P30773 (T2X1_XANCC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme XcyI
Short name=R.XcyI
EC=3.1.21.4

Alternative name(s):
Endonuclease XcyI
Type II restriction enzyme XcyI

Gene names

Name:

xcyIR

Organism

Xanthomonas campestris pv. cyanopsidis

Taxonomic identifier

29444 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas › Xanthomonas campestris

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence CCCGGG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium.
Subunit structure	Monomer.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Type-2 restriction enzyme XcyI		PRO_0000077371

Sequences

	Sequence		Length	Mass (Da)
	P30773 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: DA0DCBB8CCDBD55A	FASTA	333	36,686
	10 20 30 40 50 60 MTTPNKINFP PPKLQIDFAF ALKRFRAVYL QSALLETVRD MDIAELDRQL SKYVPPADLA 70 80 90 100 110 120 TLAQYGLRAE LLFAVPAVLE ANPHLLGYYR LLMGYSQKEF YGRDKGFGVG CFKSMEEKGK 130 140 150 160 170 180 AGKAAVADLH DLCVAFCATA SALLAGVGPL RVSRELLDDL TLLTVGPQLR GGANNQRGAD 190 200 210 220 230 240 GIVLVFEIIR EIVAHAAAEV RESAIEVNSA TGRPVLIEFA PDPDIIIREE MENQHYRNVV 250 260 270 280 290 300 AIEVKSGTDV SNIHNRIGEA EKSHQKARQL GFTECWTVVN VGRLDMVKAR SESPSTDRFY 310 320 330 SLTALSLRAG DEYADFRRRV LSLTAIPARP ANP

References

[1]	"Structure and evolution of the XcyI restriction-modification system." Withers B., Ambroso L.A., Dunbar J.C. Nucleic Acids Res. 20:6267-6273(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 13D5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M98768 Genomic DNA. Translation: AAA27609.1.
PIR	S35550.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	2130. XcyI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019071. Restrct_endonuc_II_XcyI. [Graphical view]
Pfam	PF09571. RE_XcyI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2X1_XANCC

Accession

Primary (citable) accession number: P30773

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program