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Protein

GTP 3',8-cyclase

Gene

moaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z).
Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.UniRule annotation

Catalytic activityi

GTP + S-adenosyl-L-methionine + reduced electron acceptor = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotation

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17GTPUniRule annotation1
Metal bindingi24Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi28Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei30S-adenosyl-L-methionineUniRule annotation1
Metal bindingi31Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei68GTPUniRule annotation1
Binding sitei72S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei99GTPUniRule annotation1
Binding sitei123S-adenosyl-L-methionineUniRule annotation1
Binding sitei160GTPUniRule annotation1
Binding sitei194S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi257Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi260Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi274Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi262 – 264GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

  • molybdopterin cofactor biosynthetic process Source: EcoCyc
  • Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  • response to heat Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11595-MONOMER.
ECOL316407:JW0764-MONOMER.
MetaCyc:EG11595-MONOMER.
UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP 3',8-cyclaseUniRule annotation (EC:4.1.99.22UniRule annotation)
Alternative name(s):
Molybdenum cofactor biosynthesis protein AUniRule annotation
Gene namesi
Name:moaAUniRule annotation
Synonyms:bisA, chlA, chlA1, narA
Ordered Locus Names:b0781, JW0764
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11595. moaA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001529581 – 329GTP 3',8-cyclaseAdd BLAST329

Proteomic databases

PaxDbiP30745.
PRIDEiP30745.

Expressioni

Inductioni

By anaerobiosis, repressed by the molybdenum cofactor.

Interactioni

Subunit structurei

Monomer and homodimer.UniRule annotation

Protein-protein interaction databases

BioGridi4259951. 5 interactors.
DIPiDIP-10228N.
IntActiP30745. 13 interactors.
MINTiMINT-1229909.
STRINGi511145.b0781.

Structurei

3D structure databases

ProteinModelPortaliP30745.
SMRiP30745.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CM1. Bacteria.
COG2896. LUCA.
HOGENOMiHOG000228682.
InParanoidiP30745.
KOiK03639.
OMAiGWIHQIR.
PhylomeDBiP30745.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQLTDAFA RKFYYLRLSI TDVCNFRCTY CLPDGYKPSG VTNKGFLTVD
60 70 80 90 100
EIRRVTRAFA RLGTEKVRLT GGEPSLRRDF TDIIAAVREN DAIRQIAVTT
110 120 130 140 150
NGYRLERDVA SWRDAGLTGI NVSVDSLDAR QFHAITGQDK FNQVMAGIDA
160 170 180 190 200
AFEAGFEKVK VNTVLMRDVN HHQLDTFLNW IQHRPIQLRF IELMETGEGS
210 220 230 240 250
ELFRKHHISG QVLRDELLRR GWIHQLRQRS DGPAQVFCHP DYAGEIGLIM
260 270 280 290 300
PYEKDFCATC NRLRVSSIGK LHLCLFGEGG VNLRDLLEDD TQQQALEARI
310 320
SAALREKKQT HFLHQNNTGI TQNLSYIGG
Length:329
Mass (Da):37,346
Last modified:April 1, 1993 - v1
Checksum:iFE91263C53F33F78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70420 Genomic DNA. Translation: CAA49861.1.
U00096 Genomic DNA. Translation: AAC73868.1.
AP009048 Genomic DNA. Translation: BAA35439.1.
PIRiS31879.
RefSeqiNP_415302.1. NC_000913.3.
WP_001350494.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73868; AAC73868; b0781.
BAA35439; BAA35439; BAA35439.
GeneIDi945392.
KEGGiecj:JW0764.
eco:b0781.
PATRICi32116763. VBIEscCol129921_0807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70420 Genomic DNA. Translation: CAA49861.1.
U00096 Genomic DNA. Translation: AAC73868.1.
AP009048 Genomic DNA. Translation: BAA35439.1.
PIRiS31879.
RefSeqiNP_415302.1. NC_000913.3.
WP_001350494.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP30745.
SMRiP30745.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259951. 5 interactors.
DIPiDIP-10228N.
IntActiP30745. 13 interactors.
MINTiMINT-1229909.
STRINGi511145.b0781.

Proteomic databases

PaxDbiP30745.
PRIDEiP30745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73868; AAC73868; b0781.
BAA35439; BAA35439; BAA35439.
GeneIDi945392.
KEGGiecj:JW0764.
eco:b0781.
PATRICi32116763. VBIEscCol129921_0807.

Organism-specific databases

EchoBASEiEB1552.
EcoGeneiEG11595. moaA.

Phylogenomic databases

eggNOGiENOG4105CM1. Bacteria.
COG2896. LUCA.
HOGENOMiHOG000228682.
InParanoidiP30745.
KOiK03639.
OMAiGWIHQIR.
PhylomeDBiP30745.

Enzyme and pathway databases

UniPathwayiUPA00344.
BioCyciEcoCyc:EG11595-MONOMER.
ECOL316407:JW0764-MONOMER.
MetaCyc:EG11595-MONOMER.

Miscellaneous databases

PROiP30745.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOAA_ECOLI
AccessioniPrimary (citable) accession number: P30745
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 30, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.