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Protein

ATP-dependent helicase NAM7

Gene

NAM7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable helicase involved in mitochondrial functions. Required for rapid turnover of mRNAs containing a premature translational termination codon.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri61 – 208148UPF1-typeAdd
BLAST
Nucleotide bindingi430 – 4378ATPBy similarity

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • DNA binding Source: InterPro
  • ribosomal small subunit binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • chromatin silencing at silent mating-type cassette Source: SGD
  • DNA recombination Source: SGD
  • intracellular mRNA localization Source: SGD
  • nuclear-transcribed mRNA catabolic process Source: SGD
  • nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: SGD
  • protein ubiquitination Source: SGD
  • regulation of translational termination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Nonsense-mediated mRNA decay

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32782-MONOMER.
ReactomeiR-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase NAM7 (EC:3.6.4.-)
Alternative name(s):
Nonsense-mediated mRNA decay protein 1
Nuclear accommodation of mitochondria 7 protein
Up-frameshift suppressor 1
Gene namesi
Name:NAM7
Synonyms:IFS2, MOF4, UPF1
Ordered Locus Names:YMR080C
ORF Names:YM9582.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR080C.
SGDiS000004685. NAM7.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Present predominantly in the cytoplasm, but is also found in small quantities in the nucleus.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
  • polysome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 971971ATP-dependent helicase NAM7PRO_0000080713Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 561PhosphoserineCombined sources
Modified residuei869 – 8691PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP30771.
PeptideAtlasiP30771.

PTM databases

iPTMnetiP30771.

Interactioni

Protein-protein interaction databases

BioGridi35256. 665 interactions.
DIPiDIP-2373N.
IntActiP30771. 27 interactions.
MINTiMINT-492322.

Structurei

Secondary structure

1
971
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni63 – 653Combined sources
Turni70 – 723Combined sources
Beta strandi73 – 764Combined sources
Turni77 – 793Combined sources
Beta strandi82 – 843Combined sources
Beta strandi88 – 925Combined sources
Helixi94 – 1029Combined sources
Beta strandi107 – 1093Combined sources
Beta strandi113 – 1153Combined sources
Beta strandi123 – 1253Combined sources
Turni130 – 1323Combined sources
Beta strandi133 – 1364Combined sources
Beta strandi145 – 1484Combined sources
Turni149 – 1546Combined sources
Helixi162 – 1643Combined sources
Beta strandi166 – 1694Combined sources
Beta strandi171 – 1744Combined sources
Turni176 – 1783Combined sources
Helixi186 – 1894Combined sources
Helixi195 – 20511Combined sources
Helixi235 – 25723Combined sources
Beta strandi267 – 2715Combined sources
Beta strandi277 – 2815Combined sources
Beta strandi298 – 3036Combined sources
Beta strandi305 – 3095Combined sources
Beta strandi311 – 3199Combined sources
Beta strandi328 – 3325Combined sources
Beta strandi345 – 3517Combined sources
Helixi356 – 37015Combined sources
Helixi377 – 3848Combined sources
Helixi411 – 42010Combined sources
Beta strandi424 – 4296Combined sources
Helixi436 – 45116Combined sources
Beta strandi455 – 4617Combined sources
Helixi462 – 47413Combined sources
Beta strandi479 – 4813Combined sources
Helixi485 – 4873Combined sources
Helixi495 – 4973Combined sources
Helixi499 – 5046Combined sources
Helixi510 – 52112Combined sources
Helixi526 – 54520Combined sources
Beta strandi548 – 5536Combined sources
Helixi556 – 5583Combined sources
Beta strandi567 – 5715Combined sources
Helixi574 – 5763Combined sources
Helixi579 – 5868Combined sources
Turni587 – 5893Combined sources
Beta strandi590 – 5978Combined sources
Helixi609 – 6135Combined sources
Turni614 – 6174Combined sources
Helixi620 – 6267Combined sources
Helixi642 – 65211Combined sources
Beta strandi658 – 6614Combined sources
Turni663 – 6664Combined sources
Beta strandi682 – 6865Combined sources
Beta strandi697 – 7015Combined sources
Helixi703 – 71816Combined sources
Helixi723 – 7253Combined sources
Beta strandi726 – 7316Combined sources
Helixi733 – 74614Combined sources
Helixi751 – 7555Combined sources
Beta strandi757 – 7615Combined sources
Helixi762 – 7654Combined sources
Beta strandi770 – 7767Combined sources
Helixi787 – 7904Combined sources
Helixi792 – 7998Combined sources
Beta strandi800 – 81011Combined sources
Helixi812 – 8154Combined sources
Helixi819 – 83113Combined sources
Beta strandi834 – 8385Combined sources
Beta strandi841 – 8444Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XZLX-ray2.40A54-850[»]
ProteinModelPortaliP30771.
SMRiP30771. Positions 60-850.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30771.

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA2/NAM7 helicase family.Curated
Contains 1 UPF1-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri61 – 208148UPF1-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00800000124068.
HOGENOMiHOG000205990.
InParanoidiP30771.
KOiK14326.
OMAiHDSIGYI.
OrthoDBiEOG70PC5T.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR018999. RNA-helicase_UPF1_UPF2-interct.
[Graphical view]
PfamiPF09416. UPF1_Zn_bind. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

P30771-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGSGSHTPY DISNSPSDVN VQPATQLNST LVEDDDVDNQ LFEEAQVTET
60 70 80 90 100
GFRSPSASDN SCAYCGIDSA KCVIKCNSCK KWFCNTKNGT SSSHIVNHLV
110 120 130 140 150
LSHHNVVSLH PDSDLGDTVL ECYNCGRKNV FLLGFVSAKS EAVVVLLCRI
160 170 180 190 200
PCAQTKNANW DTDQWQPLIE DRQLLSWVAE QPTEEEKLKA RLITPSQISK
210 220 230 240 250
LEAKWRSNKD ATINDIDAPE EQEAIPPLLL RYQDAYEYQR SYGPLIKLEA
260 270 280 290 300
DYDKQLKESQ ALEHISVSWS LALNNRHLAS FTLSTFESNE LKVAIGDEMI
310 320 330 340 350
LWYSGMQHPD WEGRGYIVRL PNSFQDTFTL ELKPSKTPPP THLTTGFTAE
360 370 380 390 400
FIWKGTSYDR MQDALKKFAI DKKSISGYLY YKILGHQVVD ISFDVPLPKE
410 420 430 440 450
FSIPNFAQLN SSQSNAVSHV LQRPLSLIQG PPGTGKTVTS ATIVYHLSKI
460 470 480 490 500
HKDRILVCAP SNVAVDHLAA KLRDLGLKVV RLTAKSREDV ESSVSNLALH
510 520 530 540 550
NLVGRGAKGE LKNLLKLKDE VGELSASDTK RFVKLVRKTE AEILNKADVV
560 570 580 590 600
CCTCVGAGDK RLDTKFRTVL IDESTQASEP ECLIPIVKGA KQVILVGDHQ
610 620 630 640 650
QLGPVILERK AADAGLKQSL FERLISLGHV PIRLEVQYRM NPYLSEFPSN
660 670 680 690 700
MFYEGSLQNG VTIEQRTVPN SKFPWPIRGI PMMFWANYGR EEISANGTSF
710 720 730 740 750
LNRIEAMNCE RIITKLFRDG VKPEQIGVIT PYEGQRAYIL QYMQMNGSLD
760 770 780 790 800
KDLYIKVEVA SVDAFQGREK DYIILSCVRA NEQQAIGFLR DPRRLNVGLT
810 820 830 840 850
RAKYGLVILG NPRSLARNTL WNHLLIHFRE KGCLVEGTLD NLQLCTVQLV
860 870 880 890 900
RPQPRKTERP MNAQFNVESE MGDFPKFQDF DAQSMVSFSG QIGDFGNAFV
910 920 930 940 950
DNTELSSYIN NEYWNFENFK SAFSQKQNRN EIDDRNLYQE EASHLNSNFA
960 970
RELQREEQKH ELSKDFSNLG I
Length:971
Mass (Da):109,430
Last modified:July 1, 1993 - v1
Checksum:i9161AFB0BE6747FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62394 Genomic DNA. Translation: CAA44266.1.
M76659 Genomic DNA. Translation: AAA35197.1.
Z49259 Genomic DNA. Translation: CAA89226.1.
BK006946 Genomic DNA. Translation: DAA09978.1.
PIRiS23408.
RefSeqiNP_013797.1. NM_001182579.1.

Genome annotation databases

EnsemblFungiiYMR080C; YMR080C; YMR080C.
GeneIDi855104.
KEGGisce:YMR080C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62394 Genomic DNA. Translation: CAA44266.1.
M76659 Genomic DNA. Translation: AAA35197.1.
Z49259 Genomic DNA. Translation: CAA89226.1.
BK006946 Genomic DNA. Translation: DAA09978.1.
PIRiS23408.
RefSeqiNP_013797.1. NM_001182579.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XZLX-ray2.40A54-850[»]
ProteinModelPortaliP30771.
SMRiP30771. Positions 60-850.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35256. 665 interactions.
DIPiDIP-2373N.
IntActiP30771. 27 interactions.
MINTiMINT-492322.

PTM databases

iPTMnetiP30771.

Proteomic databases

MaxQBiP30771.
PeptideAtlasiP30771.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR080C; YMR080C; YMR080C.
GeneIDi855104.
KEGGisce:YMR080C.

Organism-specific databases

EuPathDBiFungiDB:YMR080C.
SGDiS000004685. NAM7.

Phylogenomic databases

GeneTreeiENSGT00800000124068.
HOGENOMiHOG000205990.
InParanoidiP30771.
KOiK14326.
OMAiHDSIGYI.
OrthoDBiEOG70PC5T.

Enzyme and pathway databases

BioCyciYEAST:G3O-32782-MONOMER.
ReactomeiR-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiP30771.
PROiP30771.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR018999. RNA-helicase_UPF1_UPF2-interct.
[Graphical view]
PfamiPF09416. UPF1_Zn_bind. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NAM7 nuclear gene encodes a novel member of a family of helicases with a Zn-ligand motif and is involved in mitochondrial functions in Saccharomyces cerevisiae."
    Altamura N., Groudinsky O., Dujardin G., Slonimski P.P.
    J. Mol. Biol. 224:575-587(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: R23/50.
  2. "Gene products that promote mRNA turnover in Saccharomyces cerevisiae."
    Leeds P.F., Wood J.M., Lee B.S., Culbertson M.R.
    Mol. Cell. Biol. 12:2165-2177(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Mof4-1 is an allele of the UPF1/IFS2 gene which affects both mRNA turnover and -1 ribosomal frameshifting efficiency."
    Cui Y., Dinman J.D., Peltz S.W.
    EMBO J. 15:5726-5736(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-869, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-869, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNAM7_YEAST
AccessioniPrimary (citable) accession number: P30771
Secondary accession number(s): D6VZQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 8, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6090 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.