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Protein

Glutathione S-transferase theta-2

Gene

Gstt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the inactivation of reactive sulfate esters in carcinogenic arylmethanols. Highest activity towards ethacrynic acid and cumene hydroperoxide.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40GlutathioneBy similarity1

GO - Molecular functioni

  • glutathione peroxidase activity Source: RGD
  • glutathione transferase activity Source: MGI

GO - Biological processi

  • glutathione metabolic process Source: MGI
  • response to salicylic acid Source: RGD

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

ReactomeiR-RNO-156590. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase theta-2 (EC:2.5.1.18)
Alternative name(s):
GST 12-12
GST class-theta-2
Glutathione S-transferase 12
Glutathione S-transferase Yrs-Yrs
Gene namesi
Name:Gstt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi69362. Gstt2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
  • nucleus Source: RGD

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001859432 – 244Glutathione S-transferase theta-2Add BLAST243

Proteomic databases

PaxDbiP30713.
PRIDEiP30713.

PTM databases

iPTMnetiP30713.
PhosphoSitePlusiP30713.

Expressioni

Tissue specificityi

Highest values found in liver followed by testis, adrenal gland, kidney, lung, brain and skeletal muscle. In liver, highest expression found in central vein limiting plate hepatocytes. In lung, expressed mainly in Clara cells of the bronchiolar epithelium and, at low levels, in type II alveolar cells.1 Publication

Gene expression databases

BgeeiENSRNOG00000028188.
ExpressionAtlasiP30713. differential.
GenevisibleiP30713. RN.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

MINTiMINT-4565859.
STRINGi10116.ENSRNOP00000033158.

Structurei

3D structure databases

ProteinModelPortaliP30713.
SMRiP30713.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 82GST N-terminalAdd BLAST81
Domaini88 – 230GST C-terminalAdd BLAST143

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 54Glutathione bindingBy similarity2
Regioni66 – 67Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Theta family.Curated

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
GeneTreeiENSGT00540000069741.
HOGENOMiHOG000125747.
HOVERGENiHBG051854.
InParanoidiP30713.
KOiK00799.
OMAiVEEYLSW.
OrthoDBiEOG091G0PCG.
PhylomeDBiP30713.
TreeFamiTF325759.

Family and domain databases

InterProiView protein in InterPro
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
PfamiView protein in Pfam
PF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30713-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLELYLDLL SQPSRAVYIF AKKNGIPFQL RTVDLLKGQH LSEQFSQVNC
60 70 80 90 100
LKKVPVLKDG SFVLTESTAI LIYLSSKYQV ADHWYPADLQ ARAQVHEYLG
110 120 130 140 150
WHADNIRGTF GVLLWTKVLG PLIGVQVPEE KVERNRNSMV LALQRLEDKF
160 170 180 190 200
LRDRAFIAGQ QVTLADLMSL EELIQPVALG CNLFEGRPQL TAWRERVEAF
210 220 230 240
LGAELCQEAH NPIMSVLGQA AKKTLPVPPP EAHASMMLRI ARIP
Length:244
Mass (Da):27,439
Last modified:January 23, 2007 - v3
Checksum:iB8FCC8B15F003679
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14S → C AA sequence (PubMed:1848757).Curated1
Sequence conflicti36 – 37LK → RC AA sequence (PubMed:1848757).Curated2
Sequence conflicti42S → C AA sequence (PubMed:1848757).Curated1
Sequence conflicti44Missing AA sequence (PubMed:1848757).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10026 mRNA. Translation: BAA00916.1.
D38556 Genomic DNA. Translation: BAA07559.1.
BC061856 mRNA. Translation: AAH61856.1.
PIRiJC2425.
S14346.
RefSeqiNP_036928.1. NM_012796.2.
UniGeneiRn.87212.

Genome annotation databases

EnsembliENSRNOT00000080203; ENSRNOP00000074436; ENSRNOG00000052415.
ENSRNOT00000081140; ENSRNOP00000071112; ENSRNOG00000052415.
GeneIDi29487.
KEGGirno:29487.
UCSCiRGD:69362. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGSTT2_RAT
AccessioniPrimary (citable) accession number: P30713
Secondary accession number(s): P36971
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families