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Protein

Leukocyte elastase inhibitor

Gene

SERPINB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Also functions as a potent intracellular inhibitor of granzyme H.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei343 – 344Reactive bond 12
Sitei344 – 345Reactive bond 22

GO - Molecular functioni

  • serine-type endopeptidase inhibitor activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

BioCyciZFISH:ENSG00000021355-MONOMER.
ReactomeiR-HSA-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiI04.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukocyte elastase inhibitor
Short name:
LEI
Alternative name(s):
Monocyte/neutrophil elastase inhibitor
Short name:
EI
Short name:
M/NEI
Peptidase inhibitor 2
Short name:
PI-2
Serpin B1
Gene namesi
Name:SERPINB1
Synonyms:ELANH2, MNEI, PI2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:3311. SERPINB1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi1992.
OpenTargetsiENSG00000021355.
PharmGKBiPA35046.

Polymorphism and mutation databases

DMDMi266344.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000941011 – 379Leukocyte elastase inhibitorAdd BLAST379

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei137N6-acetyllysineCombined sources1
Modified residuei177N6-acetyllysineCombined sources1
Modified residuei300PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP30740.
PaxDbiP30740.
PeptideAtlasiP30740.
PRIDEiP30740.

2D gel databases

OGPiP30740.
REPRODUCTION-2DPAGEIPI00027444.

PTM databases

iPTMnetiP30740.
PhosphoSitePlusiP30740.

Miscellaneous databases

PMAP-CutDBP30740.

Expressioni

Gene expression databases

BgeeiENSG00000021355.
CleanExiHS_SERPINB1.
ExpressionAtlasiP30740. baseline and differential.
GenevisibleiP30740. HS.

Organism-specific databases

HPAiHPA018871.
HPA052642.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi108307. 20 interactors.
IntActiP30740. 2 interactors.
MINTiMINT-4999893.
STRINGi9606.ENSP00000370115.

Structurei

Secondary structure

1379
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 22Combined sources20
Beta strandi24 – 26Combined sources3
Beta strandi28 – 30Combined sources3
Helixi32 – 43Combined sources12
Helixi48 – 57Combined sources10
Helixi60 – 62Combined sources3
Helixi66 – 77Combined sources12
Beta strandi83 – 95Combined sources13
Helixi102 – 111Combined sources10
Beta strandi116 – 119Combined sources4
Helixi121 – 139Combined sources19
Turni140 – 142Combined sources3
Beta strandi159 – 168Combined sources10
Beta strandi171 – 173Combined sources3
Turni177 – 179Combined sources3
Beta strandi181 – 188Combined sources8
Beta strandi191 – 209Combined sources19
Turni210 – 213Combined sources4
Beta strandi214 – 221Combined sources8
Beta strandi224 – 235Combined sources12
Beta strandi238 – 242Combined sources5
Helixi244 – 249Combined sources6
Helixi252 – 259Combined sources8
Helixi261 – 263Combined sources3
Beta strandi265 – 274Combined sources10
Beta strandi276 – 283Combined sources8
Helixi285 – 290Combined sources6
Helixi295 – 297Combined sources3
Turni299 – 301Combined sources3
Turni305 – 307Combined sources3
Beta strandi315 – 326Combined sources12
Beta strandi345 – 347Combined sources3
Beta strandi349 – 353Combined sources5
Beta strandi358 – 364Combined sources7
Turni365 – 368Combined sources4
Beta strandi369 – 376Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GA7X-ray2.90A/B1-379[»]
ProteinModelPortaliP30740.
SMRiP30740.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin, chymase or granzyme H, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophil elastase, proteinase-3, pancreatic elastase or PSA.

Sequence similaritiesi

Belongs to the serpin family. Ov-serpin subfamily.Curated

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiP30740.
KOiK13963.
OMAiDRKTVKM.
OrthoDBiEOG091G0ION.
PhylomeDBiP30740.
TreeFamiTF352619.

Family and domain databases

InterProiIPR015557. Serpin_B1.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PTHR11461:SF180. PTHR11461:SF180. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P30740-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEQLSSANTR FALDLFLALS ENNPAGNIFI SPFSISSAMA MVFLGTRGNT
60 70 80 90 100
AAQLSKTFHF NTVEEVHSRF QSLNADINKR GASYILKLAN RLYGEKTYNF
110 120 130 140 150
LPEFLVSTQK TYGADLASVD FQHASEDARK TINQWVKGQT EGKIPELLAS
160 170 180 190 200
GMVDNMTKLV LVNAIYFKGN WKDKFMKEAT TNAPFRLNKK DRKTVKMMYQ
210 220 230 240 250
KKKFAYGYIE DLKCRVLELP YQGEELSMVI LLPDDIEDES TGLKKIEEQL
260 270 280 290 300
TLEKLHEWTK PENLDFIEVN VSLPRFKLEE SYTLNSDLAR LGVQDLFNSS
310 320 330 340 350
KADLSGMSGA RDIFISKIVH KSFVEVNEEG TEAAAATAGI ATFCMLMPEE
360 370
NFTADHPFLF FIRHNSSGSI LFLGRFSSP
Length:379
Mass (Da):42,742
Last modified:April 1, 1993 - v1
Checksum:iBAAE08DFCBCD8CD3
GO
Isoform 2 (identifier: P30740-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-151: Missing.

Note: No experimental confirmation available.
Show »
Length:228
Mass (Da):26,073
Checksum:iEDA3D9952C365A47
GO

Sequence cautioni

The sequence BAD97090 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti137K → R in AAP35574 (Ref. 4) Curated1
Sequence conflicti149A → V in BAF84016 (PubMed:14702039).Curated1
Sequence conflicti264L → F in BAF84016 (PubMed:14702039).Curated1
Sequence conflicti272Missing AA sequence (PubMed:7578269).Curated1
Sequence conflicti329E → K in BAF84016 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05194582A → V.Corresponds to variant rs34825616dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0565111 – 151Missing in isoform 2. 1 PublicationAdd BLAST151

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93056 mRNA. No translation available.
AF053630 Genomic DNA. Translation: AAC31394.1.
AK291327 mRNA. Translation: BAF84016.1.
AK298044 mRNA. Translation: BAG60342.1.
BT006928 mRNA. Translation: AAP35574.1.
AK223370 mRNA. Translation: BAD97090.1. Different initiation.
AL139092 Genomic DNA. Translation: CAH73667.1.
BC009015 mRNA. Translation: AAH09015.1.
CCDSiCCDS4477.1. [P30740-1]
PIRiS27383.
RefSeqiNP_109591.1. NM_030666.3. [P30740-1]
XP_011512635.1. XM_011514333.1. [P30740-1]
XP_011512636.1. XM_011514334.2. [P30740-1]
UniGeneiHs.381167.

Genome annotation databases

EnsembliENST00000380739; ENSP00000370115; ENSG00000021355. [P30740-1]
GeneIDi1992.
KEGGihsa:1992.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93056 mRNA. No translation available.
AF053630 Genomic DNA. Translation: AAC31394.1.
AK291327 mRNA. Translation: BAF84016.1.
AK298044 mRNA. Translation: BAG60342.1.
BT006928 mRNA. Translation: AAP35574.1.
AK223370 mRNA. Translation: BAD97090.1. Different initiation.
AL139092 Genomic DNA. Translation: CAH73667.1.
BC009015 mRNA. Translation: AAH09015.1.
CCDSiCCDS4477.1. [P30740-1]
PIRiS27383.
RefSeqiNP_109591.1. NM_030666.3. [P30740-1]
XP_011512635.1. XM_011514333.1. [P30740-1]
XP_011512636.1. XM_011514334.2. [P30740-1]
UniGeneiHs.381167.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GA7X-ray2.90A/B1-379[»]
ProteinModelPortaliP30740.
SMRiP30740.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108307. 20 interactors.
IntActiP30740. 2 interactors.
MINTiMINT-4999893.
STRINGi9606.ENSP00000370115.

Protein family/group databases

MEROPSiI04.006.

PTM databases

iPTMnetiP30740.
PhosphoSitePlusiP30740.

Polymorphism and mutation databases

DMDMi266344.

2D gel databases

OGPiP30740.
REPRODUCTION-2DPAGEIPI00027444.

Proteomic databases

EPDiP30740.
PaxDbiP30740.
PeptideAtlasiP30740.
PRIDEiP30740.

Protocols and materials databases

DNASUi1992.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000380739; ENSP00000370115; ENSG00000021355. [P30740-1]
GeneIDi1992.
KEGGihsa:1992.

Organism-specific databases

CTDi1992.
DisGeNETi1992.
GeneCardsiSERPINB1.
HGNCiHGNC:3311. SERPINB1.
HPAiHPA018871.
HPA052642.
MIMi130135. gene.
neXtProtiNX_P30740.
OpenTargetsiENSG00000021355.
PharmGKBiPA35046.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiP30740.
KOiK13963.
OMAiDRKTVKM.
OrthoDBiEOG091G0ION.
PhylomeDBiP30740.
TreeFamiTF352619.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000021355-MONOMER.
ReactomeiR-HSA-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiSERPINB1. human.
GeneWikiiSERPINB1.
GenomeRNAii1992.
PMAP-CutDBP30740.
PROiP30740.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000021355.
CleanExiHS_SERPINB1.
ExpressionAtlasiP30740. baseline and differential.
GenevisibleiP30740. HS.

Family and domain databases

InterProiIPR015557. Serpin_B1.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PTHR11461:SF180. PTHR11461:SF180. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiILEU_HUMAN
AccessioniPrimary (citable) accession number: P30740
Secondary accession number(s): A8K5L2
, B4DNT0, Q53FB9, Q5W0E1, Q9UDF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 30, 2016
This is version 164 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.