P30744 (SDHM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-serine dehydratase 2
Short name=SDH 2
EC=4.3.1.17

Alternative name(s):
L-serine deaminase 2
Short name=L-SD2

Gene names

Name:	sdaB
Ordered Locus Names:	b2797, JW2768

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Deaminates also threonine, particularly when it is present in high concentration.
Catalytic activity	L-serine = pyruvate + NH3.
Cofactor	Binds 1 4Fe-4S cluster Probable.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Induction	Transcribed in rich medium, particularly in the absence of glucose, and is under the control of catabolite activator protein. It is made aerobically and anaerobically.
Post-translational modification	Activated by post-translational modification by a system involving at least three gene products. Activation is mimicked in vitro by iron and dithiothreitol. There is considerable evidence for a free-radical activation mechanism.
Sequence similarities	Belongs to the iron-sulfur dependent L-serine dehydratase family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-serine catabolic process Inferred from direct assay. Source: EcoCyc gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW L-serine ammonia-lyase activity Inferred from direct assay. Source: EcoCyc metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
blc	P0A901	1	EBI-371146,EBI-1114945

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	L-serine dehydratase 2		PRO_0000171904
Experimental info
	Sequence conflict	132	1	L → V Ref.1
	Sequence conflict	134	1	G → A Ref.1
	Sequence conflict	196 – 197	2	EL → DV Ref.1
	Sequence conflict	236 – 239	4	VPRR → STPC Ref.1
	Sequence conflict	360 – 362	3	ASP → GNR Ref.1
	Sequence conflict	372	1	A → G Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P30744 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 47BCD8E880202008	FASTA	455	48,753
	10 20 30 40 50 60 MISVFDIFKI GIGPSSSHTV GPMKAGKQFT DDLIARNLLK DVTRVVVDVY GSLSLTGKGH 70 80 90 100 110 120 HTDIAIIMGL AGNLPDTVDI DSIPSFIQDV NTHGRLMLAN GQHEVEFPVD QCMNFHADNL 130 140 150 160 170 180 SLHENGMRIT ALAGDKVVYS QTYYSIGGGF IVDEEHFGQQ DSAPVEVPYP YSSAADLQKH 190 200 210 220 230 240 CQETGLSLSG LMMKNELALH SKEELEQHLA NVWEVMRGGI ERGISTEGVL PGKLRVPRRA 250 260 270 280 290 300 AALRRMLVSQ DKTTTDPMAV VDWINMFALA VNEENAAGGR VVTAPTNGAC GIIPAVLAYY 310 320 330 340 350 360 DKFIREVNAN SLARYLLVAS AIGSLYKMNA SISGAEVGCQ GEVGVACSMA AAGLAELLGA 370 380 390 400 410 420 SPAQVCIAAE IAMEHNLGLT CDPVAGQVQV PCIERNAIAA VKAVNAARMA LRRTSEPRVC 430 440 450 LDKVIETMYE TGKDMNAKYR ETSRGGLAMK IVACD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and characterization of the sdaB gene from Escherichia coli K-12." Shao Z., Newman E.B. Eur. J. Biochem. 212:777-784(1993) [PubMed: 8385012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07763 Genomic DNA. No translation available. U29581 Genomic DNA. Translation: AAB40447.1. U00096 Genomic DNA. Translation: AAC75839.1. AP009048 Genomic DNA. Translation: BAE76869.1.
PIR	A65062.
RefSeq	NP_417277.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P30744.
SMR	P30744. Positions 16-159.
ModBase	Search...
Protein-protein interaction databases
IntAct	P30744. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001926; EBESCP00000001926; EBESCG00000001579. EBESCT00000015574; EBESCP00000014865; EBESCG00000014634.
GeneID	947262.
GenomeReviews	Gene locus JW2768 in contig AP009048_GR. Gene locus b2797 in contig U00096_GR.
KEGG	ecj:JW2768. eco:b2797.
PATRIC	32121010. VBIEscCol129921_2897.
Organism-specific databases
EchoBASE	EB1580.
EcoGene	EG11623. sdaB.
Phylogenomic databases
eggNOG	COG1760.
GeneTree	EBGT00050000009684.
HOGENOM	HBG304565.
OMA	ENGMRIS.
PhylomeDB	P30744.
ProtClustDB	CLSK880494.
Enzyme and pathway databases
BioCyc	EcoCyc:LSERINEDEAM2-MONOMER. MetaCyc:LSERINEDEAM2-MONOMER.
Gene expression databases
Genevestigator	P30744.
Family and domain databases
InterPro	IPR004644. Fe-S_L-Ser_mono. IPR005130. Ser_deHydtase-like_asu. IPR005131. Ser_deHydtase_bsu. [Graphical view]
KO	K01752.
Pfam	PF03313. SDH_alpha. 1 hit. PF03315. SDH_beta. 1 hit. [Graphical view]
TIGRFAMs	TIGR00720. Sda_mono. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SDHM_ECOLI

Accession

Primary (citable) accession number: P30744
Secondary accession number(s): Q2MA37, Q59377

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program