P30713 (GSTT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase theta-2
EC=2.5.1.18

Alternative name(s):
GST 12-12
GST class-theta-2
Glutathione S-transferase 12
Glutathione S-transferase Yrs-Yrs

Gene names

Name:

Gstt2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the inactivation of reactive sulfate esters in carcinogenic arylmethanols. Highest activity towards ethacrynic acid and cumene hydroperoxide.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm. Nucleus Ref.6.
Tissue specificity	Highest values found in liver followed by testis, adrenal gland, kidney, lung, brain and skeletal muscle. In liver, highest expression found in central vein limiting plate hepatocytes. In lung, expressed mainly in Clara cells of the bronchiolar epithelium and, at low levels, in type II alveolar cells. Ref.6
Sequence similarities	Belongs to the GST superfamily. Theta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glutathione metabolic process Inferred from direct assay PubMed 12038961. Source: MGI
Cellular_component	cytosol Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from direct assay PubMed 12038961. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4 Ref.5
	Chain	2 – 244	243	Glutathione S-transferase theta-2		PRO_0000185943
Regions
	Domain	2 – 82	81	GST N-terminal
	Domain	88 – 230	143	GST C-terminal
	Region	53 – 54	2	Glutathione binding By similarity
	Region	66 – 67	2	Glutathione binding By similarity
Sites
	Binding site	40	1	Glutathione By similarity
Experimental info
	Sequence conflict	14	1	S → C AA sequence Ref.5
	Sequence conflict	36 – 37	2	LK → RC AA sequence Ref.5
	Sequence conflict	42	1	S → C AA sequence Ref.5
	Sequence conflict	44	1	Missing AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P30713 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: B8FCC8B15F003679	FASTA	244	27,439
	10 20 30 40 50 60 MGLELYLDLL SQPSRAVYIF AKKNGIPFQL RTVDLLKGQH LSEQFSQVNC LKKVPVLKDG 70 80 90 100 110 120 SFVLTESTAI LIYLSSKYQV ADHWYPADLQ ARAQVHEYLG WHADNIRGTF GVLLWTKVLG 130 140 150 160 170 180 PLIGVQVPEE KVERNRNSMV LALQRLEDKF LRDRAFIAGQ QVTLADLMSL EELIQPVALG 190 200 210 220 230 240 CNLFEGRPQL TAWRERVEAF LGAELCQEAH NPIMSVLGQA AKKTLPVPPP EAHASMMLRI ARIP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and amino acid sequencing of rat liver class theta glutathione S-transferase Yrs-Yrs inactivating reactive sulfate esters of carcinogenic arylmethanols." Ogura K., Nishiyama T., Okada T., Kajita J., Narihata H., Watabe T., Hiratsuka A., Watabe T. Biochem. Biophys. Res. Commun. 181:1294-1300(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Isolation and characterization of the gene encoding rat class theta glutathione S-transferase subunit yrs." Ogura K., Nishiyama T., Hiratsuka A., Watabe T., Watabe T. Biochem. Biophys. Res. Commun. 205:1250-1256(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[4]	"A new class of rat glutathione S-transferase Yrs-Yrs inactivating reactive sulfate esters as metabolites of carcinogenic arylmethanols." Hiratsuka A., Sebata N., Kawashima K., Okuda H., Ogura K., Watabe T., Satoh K., Hatayama I., Tsuchida S., Ishikawa T., Sato K. J. Biol. Chem. 265:11973-11981(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-26, CHARACTERIZATION. Strain: Sprague-Dawley. Tissue: Liver.
[5]	"Theta, a new class of glutathione transferases purified from rat and man." Meyer D.J., Coles B., Pemble S.E., Gilmore K.S., Fraser G.M., Ketterer B. Biochem. J. 274:409-414(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-47; 140-162; 222-234 AND 238-244. Tissue: Liver.
[6]	"The distribution of theta-class glutathione S-transferases in the liver and lung of mouse, rat and human." Mainwaring G.W., Williams S.M., Foster J.R., Tugwood J., Green T. Biochem. J. 318:297-303(1996) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Tissue: Liver and Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10026 mRNA. Translation: BAA00916.1. D38556 Genomic DNA. Translation: BAA07559.1. BC061856 mRNA. Translation: AAH61856.1.
IPI	IPI00231858.
PIR	JC2425. S14346.
RefSeq	NP_036928.1. NM_012796.2.
UniGene	Rn.87212.
3D structure databases
ProteinModelPortal	P30713.
SMR	P30713. Positions 1-244.
ModBase	Search...
PTM databases
PhosphoSite	P30713.
Proteomic databases
PaxDb	P30713.
PRIDE	P30713.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000037656; ENSRNOP00000033158; ENSRNOG00000028188.
GeneID	29487.
KEGG	rno:29487.
UCSC	RGD:69362. rat.
Organism-specific databases
CTD	2953.
RGD	69362. Gstt2.
Phylogenomic databases
eggNOG	COG0625.
GeneTree	ENSGT00540000069741.
HOGENOM	HOG000125747.
HOVERGEN	HBG051854.
InParanoid	P30713.
KO	K00799.
OMA	ESTAILI.
OrthoDB	EOG43N7F8.
Gene expression databases
ArrayExpress	P30713.
Genevestigator	P30713.
GermOnline	ENSRNOG00000028188. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	609358.

Entry information

Entry name

GSTT2_RAT

Accession

Primary (citable) accession number: P30713
Secondary accession number(s): P36971

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 104 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program