Methionine import ATP-binding protein MetN - Escherichia coli (strain K12)

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P30750 (METN_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Methionine import ATP-binding protein MetN
EC=3.6.3.-

Gene names

Name:	metN
Synonyms:	abc
Ordered Locus Names:	b0199, JW0195

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system Probable. It has also been shown to be involved in formyl-L-methionine transport. Ref.7 Ref.8
Subunit structure	The complex is composed of two ATP-binding proteins (MetN), two transmembrane proteins (MetI) and a solute-binding protein (MetQ) Potential.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity HAMAP MF_01719.
Sequence similarities	Belongs to the ABC transporter superfamily. Methionine importer (TC 3.A.1.24) family. [View classification] Contains 1 ABC transporter domain.
Sequence caution	The sequence AAC36869.1 differs from that shown. Reason: Frameshift at position 200. The sequence BAA03659.1 differs from that shown. Reason: Frameshift at positions 106 and 156.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from direct assay. Source: EcoliWiki D-methionine transmembrane transporter activity Inferred from direct assay. Source: EcoliWiki amino acid-transporting ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 343

343

Methionine import ATP-binding protein MetN HAMAP MF_01719

PRO_0000092503

Regions

Domain

2 – 241

240

ABC transporter

Nucleotide binding

38 – 45

ATP Potential

Experimental info

Sequence conflict

165

D → V in AAC36869. Ref.6

Secondary structure

343

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P30750 [UniParc].

Last modified November 1, 1997. Version 4.
Checksum: 2FC796C605216FB3
Show »

FASTA

343

37,788

        10         20         30         40         50         60 
MIKLSNITKV FHQGTRTIQA LNNVSLHVPA GQIYGVIGAS GAGKSTLIRC VNLLERPTEG 

        70         80         90        100        110        120 
SVLVDGQELT TLSESELTKA RRQIGMIFQH FNLLSSRTVF GNVALPLELD NTPKDEVKRR 

       130        140        150        160        170        180 
VTELLSLVGL GDKHDSYPSN LSGGQKQRVA IARALASNPK VLLCDEATSA LDPATTRSIL 

       190        200        210        220        230        240 
ELLKDINRRL GLTILLITHE MDVVKRICDC VAVISNGELI EQDTVSEVFS HPKTPLAQKF 

       250        260        270        280        290        300 
IQSTLHLDIP EDYQERLQAE PFTDCVPMLR LEFTGQSVDA PLLSETARRF NVNNNIISAQ 

       310        320        330        340 
MDYAGGVKFG IMLTEMHGTQ QDTQAAIAWL QEHHVKVEVL GYV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of 5'flanking region of the ribosomal RNA gene (rrnH) in E. coli." Miyamoto K., Inokuchi H. Submitted (APR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 106. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Cloning and organization of the abc and mdl genes of Escherichia coli: relationship to eukaryotic multidrug resistance." Allikmets R., Gerrard B.C., Court D., Dean M.C. Gene 136:231-236(1993) [PubMed: 7904973] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-231. Strain: TAP90 / ATCC 47037.
[7]	"The metD D-methionine transporter locus of Escherichia coli is an ABC transporter gene cluster." Gal J., Szvetnik A., Schnell R., Kalman M. J. Bacteriol. 184:4930-4932(2002) [PubMed: 12169620] [Abstract] Cited for: FUNCTION IN METHIONINE TRANSPORT. Strain: K12 / MG1655 / ATCC 47076.
[8]	"A transporter of Escherichia coli specific for L- and D-methionine is the prototype for a new family within the ABC superfamily." Zhang Z., Feige J.N., Chang A.B., Anderson I.J., Brodianski V.M., Vitreschak A.G., Gelfand M.S., Saier M.H. Jr. Arch. Microbiol. 180:88-100(2003) [PubMed: 12819857] [Abstract] Cited for: FUNCTION IN METHIONINE AND FORMYL-L-METHIONINE TRANSPORT. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D15061 Genomic DNA. Translation: BAA03659.1. Frameshift.
U70214 Genomic DNA. Translation: AAB08627.1.
U00096 Genomic DNA. Translation: AAC73310.1.
AP009048 Genomic DNA. Translation: BAA77876.2.
L08626 Unassigned DNA. Translation: AAC36869.1. Frameshift.

PIR

G64744.

RefSeq

NP_414741.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DHW	X-ray	3.70	C/D/G/H	1-343	[»]
3DHX	X-ray	2.10	A/B	246-343	[»]
3TUI	X-ray	2.90	C/D/G/H	1-343	[»]
3TUJ	X-ray	4.00	C/D	1-343	[»]
3TUZ	X-ray	3.40	C/D/G/H	1-343	[»]

ProteinModelPortal

P30750.

SMR

P30750. Positions 1-343.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-9027N.

IntAct

P30750. 16 interactions.

MINT

MINT-1223626.

Protein family/group databases

TCDB

3.A.1.24.1. ATP-binding cassette (ABC) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004161; EBESCP00000004161; EBESCG00000003397.
EBESCT00000018129; EBESCP00000017420; EBESCG00000017184.

GeneID

944896.

GenomeReviews

Gene locus JW0195 in contig AP009048_GR.
Gene locus b0199 in contig U00096_GR.

KEGG

ecj:JW0195.
eco:b0199.

PATRIC

32115511. VBIEscCol129921_0207.

Organism-specific databases

EchoBASE

EB1578.

EcoGene

EG11621. metN.

Phylogenomic databases

eggNOG

COG1135.

GeneTree

EBGT00050000008233.

HOGENOM

HBG758042.

OMA

VNMLEAP.

PhylomeDB

P30750.

ProtClustDB

PRK11153.

Enzyme and pathway databases

BioCyc

EcoCyc:ABC-MONOMER.

Gene expression databases

Genevestigator

P30750.

Family and domain databases

HAMAP

MF_01719. ABC_ATPase_MetN.
[Tree]

InterPro

IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR012692. ABC_transprt_methionine_MetN.
IPR017908. ABC_transprt_methionine_MetN_C.
IPR003593. ATPase_AAA+_core.
IPR018449. NIL_domain.
[Graphical view]

K02071.

Pfam

PF00005. ABC_tran. 1 hit.
PF09383. NIL. 1 hit.
[Graphical view]

SMART

SM00382. AAA. 1 hit.
SM00930. NIL. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02314. ABC_MetN. 1 hit.

PROSITE

PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51264. METN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

METN_ECOLI

Accession

Primary (citable) accession number: P30750
Secondary accession number(s): P77517, Q47615

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 109 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents