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Protein

Glutathione S-transferase theta-1

Gene

GSTT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40Glutathione1 Publication1

GO - Molecular functioni

  • glutathione peroxidase activity Source: UniProtKB
  • glutathione transferase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciZFISH:G66-30906-MONOMER.
BRENDAi2.5.1.18. 2681.
ReactomeiR-HSA-156590. Glutathione conjugation.
SABIO-RKP30711.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase theta-1 (EC:2.5.1.18)
Alternative name(s):
GST class-theta-1
Glutathione transferase T1-1
Gene namesi
Name:GSTT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:4641. GSTT1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176H → Q: Increases activity towards alkylhalogenides, but not hydroperoxides. 1 Publication1
Mutagenesisi234W → R: Facilitates binding of substrates and increases catalytic activity. 1 Publication1

Organism-specific databases

DisGeNETi2952.
OpenTargetsiENSG00000277656.
PharmGKBiPA183.

Chemistry databases

ChEMBLiCHEMBL2141.
DrugBankiDB00958. Carboplatin.
DB00515. Cisplatin.
DB00773. Etoposide.
DB00143. Glutathione.
DB00526. Oxaliplatin.

Polymorphism and mutation databases

BioMutaiGSTT1.
DMDMi21264427.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001859382 – 240Glutathione S-transferase theta-1Add BLAST239

Proteomic databases

EPDiP30711.
MaxQBiP30711.
PaxDbiP30711.
PeptideAtlasiP30711.
PRIDEiP30711.
TopDownProteomicsiP30711-1. [P30711-1]

PTM databases

iPTMnetiP30711.
PhosphoSitePlusiP30711.

Expressioni

Tissue specificityi

Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles.1 Publication

Gene expression databases

BgeeiENSG00000184674.
CleanExiHS_GSTT1.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi109207. 16 interactors.
IntActiP30711. 1 interactor.
STRINGi9606.ENSP00000248935.

Structurei

Secondary structure

1240
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi12 – 23Combined sources12
Beta strandi29 – 32Combined sources4
Helixi35 – 37Combined sources3
Helixi39 – 41Combined sources3
Helixi43 – 48Combined sources6
Beta strandi56 – 59Combined sources4
Beta strandi62 – 65Combined sources4
Helixi67 – 77Combined sources11
Helixi82 – 84Combined sources3
Helixi89 – 101Combined sources13
Helixi102 – 104Combined sources3
Helixi106 – 117Combined sources12
Helixi118 – 123Combined sources6
Helixi130 – 150Combined sources21
Turni151 – 154Combined sources4
Beta strandi155 – 162Combined sources8
Helixi165 – 179Combined sources15
Helixi189 – 202Combined sources14
Helixi204 – 210Combined sources7
Helixi212 – 215Combined sources4
Helixi216 – 219Combined sources4
Helixi225 – 239Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C3NX-ray1.50A/B/C/D2-240[»]
2C3QX-ray1.85A/B/C/D2-240[»]
2C3TX-ray2.40A/B/C/D2-240[»]
ProteinModelPortaliP30711.
SMRiP30711.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30711.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 82GST N-terminalAdd BLAST81
Domaini88 – 220GST C-terminalAdd BLAST133

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 54Glutathione binding1 Publication2
Regioni66 – 67Glutathione binding1 Publication2

Sequence similaritiesi

Belongs to the GST superfamily. Theta family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125747.
HOVERGENiHBG051854.
InParanoidiP30711.
KOiK00799.
OMAiTVKQKLM.
OrthoDBiEOG091G0PCG.
PhylomeDBiP30711.
TreeFamiTF325759.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P30711-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLELYLDLL SQPCRAVYIF AKKNDIPFEL RIVDLIKGQH LSDAFAQVNP
60 70 80 90 100
LKKVPALKDG DFTLTESVAI LLYLTRKYKV PDYWYPQDLQ ARARVDEYLA
110 120 130 140 150
WQHTTLRRSC LRALWHKVMF PVFLGEPVSP QTLAATLAEL DVTLQLLEDK
160 170 180 190 200
FLQNKAFLTG PHISLADLVA ITELMHPVGA GCQVFEGRPK LATWRQRVEA
210 220 230 240
AVGEDLFQEA HEVILKAKDF PPADPTIKQK LMPWVLAMIR
Length:240
Mass (Da):27,335
Last modified:January 23, 2007 - v4
Checksum:iBD19F2BFDEF9F619
GO
Isoform 2 (identifier: P30711-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-118: Missing.

Note: No experimental confirmation available.
Show »
Length:122
Mass (Da):13,517
Checksum:i13F6A21E9D214D56
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43 – 44DA → SD AA sequence (PubMed:1848757).Curated2
Sequence conflicti45F → C in AAH07065 (PubMed:15489334).Curated1
Sequence conflicti126E → G in CAA55935 (PubMed:8198545).Curated1

Polymorphismi

The GSTT1 gene is absent from 38% of the population. The presence or absence of the GSTT1 gene is coincident with the conjugator (GSST1+) and non-conjugator (GSTT1-) phenotypes respectively. The GSTT1+ phenotype can catalyze the glutathione conjugation of dichloromethane.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01450121A → T.Corresponds to variant rs2266635dbSNPEnsembl.1
Natural variantiVAR_014502141D → N.Corresponds to variant rs2266633dbSNPEnsembl.1
Natural variantiVAR_014503169V → I.1 PublicationCorresponds to variant rs2266637dbSNPEnsembl.1
Natural variantiVAR_014504173E → K.Corresponds to variant rs2234953dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0564741 – 118Missing in isoform 2. 1 PublicationAdd BLAST118

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79389 mRNA. Translation: CAA55935.1.
AF435971 mRNA. Translation: AAL31549.1.
AF240786 Genomic DNA. Translation: AAG02374.1.
AB057594 Genomic DNA. Translation: BAB39498.1.
CR456499 mRNA. Translation: CAG30385.1.
BT019951 mRNA. Translation: AAV38754.1.
AP000351 Genomic DNA. No translation available.
Z84718 Genomic DNA. No translation available.
BC007065 mRNA. Translation: AAH07065.1.
PIRiS44358.
RefSeqiNP_000844.2. NM_000853.3. [P30711-1]
NP_001280736.1. NM_001293807.1.
NP_001280737.1. NM_001293808.1. [P30711-2]
NP_001280738.1. NM_001293809.1. [P30711-2]
NP_001280739.1. NM_001293810.1. [P30711-2]
NP_001280740.1. NM_001293811.1. [P30711-2]
NP_001280741.1. NM_001293812.1. [P30711-2]
UniGeneiHs.268573.

Genome annotation databases

EnsembliENST00000612885; ENSP00000481741; ENSG00000277656. [P30711-1]
GeneIDi2952.
KEGGihsa:2952.
UCSCiuc002zze.4. human. [P30711-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

SHMPD

The Singapore human mutation and polymorphism database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79389 mRNA. Translation: CAA55935.1.
AF435971 mRNA. Translation: AAL31549.1.
AF240786 Genomic DNA. Translation: AAG02374.1.
AB057594 Genomic DNA. Translation: BAB39498.1.
CR456499 mRNA. Translation: CAG30385.1.
BT019951 mRNA. Translation: AAV38754.1.
AP000351 Genomic DNA. No translation available.
Z84718 Genomic DNA. No translation available.
BC007065 mRNA. Translation: AAH07065.1.
PIRiS44358.
RefSeqiNP_000844.2. NM_000853.3. [P30711-1]
NP_001280736.1. NM_001293807.1.
NP_001280737.1. NM_001293808.1. [P30711-2]
NP_001280738.1. NM_001293809.1. [P30711-2]
NP_001280739.1. NM_001293810.1. [P30711-2]
NP_001280740.1. NM_001293811.1. [P30711-2]
NP_001280741.1. NM_001293812.1. [P30711-2]
UniGeneiHs.268573.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C3NX-ray1.50A/B/C/D2-240[»]
2C3QX-ray1.85A/B/C/D2-240[»]
2C3TX-ray2.40A/B/C/D2-240[»]
ProteinModelPortaliP30711.
SMRiP30711.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109207. 16 interactors.
IntActiP30711. 1 interactor.
STRINGi9606.ENSP00000248935.

Chemistry databases

ChEMBLiCHEMBL2141.
DrugBankiDB00958. Carboplatin.
DB00515. Cisplatin.
DB00773. Etoposide.
DB00143. Glutathione.
DB00526. Oxaliplatin.

PTM databases

iPTMnetiP30711.
PhosphoSitePlusiP30711.

Polymorphism and mutation databases

BioMutaiGSTT1.
DMDMi21264427.

Proteomic databases

EPDiP30711.
MaxQBiP30711.
PaxDbiP30711.
PeptideAtlasiP30711.
PRIDEiP30711.
TopDownProteomicsiP30711-1. [P30711-1]

Protocols and materials databases

DNASUi2952.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000612885; ENSP00000481741; ENSG00000277656. [P30711-1]
GeneIDi2952.
KEGGihsa:2952.
UCSCiuc002zze.4. human. [P30711-1]

Organism-specific databases

CTDi2952.
DisGeNETi2952.
GeneCardsiGSTT1.
H-InvDBHIX0019638.
HGNCiHGNC:4641. GSTT1.
MIMi600436. gene.
neXtProtiNX_P30711.
OpenTargetsiENSG00000277656.
PharmGKBiPA183.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125747.
HOVERGENiHBG051854.
InParanoidiP30711.
KOiK00799.
OMAiTVKQKLM.
OrthoDBiEOG091G0PCG.
PhylomeDBiP30711.
TreeFamiTF325759.

Enzyme and pathway databases

BioCyciZFISH:G66-30906-MONOMER.
BRENDAi2.5.1.18. 2681.
ReactomeiR-HSA-156590. Glutathione conjugation.
SABIO-RKP30711.

Miscellaneous databases

ChiTaRSiGSTT1. human.
EvolutionaryTraceiP30711.
GeneWikiiGSTT1.
GenomeRNAii2952.
PROiP30711.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000184674.
CleanExiHS_GSTT1.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTT1_HUMAN
AccessioniPrimary (citable) accession number: P30711
Secondary accession number(s): O00226
, Q5TZY2, Q6IC69, Q969K8, Q96IY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 180 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.