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Protein

Methionine import ATP-binding protein MetN

Gene

metN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system (Probable). It has also been shown to be involved in formyl-L-methionine transport.UniRule annotationCurated2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 458ATPUniRule annotation

GO - Molecular functioni

  • amino acid-transporting ATPase activity Source: UniProtKB-HAMAP
  • ATPase activity Source: EcoliWiki
  • ATP binding Source: EcoliWiki
  • D-methionine transmembrane transporter activity Source: EcoliWiki

GO - Biological processi

  • D-methionine transport Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ABC-MONOMER.
ECOL316407:JW0195-MONOMER.
MetaCyc:ABC-MONOMER.

Protein family/group databases

TCDBi3.A.1.24.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine import ATP-binding protein MetNUniRule annotation (EC:3.6.3.-UniRule annotation)
Gene namesi
Name:metNUniRule annotation
Synonyms:abc
Ordered Locus Names:b0199, JW0195
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11621. metN.

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Methionine import ATP-binding protein MetNPRO_0000092503Add
BLAST

Proteomic databases

PaxDbiP30750.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (MetN), two transmembrane proteins (MetI) and a solute-binding protein (MetQ).UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
hycGP164335EBI-541886,EBI-541977
metIP315472EBI-541886,EBI-8769561
mntRP0A9F13EBI-541886,EBI-541895

Protein-protein interaction databases

BioGridi4259755. 20 interactions.
DIPiDIP-9027N.
IntActiP30750. 55 interactions.
MINTiMINT-1223626.
STRINGi511145.b0199.

Structurei

Secondary structure

1
343
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 1211Combined sources
Beta strandi14 – 2815Combined sources
Beta strandi33 – 375Combined sources
Helixi44 – 518Combined sources
Beta strandi58 – 647Combined sources
Helixi74 – 818Combined sources
Beta strandi84 – 874Combined sources
Helixi99 – 10911Combined sources
Helixi114 – 12714Combined sources
Helixi131 – 1333Combined sources
Turni138 – 1403Combined sources
Helixi143 – 15412Combined sources
Turni155 – 1573Combined sources
Beta strandi160 – 1667Combined sources
Turni167 – 1704Combined sources
Helixi173 – 18917Combined sources
Beta strandi193 – 1997Combined sources
Helixi201 – 2077Combined sources
Beta strandi209 – 2157Combined sources
Beta strandi218 – 2214Combined sources
Helixi225 – 2295Combined sources
Helixi235 – 24410Combined sources
Helixi251 – 2566Combined sources
Beta strandi258 – 2603Combined sources
Beta strandi265 – 27612Combined sources
Beta strandi278 – 2803Combined sources
Helixi282 – 2898Combined sources
Beta strandi293 – 30412Combined sources
Beta strandi307 – 31812Combined sources
Helixi320 – 33213Combined sources
Beta strandi336 – 3438Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DHWX-ray3.70C/D/G/H1-343[»]
3DHXX-ray2.10A/B246-343[»]
3TUIX-ray2.90C/D/G/H1-343[»]
3TUJX-ray4.00C/D1-343[»]
3TUZX-ray3.40C/D/G/H1-343[»]
ProteinModelPortaliP30750.
SMRiP30750. Positions 1-343.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30750.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 241240ABC transporterUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Methionine importer (TC 3.A.1.24) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JEI. Bacteria.
COG1135. LUCA.
InParanoidiP30750.
KOiK02071.
OMAiHDAYPAN.
OrthoDBiEOG6T7N3V.
PhylomeDBiP30750.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR026253. ABC_MetN.
IPR012692. ABC_MetN_proteobac.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017908. ABC_transprt_methionine_MetN_C.
IPR018449. NIL_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24220:SF455. PTHR24220:SF455. 1 hit.
PfamiPF00005. ABC_tran. 1 hit.
PF09383. NIL. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00930. NIL. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02314. ABC_MetN. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51264. METN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30750-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKLSNITKV FHQGTRTIQA LNNVSLHVPA GQIYGVIGAS GAGKSTLIRC
60 70 80 90 100
VNLLERPTEG SVLVDGQELT TLSESELTKA RRQIGMIFQH FNLLSSRTVF
110 120 130 140 150
GNVALPLELD NTPKDEVKRR VTELLSLVGL GDKHDSYPSN LSGGQKQRVA
160 170 180 190 200
IARALASNPK VLLCDEATSA LDPATTRSIL ELLKDINRRL GLTILLITHE
210 220 230 240 250
MDVVKRICDC VAVISNGELI EQDTVSEVFS HPKTPLAQKF IQSTLHLDIP
260 270 280 290 300
EDYQERLQAE PFTDCVPMLR LEFTGQSVDA PLLSETARRF NVNNNIISAQ
310 320 330 340
MDYAGGVKFG IMLTEMHGTQ QDTQAAIAWL QEHHVKVEVL GYV
Length:343
Mass (Da):37,788
Last modified:November 1, 1997 - v4
Checksum:i2FC796C605216FB3
GO

Sequence cautioni

The sequence AAC36869.1 differs from that shown. Reason: Frameshift at position 200. Curated
The sequence BAA03659.1 differs from that shown. Reason: Frameshift at positions 106 and 156. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti165 – 1651D → V in AAC36869 (PubMed:7904973).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15061 Genomic DNA. Translation: BAA03659.1. Frameshift.
U70214 Genomic DNA. Translation: AAB08627.1.
U00096 Genomic DNA. Translation: AAC73310.1.
AP009048 Genomic DNA. Translation: BAA77876.2.
L08626 Unassigned DNA. Translation: AAC36869.1. Frameshift.
PIRiG64744.
RefSeqiNP_414741.1. NC_000913.3.
WP_000594006.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73310; AAC73310; b0199.
BAA77876; BAA77876; BAA77876.
GeneIDi944896.
KEGGiecj:JW0195.
eco:b0199.
PATRICi32115511. VBIEscCol129921_0207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15061 Genomic DNA. Translation: BAA03659.1. Frameshift.
U70214 Genomic DNA. Translation: AAB08627.1.
U00096 Genomic DNA. Translation: AAC73310.1.
AP009048 Genomic DNA. Translation: BAA77876.2.
L08626 Unassigned DNA. Translation: AAC36869.1. Frameshift.
PIRiG64744.
RefSeqiNP_414741.1. NC_000913.3.
WP_000594006.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DHWX-ray3.70C/D/G/H1-343[»]
3DHXX-ray2.10A/B246-343[»]
3TUIX-ray2.90C/D/G/H1-343[»]
3TUJX-ray4.00C/D1-343[»]
3TUZX-ray3.40C/D/G/H1-343[»]
ProteinModelPortaliP30750.
SMRiP30750. Positions 1-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259755. 20 interactions.
DIPiDIP-9027N.
IntActiP30750. 55 interactions.
MINTiMINT-1223626.
STRINGi511145.b0199.

Protein family/group databases

TCDBi3.A.1.24.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP30750.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73310; AAC73310; b0199.
BAA77876; BAA77876; BAA77876.
GeneIDi944896.
KEGGiecj:JW0195.
eco:b0199.
PATRICi32115511. VBIEscCol129921_0207.

Organism-specific databases

EchoBASEiEB1578.
EcoGeneiEG11621. metN.

Phylogenomic databases

eggNOGiENOG4108JEI. Bacteria.
COG1135. LUCA.
InParanoidiP30750.
KOiK02071.
OMAiHDAYPAN.
OrthoDBiEOG6T7N3V.
PhylomeDBiP30750.

Enzyme and pathway databases

BioCyciEcoCyc:ABC-MONOMER.
ECOL316407:JW0195-MONOMER.
MetaCyc:ABC-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP30750.
PROiP30750.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR026253. ABC_MetN.
IPR012692. ABC_MetN_proteobac.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017908. ABC_transprt_methionine_MetN_C.
IPR018449. NIL_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24220:SF455. PTHR24220:SF455. 1 hit.
PfamiPF00005. ABC_tran. 1 hit.
PF09383. NIL. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00930. NIL. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02314. ABC_MetN. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51264. METN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of 5'flanking region of the ribosomal RNA gene (rrnH) in E. coli."
    Miyamoto K., Inokuchi H.
    Submitted (APR-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
    Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 106.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Cloning and organization of the abc and mdl genes of Escherichia coli: relationship to eukaryotic multidrug resistance."
    Allikmets R., Gerrard B.C., Court D., Dean M.C.
    Gene 136:231-236(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-231.
    Strain: TAP90 / ATCC 47037.
  7. "The metD D-methionine transporter locus of Escherichia coli is an ABC transporter gene cluster."
    Gal J., Szvetnik A., Schnell R., Kalman M.
    J. Bacteriol. 184:4930-4932(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN METHIONINE TRANSPORT.
    Strain: K12 / MG1655 / ATCC 47076.
  8. "A transporter of Escherichia coli specific for L- and D-methionine is the prototype for a new family within the ABC superfamily."
    Zhang Z., Feige J.N., Chang A.B., Anderson I.J., Brodianski V.M., Vitreschak A.G., Gelfand M.S., Saier M.H. Jr.
    Arch. Microbiol. 180:88-100(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN METHIONINE AND FORMYL-L-METHIONINE TRANSPORT.
    Strain: K12.

Entry informationi

Entry nameiMETN_ECOLI
AccessioniPrimary (citable) accession number: P30750
Secondary accession number(s): P77517, Q47615
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: January 20, 2016
This is version 143 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.