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P30773 (T2X1_XANCC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme XcyI

Short name=R.XcyI
EC=3.1.21.4
Alternative name(s):
Endonuclease XcyI
Type II restriction enzyme XcyI
Gene names
Name:xcyIR
OrganismXanthomonas campestris pv. cyanopsidis
Taxonomic identifier29444 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CCCGGG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Subunit structure

Monomer.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 333333Type-2 restriction enzyme XcyI
PRO_0000077371

Sequences

Sequence LengthMass (Da)Tools
P30773 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: DA0DCBB8CCDBD55A

FASTA33336,686
        10         20         30         40         50         60 
MTTPNKINFP PPKLQIDFAF ALKRFRAVYL QSALLETVRD MDIAELDRQL SKYVPPADLA 

        70         80         90        100        110        120 
TLAQYGLRAE LLFAVPAVLE ANPHLLGYYR LLMGYSQKEF YGRDKGFGVG CFKSMEEKGK 

       130        140        150        160        170        180 
AGKAAVADLH DLCVAFCATA SALLAGVGPL RVSRELLDDL TLLTVGPQLR GGANNQRGAD 

       190        200        210        220        230        240 
GIVLVFEIIR EIVAHAAAEV RESAIEVNSA TGRPVLIEFA PDPDIIIREE MENQHYRNVV 

       250        260        270        280        290        300 
AIEVKSGTDV SNIHNRIGEA EKSHQKARQL GFTECWTVVN VGRLDMVKAR SESPSTDRFY 

       310        320        330 
SLTALSLRAG DEYADFRRRV LSLTAIPARP ANP 

« Hide

References

[1]"Structure and evolution of the XcyI restriction-modification system."
Withers B., Ambroso L.A., Dunbar J.C.
Nucleic Acids Res. 20:6267-6273(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 13D5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M98768 Genomic DNA. Translation: AAA27609.1.
PIRS35550.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2130. XcyI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019071. Restrct_endonuc_II_XcyI.
[Graphical view]
PfamPF09571. RE_XcyI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2X1_XANCC
AccessionPrimary (citable) accession number: P30773
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries