P30715 (AT1B1_BUFMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Sodium/potassium-transporting ATPase subunit beta-1 Alternative name(s): Sodium/potassium-dependent ATPase beta-1 subunit
Organism	Bufo marinus (Giant toad) (Cane toad)
Taxonomic identifier	8386 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Neobatrachia › Hyloidea › Bufonidae › Bufo › Rhinella

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of this glycoprotein is not known. Some specific sequence of the beta subunit can modulate the activation of the Na,K-pump by extracellular potassium ions.
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Abundantly in kidney, at a lower extent in bladder, colon, eye and testis. Low levels in brain, heart, spleen and liver.
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sodium:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Sodium/potassium-transporting ATPase subunit beta-1		PRO_0000219117
Regions
	Topological domain	1 – 32	32	Cytoplasmic Potential
	Transmembrane	33 – 53	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	54 – 303	250	Extracellular Potential
Amino acid modifications
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	164	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Glycosylation	264	1	N-linked (GlcNAc...) Potential
	Disulfide bond	127 ↔ 150		By similarity
	Disulfide bond	160 ↔ 176		By similarity
	Disulfide bond	212 ↔ 275		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30715 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 3A4493D00F25805D	FASTA	303	34,533
	10 20 30 40 50 60 MARDKNKEND GSWKKFLWDP EKKEFMGRTG SSWFKILLFY LVFYGCLAGI FIGTIQVLLL 70 80 90 100 110 120 TLSIYEPKYQ DRVAPPGLTQ VPRAVKAEIS FTVGNPSTYE DYVTSLSNFL NQYNSSKQDN 130 140 150 160 170 180 LALFEDCGDK PKGYIDRGAI SPDHGTKRSC QFKREWLGEC SGLNDTTFGF NEGKPCLIVK 190 200 210 220 230 240 LNRIVGFKPR PTNVDVPAAV ANLTENIIPL HCKGKRPEDD NNLLDIQYYG MGGYPGFPLN 250 260 270 280 290 300 YYPYYGRLLQ PNYLQPLIAV QFTNITLDTE VRIECRAYGE NLLLSEKDRF QGRFDIKIEM KSS

References

[1]

"Primary sequence and functional expression of a novel ouabain-resistant Na,K-ATPase. The beta subunit modulates potassium activation of the Na,K-pump." Jaisser F., Canessa C.M., Horisberger J.-D., Rossier B.C. J. Biol. Chem. 267:16895-16903(1992) [PubMed: 1380956] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Urinary bladder urothelium.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z11797 mRNA. Translation: CAA77841.1.
PIR	B43451.
3D structure databases
ProteinModelPortal	P30715.
SMR	P30715. Positions 29-74.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG050603.
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. IPR015565. ATPase_P-typ_Na/K-dep_b1su. [Graphical view]
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit. PTHR11523:SF10. Na/K_ATPaseBeta1. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. PS00391. ATPASE_NA_K_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AT1B1_BUFMA

Accession

Primary (citable) accession number: P30715

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	August 10, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program