P30708 (GPX4_NICSY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Probable phospholipid hydroperoxide glutathione peroxidase Short name=PHGPx EC=1.11.1.12 Alternative name(s): 6P229
Organism	Nicotiana sylvestris (Wood tobacco) (South American tobacco)
Taxonomic identifier	4096 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione By similarity.
Catalytic activity	2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H2O.
Subcellular location	Cytoplasm Potential.
Tissue specificity	Germinating seed, apex, flower, as well as in stressed tissues.
Sequence similarities	Belongs to the glutathione peroxidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	response to oxidative stress Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione peroxidase activity Inferred from electronic annotation. Source: InterPro phospholipid-hydroperoxide glutathione peroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Probable phospholipid hydroperoxide glutathione peroxidase		PRO_0000066633
Sites
	Active site	43	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30708 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 401919069E1E97C7	FASTA	169	18,767
	10 20 30 40 50 60 MASQSSKPQS IYDFTVKDAK GNDVDLSIYK GKVLIIVNVA SQCGLTNSNY TDLTEIYKKY 70 80 90 100 110 120 KDQGLEILAF PCNQFGGQEP GSIEEIQNMV CTRFKAEYPI FDKVDVNGDN AAPLYKFLKS 130 140 150 160 SKGGFFGDSI KWNFSKFLVD KEGNVVDRYS PTTTPASMEK DIKKLLGVA

References

[1]	"Isolation and characterization of a plant cDNA showing homology to animal glutathione peroxidases." Criqui M.-C., Jamet E., Parmentier Y., Marbach J., Durr A., Fleck J. Plant Mol. Biol. 18:623-627(1992) [PubMed: 1536938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Protoplast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60219 mRNA. Translation: CAA42780.1.
PIR	S20501.
3D structure databases
ProteinModelPortal	P30708.
SMR	P30708. Positions 5-168.
ModBase	Search...
Protein family/group databases
PeroxiBase	2868. NsGPx06.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000889. Glutathione_peroxidase. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPX4_NICSY

Accession

Primary (citable) accession number: P30708

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	July 27, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program