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P30728 (DRD3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D(3) dopamine receptor
Alternative name(s):
Dopamine D3 receptor
Gene names
Name:Drd3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation By similarity.

Subunit structure

Interacts with CLIC6, GRK4 and PALM By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Phosphorylated by GRK4 By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor internalization

Inferred from electronic annotation. Source: Ensembl

adenylate cyclase-activating dopamine receptor signaling pathway

Inferred from direct assay PubMed 16386234. Source: MGI

adenylate cyclase-inhibiting dopamine receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

arachidonic acid secretion

Inferred from electronic annotation. Source: Ensembl

behavioral response to cocaine

Inferred from mutant phenotype PubMed 10432116. Source: BHF-UCL

cellular calcium ion homeostasis

Inferred from electronic annotation. Source: Ensembl

circadian regulation of gene expression

Inferred from mutant phenotype PubMed 15878801. Source: MGI

gastric emptying

Inferred from electronic annotation. Source: Ensembl

locomotory behavior

Inferred from mutant phenotype PubMed 12044470PubMed 8700864PubMed 9402626. Source: MGI

musculoskeletal movement, spinal reflex action

Inferred from mutant phenotype PubMed 15601940. Source: MGI

negative regulation of adenylate cyclase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of blood pressure

Inferred from mutant phenotype PubMed 9691085. Source: MGI

negative regulation of dopamine receptor signaling pathway

Inferred from genetic interaction PubMed 12097513. Source: MGI

negative regulation of oligodendrocyte differentiation

Inferred from direct assay PubMed 9651217. Source: MGI

negative regulation of protein kinase B signaling

Inferred from mutant phenotype PubMed 17251429. Source: MGI

negative regulation of protein secretion

Inferred from electronic annotation. Source: Ensembl

negative regulation of sodium:proton antiporter activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of dopamine receptor signaling pathway

Inferred from mutant phenotype PubMed 15016423. Source: MGI

positive regulation of mitosis

Inferred from electronic annotation. Source: Ensembl

positive regulation of renal sodium excretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

prepulse inhibition

Inferred from electronic annotation. Source: Ensembl

regulation of blood volume by renin-angiotensin

Inferred from mutant phenotype PubMed 9691085. Source: MGI

regulation of circadian sleep/wake cycle, sleep

Inferred from electronic annotation. Source: Ensembl

regulation of dopamine secretion

Inferred from mutant phenotype PubMed 12044470PubMed 9482807. Source: MGI

regulation of lipid metabolic process

Inferred from mutant phenotype PubMed 15084447. Source: MGI

regulation of locomotion involved in locomotory behavior

Inferred from electronic annotation. Source: Ensembl

regulation of multicellular organism growth

Inferred from mutant phenotype PubMed 15084447. Source: MGI

response to amphetamine

Inferred from genetic interaction PubMed 12097513. Source: MGI

response to cocaine

Inferred from mutant phenotype PubMed 10943692. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from mutant phenotype PubMed 11988344. Source: MGI

response to histamine

Inferred from electronic annotation. Source: Ensembl

response to morphine

Inferred from mutant phenotype PubMed 12574430. Source: MGI

synaptic transmission, dopaminergic

Inferred from electronic annotation. Source: Ensembl

visual learning

Inferred from mutant phenotype PubMed 12097513. Source: MGI

   Cellular_componentapical part of cell

Inferred from electronic annotation. Source: Ensembl

cell projection

Inferred from electronic annotation. Source: Ensembl

endocytic vesicle

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Inferred from electronic annotation. Source: InterPro

membrane

Inferred from direct assay PubMed 9651217. Source: MGI

plasma membrane

Inferred from direct assay PubMed 16386234PubMed 9651217. Source: MGI

   Molecular_functiondopamine binding

Inferred from electronic annotation. Source: Ensembl

dopamine neurotransmitter receptor activity, coupled via Gi/Go

Inferred from direct assay PubMed 7566118Ref.1PubMed 8700864PubMed 8836575. Source: MGI

drug binding

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 16386234. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform D3Long (identifier: P30728-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform D3Short (identifier: P30728-2)

The sequence of this isoform differs from the canonical sequence as follows:
     268-288: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446D(3) dopamine receptor
PRO_0000069398

Regions

Topological domain1 – 3232Extracellular By similarity
Transmembrane33 – 5523Helical; Name=1; By similarity
Topological domain56 – 6510Cytoplasmic By similarity
Transmembrane66 – 8823Helical; Name=2; By similarity
Topological domain89 – 10416Extracellular By similarity
Transmembrane105 – 12622Helical; Name=3; By similarity
Topological domain127 – 14923Cytoplasmic By similarity
Transmembrane150 – 17021Helical; Name=4; By similarity
Topological domain171 – 18717Extracellular By similarity
Transmembrane188 – 21225Helical; Name=5; By similarity
Topological domain213 – 375163Cytoplasmic By similarity
Transmembrane376 – 39722Helical; Name=6; By similarity
Topological domain398 – 41215Extracellular By similarity
Transmembrane413 – 43422Helical; Name=7; By similarity
Topological domain435 – 44612Cytoplasmic By similarity
Region189 – 1968Agonist binding By similarity
Region388 – 3969Agonist binding By similarity

Sites

Binding site1101Agonist By similarity

Amino acid modifications

Glycosylation121N-linked (GlcNAc...) Potential
Glycosylation191N-linked (GlcNAc...) Potential
Glycosylation971N-linked (GlcNAc...) Potential
Glycosylation1731N-linked (GlcNAc...) Potential
Disulfide bond103 ↔ 181 By similarity
Disulfide bond401 ↔ 404 By similarity

Natural variations

Alternative sequence268 – 28821Missing in isoform D3Short.
VSP_001873

Sequences

Sequence LengthMass (Da)Tools
Isoform D3Long [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: B391C2D493B58242

FASTA44649,669
        10         20         30         40         50         60 
MAPLSQISSH INSTCGAENS TGVNRARPHA YYALSYCALI LAIIFGNGLV CAAVLRERAL 

        70         80         90        100        110        120 
QTTTNYLVVS LAVADLLVAT LVMPWVVYLE VTGGVWNFSR ICCDVFVTLD VMMCTASILN 

       130        140        150        160        170        180 
LCAISIDRYT AVVMPVHYQH GTGQSSCRRV ALMITAVWVL AFAVSCPLLF GFNTTGDPSI 

       190        200        210        220        230        240 
CSISNPDFVI YSSVVSFYVP FGVTVLVYAR IYMVLRQRRR KRILTRQNSQ CISIRPGFPQ 

       250        260        270        280        290        300 
QSSCLRLHPI RQFSIRARFL SDATGQMEHI EDKPYPQKCQ DPLLSHLQPL SPGQTHGELK 

       310        320        330        340        350        360 
RYYSICQDTA LRHPNFEGGG GMSQVERTRN SLSPTMAPKL SLEVRKLSNG RLSTSLKLGP 

       370        380        390        400        410        420 
LQPRGVPLRE KKATQMVVIV LGAFIVCWLP FFLTHVLNTH CQACHVSPEL YRATTWLGYV 

       430        440 
NSALNPVIYT TFNIEFRKAF LKILSC 

« Hide

Isoform D3Short [UniParc].

Checksum: E4C0EDFB345BAE87
Show »

FASTA42547,168

References

[1]"A novel short isoform of the D3 dopamine receptor generated by alternative splicing in the third cytoplasmic loop."
Fishburn C.S., Belleli D., David C., Carmon S., Fuchs S.
J. Biol. Chem. 268:5872-5878(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS D3LONG AND D3SHORT).
Strain: BALB/c.
[2]"Molecular cloning and characterization of the mouse dopamine D3 receptor gene: an additional intron and an mRNA variant."
Fu D., Skryabin B.V., Brosius J., Robakis N.K.
DNA Cell Biol. 14:485-492(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X67274 mRNA. Translation: CAA47691.1.
S78580 expand/collapse EMBL AC list , S78574, S78638, S78577, S78578, S78579, S78639 Genomic DNA. Translation: AAB35066.2.
CCDSCCDS28179.1. [P30728-1]
PIRI48322.
RefSeqNP_031903.1. NM_007877.1. [P30728-1]
UniGeneMm.439735.

3D structure databases

ProteinModelPortalP30728.
SMRP30728. Positions 32-224, 351-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199307. 1 interaction.
STRING10090.ENSMUSP00000023390.

Chemistry

BindingDBP30728.
ChEMBLCHEMBL2111359.
GuidetoPHARMACOLOGY216.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP30728.

Proteomic databases

PaxDbP30728.
PRIDEP30728.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023390; ENSMUSP00000023390; ENSMUSG00000022705. [P30728-1]
GeneID13490.
KEGGmmu:13490.
UCSCuc007zgi.1. mouse. [P30728-1]

Organism-specific databases

CTD1814.
MGIMGI:94925. Drd3.

Phylogenomic databases

eggNOGNOG309657.
HOGENOMHOG000239242.
HOVERGENHBG106962.
InParanoidP30728.
KOK04146.
OMALHPIRQF.
OrthoDBEOG769ZMG.
PhylomeDBP30728.
TreeFamTF334382.

Gene expression databases

BgeeP30728.
CleanExMM_DRD3.
GenevestigatorP30728.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR001620. Dopamine_D3_rcpt.
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24249:SF38. PTHR24249:SF38. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00568. DOPAMINED3R.
PR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio284005.
PROP30728.
SOURCESearch...

Entry information

Entry nameDRD3_MOUSE
AccessionPrimary (citable) accession number: P30728
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries