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P30713

- GSTT2_RAT

UniProt

P30713 - GSTT2_RAT

Protein

Glutathione S-transferase theta-2

Gene

Gstt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the inactivation of reactive sulfate esters in carcinogenic arylmethanols. Highest activity towards ethacrynic acid and cumene hydroperoxide.

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 401GlutathioneBy similarity

    GO - Molecular functioni

    1. glutathione transferase activity Source: MGI

    GO - Biological processi

    1. glutathione metabolic process Source: MGI

    Keywords - Molecular functioni

    Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase theta-2 (EC:2.5.1.18)
    Alternative name(s):
    GST 12-12
    GST class-theta-2
    Glutathione S-transferase 12
    Glutathione S-transferase Yrs-Yrs
    Gene namesi
    Name:Gstt2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 20

    Organism-specific databases

    RGDi69362. Gstt2.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 244243Glutathione S-transferase theta-2PRO_0000185943Add
    BLAST

    Proteomic databases

    PaxDbiP30713.
    PRIDEiP30713.

    PTM databases

    PhosphoSiteiP30713.

    Expressioni

    Tissue specificityi

    Highest values found in liver followed by testis, adrenal gland, kidney, lung, brain and skeletal muscle. In liver, highest expression found in central vein limiting plate hepatocytes. In lung, expressed mainly in Clara cells of the bronchiolar epithelium and, at low levels, in type II alveolar cells.1 Publication

    Gene expression databases

    GenevestigatoriP30713.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    MINTiMINT-4565859.

    Structurei

    3D structure databases

    ProteinModelPortaliP30713.
    SMRiP30713. Positions 1-244.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 8281GST N-terminalAdd
    BLAST
    Domaini88 – 230143GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni53 – 542Glutathione bindingBy similarity
    Regioni66 – 672Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Theta family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0625.
    GeneTreeiENSGT00540000069741.
    HOGENOMiHOG000125747.
    HOVERGENiHBG051854.
    InParanoidiP30713.
    KOiK00799.
    OMAiGVPLWVQ.
    OrthoDBiEOG7V1FRJ.
    PhylomeDBiP30713.
    TreeFamiTF325759.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30713-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLELYLDLL SQPSRAVYIF AKKNGIPFQL RTVDLLKGQH LSEQFSQVNC    50
    LKKVPVLKDG SFVLTESTAI LIYLSSKYQV ADHWYPADLQ ARAQVHEYLG 100
    WHADNIRGTF GVLLWTKVLG PLIGVQVPEE KVERNRNSMV LALQRLEDKF 150
    LRDRAFIAGQ QVTLADLMSL EELIQPVALG CNLFEGRPQL TAWRERVEAF 200
    LGAELCQEAH NPIMSVLGQA AKKTLPVPPP EAHASMMLRI ARIP 244
    Length:244
    Mass (Da):27,439
    Last modified:January 23, 2007 - v3
    Checksum:iB8FCC8B15F003679
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141S → C AA sequence (PubMed:1848757)Curated
    Sequence conflicti36 – 372LK → RC AA sequence (PubMed:1848757)Curated
    Sequence conflicti42 – 421S → C AA sequence (PubMed:1848757)Curated
    Sequence conflicti44 – 441Missing AA sequence (PubMed:1848757)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10026 mRNA. Translation: BAA00916.1.
    D38556 Genomic DNA. Translation: BAA07559.1.
    BC061856 mRNA. Translation: AAH61856.1.
    PIRiJC2425.
    S14346.
    RefSeqiNP_036928.1. NM_012796.2.
    UniGeneiRn.87212.

    Genome annotation databases

    EnsembliENSRNOT00000037656; ENSRNOP00000033158; ENSRNOG00000028188.
    GeneIDi29487.
    KEGGirno:29487.
    UCSCiRGD:69362. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10026 mRNA. Translation: BAA00916.1 .
    D38556 Genomic DNA. Translation: BAA07559.1 .
    BC061856 mRNA. Translation: AAH61856.1 .
    PIRi JC2425.
    S14346.
    RefSeqi NP_036928.1. NM_012796.2.
    UniGenei Rn.87212.

    3D structure databases

    ProteinModelPortali P30713.
    SMRi P30713. Positions 1-244.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4565859.

    PTM databases

    PhosphoSitei P30713.

    Proteomic databases

    PaxDbi P30713.
    PRIDEi P30713.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000037656 ; ENSRNOP00000033158 ; ENSRNOG00000028188 .
    GeneIDi 29487.
    KEGGi rno:29487.
    UCSCi RGD:69362. rat.

    Organism-specific databases

    CTDi 2953.
    RGDi 69362. Gstt2.

    Phylogenomic databases

    eggNOGi COG0625.
    GeneTreei ENSGT00540000069741.
    HOGENOMi HOG000125747.
    HOVERGENi HBG051854.
    InParanoidi P30713.
    KOi K00799.
    OMAi GVPLWVQ.
    OrthoDBi EOG7V1FRJ.
    PhylomeDBi P30713.
    TreeFami TF325759.

    Miscellaneous databases

    NextBioi 609358.
    PROi P30713.

    Gene expression databases

    Genevestigatori P30713.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and amino acid sequencing of rat liver class theta glutathione S-transferase Yrs-Yrs inactivating reactive sulfate esters of carcinogenic arylmethanols."
      Ogura K., Nishiyama T., Okada T., Kajita J., Narihata H., Watabe T., Hiratsuka A., Watabe T.
      Biochem. Biophys. Res. Commun. 181:1294-1300(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "Isolation and characterization of the gene encoding rat class theta glutathione S-transferase subunit yrs."
      Ogura K., Nishiyama T., Hiratsuka A., Watabe T., Watabe T.
      Biochem. Biophys. Res. Commun. 205:1250-1256(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    4. "A new class of rat glutathione S-transferase Yrs-Yrs inactivating reactive sulfate esters as metabolites of carcinogenic arylmethanols."
      Hiratsuka A., Sebata N., Kawashima K., Okuda H., Ogura K., Watabe T., Satoh K., Hatayama I., Tsuchida S., Ishikawa T., Sato K.
      J. Biol. Chem. 265:11973-11981(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-26, CHARACTERIZATION.
      Strain: Sprague-Dawley.
      Tissue: Liver.
    5. "Theta, a new class of glutathione transferases purified from rat and man."
      Meyer D.J., Coles B., Pemble S.E., Gilmore K.S., Fraser G.M., Ketterer B.
      Biochem. J. 274:409-414(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-47; 140-162; 222-234 AND 238-244.
      Tissue: Liver.
    6. "The distribution of theta-class glutathione S-transferases in the liver and lung of mouse, rat and human."
      Mainwaring G.W., Williams S.M., Foster J.R., Tugwood J., Green T.
      Biochem. J. 318:297-303(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Tissue: Liver and Lung.

    Entry informationi

    Entry nameiGSTT2_RAT
    AccessioniPrimary (citable) accession number: P30713
    Secondary accession number(s): P36971
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 113 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3