P30728 (DRD3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D(3) dopamine receptor

Alternative name(s):
Dopamine D3 receptor

Gene names

Name:

Drd3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation By similarity.
Subunit structure	Interacts with CLIC6, GRK4 and PALM By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Phosphorylated by GRK4 By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor internalization Inferred from electronic annotation. Source: Compara adenylate cyclase-activating dopamine receptor signaling pathway Inferred from direct assay PubMed 16386234. Source: MGI adenylate cyclase-inhibiting dopamine receptor signaling pathway Inferred from electronic annotation. Source: Compara arachidonic acid secretion Inferred from electronic annotation. Source: Compara behavioral response to cocaine Inferred from mutant phenotype PubMed 10432116. Source: BHF-UCL cellular calcium ion homeostasis Inferred from electronic annotation. Source: Compara circadian regulation of gene expression Inferred from mutant phenotype PubMed 15878801. Source: MGI locomotory behavior Inferred from mutant phenotype PubMed 12044470PubMed 8700864PubMed 9402626. Source: MGI musculoskeletal movement, spinal reflex action Inferred from mutant phenotype PubMed 15601940. Source: MGI negative regulation of adenylate cyclase activity Inferred from electronic annotation. Source: Compara negative regulation of blood pressure Inferred from mutant phenotype PubMed 9691085. Source: MGI negative regulation of dopamine receptor signaling pathway Inferred from genetic interaction PubMed 12097513. Source: MGI negative regulation of oligodendrocyte differentiation Inferred from direct assay PubMed 9651217. Source: MGI negative regulation of protein kinase B signaling cascade Inferred from mutant phenotype PubMed 17251429. Source: MGI negative regulation of protein secretion Inferred from electronic annotation. Source: Compara negative regulation of sodium:hydrogen antiporter activity Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of dopamine receptor signaling pathway Inferred from mutant phenotype PubMed 15016423. Source: MGI positive regulation of mitosis Inferred from electronic annotation. Source: Compara positive regulation of renal sodium excretion Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara prepulse inhibition Inferred from electronic annotation. Source: Compara regulation of blood volume by renin-angiotensin Inferred from mutant phenotype PubMed 9691085. Source: MGI regulation of circadian sleep/wake cycle, sleep Inferred from electronic annotation. Source: Compara regulation of dopamine secretion Inferred from mutant phenotype PubMed 12044470PubMed 9482807. Source: MGI regulation of lipid metabolic process Inferred from mutant phenotype PubMed 15084447. Source: MGI regulation of locomotion Inferred from electronic annotation. Source: Compara regulation of multicellular organism growth Inferred from mutant phenotype PubMed 15084447. Source: MGI response to amphetamine Inferred from genetic interaction PubMed 12097513. Source: MGI response to cocaine Inferred from mutant phenotype PubMed 10943692. Source: MGI response to drug Inferred from electronic annotation. Source: Compara response to ethanol Inferred from mutant phenotype PubMed 11988344. Source: MGI response to histamine Inferred from electronic annotation. Source: Compara response to morphine Inferred from mutant phenotype PubMed 12574430. Source: MGI synaptic transmission, dopaminergic Inferred from electronic annotation. Source: Compara visual learning Inferred from mutant phenotype PubMed 12097513. Source: MGI
Cellular_component	apical part of cell Inferred from electronic annotation. Source: Compara cell projection Inferred from electronic annotation. Source: Compara endocytic vesicle Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 16386234PubMed 9651217. Source: MGI
Molecular_function	dopamine binding Inferred from electronic annotation. Source: Compara dopamine neurotransmitter receptor activity, coupled via Gi/Go Inferred from direct assay PubMed 7566118Ref.1PubMed 8700864PubMed 8836575. Source: MGI drug binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform D3Long (identifier: P30728-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform D3Short (identifier: P30728-2)

The sequence of this isoform differs from the canonical sequence as follows:
     268-288: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	D(3) dopamine receptor		PRO_0000069398
Regions
	Topological domain	1 – 32	32	Extracellular By similarity
	Transmembrane	33 – 55	23	Helical; Name=1; By similarity
	Topological domain	56 – 65	10	Cytoplasmic By similarity
	Transmembrane	66 – 88	23	Helical; Name=2; By similarity
	Topological domain	89 – 104	16	Extracellular By similarity
	Transmembrane	105 – 126	22	Helical; Name=3; By similarity
	Topological domain	127 – 149	23	Cytoplasmic By similarity
	Transmembrane	150 – 170	21	Helical; Name=4; By similarity
	Topological domain	171 – 187	17	Extracellular By similarity
	Transmembrane	188 – 212	25	Helical; Name=5; By similarity
	Topological domain	213 – 375	163	Cytoplasmic By similarity
	Transmembrane	376 – 397	22	Helical; Name=6; By similarity
	Topological domain	398 – 412	15	Extracellular By similarity
	Transmembrane	413 – 434	22	Helical; Name=7; By similarity
	Topological domain	435 – 446	12	Cytoplasmic By similarity
	Region	189 – 196	8	Agonist binding By similarity
	Region	388 – 396	9	Agonist binding By similarity
Sites
	Binding site	110	1	Agonist By similarity
Amino acid modifications
	Glycosylation	12	1	N-linked (GlcNAc...) Potential
	Glycosylation	19	1	N-linked (GlcNAc...) Potential
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Disulfide bond	103 ↔ 181		By similarity
	Disulfide bond	401 ↔ 404		By similarity
Natural variations
	Alternative sequence	268 – 288	21	Missing in isoform D3Short.		VSP_001873

Sequences

	Sequence		Length	Mass (Da)
	Isoform D3Long [UniParc]. Last modified April 1, 1993. Version 1. Checksum: B391C2D493B58242	FASTA	446	49,669
	10 20 30 40 50 60 MAPLSQISSH INSTCGAENS TGVNRARPHA YYALSYCALI LAIIFGNGLV CAAVLRERAL 70 80 90 100 110 120 QTTTNYLVVS LAVADLLVAT LVMPWVVYLE VTGGVWNFSR ICCDVFVTLD VMMCTASILN 130 140 150 160 170 180 LCAISIDRYT AVVMPVHYQH GTGQSSCRRV ALMITAVWVL AFAVSCPLLF GFNTTGDPSI 190 200 210 220 230 240 CSISNPDFVI YSSVVSFYVP FGVTVLVYAR IYMVLRQRRR KRILTRQNSQ CISIRPGFPQ 250 260 270 280 290 300 QSSCLRLHPI RQFSIRARFL SDATGQMEHI EDKPYPQKCQ DPLLSHLQPL SPGQTHGELK 310 320 330 340 350 360 RYYSICQDTA LRHPNFEGGG GMSQVERTRN SLSPTMAPKL SLEVRKLSNG RLSTSLKLGP 370 380 390 400 410 420 LQPRGVPLRE KKATQMVVIV LGAFIVCWLP FFLTHVLNTH CQACHVSPEL YRATTWLGYV 430 440 NSALNPVIYT TFNIEFRKAF LKILSC
	Isoform D3Short [UniParc]. Checksum: E4C0EDFB345BAE87	FASTA	425	47,168
	10 20 30 40 50 60 MAPLSQISSH INSTCGAENS TGVNRARPHA YYALSYCALI LAIIFGNGLV CAAVLRERAL 70 80 90 100 110 120 QTTTNYLVVS LAVADLLVAT LVMPWVVYLE VTGGVWNFSR ICCDVFVTLD VMMCTASILN 130 140 150 160 170 180 LCAISIDRYT AVVMPVHYQH GTGQSSCRRV ALMITAVWVL AFAVSCPLLF GFNTTGDPSI 190 200 210 220 230 240 CSISNPDFVI YSSVVSFYVP FGVTVLVYAR IYMVLRQRRR KRILTRQNSQ CISIRPGFPQ 250 260 270 280 290 300 QSSCLRLHPI RQFSIRARFL SDATGQMPLS PGQTHGELKR YYSICQDTAL RHPNFEGGGG 310 320 330 340 350 360 MSQVERTRNS LSPTMAPKLS LEVRKLSNGR LSTSLKLGPL QPRGVPLREK KATQMVVIVL 370 380 390 400 410 420 GAFIVCWLPF FLTHVLNTHC QACHVSPELY RATTWLGYVN SALNPVIYTT FNIEFRKAFL KILSC

References

[1]	"A novel short isoform of the D3 dopamine receptor generated by alternative splicing in the third cytoplasmic loop." Fishburn C.S., Belleli D., David C., Carmon S., Fuchs S. J. Biol. Chem. 268:5872-5878(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS D3LONG AND D3SHORT). Strain: BALB/c.
[2]	"Molecular cloning and characterization of the mouse dopamine D3 receptor gene: an additional intron and an mRNA variant." Fu D., Skryabin B.V., Brosius J., Robakis N.K. DNA Cell Biol. 14:485-492(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67274 mRNA. Translation: CAA47691.1. S78580 , S78574, S78638, S78577, S78578, S78579, S78639 Genomic DNA. Translation: AAB35066.2.
IPI	IPI00133472. IPI00221401.
PIR	I48322.
RefSeq	NP_031903.1. NM_007877.1.
UniGene	Mm.439735.
3D structure databases
ProteinModelPortal	P30728.
SMR	P30728. Positions 32-224, 351-446.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000023390.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P30728.
Proteomic databases
PaxDb	P30728.
PRIDE	P30728.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023390; ENSMUSP00000023390; ENSMUSG00000022705.
GeneID	13490.
KEGG	mmu:13490.
Organism-specific databases
CTD	1814.
MGI	MGI:94925. Drd3.
Phylogenomic databases
eggNOG	NOG309657.
HOGENOM	HOG000239242.
HOVERGEN	HBG106962.
InParanoid	P30728.
KO	K04146.
OMA	EGRTRNS.
OrthoDB	EOG47M1Z5.
Gene expression databases
ArrayExpress	P30728.
Bgee	P30728.
CleanEx	MM_DRD3.
Genevestigator	P30728.
GermOnline	ENSMUSG00000022705. Mus musculus.
Family and domain databases
InterPro	IPR001620. Dopa_D3_rcpt. IPR000929. Dopamine_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00568. DOPAMINED3R. PR00242. DOPAMINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P30728.
ChEMBL	CHEMBL3441.
NextBio	284005.
SOURCE	Search...

Entry information

Entry name

DRD3_MOUSE

Accession

Primary (citable) accession number: P30728

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program