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Protein

D(3) dopamine receptor

Gene

Drd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101AgonistBy similarity

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-activating dopamine receptor signaling pathway Source: MGI
  • adenylate cyclase-inhibiting dopamine receptor signaling pathway Source: MGI
  • arachidonic acid secretion Source: MGI
  • behavioral response to cocaine Source: BHF-UCL
  • cellular calcium ion homeostasis Source: MGI
  • circadian regulation of gene expression Source: MGI
  • gastric emptying Source: Ensembl
  • G-protein coupled receptor internalization Source: MGI
  • G-protein coupled receptor signaling pathway Source: MGI
  • locomotory behavior Source: MGI
  • musculoskeletal movement, spinal reflex action Source: MGI
  • negative regulation of adenylate cyclase activity Source: MGI
  • negative regulation of blood pressure Source: MGI
  • negative regulation of dopamine receptor signaling pathway Source: MGI
  • negative regulation of oligodendrocyte differentiation Source: MGI
  • negative regulation of protein kinase B signaling Source: MGI
  • negative regulation of protein secretion Source: MGI
  • negative regulation of sodium:proton antiporter activity Source: Ensembl
  • negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  • positive regulation of cell proliferation Source: Ensembl
  • positive regulation of cytokinesis Source: MGI
  • positive regulation of dopamine receptor signaling pathway Source: MGI
  • positive regulation of mitotic nuclear division Source: Ensembl
  • positive regulation of renal sodium excretion Source: Ensembl
  • positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  • prepulse inhibition Source: MGI
  • regulation of blood volume by renin-angiotensin Source: MGI
  • regulation of cAMP metabolic process Source: MGI
  • regulation of circadian sleep/wake cycle, sleep Source: Ensembl
  • regulation of dopamine secretion Source: MGI
  • regulation of lipid metabolic process Source: MGI
  • regulation of locomotion involved in locomotory behavior Source: Ensembl
  • regulation of multicellular organism growth Source: MGI
  • response to amphetamine Source: MGI
  • response to cocaine Source: MGI
  • response to drug Source: Ensembl
  • response to ethanol Source: MGI
  • response to histamine Source: MGI
  • response to morphine Source: MGI
  • sensory perception of chemical stimulus Source: GO_Central
  • synaptic transmission, dopaminergic Source: Ensembl
  • visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_294218. Dopamine receptors.
REACT_331048. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
D(3) dopamine receptor
Alternative name(s):
Dopamine D3 receptor
Gene namesi
Name:Drd3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:94925. Drd3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232ExtracellularBy similarityAdd
BLAST
Transmembranei33 – 5523Helical; Name=1By similarityAdd
BLAST
Topological domaini56 – 6510CytoplasmicBy similarity
Transmembranei66 – 8823Helical; Name=2By similarityAdd
BLAST
Topological domaini89 – 10416ExtracellularBy similarityAdd
BLAST
Transmembranei105 – 12622Helical; Name=3By similarityAdd
BLAST
Topological domaini127 – 14923CytoplasmicBy similarityAdd
BLAST
Transmembranei150 – 17021Helical; Name=4By similarityAdd
BLAST
Topological domaini171 – 18717ExtracellularBy similarityAdd
BLAST
Transmembranei188 – 21225Helical; Name=5By similarityAdd
BLAST
Topological domaini213 – 375163CytoplasmicBy similarityAdd
BLAST
Transmembranei376 – 39722Helical; Name=6By similarityAdd
BLAST
Topological domaini398 – 41215ExtracellularBy similarityAdd
BLAST
Transmembranei413 – 43422Helical; Name=7By similarityAdd
BLAST
Topological domaini435 – 44612CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • apical part of cell Source: Ensembl
  • cell projection Source: Ensembl
  • endocytic vesicle Source: Ensembl
  • integral component of plasma membrane Source: MGI
  • membrane Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446D(3) dopamine receptorPRO_0000069398Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi12 – 121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi19 – 191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi103 ↔ 181PROSITE-ProRule annotation
Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi401 ↔ 404PROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by GRK4.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP30728.
PRIDEiP30728.

PTM databases

PhosphoSiteiP30728.

Expressioni

Gene expression databases

BgeeiP30728.
CleanExiMM_DRD3.
ExpressionAtlasiP30728. baseline.
GenevisibleiP30728. MM.

Interactioni

Subunit structurei

Interacts with CLIC6, GRK4 and PALM.By similarity

Protein-protein interaction databases

BioGridi199307. 1 interaction.
STRINGi10090.ENSMUSP00000023390.

Structurei

3D structure databases

ProteinModelPortaliP30728.
SMRiP30728. Positions 30-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1968Agonist bindingBy similarity
Regioni388 – 3969Agonist bindingBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG309657.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP30728.
KOiK04146.
OMAiLHPIRQF.
OrthoDBiEOG769ZMG.
PhylomeDBiP30728.
TreeFamiTF334382.

Family and domain databases

InterProiIPR001620. Dopamine_D3_rcpt.
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24249:SF38. PTHR24249:SF38. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00568. DOPAMINED3R.
PR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform D3Long (identifier: P30728-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPLSQISSH INSTCGAENS TGVNRARPHA YYALSYCALI LAIIFGNGLV
60 70 80 90 100
CAAVLRERAL QTTTNYLVVS LAVADLLVAT LVMPWVVYLE VTGGVWNFSR
110 120 130 140 150
ICCDVFVTLD VMMCTASILN LCAISIDRYT AVVMPVHYQH GTGQSSCRRV
160 170 180 190 200
ALMITAVWVL AFAVSCPLLF GFNTTGDPSI CSISNPDFVI YSSVVSFYVP
210 220 230 240 250
FGVTVLVYAR IYMVLRQRRR KRILTRQNSQ CISIRPGFPQ QSSCLRLHPI
260 270 280 290 300
RQFSIRARFL SDATGQMEHI EDKPYPQKCQ DPLLSHLQPL SPGQTHGELK
310 320 330 340 350
RYYSICQDTA LRHPNFEGGG GMSQVERTRN SLSPTMAPKL SLEVRKLSNG
360 370 380 390 400
RLSTSLKLGP LQPRGVPLRE KKATQMVVIV LGAFIVCWLP FFLTHVLNTH
410 420 430 440
CQACHVSPEL YRATTWLGYV NSALNPVIYT TFNIEFRKAF LKILSC
Length:446
Mass (Da):49,669
Last modified:April 1, 1993 - v1
Checksum:iB391C2D493B58242
GO
Isoform D3Short (identifier: P30728-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     268-288: Missing.

Show »
Length:425
Mass (Da):47,168
Checksum:iE4C0EDFB345BAE87
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei268 – 28821Missing in isoform D3Short. 1 PublicationVSP_001873Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67274 mRNA. Translation: CAA47691.1.
S78580
, S78574, S78638, S78577, S78578, S78579, S78639 Genomic DNA. Translation: AAB35066.2.
CCDSiCCDS28179.1. [P30728-1]
PIRiI48322.
RefSeqiNP_031903.1. NM_007877.1. [P30728-1]
UniGeneiMm.439735.

Genome annotation databases

EnsembliENSMUST00000023390; ENSMUSP00000023390; ENSMUSG00000022705. [P30728-1]
GeneIDi13490.
KEGGimmu:13490.
UCSCiuc007zgi.1. mouse. [P30728-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67274 mRNA. Translation: CAA47691.1.
S78580
, S78574, S78638, S78577, S78578, S78579, S78639 Genomic DNA. Translation: AAB35066.2.
CCDSiCCDS28179.1. [P30728-1]
PIRiI48322.
RefSeqiNP_031903.1. NM_007877.1. [P30728-1]
UniGeneiMm.439735.

3D structure databases

ProteinModelPortaliP30728.
SMRiP30728. Positions 30-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199307. 1 interaction.
STRINGi10090.ENSMUSP00000023390.

Chemistry

ChEMBLiCHEMBL2111359.
GuidetoPHARMACOLOGYi216.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP30728.

Proteomic databases

PaxDbiP30728.
PRIDEiP30728.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023390; ENSMUSP00000023390; ENSMUSG00000022705. [P30728-1]
GeneIDi13490.
KEGGimmu:13490.
UCSCiuc007zgi.1. mouse. [P30728-1]

Organism-specific databases

CTDi1814.
MGIiMGI:94925. Drd3.

Phylogenomic databases

eggNOGiNOG309657.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP30728.
KOiK04146.
OMAiLHPIRQF.
OrthoDBiEOG769ZMG.
PhylomeDBiP30728.
TreeFamiTF334382.

Enzyme and pathway databases

ReactomeiREACT_294218. Dopamine receptors.
REACT_331048. G alpha (i) signalling events.

Miscellaneous databases

NextBioi284005.
PROiP30728.
SOURCEiSearch...

Gene expression databases

BgeeiP30728.
CleanExiMM_DRD3.
ExpressionAtlasiP30728. baseline.
GenevisibleiP30728. MM.

Family and domain databases

InterProiIPR001620. Dopamine_D3_rcpt.
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24249:SF38. PTHR24249:SF38. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00568. DOPAMINED3R.
PR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A novel short isoform of the D3 dopamine receptor generated by alternative splicing in the third cytoplasmic loop."
    Fishburn C.S., Belleli D., David C., Carmon S., Fuchs S.
    J. Biol. Chem. 268:5872-5878(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS D3LONG AND D3SHORT).
    Strain: BALB/c.
  2. "Molecular cloning and characterization of the mouse dopamine D3 receptor gene: an additional intron and an mRNA variant."
    Fu D., Skryabin B.V., Brosius J., Robakis N.K.
    DNA Cell Biol. 14:485-492(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiDRD3_MOUSE
AccessioniPrimary (citable) accession number: P30728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 24, 2015
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.