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P30685 (1B35_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HLA class I histocompatibility antigen, B-35 alpha chain
Alternative name(s):
MHC class I antigen B*35
Gene names
Name:HLA-B
Synonyms:HLAB
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the presentation of foreign antigens to the immune system.

Subunit structure

Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Post-translational modification

Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.

Polymorphism

The following alleles of B-35 are known: B*35:01, B*35:02, B*35:03, B*35:04, B*35:05 (B35-G), B*35:06 (B35-K), B*35:07, B*35:08, B*35:25, B*35:28, B*35:29 (B*KG), B*35:30, B*35:32 (B*TMUL) and B*35:36. The sequence shown is that of B*35:01.

Sequence similarities

Belongs to the MHC class I family.

Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Biological processHost-virus interaction
Immunity
   Cellular componentMembrane
MHC I
   Coding sequence diversityPolymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent

Traceable author statement. Source: Reactome

antigen processing and presentation of peptide antigen via MHC class I

Traceable author statement. Source: Reactome

cytokine-mediated signaling pathway

Traceable author statement. Source: Reactome

interferon-gamma-mediated signaling pathway

Traceable author statement. Source: Reactome

positive regulation of T cell mediated cytotoxicity

Inferred from electronic annotation. Source: InterPro

regulation of immune response

Traceable author statement. Source: Reactome

type I interferon signaling pathway

Traceable author statement. Source: Reactome

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentER to Golgi transport vesicle membrane

Traceable author statement. Source: Reactome

Golgi apparatus

Inferred from sequence or structural similarity. Source: UniProt

Golgi membrane

Traceable author statement. Source: Reactome

MHC class I protein complex

Inferred from sequence or structural similarity. Source: UniProt

cell surface

Inferred from sequence or structural similarity. Source: UniProt

early endosome membrane

Traceable author statement. Source: Reactome

endoplasmic reticulum

Inferred from sequence or structural similarity. Source: UniProt

integral component of lumenal side of endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

phagocytic vesicle membrane

Traceable author statement. Source: Reactome

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionpeptide antigen binding

Inferred from sequence or structural similarity. Source: UniProt

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424
Chain25 – 362338HLA class I histocompatibility antigen, B-35 alpha chain
PRO_0000018840

Regions

Topological domain25 – 308284Extracellular Potential
Transmembrane309 – 33224Helical; Potential
Topological domain333 – 36230Cytoplasmic Potential
Domain209 – 29587Ig-like C1-type
Region25 – 11490Alpha-1
Region115 – 20692Alpha-2
Region207 – 29892Alpha-3
Region299 – 30810Connecting peptide

Amino acid modifications

Glycosylation1101N-linked (GlcNAc...) By similarity
Disulfide bond125 ↔ 188
Disulfide bond227 ↔ 283

Natural variations

Natural variant401G → V in allele B*35:07.
VAR_016393
Natural variant481A → S in allele B*35:25.
VAR_016394
Natural variant691T → E in allele B*35:25; requires 2 nucleotide substitutions.
VAR_016395
Natural variant871N → E in allele B*35:28; requires 2 nucleotide substitutions.
VAR_016396
Natural variant911F → S in allele B*35:28.
VAR_016397
Natural variant981Y → D in allele B*35:29.
VAR_016398
Natural variant1071G → D in allele B*35:36.
VAR_016399
Natural variant118 – 1192II → TL in allele B*35:05 and allele B*35:32.
VAR_016400
Natural variant1211R → S in allele B*35:05 and allele B*35:30.
VAR_016401
Natural variant1271L → V in allele B*35:32.
VAR_016402
Natural variant1331L → F in allele B*35:02.
VAR_016403
Natural variant1381D → N in allele B*35:02, allele B*35:04 and allele B*35:06.
VAR_016404
Natural variant1401S → F in allele B*35:06 and allele B*35:36.
VAR_016405
Natural variant1401S → Y in allele B*35:02, allele B*35:03 and allele B*35:04. Ref.13
VAR_016406
Natural variant1801L → R in allele B*35:08.
VAR_016407

Secondary structure

...................................................... 362
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P30685 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 52906854FC43E7A6

FASTA36240,455
        10         20         30         40         50         60 
MRVTAPRTVL LLLWGAVALT ETWAGSHSMR YFYTAMSRPG RGEPRFIAVG YVDDTQFVRF 

        70         80         90        100        110        120 
DSDAASPRTE PRAPWIEQEG PEYWDRNTQI FKTNTQTYRE SLRNLRGYYN QSEAGSHIIQ 

       130        140        150        160        170        180 
RMYGCDLGPD GRLLRGHDQS AYDGKDYIAL NEDLSSWTAA DTAAQITQRK WEAARVAEQL 

       190        200        210        220        230        240 
RAYLEGLCVE WLRRYLENGK ETLQRADPPK THVTHHPVSD HEATLRCWAL GFYPAEITLT 

       250        260        270        280        290        300 
WQRDGEDQTQ DTELVETRPA GDRTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP 

       310        320        330        340        350        360 
SSQSTIPIVG IVAGLAVLAV VVIGAVVATV MCRRKSSGGK GGSYSQAASS DSAQGSDVSL 


TA 

« Hide

References

« Hide 'large scale' references
[1]"The structure of HLA-B35 suggests that it is derived from HLA-Bw58 by two genetic mechanisms."
Ooba T., Hayashi H., Karaki S., Tanabe M., Kano K., Takiguchi M.
Immunogenetics 30:76-80(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE B*35:01).
[2]"Complete nucleotide sequence of a genomic clone encoding HLA-B35 isolated from a Caucasian individual of Hispanic origin. Identification of a new variant of HLA-B35."
Chertkoff L.P., Herrera M., Fainboim L., Satz M.L.
Hum. Immunol. 31:153-158(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE B*35:02).
[3]"The HLA-A,B 'negative' mutant cell line C1R expresses a novel HLA-B35 allele, which also has a point mutation in the translation initiation codon."
Zemmour J., Little A.-M., Schendel D.J., Parham P.
J. Immunol. 148:1941-1948(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE B*35:03).
[4]"Polymorphism of human minor histocompatibility antigens: T cell recognition of human minor histocompatibility peptides presented by HLA-B35 subtype molecules."
Beck Y., Satz L., Takamiya Y., Nakayama S., Ling L., Ishikawa Y., Nagao T., Uchida H., Tokunaga K., Mueller C., Juji T., Takiguchi M.
J. Exp. Med. 181:2037-2048(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*35:03).
Tissue: Blood.
[5]"Unusual HLA-B alleles in two tribes of Brazilian Indians."
Belich M.P., Madrigal J.A., Hildebrand W.H., Zemmour J., Williams R.C., Luz R., Petzl-Erler M.L., Parham P.
Nature 357:326-329(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELES B*35:05 AND B*35:06).
[6]"Isolation and characterization of two new functional subtypes of HLA-B35."
Theiler G., Pando M., Delfino J.M., Takiguchi M., Satz M.L.
Tissue Antigens 41:143-147(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES B*35:07 AND B*35:08).
[7]"Microheterogeneity in HLA-B35 alleles influences peptide-dependent allorecognition by cytotoxic T cells but not binding of a peptide-restricted monoclonal antibody."
Steinle A., Reinhardt C., Noessner E., Uchanska-Ziegler B., Ziegler A., Schendel D.J.
Hum. Immunol. 38:261-269(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE B*35:08).
[8]"New recombinant HLA-B alleles in a tribe of South American Amerindians indicate rapid evolution of MHC class I loci."
Watkins D.I., McAdam S.N., Liu X., Stang C.R., Milford E.L., Levine C.G., Garber T.L., Dogon A.L., Lord C.I., Ghim S.H., Troup G.M., Hughes A.L., Letvin N.L.
Nature 357:329-333(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 9-362 (ALLELE B*35:04).
[9]"Routine HLA-B genotyping with PCR-sequence-specific oligonucleotides (PCR-SSO) detects eight new alleles: B*0807, B*0809, B*1551, B*3529, B*3532, B*4025, B*5304 and B*5508."
Kennedy C.T., Dodd R., Le T., Wallace R., Ng G., Greville W.D., Kennedy A., Taverniti A., Moses J.H., Clow N., Watson N., Dunckley H.
Tissue Antigens 55:266-270(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-206 (ALLELES B*35:29 AND B*35:32).
[10]"Twenty-nine new HLA-B alleles associated with antigens in the 5C CREG."
Steiner N.K., Kosman C., Jones P.F., Gans C.P., Rodriguez-Marino S.G., Rizzuto G., Baldassarre L.A., Edson S., Koester R., Sese D., Mitton W., Ng J., Hartzman R.J., Hurley C.K.
Tissue Antigens 57:481-485(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELES B*35:25; B*35:28; B*35:29; B*35:30 AND B*35:36).
[11]"An altered position of the alpha 2 helix of MHC class I is revealed by the crystal structure of HLA-B*3501."
Smith K.J., Reid S.W., Stuart D.I., McMichael A.J., Jones E.Y., Bell J.I.
Immunity 4:203-214(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-300.
[12]"Decamer-like conformation of a nona-peptide bound to HLA-B*3501 due to non-standard positioning of the C-terminus."
Menssen R., Orth P., Ziegler A., Saenger W.
J. Mol. Biol. 285:645-653(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] TYR-140, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M28115 expand/collapse EMBL AC list , M28109, M28110, M28111, M28112, M28113, M28114 Genomic DNA. Translation: AAA59617.1.
M63454 Genomic DNA. Translation: AAA59682.1.
M81798 mRNA. Translation: AAA59684.1.
D50299 mRNA. Translation: BAA08828.1.
M84385 mRNA. Translation: AAA59635.1.
M84381 mRNA. Translation: AAA59631.1.
L04695 mRNA. Translation: AAA59694.1.
L04696 mRNA. Translation: AAA52674.1.
Z22651 mRNA. Translation: CAA80366.1.
M86403 mRNA. No translation available.
AH007565 Genomic DNA. Translation: AAD23460.1.
AH007706 Genomic DNA. Translation: AAD30277.1.
AH006372 Genomic DNA. Translation: AAC32570.1.
AH007604 Genomic DNA. Translation: AAD27538.1.
AH008126 Genomic DNA. Translation: AAD51745.1.
AH007584 Genomic DNA. Translation: AAD26151.1.
AH009759 Genomic DNA. Translation: AAG01819.1.
PIRA45880.
I54298.
I56133.
I61904.
I61907.
I81233.
UniGeneHs.654404.
Hs.77961.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1A1NX-ray2.00A25-300[»]
1A9BX-ray3.20A/D25-301[»]
1A9EX-ray2.50A25-301[»]
1CG9X-ray2.70A25-301[»]
1XH3X-ray1.48A25-300[»]
1ZHKX-ray1.60A25-300[»]
1ZHLX-ray1.50A25-300[»]
1ZSDX-ray1.70A25-300[»]
2AK4X-ray2.50A/F/K/Q25-300[»]
2AXFX-ray1.80A25-300[»]
2AXGX-ray2.00A25-300[»]
2CIKX-ray1.75A25-300[»]
2FYYX-ray1.50A25-300[»]
2FZ3X-ray1.90A25-300[»]
2H6PX-ray1.90A25-300[»]
2NW3X-ray1.70A25-300[»]
2NX5X-ray2.70A/F/K/Q25-300[»]
3BW9X-ray1.75A25-300[»]
3BWAX-ray1.30A25-300[»]
3KWWX-ray2.18A25-300[»]
3KXFX-ray3.10A/C/I/K25-300[»]
3LKNX-ray2.00A25-300[»]
3LKOX-ray1.80A25-300[»]
3LKPX-ray1.80A25-300[»]
3LKQX-ray1.80A25-300[»]
3LKRX-ray2.00A25-300[»]
3LKSX-ray1.90A25-300[»]
3MV7X-ray2.00A25-300[»]
3MV8X-ray2.10A25-300[»]
3MV9X-ray2.70A25-300[»]
3VFSX-ray1.85A25-300[»]
3VFTX-ray1.95A25-300[»]
3VFUX-ray1.65A25-300[»]
3VFVX-ray1.55A25-300[»]
3VFWX-ray2.30A25-300[»]
4PR5X-ray1.80A25-300[»]
4PRNX-ray1.65A25-300[»]
ProteinModelPortalP30685.
SMRP30685. Positions 25-300.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP30685. 1 interaction.
MINTMINT-1516663.

Polymorphism databases

DMDM231390.

Proteomic databases

PRIDEP30685.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000359635; ENSP00000352656; ENSG00000206450.

Organism-specific databases

GeneCardsGC06M031321.
GC06Mn31311.
H-InvDBHIX0167180.
HGNCHGNC:4932. HLA-B.
MIM142830. gene.
neXtProtNX_P30685.
GenAtlasSearch...

Phylogenomic databases

HOVERGENHBG016709.
InParanoidP30685.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

CleanExHS_HLA-B.
GenevestigatorP30685.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]
PfamPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]
PRINTSPR01638. MHCCLASSI.
SMARTSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMSSF54452. SSF54452. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHLA-B. human.
EvolutionaryTraceP30685.
SOURCESearch...

Entry information

Entry name1B35_HUMAN
AccessionPrimary (citable) accession number: P30685
Secondary accession number(s): O62919 expand/collapse secondary AC list , P30468, P30469, P30470, P30471, P30472, P30473, P30474, Q9GJM7, Q9TPV2, Q9TQH3, Q9TQH9, Q9TQN4, Q9TQN6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 9, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM