Reviewed, UniProtKB/Swiss-Prot P30759 (ARGI1_XENLA)

Names and origin

Protein names

Recommended name:
    Arginase-1
    EC=3.5.3.1
Alternative name(s):
    Type I arginase
    Arginase, hepatic

Gene names

Name:	arg1
Synonyms:	argl

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-arginine + H2O = L-ornithine + urea.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Subunit structure	Homotrimer.
Tissue specificity	Strongest expression in liver.
Developmental stage	First expressed at stage 10/11 of gastrulation. Higher levels found at stages 23/24 and 60-62. In the intestine, expression increased during metamorphosis (stage 62/64) and then, decreased. In the tail region, highest levels were found at the time of tail resorption. Activated, but at low levels, at stages 56-58 of hindlimb development. Ref.2
Induction	Slow-response activation by thyroid hormone (T3).
Sequence similarities	Belongs to the arginase family.

Ontologies

Keywords
Biological process	Arginine metabolism Urea cycle
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW urea cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arginase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Arginase-1		PRO_0000173698
Regions
	Region	126 – 130	5	Substrate binding By similarity
	Region	137 – 139	3	Substrate binding By similarity
Sites
	Metal binding	101	1	Manganese 1 By similarity
	Metal binding	124	1	Manganese 1 By similarity
	Metal binding	124	1	Manganese 2 By similarity
	Metal binding	126	1	Manganese 2 By similarity
	Metal binding	128	1	Manganese 1 By similarity
	Metal binding	232	1	Manganese 1 By similarity
	Metal binding	232	1	Manganese 2 By similarity
	Metal binding	234	1	Manganese 2 By similarity
	Binding site	183	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30759-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 8E6D08B20BF915ED	FASTA	316	34,566
	10 20 30 40 50 60 MAKERHSVGV IGAPFSKGQP RRGVEEGPKY LREAGLIEKL REFGNDVRDC GDLDFPDVPN 70 80 90 100 110 120 DTPFNNVKNP RTVGKATEIL ANAVTAVKKA DKTCQSIGGD HSLAVGTIAG HAAVHPNLCV 130 140 150 160 170 180 VWVDAHADIN TPSTSPCGNL HGQPLSFLMK ELKAKMPAVP GFEWVKPCLR SKDIVYIGLR 190 200 210 220 230 240 DVDPGEHYIL KTLGIKYLSM IEVDYLKDDK VMEETLEYLV GKHKRPIHLS FDIDGLDPSI 250 260 270 280 290 300 APATGTPCPG GRTYREGRIL HEQLHKTGLL SGVDTIWMES TSRGETKRDV EVTVKTALDM 310 TLSCFGKARE GFHAST

References

[1]	"Developmental and hormonal regulation of the Xenopus liver-type arginase gene." Xu Q., Baker B.S., Tata J.R. Eur. J. Biochem. 211:891-898(1993) [PubMed: 7916684] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Thyroid hormone-dependent differential regulation of multiple arginase genes during amphibian metamorphosis." Patterton D., Shi Y.-B. J. Biol. Chem. 269:25328-25334(1994) [PubMed: 7929226] [Abstract] Cited for: DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X69820 mRNA. Translation: CAA49474.1.
PIR	S29394.
RefSeq	NP_001095269.1.
UniGene	Xl.57156
3D structure databases
HSSP	HSSP built from PDB template 1RLA based on UniProtKB P07824.
ModBase	Search...
Genome annotation databases
GeneID	779061.
KEGG	xla:779061.
Phylogenomic databases
HOVERGEN	P30759.
Enzyme and pathway databases
BRENDA	3.5.3.1. 648.
Family and domain databases
InterPro	IPR005924. Arginase. IPR014033. Arginase_sub. IPR006035. Ureohydrolase. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
PANTHER	PTHR11358:SF2. Arginase_sub. 1 hit. PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PRINTS	PR00116. ARGINASE.
TIGRFAMs	TIGR01229. rocF_arginase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGI1_XENLA

Accession

Primary (citable) accession number: P30759

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project