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Protein

Glutathione S-transferase theta-2

Gene

Gstt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the inactivation of reactive sulfate esters in carcinogenic arylmethanols. Highest activity towards ethacrynic acid and cumene hydroperoxide.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401GlutathioneBy similarity

GO - Molecular functioni

  • glutathione peroxidase activity Source: RGD
  • glutathione transferase activity Source: MGI

GO - Biological processi

  • glutathione metabolic process Source: MGI
  • oxidation-reduction process Source: GOC
  • response to salicylic acid Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiREACT_316307. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase theta-2 (EC:2.5.1.18)
Alternative name(s):
GST 12-12
GST class-theta-2
Glutathione S-transferase 12
Glutathione S-transferase Yrs-Yrs
Gene namesi
Name:Gstt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unassembled WGS sequence

Organism-specific databases

RGDi69362. Gstt2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

  • cytosol Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 244243Glutathione S-transferase theta-2PRO_0000185943Add
BLAST

Proteomic databases

PaxDbiP30713.
PRIDEiP30713.

PTM databases

PhosphoSiteiP30713.

Expressioni

Tissue specificityi

Highest values found in liver followed by testis, adrenal gland, kidney, lung, brain and skeletal muscle. In liver, highest expression found in central vein limiting plate hepatocytes. In lung, expressed mainly in Clara cells of the bronchiolar epithelium and, at low levels, in type II alveolar cells.1 Publication

Gene expression databases

ExpressionAtlasiP30713. baseline and differential.
GenevisibleiP30713. RN.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

MINTiMINT-4565859.
STRINGi10116.ENSRNOP00000033158.

Structurei

3D structure databases

ProteinModelPortaliP30713.
SMRiP30713. Positions 1-244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8281GST N-terminalAdd
BLAST
Domaini88 – 230143GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 542Glutathione bindingBy similarity
Regioni66 – 672Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Theta family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0625.
GeneTreeiENSGT00540000069741.
HOGENOMiHOG000125747.
HOVERGENiHBG051854.
InParanoidiP30713.
KOiK00799.
OMAiGVPLWVQ.
OrthoDBiEOG7V1FRJ.
PhylomeDBiP30713.
TreeFamiTF325759.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30713-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLELYLDLL SQPSRAVYIF AKKNGIPFQL RTVDLLKGQH LSEQFSQVNC
60 70 80 90 100
LKKVPVLKDG SFVLTESTAI LIYLSSKYQV ADHWYPADLQ ARAQVHEYLG
110 120 130 140 150
WHADNIRGTF GVLLWTKVLG PLIGVQVPEE KVERNRNSMV LALQRLEDKF
160 170 180 190 200
LRDRAFIAGQ QVTLADLMSL EELIQPVALG CNLFEGRPQL TAWRERVEAF
210 220 230 240
LGAELCQEAH NPIMSVLGQA AKKTLPVPPP EAHASMMLRI ARIP
Length:244
Mass (Da):27,439
Last modified:January 23, 2007 - v3
Checksum:iB8FCC8B15F003679
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141S → C AA sequence (PubMed:1848757).Curated
Sequence conflicti36 – 372LK → RC AA sequence (PubMed:1848757).Curated
Sequence conflicti42 – 421S → C AA sequence (PubMed:1848757).Curated
Sequence conflicti44 – 441Missing AA sequence (PubMed:1848757).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10026 mRNA. Translation: BAA00916.1.
D38556 Genomic DNA. Translation: BAA07559.1.
BC061856 mRNA. Translation: AAH61856.1.
PIRiJC2425.
S14346.
RefSeqiNP_036928.1. NM_012796.2.
UniGeneiRn.87212.

Genome annotation databases

EnsembliENSRNOT00000080203; ENSRNOP00000074436; ENSRNOG00000052415.
ENSRNOT00000081140; ENSRNOP00000071112; ENSRNOG00000052415.
GeneIDi29487.
KEGGirno:29487.
UCSCiRGD:69362. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10026 mRNA. Translation: BAA00916.1.
D38556 Genomic DNA. Translation: BAA07559.1.
BC061856 mRNA. Translation: AAH61856.1.
PIRiJC2425.
S14346.
RefSeqiNP_036928.1. NM_012796.2.
UniGeneiRn.87212.

3D structure databases

ProteinModelPortaliP30713.
SMRiP30713. Positions 1-244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4565859.
STRINGi10116.ENSRNOP00000033158.

PTM databases

PhosphoSiteiP30713.

Proteomic databases

PaxDbiP30713.
PRIDEiP30713.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000080203; ENSRNOP00000074436; ENSRNOG00000052415.
ENSRNOT00000081140; ENSRNOP00000071112; ENSRNOG00000052415.
GeneIDi29487.
KEGGirno:29487.
UCSCiRGD:69362. rat.

Organism-specific databases

CTDi2953.
RGDi69362. Gstt2.

Phylogenomic databases

eggNOGiCOG0625.
GeneTreeiENSGT00540000069741.
HOGENOMiHOG000125747.
HOVERGENiHBG051854.
InParanoidiP30713.
KOiK00799.
OMAiGVPLWVQ.
OrthoDBiEOG7V1FRJ.
PhylomeDBiP30713.
TreeFamiTF325759.

Enzyme and pathway databases

ReactomeiREACT_316307. Glutathione conjugation.

Miscellaneous databases

NextBioi609358.
PROiP30713.

Gene expression databases

ExpressionAtlasiP30713. baseline and differential.
GenevisibleiP30713. RN.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and amino acid sequencing of rat liver class theta glutathione S-transferase Yrs-Yrs inactivating reactive sulfate esters of carcinogenic arylmethanols."
    Ogura K., Nishiyama T., Okada T., Kajita J., Narihata H., Watabe T., Hiratsuka A., Watabe T.
    Biochem. Biophys. Res. Commun. 181:1294-1300(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Isolation and characterization of the gene encoding rat class theta glutathione S-transferase subunit yrs."
    Ogura K., Nishiyama T., Hiratsuka A., Watabe T., Watabe T.
    Biochem. Biophys. Res. Commun. 205:1250-1256(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  4. "A new class of rat glutathione S-transferase Yrs-Yrs inactivating reactive sulfate esters as metabolites of carcinogenic arylmethanols."
    Hiratsuka A., Sebata N., Kawashima K., Okuda H., Ogura K., Watabe T., Satoh K., Hatayama I., Tsuchida S., Ishikawa T., Sato K.
    J. Biol. Chem. 265:11973-11981(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-26, CHARACTERIZATION.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  5. "Theta, a new class of glutathione transferases purified from rat and man."
    Meyer D.J., Coles B., Pemble S.E., Gilmore K.S., Fraser G.M., Ketterer B.
    Biochem. J. 274:409-414(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-47; 140-162; 222-234 AND 238-244.
    Tissue: Liver.
  6. "The distribution of theta-class glutathione S-transferases in the liver and lung of mouse, rat and human."
    Mainwaring G.W., Williams S.M., Foster J.R., Tugwood J., Green T.
    Biochem. J. 318:297-303(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Liver and Lung.

Entry informationi

Entry nameiGSTT2_RAT
AccessioniPrimary (citable) accession number: P30713
Secondary accession number(s): P36971
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.