Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P30713

- GSTT2_RAT

UniProt

P30713 - GSTT2_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glutathione S-transferase theta-2

Gene

Gstt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the inactivation of reactive sulfate esters in carcinogenic arylmethanols. Highest activity towards ethacrynic acid and cumene hydroperoxide.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401GlutathioneBy similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: MGI

GO - Biological processi

  1. glutathione metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase theta-2 (EC:2.5.1.18)
Alternative name(s):
GST 12-12
GST class-theta-2
Glutathione S-transferase 12
Glutathione S-transferase Yrs-Yrs
Gene namesi
Name:Gstt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 20

Organism-specific databases

RGDi69362. Gstt2.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 244243Glutathione S-transferase theta-2PRO_0000185943Add
BLAST

Proteomic databases

PaxDbiP30713.
PRIDEiP30713.

PTM databases

PhosphoSiteiP30713.

Expressioni

Tissue specificityi

Highest values found in liver followed by testis, adrenal gland, kidney, lung, brain and skeletal muscle. In liver, highest expression found in central vein limiting plate hepatocytes. In lung, expressed mainly in Clara cells of the bronchiolar epithelium and, at low levels, in type II alveolar cells.1 Publication

Gene expression databases

ExpressionAtlasiP30713. baseline and differential.
GenevestigatoriP30713.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

MINTiMINT-4565859.

Structurei

3D structure databases

ProteinModelPortaliP30713.
SMRiP30713. Positions 1-244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8281GST N-terminalAdd
BLAST
Domaini88 – 230143GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 542Glutathione bindingBy similarity
Regioni66 – 672Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Theta family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0625.
GeneTreeiENSGT00540000069741.
HOGENOMiHOG000125747.
HOVERGENiHBG051854.
InParanoidiP30713.
KOiK00799.
OMAiGVPLWVQ.
OrthoDBiEOG7V1FRJ.
PhylomeDBiP30713.
TreeFamiTF325759.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30713-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLELYLDLL SQPSRAVYIF AKKNGIPFQL RTVDLLKGQH LSEQFSQVNC
60 70 80 90 100
LKKVPVLKDG SFVLTESTAI LIYLSSKYQV ADHWYPADLQ ARAQVHEYLG
110 120 130 140 150
WHADNIRGTF GVLLWTKVLG PLIGVQVPEE KVERNRNSMV LALQRLEDKF
160 170 180 190 200
LRDRAFIAGQ QVTLADLMSL EELIQPVALG CNLFEGRPQL TAWRERVEAF
210 220 230 240
LGAELCQEAH NPIMSVLGQA AKKTLPVPPP EAHASMMLRI ARIP
Length:244
Mass (Da):27,439
Last modified:January 23, 2007 - v3
Checksum:iB8FCC8B15F003679
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141S → C AA sequence (PubMed:1848757)Curated
Sequence conflicti36 – 372LK → RC AA sequence (PubMed:1848757)Curated
Sequence conflicti42 – 421S → C AA sequence (PubMed:1848757)Curated
Sequence conflicti44 – 441Missing AA sequence (PubMed:1848757)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10026 mRNA. Translation: BAA00916.1.
D38556 Genomic DNA. Translation: BAA07559.1.
BC061856 mRNA. Translation: AAH61856.1.
PIRiJC2425.
S14346.
RefSeqiNP_036928.1. NM_012796.2.
UniGeneiRn.87212.

Genome annotation databases

EnsembliENSRNOT00000037656; ENSRNOP00000033158; ENSRNOG00000028188.
GeneIDi29487.
KEGGirno:29487.
UCSCiRGD:69362. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10026 mRNA. Translation: BAA00916.1 .
D38556 Genomic DNA. Translation: BAA07559.1 .
BC061856 mRNA. Translation: AAH61856.1 .
PIRi JC2425.
S14346.
RefSeqi NP_036928.1. NM_012796.2.
UniGenei Rn.87212.

3D structure databases

ProteinModelPortali P30713.
SMRi P30713. Positions 1-244.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4565859.

PTM databases

PhosphoSitei P30713.

Proteomic databases

PaxDbi P30713.
PRIDEi P30713.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000037656 ; ENSRNOP00000033158 ; ENSRNOG00000028188 .
GeneIDi 29487.
KEGGi rno:29487.
UCSCi RGD:69362. rat.

Organism-specific databases

CTDi 2953.
RGDi 69362. Gstt2.

Phylogenomic databases

eggNOGi COG0625.
GeneTreei ENSGT00540000069741.
HOGENOMi HOG000125747.
HOVERGENi HBG051854.
InParanoidi P30713.
KOi K00799.
OMAi GVPLWVQ.
OrthoDBi EOG7V1FRJ.
PhylomeDBi P30713.
TreeFami TF325759.

Miscellaneous databases

NextBioi 609358.
PROi P30713.

Gene expression databases

ExpressionAtlasi P30713. baseline and differential.
Genevestigatori P30713.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and amino acid sequencing of rat liver class theta glutathione S-transferase Yrs-Yrs inactivating reactive sulfate esters of carcinogenic arylmethanols."
    Ogura K., Nishiyama T., Okada T., Kajita J., Narihata H., Watabe T., Hiratsuka A., Watabe T.
    Biochem. Biophys. Res. Commun. 181:1294-1300(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Isolation and characterization of the gene encoding rat class theta glutathione S-transferase subunit yrs."
    Ogura K., Nishiyama T., Hiratsuka A., Watabe T., Watabe T.
    Biochem. Biophys. Res. Commun. 205:1250-1256(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  4. "A new class of rat glutathione S-transferase Yrs-Yrs inactivating reactive sulfate esters as metabolites of carcinogenic arylmethanols."
    Hiratsuka A., Sebata N., Kawashima K., Okuda H., Ogura K., Watabe T., Satoh K., Hatayama I., Tsuchida S., Ishikawa T., Sato K.
    J. Biol. Chem. 265:11973-11981(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-26, CHARACTERIZATION.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  5. "Theta, a new class of glutathione transferases purified from rat and man."
    Meyer D.J., Coles B., Pemble S.E., Gilmore K.S., Fraser G.M., Ketterer B.
    Biochem. J. 274:409-414(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-47; 140-162; 222-234 AND 238-244.
    Tissue: Liver.
  6. "The distribution of theta-class glutathione S-transferases in the liver and lung of mouse, rat and human."
    Mainwaring G.W., Williams S.M., Foster J.R., Tugwood J., Green T.
    Biochem. J. 318:297-303(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Liver and Lung.

Entry informationi

Entry nameiGSTT2_RAT
AccessioniPrimary (citable) accession number: P30713
Secondary accession number(s): P36971
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3