Reviewed, UniProtKB/Swiss-Prot P30774 (MTX1_XANCC)

Names and origin

Protein names

Recommended name:
    Modification methylase XcyI
      Short name=M.XcyI
    EC=2.1.1.113
Alternative name(s):
    N-4 cytosine-specific methyltransferase XcyI

Gene names

Name:

xcyIM

Organism

Xanthomonas campestris pv. cyanopsidis

Taxonomic identifier

29444 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCCGGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the XcyI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Modification methylase XcyI		PRO_0000087935

Sequences

	Sequence		Length	Mass (Da)
	P30774-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 4DB6B942C79484E9	FASTA	300	33,618
	10 20 30 40 50 60 MPKANTAPPS GVERILRPEP LILRGSTLFE GDALTVLRRL PSGSVRCVVT SPPYWGLRDY 70 80 90 100 110 120 GIEEQIGLEV TMPQFLHRLV AIFAEVKRVL TDDGTLWLNI GDGYTSGNRG YRAPDKKNPA 130 140 150 160 170 180 RAMDVRPDTP VGLKPKDLMG IPWRLAFALQ DDGWYLRSDI VWNKPNAMPE SVKDRPARSH 190 200 210 220 230 240 EFLFMFTKSE KYFYDWQAAR EPADGGGLRN RRSVWNVNTK PFAGAHFTTF PPELIRPCIH 250 260 270 280 290 300 ASTEPGDYVL DPFFGSGTVG LVCQDENRQY VGIELNPEYV TLAADRLQGQ NSNVIRIAAA

References

[1]	"Structure and evolution of the XcyI restriction-modification system." Withers B., Ambroso L.A., Dunbar J.C. Nucleic Acids Res. 20:6267-6273(1992) [PubMed: 1475187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 13D5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M98768 Genomic DNA. Translation: AAA27608.1.
PIR	S35549.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Protein family/group databases
REBASE	3529. M.XcyI.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR001091. MeTrfase_CN4. IPR017985. MeTrfase_CN4_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTX1_XANCC

Accession

Primary (citable) accession number: P30774

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)