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Protein

Elongation factor G

Gene

fusA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 278GTPBy similarity
Nucleotide bindingi84 – 885GTPBy similarity
Nucleotide bindingi138 – 1414GTPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor G
Short name:
EF-G
Gene namesi
Name:fusA
Synonyms:efg
Ordered Locus Names:ML1878
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1878.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 701701Elongation factor GPRO_0000091158Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272631.ML1878.

Structurei

3D structure databases

ProteinModelPortaliP30767.
SMRiP30767. Positions 7-695.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 287277tr-type GAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CEJ. Bacteria.
COG0480. LUCA.
HOGENOMiHOG000231585.
KOiK02355.
OMAiKLGVAIQ.
OrthoDBiEOG6X6RBF.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30767-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQKDVLTDL TKVRNIGIMA HIDAGKTTTT ERILYYTGIS YKIGEVHDGA
60 70 80 90 100
ATMDWMEQEQ ERGITITSAA TTCFWNDNQI NIIDTPGHVD FTVEVERSLR
110 120 130 140 150
VLDGAVAVFD GKEGVEPQSE QVWRQADKYE VPRICFVNKM DKIGADFYFS
160 170 180 190 200
VRTMQERLGA NVIPIQLPVG SEGDFEGVVD LVEMKAKVWS TEAKLGEKYD
210 220 230 240 250
VVGIPTDLQE KAEEYRTNLL ETVAETDEAL LEKYFSGEEL TVAEIKGAIR
260 270 280 290 300
KLTISSEAYP VLCGSAFKNK GVQPMLDAVI DYLPSPLDVP AAIGHVPGKE
310 320 330 340 350
DEEIVRKPST DEPLSALAFK VATHPFFGKL TYVRVYSGKV DSGSQVINAT
360 370 380 390 400
KGKKERLGKL FQMHSNKENP VETASAGHIY AVIGLKDTTT GDTLADPNNQ
410 420 430 440 450
IVLESMTFPD PVIEVAIEPK TKSDQEKLSL SIQKLAEEDP TFKVHLDSET
460 470 480 490 500
GQTVIGGMGE LHLDILVDRM RREFKVEANV GKPQVAYKET IRRVVETVEY
510 520 530 540 550
THKKQTGGSG QFAKVIIKLE PFSGENGATY EFENKVTGGR IPREYIPSVE
560 570 580 590 600
AGARDAMQYG VLAGYPLVNL KVTLLDGAYH DVDSSEIAFK IAGSQVLKKA
610 620 630 640 650
AAQAQPVILE PIMAVEVTTP EDYMGDVIGD LHSRRGQIQA MKERAGTRVV
660 670 680 690 700
RAHVPLSEMF GYVGDLRSKT QGRANYSMVF NSYSEVPANV SKEIIAKATG

E
Length:701
Mass (Da):77,161
Last modified:April 27, 2001 - v2
Checksum:iF18B724F35F3181E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti564 – 5641G → A in CAA78673 (PubMed:8446028).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14314 Genomic DNA. Translation: CAA78673.1.
AL583923 Genomic DNA. Translation: CAC30832.1.
PIRiH87143.
S31150.
RefSeqiNP_302268.1. NC_002677.1.
WP_010908589.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30832; CAC30832; CAC30832.
GeneIDi910612.
KEGGimle:ML1878.
PATRICi18057250. VBIMycLep78757_3552.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14314 Genomic DNA. Translation: CAA78673.1.
AL583923 Genomic DNA. Translation: CAC30832.1.
PIRiH87143.
S31150.
RefSeqiNP_302268.1. NC_002677.1.
WP_010908589.1. NC_002677.1.

3D structure databases

ProteinModelPortaliP30767.
SMRiP30767. Positions 7-695.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML1878.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC30832; CAC30832; CAC30832.
GeneIDi910612.
KEGGimle:ML1878.
PATRICi18057250. VBIMycLep78757_3552.

Organism-specific databases

LepromaiML1878.

Phylogenomic databases

eggNOGiENOG4105CEJ. Bacteria.
COG0480. LUCA.
HOGENOMiHOG000231585.
KOiK02355.
OMAiKLGVAIQ.
OrthoDBiEOG6X6RBF.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the first cosmid from the Mycobacterium leprae genome project: structure and function of the Rif-Str regions."
    Honore N.T., Bergh S., Chanteau S., Doucet-Populaire F., Eiglmeier K., Garnier T., Georges C., Launois P., Limpaiboon T., Newton S., Niang K., del Portillo P., Ramesh G.R., Reddi P., Ridel P.R., Sittisombut N., Wu-Hunter S., Cole S.T.
    Mol. Microbiol. 7:207-214(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiEFG_MYCLE
AccessioniPrimary (citable) accession number: P30767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: April 27, 2001
Last modified: July 6, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.