-protein interaction databases

BioGridi33109, 95 interactors
DIPiDIP-2626N
IntActiP30775, 2 interactors
MINTiP30775
STRINGi4932.YGL143C

Structurei

3D structure databases

ProteinModelPortaliP30775
SMRiP30775
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000011879
HOGENOMiHOG000074815
InParanoidiP30775
KOiK02835
OMAiWKVEIVS
OrthoDBiEOG092C4A6U

Family and domain databases

InterProiView protein in InterPro
IPR005139 PCRF
IPR000352 Pep_chain_release_fac_I_II
PfamiView protein in Pfam
PF03462 PCRF, 1 hit
PF00472 RF-1, 1 hit
SMARTiView protein in SMART
SM00937 PCRF, 1 hit
PROSITEiView protein in PROSITE
PS00745 RF_PROK_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30775-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLSKFQFPS RSIFKGVFLG HKLPLLVRLT STTTNSKSNG SIPTQYTELS
60 70 80 90 100
PLLVKQAEKY EAELKDLDKD LSCGIHFDVN KQKHYAKLSA LTDTFIEYKE
110 120 130 140 150
KLNELKSLQE MIVSDPSLRA EAEQEYAELV PQYETTSSRL VNKLLPPHPF
160 170 180 190 200
ADKPSLLELR PGVGGIEAMI FTQNLLDMYI GYANYRKWKY RIISKNENES
210 220 230 240 250
GSGIIDAILS IEEAGSYDRL RFEAGVHRVQ RIPSTETKGR THTSTAAVVV
260 270 280 290 300
LPQIGDESAK SIDAYERTFK PGEIRVDIMR ASGKGGQHVN TTDSAVRLTH
310 320 330 340 350
IPSGIVVSMQ DERSQHKNKA KAFTILRARL AEKERLEKEE KERKARKSQV
360 370 380 390 400
SSTNRSDKIR TYNFPQNRIT DHRCGFTLLD LPGVLSGERL DEVIEAMSKY
410
DSTERAKELL ESN
Length:413
Mass (Da):46,770
Last modified:July 1, 1993 - v1
Checksum:iAAAD4829748C7604
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60381 Genomic DNA Translation: CAA42932.1
X99960 Genomic DNA Translation: CAA68219.1
Z72665 Genomic DNA Translation: CAA96855.1
BK006941 Genomic DNA Translation: DAA07967.1
PIRiS28602
RefSeqiNP_011372.3, NM_001181008.3

Genome annotation databases

EnsemblFungiiYGL143C; YGL143C; YGL143C
GeneIDi852734
KEGGisce:YGL143C

Similar proteinsi

Entry informationi

Entry nameiRF1M_YEAST
AccessioniPrimary (citable) accession number: P30775
Secondary accession number(s): D6VU06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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