P30706 (PLSB_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase, chloroplastic
Short name=GPAT
EC=2.3.1.15

Gene names

Name:

GPAT

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate. The enzyme from chilling-resistant plants discriminates against non-fluid palmitic acid and selects oleic acid whereas the enzyme from sensitive plants accepts both fatty acids. This is an oleate-selective acyltransferase.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Plastid › chloroplast stroma.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Acyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 88	88	Chloroplast Potential
	Chain	89 – 457	369	Glycerol-3-phosphate acyltransferase, chloroplastic		PRO_0000024698
Regions
	Motif	227 – 232	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	P30706 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: C50F52102E4C4EB0	FASTA	457	50,760
	10 20 30 40 50 60 MTDSFAHCAS HINYRHKMKT MFIFSTPCCS PSTAFFSPFR ASNSKPLRST LSLRSSISSS 70 80 90 100 110 120 SITSTSHCSL AFNIVKHKEK NVVSANMTSS VSSRTFLNAQ NEQDVLSGIK KEVEAGTLPA 130 140 150 160 170 180 SIAAGMEEVY LNYKSAVIKS GDPKANEIVL SNMTALLDRI FLDVKEPFVF EAHHKAKREP 190 200 210 220 230 240 FDYYMFGQNY IRPLVDFETS YVGNMPLFIQ MEEQLKQGHN IILMSNHQSE ADPAIIALLL 250 260 270 280 290 300 EMRLPHIAEN LIYVAGDRVI TVPLCKPFSI GRNLICVYSK KHMLDNPELV DMKRKANTRS 310 320 330 340 350 360 RKEMAMLLRS GSQIIWIAPS GGRDRPVANS GEWAPAPFDS SSVDNMRRLV DHSGPPGHIY 370 380 390 400 410 420 PLAILCHDIM PPPLKVEKEI GEKRIISYHG TGISTAPEIS FSNTTAACEN PEKAKDAYTK 430 440 450 ALYDSVTEQY DVLKSAIHGK KGLQASTPVV SLSQPWK

References

[1]	"Purification and cDNA sequencing of an oleate-selective acyl-ACP:sn-glycerol-3-phosphate acyltransferase from pea chloroplasts." Weber S., Wolter F.-P., Buck F., Frentzen M., Heinz E. Plant Mol. Biol. 17:1067-1076(1991) [PubMed: 1932680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 112-134; 310-323 AND 326-345. Strain: cv. Little Marvel. Tissue: Seedling.
[2]	"Specificities and selectivities of glycerol-3-phosphate acyltransferase and monoacylglycerol-3-phosphate acyltransferase from pea and spinach chloroplasts." Frentzen M., Heinz E., McKeon T.A., Stumpf P.K. Eur. J. Biochem. 129:629-636(1983) [PubMed: 6825679] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59041 mRNA. Translation: CAA41769.1.
PIR	S18239.
3D structure databases
ProteinModelPortal	P30706.
SMR	P30706. Positions 93-456.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR016222. G3P_O-acylTrfase_chlp. IPR023083. G3P_O-acylTrfase_N. [Graphical view]
Gene3D	G3DSA:1.10.1200.50. G3P_O-acylTrfase_N. 1 hit.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000431. Glycerol-3-P_O-acyltransfrase. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_PEA

Accession

Primary (citable) accession number: P30706

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 31, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program