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Reviewed, UniProtKB/Swiss-Prot P30724 (G3PA_GRAVE)

Last modified June 16, 2009. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glyceraldehyde-3-phosphate dehydrogenase, chloroplastic
    EC=1.2.1.13
Alternative name(s):
    NADP-dependent glyceraldehydephosphate dehydrogenase
Gene names
Name: GAPA
OrganismGracilaria verrucosa (Red alga)
Taxonomic identifier2777 [NCBI]
Taxonomic lineageEukaryota › Rhodophyta › Florideophyceae › Gracilariales › Gracilariaceae › Gracilaria

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Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathway

Carbohydrate biosynthesis; Calvin cycle.

Subunit structure

Homotetramer By similarity.

Subcellular location

Plastid › chloroplast.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7878Chloroplast Potential
Chain79 – 416338Glyceraldehyde-3-phosphate dehydrogenase, chloroplastic
PRO_0000010420

Regions

Nucleotide binding90 – 912NADP By similarity
Region232 – 2343Glyceraldehyde 3-phosphate binding By similarity
Region291 – 2922Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site2331Nucleophile By similarity
Binding site1141NADP By similarity
Binding site1581NADP; via carbonyl oxygen By similarity
Binding site2631Glyceraldehyde 3-phosphate By similarity
Binding site2781Glyceraldehyde 3-phosphate By similarity
Binding site3141Glyceraldehyde 3-phosphate By similarity
Binding site3961NADP By similarity
Site2601Activates thiol group during catalysis By similarity

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Sequences

Sequence LengthMass (Da)Tools
P30724-1 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 33B9437E84230BD8

FASTA41644,338
        10         20         30         40         50         60 
MAFVAPVSSV FSTSSKSAVC SGRSSFAQFS GLKKVNNTAR LQTAEQGSAF GGVSDANDAF 

        70         80         90        100        110        120 
FNAVNTMGAP ARTSNAPSMK VRVAINGFGR IGRNFIRCWA GRTDSNMDVV CINDTSGVKT 

       130        140        150        160        170        180 
ASHLLKYDSI LGTFDSDVVA GEDSITVDGK TIKVVSNRNP LELPWKEMEI DIVVEATGVF 

       190        200        210        220        230        240 
VDAVGAGKHI QAGAKKVLIT APGKGEGVGT FVVGVNDHLY SHDKFDIVSN ASCTTNCMAP 

       250        260        270        280        290        300 
FMKVLDDEFG VVRGMMTTTH SYTGDQRLLD AGHRDLRRAR SAALNIVPTT TGAAKAVALV 

       310        320        330        340        350        360 
VPTLAGKLNG IALRVPTPNV SVCDVVMQVS KKTFKEEVNG ALLKAANGSM KGIIKYSDEP 

       370        380        390        400        410 
LVSCDYRGTD ESTIIDSSLT MVMGDDMLKV VAWYDNEWGY SQRVVDLGEV MASQWK

« Hide

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References

[1]"cDNA cloning and characterization of the nuclear gene encoding chloroplast glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa."
Zhou Y.H., Ragan M.A.
Curr. Genet. 23:483-489(1993) [PubMed: 7916671] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Norway.
[2]"Cloning and characterization of the nuclear gene encoding plastid glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa."
Zhou Y.H., Ragan M.A.
Curr. Genet. 26:79-86(1994) [PubMed: 7954900] [Abstract]
Cited for: SEQUENCE REVISION.

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Cross-references

Sequence databases

Z15102 mRNA. Translation: CAA78811.1.
L22011 Genomic DNA. Translation: AAA33355.1.
PIRS45484.

3D structure databases

HSSPHSSP built from PDB template 1NBO based on UniProtKB P19866.
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.2.1.13. 1477.

Family and domain databases

InterProIPR000173. GlycerAld_3-P_DH.
IPR006424. Glyceraldehyde-3-P_DH_1.
[Graphical view]
PANTHERPTHR10836. GAP_DH. 1 hit.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSPR00078. G3PDHDRGNASE.
TIGRFAMsTIGR01534. GAPDH-I. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameG3PA_GRAVE
AccessionPrimary (citable) accession number: P30724
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents