ID   ITRA_MOMCH              Reviewed;          28 AA.
AC   P30709;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   08-NOV-2023, entry version 110.
DE   RecName: Full=Trypsin inhibitor A;
OS   Momordica charantia (Bitter gourd) (Balsam pear).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Momordiceae; Momordica.
OX   NCBI_TaxID=3673;
RN   [1]
RP   PROTEIN SEQUENCE, AND X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
RX   PubMed=1551419; DOI=10.1016/0014-5793(92)80346-i;
RA   Huang Q., Liu S., Tang Y., Zeng F., Qian R.;
RT   "Amino acid sequencing of a trypsin inhibitor by refined 1.6 A X-ray
RT   crystal structure of its complex with porcine beta-trypsin.";
RL   FEBS Lett. 297:143-146(1992).
CC   -!- FUNCTION: Inhibits trypsin.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I7 (squash-type serine
CC       protease inhibitor) family. {ECO:0000305}.
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DR   PIR; S20393; S20393.
DR   PDB; 1MCT; X-ray; 1.60 A; I=1-28.
DR   PDBsum; 1MCT; -.
DR   AlphaFoldDB; P30709; -.
DR   SMR; P30709; -.
DR   MINT; P30709; -.
DR   MEROPS; I07.018; -.
DR   EvolutionaryTrace; P30709; -.
DR   Proteomes; UP000504603; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   Gene3D; 4.10.75.20; -; 1.
DR   InterPro; IPR000737; Prot_inh_squash.
DR   InterPro; IPR011052; Proteinase_amylase_inhib_sf.
DR   Pfam; PF00299; Squash; 1.
DR   PRINTS; PR00293; SQUASHINHBTR.
DR   SMART; SM00286; PTI; 1.
DR   SUPFAM; SSF57027; Plant inhibitors of proteinases and amylases; 1.
DR   PROSITE; PS00286; SQUASH_INHIBITOR; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Knottin;
KW   Protease inhibitor; Reference proteome; Secreted;
KW   Serine protease inhibitor.
FT   PEPTIDE         1..28
FT                   /note="Trypsin inhibitor A"
FT                   /id="PRO_0000044389"
FT   SITE            5..6
FT                   /note="Reactive bond"
FT   DISULFID        3..20
FT   DISULFID        10..22
FT   DISULFID        16..27
FT   HELIX           13..15
FT                   /evidence="ECO:0007829|PDB:1MCT"
SQ   SEQUENCE   28 AA;  3133 MW;  67E8E6A2D404FEDF CRC64;
     RSCPRIWMEC TRDSDCMAKC ICVAGHCG
//