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Protein

ATP-dependent helicase NAM7

Gene

NAM7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable helicase involved in mitochondrial functions. Required for rapid turnover of mRNAs containing a premature translational termination codon.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri61 – 208UPF1-typeAdd BLAST148
Nucleotide bindingi430 – 437ATPBy similarity8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • DNA binding Source: InterPro
  • ribosomal small subunit binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • chromatin silencing at silent mating-type cassette Source: SGD
  • DNA recombination Source: SGD
  • intracellular mRNA localization Source: SGD
  • nuclear-transcribed mRNA catabolic process Source: SGD
  • nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: SGD
  • protein ubiquitination Source: SGD
  • regulation of translational termination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Nonsense-mediated mRNA decay

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32782-MONOMER.
ReactomeiR-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase NAM7 (EC:3.6.4.-)
Alternative name(s):
Nonsense-mediated mRNA decay protein 1
Nuclear accommodation of mitochondria 7 protein
Up-frameshift suppressor 1
Gene namesi
Name:NAM7
Synonyms:IFS2, MOF4, UPF1
Ordered Locus Names:YMR080C
ORF Names:YM9582.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR080C.
SGDiS000004685. NAM7.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Present predominantly in the cytoplasm, but is also found in small quantities in the nucleus.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
  • polysome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000807131 – 971ATP-dependent helicase NAM7Add BLAST971

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei56PhosphoserineCombined sources1
Modified residuei869PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP30771.
PRIDEiP30771.

PTM databases

iPTMnetiP30771.

Interactioni

Protein-protein interaction databases

BioGridi35256. 666 interactors.
DIPiDIP-2373N.
IntActiP30771. 27 interactors.
MINTiMINT-492322.

Structurei

Secondary structure

1971
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni63 – 65Combined sources3
Turni70 – 72Combined sources3
Beta strandi73 – 76Combined sources4
Turni77 – 79Combined sources3
Beta strandi82 – 84Combined sources3
Beta strandi88 – 92Combined sources5
Helixi94 – 102Combined sources9
Beta strandi107 – 109Combined sources3
Beta strandi113 – 115Combined sources3
Beta strandi123 – 125Combined sources3
Turni130 – 132Combined sources3
Beta strandi133 – 136Combined sources4
Beta strandi145 – 148Combined sources4
Turni149 – 154Combined sources6
Helixi162 – 164Combined sources3
Beta strandi166 – 169Combined sources4
Beta strandi171 – 174Combined sources4
Turni176 – 178Combined sources3
Helixi186 – 189Combined sources4
Helixi195 – 205Combined sources11
Helixi235 – 257Combined sources23
Beta strandi267 – 271Combined sources5
Beta strandi277 – 281Combined sources5
Beta strandi298 – 303Combined sources6
Beta strandi305 – 309Combined sources5
Beta strandi311 – 319Combined sources9
Beta strandi328 – 332Combined sources5
Beta strandi345 – 351Combined sources7
Helixi356 – 370Combined sources15
Helixi377 – 384Combined sources8
Helixi411 – 420Combined sources10
Beta strandi424 – 429Combined sources6
Helixi436 – 451Combined sources16
Beta strandi455 – 461Combined sources7
Helixi462 – 474Combined sources13
Beta strandi479 – 481Combined sources3
Helixi485 – 487Combined sources3
Helixi495 – 497Combined sources3
Helixi499 – 504Combined sources6
Helixi510 – 521Combined sources12
Helixi526 – 545Combined sources20
Beta strandi548 – 553Combined sources6
Helixi556 – 558Combined sources3
Beta strandi567 – 571Combined sources5
Helixi574 – 576Combined sources3
Helixi579 – 586Combined sources8
Turni587 – 589Combined sources3
Beta strandi590 – 597Combined sources8
Helixi609 – 613Combined sources5
Turni614 – 617Combined sources4
Helixi620 – 626Combined sources7
Helixi642 – 652Combined sources11
Beta strandi658 – 661Combined sources4
Turni663 – 666Combined sources4
Beta strandi682 – 686Combined sources5
Beta strandi697 – 701Combined sources5
Helixi703 – 718Combined sources16
Helixi723 – 725Combined sources3
Beta strandi726 – 731Combined sources6
Helixi733 – 746Combined sources14
Helixi751 – 755Combined sources5
Beta strandi757 – 761Combined sources5
Helixi762 – 765Combined sources4
Beta strandi770 – 776Combined sources7
Helixi787 – 790Combined sources4
Helixi792 – 799Combined sources8
Beta strandi800 – 810Combined sources11
Helixi812 – 815Combined sources4
Helixi819 – 831Combined sources13
Beta strandi834 – 838Combined sources5
Beta strandi841 – 844Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XZLX-ray2.40A54-850[»]
ProteinModelPortaliP30771.
SMRiP30771.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30771.

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA2/NAM7 helicase family.Curated
Contains 1 UPF1-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri61 – 208UPF1-typeAdd BLAST148

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00800000124068.
HOGENOMiHOG000205990.
InParanoidiP30771.
KOiK14326.
OMAiPMMFWAN.
OrthoDBiEOG092C0D29.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR018999. RNA-helicase_UPF1_UPF2-interct.
[Graphical view]
PfamiPF09416. UPF1_Zn_bind. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

P30771-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGSGSHTPY DISNSPSDVN VQPATQLNST LVEDDDVDNQ LFEEAQVTET
60 70 80 90 100
GFRSPSASDN SCAYCGIDSA KCVIKCNSCK KWFCNTKNGT SSSHIVNHLV
110 120 130 140 150
LSHHNVVSLH PDSDLGDTVL ECYNCGRKNV FLLGFVSAKS EAVVVLLCRI
160 170 180 190 200
PCAQTKNANW DTDQWQPLIE DRQLLSWVAE QPTEEEKLKA RLITPSQISK
210 220 230 240 250
LEAKWRSNKD ATINDIDAPE EQEAIPPLLL RYQDAYEYQR SYGPLIKLEA
260 270 280 290 300
DYDKQLKESQ ALEHISVSWS LALNNRHLAS FTLSTFESNE LKVAIGDEMI
310 320 330 340 350
LWYSGMQHPD WEGRGYIVRL PNSFQDTFTL ELKPSKTPPP THLTTGFTAE
360 370 380 390 400
FIWKGTSYDR MQDALKKFAI DKKSISGYLY YKILGHQVVD ISFDVPLPKE
410 420 430 440 450
FSIPNFAQLN SSQSNAVSHV LQRPLSLIQG PPGTGKTVTS ATIVYHLSKI
460 470 480 490 500
HKDRILVCAP SNVAVDHLAA KLRDLGLKVV RLTAKSREDV ESSVSNLALH
510 520 530 540 550
NLVGRGAKGE LKNLLKLKDE VGELSASDTK RFVKLVRKTE AEILNKADVV
560 570 580 590 600
CCTCVGAGDK RLDTKFRTVL IDESTQASEP ECLIPIVKGA KQVILVGDHQ
610 620 630 640 650
QLGPVILERK AADAGLKQSL FERLISLGHV PIRLEVQYRM NPYLSEFPSN
660 670 680 690 700
MFYEGSLQNG VTIEQRTVPN SKFPWPIRGI PMMFWANYGR EEISANGTSF
710 720 730 740 750
LNRIEAMNCE RIITKLFRDG VKPEQIGVIT PYEGQRAYIL QYMQMNGSLD
760 770 780 790 800
KDLYIKVEVA SVDAFQGREK DYIILSCVRA NEQQAIGFLR DPRRLNVGLT
810 820 830 840 850
RAKYGLVILG NPRSLARNTL WNHLLIHFRE KGCLVEGTLD NLQLCTVQLV
860 870 880 890 900
RPQPRKTERP MNAQFNVESE MGDFPKFQDF DAQSMVSFSG QIGDFGNAFV
910 920 930 940 950
DNTELSSYIN NEYWNFENFK SAFSQKQNRN EIDDRNLYQE EASHLNSNFA
960 970
RELQREEQKH ELSKDFSNLG I
Length:971
Mass (Da):109,430
Last modified:July 1, 1993 - v1
Checksum:i9161AFB0BE6747FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62394 Genomic DNA. Translation: CAA44266.1.
M76659 Genomic DNA. Translation: AAA35197.1.
Z49259 Genomic DNA. Translation: CAA89226.1.
BK006946 Genomic DNA. Translation: DAA09978.1.
PIRiS23408.
RefSeqiNP_013797.1. NM_001182579.1.

Genome annotation databases

EnsemblFungiiYMR080C; YMR080C; YMR080C.
GeneIDi855104.
KEGGisce:YMR080C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62394 Genomic DNA. Translation: CAA44266.1.
M76659 Genomic DNA. Translation: AAA35197.1.
Z49259 Genomic DNA. Translation: CAA89226.1.
BK006946 Genomic DNA. Translation: DAA09978.1.
PIRiS23408.
RefSeqiNP_013797.1. NM_001182579.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XZLX-ray2.40A54-850[»]
ProteinModelPortaliP30771.
SMRiP30771.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35256. 666 interactors.
DIPiDIP-2373N.
IntActiP30771. 27 interactors.
MINTiMINT-492322.

PTM databases

iPTMnetiP30771.

Proteomic databases

MaxQBiP30771.
PRIDEiP30771.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR080C; YMR080C; YMR080C.
GeneIDi855104.
KEGGisce:YMR080C.

Organism-specific databases

EuPathDBiFungiDB:YMR080C.
SGDiS000004685. NAM7.

Phylogenomic databases

GeneTreeiENSGT00800000124068.
HOGENOMiHOG000205990.
InParanoidiP30771.
KOiK14326.
OMAiPMMFWAN.
OrthoDBiEOG092C0D29.

Enzyme and pathway databases

BioCyciYEAST:G3O-32782-MONOMER.
ReactomeiR-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiP30771.
PROiP30771.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR018999. RNA-helicase_UPF1_UPF2-interct.
[Graphical view]
PfamiPF09416. UPF1_Zn_bind. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiNAM7_YEAST
AccessioniPrimary (citable) accession number: P30771
Secondary accession number(s): D6VZQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6090 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.