P30750 (METN_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine import ATP-binding protein MetN
EC=3.6.3.-

Gene names

Name:	metN
Synonyms:	abc
Ordered Locus Names:	b0199, JW0195

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system Probable. It has also been shown to be involved in formyl-L-methionine transport. Ref.7 Ref.8
Subunit structure	The complex is composed of two ATP-binding proteins (MetN), two transmembrane proteins (MetI) and a solute-binding protein (MetQ) Potential.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. Methionine importer (TC 3.A.1.24) family. [View classification] Contains 1 ABC transporter domain.
Sequence caution	The sequence AAC36869.1 differs from that shown. Reason: Frameshift at position 200. The sequence BAA03659.1 differs from that shown. Reason: Frameshift at positions 106 and 156.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from direct assay PubMed 18621668. Source: EcoliWiki ATPase activity Inferred from direct assay PubMed 18621668. Source: EcoliWiki D-methionine transmembrane transporter activity Inferred from direct assay PubMed 18621668. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 343

343

Methionine import ATP-binding protein MetN

PRO_0000092503

Regions

Domain

2 – 241

240

ABC transporter

Nucleotide binding

38 – 45

8

ATP Potential

Experimental info

Sequence conflict

165

1

D → V in AAC36869. Ref.6

Secondary structure

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343

Helix Strand Turn

Details...

Beta strand

2 – 12

11

Beta strand

14 – 28

15

Beta strand

33 – 37

5

Helix

44 – 51

8

Beta strand

58 – 64

7

Helix

74 – 81

8

Beta strand

84 – 87

4

Helix

99 – 109

11

Helix

114 – 127

14

Helix

131 – 133

3

Turn

138 – 140

3

Helix

143 – 154

12

Turn

155 – 157

3

Beta strand

160 – 166

7

Turn

167 – 170

4

Helix

173 – 189

17

Beta strand

193 – 199

7

Helix

201 – 207

7

Beta strand

209 – 215

7

Beta strand

218 – 221

4

Helix

225 – 229

5

Helix

235 – 244

10

Helix

251 – 256

6

Beta strand

258 – 260

3

Beta strand

265 – 276

12

Beta strand

278 – 280

3

Helix

282 – 289

8

Beta strand

293 – 304

12

Beta strand

307 – 318

12

Helix

320 – 332

13

Beta strand

336 – 343

8

Sequences

	Sequence		Length	Mass (Da)
	P30750 [UniParc]. Last modified November 1, 1997. Version 4. Checksum: 2FC796C605216FB3	FASTA	343	37,788
	10 20 30 40 50 60 MIKLSNITKV FHQGTRTIQA LNNVSLHVPA GQIYGVIGAS GAGKSTLIRC VNLLERPTEG 70 80 90 100 110 120 SVLVDGQELT TLSESELTKA RRQIGMIFQH FNLLSSRTVF GNVALPLELD NTPKDEVKRR 130 140 150 160 170 180 VTELLSLVGL GDKHDSYPSN LSGGQKQRVA IARALASNPK VLLCDEATSA LDPATTRSIL 190 200 210 220 230 240 ELLKDINRRL GLTILLITHE MDVVKRICDC VAVISNGELI EQDTVSEVFS HPKTPLAQKF 250 260 270 280 290 300 IQSTLHLDIP EDYQERLQAE PFTDCVPMLR LEFTGQSVDA PLLSETARRF NVNNNIISAQ 310 320 330 340 MDYAGGVKFG IMLTEMHGTQ QDTQAAIAWL QEHHVKVEVL GYV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of 5'flanking region of the ribosomal RNA gene (rrnH) in E. coli." Miyamoto K., Inokuchi H. Submitted (APR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 106. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Cloning and organization of the abc and mdl genes of Escherichia coli: relationship to eukaryotic multidrug resistance." Allikmets R., Gerrard B.C., Court D., Dean M.C. Gene 136:231-236(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-231. Strain: TAP90 / ATCC 47037.
[7]	"The metD D-methionine transporter locus of Escherichia coli is an ABC transporter gene cluster." Gal J., Szvetnik A., Schnell R., Kalman M. J. Bacteriol. 184:4930-4932(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN METHIONINE TRANSPORT. Strain: K12 / MG1655 / ATCC 47076.
[8]	"A transporter of Escherichia coli specific for L- and D-methionine is the prototype for a new family within the ABC superfamily." Zhang Z., Feige J.N., Chang A.B., Anderson I.J., Brodianski V.M., Vitreschak A.G., Gelfand M.S., Saier M.H. Jr. Arch. Microbiol. 180:88-100(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN METHIONINE AND FORMYL-L-METHIONINE TRANSPORT. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D15061 Genomic DNA. Translation: BAA03659.1. Frameshift.
U70214 Genomic DNA. Translation: AAB08627.1.
U00096 Genomic DNA. Translation: AAC73310.1.
AP009048 Genomic DNA. Translation: BAA77876.2.
L08626 Unassigned DNA. Translation: AAC36869.1. Frameshift.

PIR

G64744.

RefSeq

NP_414741.1. NC_000913.2.
YP_488502.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DHW	X-ray	3.70	C/D/G/H	1-343	[»]
3DHX	X-ray	2.10	A/B	246-343	[»]
3TUI	X-ray	2.90	C/D/G/H	1-343	[»]
3TUJ	X-ray	4.00	C/D	1-343	[»]
3TUZ	X-ray	3.40	C/D/G/H	1-343	[»]

ProteinModelPortal

P30750.

SMR

P30750. Positions 1-343.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-9027N.

IntAct

P30750. 16 interactions.

MINT

MINT-1223626.

STRING

511145.b0199.

Protein family/group databases

TCDB

3.A.1.24.1. ATP-binding cassette (ABC) superfamily.

Proteomic databases

PaxDb

P30750.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73310; AAC73310; b0199.
BAA77876; BAA77876; BAA77876.

GeneID

12930761.
944896.

KEGG

ecj:Y75_p0196.
eco:b0199.

PATRIC

32115511. VBIEscCol129921_0207.

Organism-specific databases

EchoBASE

EB1578.

EcoGene

EG11621. metN.

Phylogenomic databases

eggNOG

COG1135.

KO

K02071.

OMA

VNMLEAP.

ProtClustDB

PRK11153.

Enzyme and pathway databases

BioCyc

EcoCyc:ABC-MONOMER.
ECOL316407:JW0195-MONOMER.

Gene expression databases

Genevestigator

P30750.

Family and domain databases

InterPro

IPR003593. AAA+_ATPase.
IPR026253. ABC_MetN.
IPR012692. ABC_MetN_proteobac.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017908. ABC_transprt_methionine_MetN_C.
IPR018449. NIL_domain.
[Graphical view]

PANTHER

PTHR24220:SF186. PTHR24220:SF186. 1 hit.

Pfam

PF00005. ABC_tran. 1 hit.
PF09383. NIL. 1 hit.
[Graphical view]

SMART

SM00382. AAA. 1 hit.
SM00930. NIL. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02314. ABC_MetN. 1 hit.

PROSITE

PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51264. METN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P30750.

Entry information

Entry name

METN_ECOLI

Accession

Primary (citable) accession number: P30750
Secondary accession number(s): P77517, Q47615

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 120 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program