Reviewed, UniProtKB/Swiss-Prot P30685 (1B35_HUMAN)

Names and origin

Protein names

Recommended name:
    HLA class I histocompatibility antigen, B-35 alpha chain
Alternative name(s):
    MHC class I antigen B*35

Gene names

Name:	HLA-B
Synonyms:	HLAB

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the presentation of foreign antigens to the immune system.
Subunit structure	Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.
Polymorphism	The following alleles of B-35 are known: B*3501, B*3502, B*3503, B*3504, B*3505 (B35-G), B*3506 (B35-K), B*3507, B*3508, B*3525, B*3528, B*3529 (B*KG), B*3530, B*3532 (B*TMUL) and B*3536. The sequence shown is that of B*3501.
Sequence similarities	Belongs to the MHC class I family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Immune response
Cellular component	MHC I Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane
PTM	Disulfide bond Glycoprotein Ubl conjugation
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	antigen processing and presentation of peptide antigen via MHC class I Inferred from electronic annotation. Source: UniProtKB-KW immune response Inferred from electronic annotation. Source: UniProtKB-KW interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	MHC class I protein complex Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Chain

25 – 362

338

HLA class I histocompatibility antigen, B-35 alpha chain

PRO_0000018840

Regions

Topological domain

25 – 308

284

Extracellular Potential

Transmembrane

309 – 332

24

Potential

Topological domain

333 – 362

30

Cytoplasmic Potential

Domain

209 – 295

87

Ig-like C1-type

Region

25 – 114

90

Alpha-1

Region

115 – 206

92

Alpha-2

Region

207 – 298

92

Alpha-3

Region

299 – 308

10

Connecting peptide

Amino acid modifications

Glycosylation

110

1

N-linked (GlcNAc...) By similarity

Disulfide bond

125 ↔ 188

Disulfide bond

227 ↔ 283

Natural variations

Natural variant

40

1

G → V in allele B*3507.

VAR_016393

Natural variant

48

1

A → S in allele B*3525.

VAR_016394

Natural variant

69

1

T → E in allele B*3525; requires 2 nucleotide substitutions.

VAR_016395

Natural variant

87

1

N → E in allele B*3528; requires 2 nucleotide substitutions.

VAR_016396

Natural variant

91

1

F → S in allele B*3528.

VAR_016397

Natural variant

98

1

Y → D in allele B*3529.

VAR_016398

Natural variant

107

1

G → D in allele B*3536.

VAR_016399

Natural variant

118 – 119

2

II → TL in allele B*3505 and allele B*3532.

VAR_016400

Natural variant

121

1

R → S in allele B*3505 and allele B*3530.

VAR_016401

Natural variant

127

1

L → V in allele B*3532.

VAR_016402

Natural variant

133

1

L → F in allele B*3502.

VAR_016403

Natural variant

138

1

D → N in allele B*3502, allele B*3504 and allele B*3506.

VAR_016404

Natural variant

140

1

S → F in allele B*3506 and allele B*3536.

VAR_016405

Natural variant

140

1

S → Y in allele B*3502, allele B*3503 and allele B*3504.

VAR_016406

Natural variant

180

1

L → R in allele B*3508.

VAR_016407

Secondary structure

1

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362

Helix Strand Turn

Details...

Beta strand

27 – 36

10

Beta strand

41 – 43

3

Beta strand

45 – 52

8

Beta strand

55 – 61

7

Beta strand

64 – 66

3

Helix

74 – 76

3

Helix

81 – 108

28

Beta strand

118 – 127

10

Beta strand

133 – 142

10

Beta strand

145 – 150

6

Beta strand

157 – 161

5

Helix

162 – 173

12

Helix

176 – 185

10

Helix

187 – 198

12

Helix

200 – 203

4

Beta strand

210 – 217

8

Beta strand

219 – 235

17

Beta strand

238 – 243

6

Helix

249 – 251

3

Beta strand

261 – 263

3

Beta strand

265 – 274

10

Helix

278 – 280

3

Beta strand

281 – 286

6

Beta strand

294 – 296

3

Sequences

	Sequence		Length	Mass (Da)
	P30685-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 52906854FC43E7A6	FASTA	362	40,455
	10 20 30 40 50 60 MRVTAPRTVL LLLWGAVALT ETWAGSHSMR YFYTAMSRPG RGEPRFIAVG YVDDTQFVRF 70 80 90 100 110 120 DSDAASPRTE PRAPWIEQEG PEYWDRNTQI FKTNTQTYRE SLRNLRGYYN QSEAGSHIIQ 130 140 150 160 170 180 RMYGCDLGPD GRLLRGHDQS AYDGKDYIAL NEDLSSWTAA DTAAQITQRK WEAARVAEQL 190 200 210 220 230 240 RAYLEGLCVE WLRRYLENGK ETLQRADPPK THVTHHPVSD HEATLRCWAL GFYPAEITLT 250 260 270 280 290 300 WQRDGEDQTQ DTELVETRPA GDRTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP 310 320 330 340 350 360 SSQSTIPIVG IVAGLAVLAV VVIGAVVATV MCRRKSSGGK GGSYSQAASS DSAQGSDVSL TA

References

[1]	"The structure of HLA-B35 suggests that it is derived from HLA-Bw58 by two genetic mechanisms." Ooba T., Hayashi H., Karaki S., Tanabe M., Kano K., Takiguchi M. Immunogenetics 30:76-80(1989) [PubMed: 2788131] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE B*3501).
[2]	"Complete nucleotide sequence of a genomic clone encoding HLA-B35 isolated from a Caucasian individual of Hispanic origin. Identification of a new variant of HLA-B35." Chertkoff L.P., Herrera M., Fainboim L., Satz M.L. Hum. Immunol. 31:153-158(1991) [PubMed: 1890016] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE B*3502).
[3]	"The HLA-A,B 'negative' mutant cell line C1R expresses a novel HLA-B35 allele, which also has a point mutation in the translation initiation codon." Zemmour J., Little A.-M., Schendel D.J., Parham P. J. Immunol. 148:1941-1948(1992) [PubMed: 1541831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE B*3503).
[4]	"Polymorphism of human minor histocompatibility antigens: T cell recognition of human minor histocompatibility peptides presented by HLA-B35 subtype molecules." Beck Y., Satz L., Takamiya Y., Nakayama S., Ling L., Ishikawa Y., Nagao T., Uchida H., Tokunaga K., Mueller C., Juji T., Takiguchi M. J. Exp. Med. 181:2037-2048(1995) [PubMed: 7759996] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*3503). Tissue: Blood.
[5]	"Unusual HLA-B alleles in two tribes of Brazilian Indians." Belich M.P., Madrigal J.A., Hildebrand W.H., Zemmour J., Williams R.C., Luz R., Petzl-Erler M.L., Parham P. Nature 357:326-329(1992) [PubMed: 1317015] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ALLELES B*3505 AND B*3506).
[6]	"Isolation and characterization of two new functional subtypes of HLA-B35." Theiler G., Pando M., Delfino J.M., Takiguchi M., Satz M.L. Tissue Antigens 41:143-147(1993) [PubMed: 8316945] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES B*3507 AND B*3508).
[7]	"Microheterogeneity in HLA-B35 alleles influences peptide-dependent allorecognition by cytotoxic T cells but not binding of a peptide-restricted monoclonal antibody." Steinle A., Reinhardt C., Noessner E., Uchanska-Ziegler B., Ziegler A., Schendel D.J. Hum. Immunol. 38:261-269(1993) [PubMed: 8138421] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE B*3508).
[8]	"New recombinant HLA-B alleles in a tribe of South American Amerindians indicate rapid evolution of MHC class I loci." Watkins D.I., McAdam S.N., Liu X., Stang C.R., Milford E.L., Levine C.G., Garber T.L., Dogon A.L., Lord C.I., Ghim S.H., Troup G.M., Hughes A.L., Letvin N.L. Nature 357:329-333(1992) [PubMed: 1589035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 9-362 (ALLELE B*3504).
[9]	"Routine HLA-B genotyping with PCR-sequence-specific oligonucleotides (PCR-SSO) detects eight new alleles: B*0807, B*0809, B*1551, B*3529, B*3532, B*4025, B*5304 and B*5508." Kennedy C.T., Dodd R., Le T., Wallace R., Ng G., Greville W.D., Kennedy A., Taverniti A., Moses J.H., Clow N., Watson N., Dunckley H. Tissue Antigens 55:266-270(2000) [PubMed: 10777103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-206 (ALLELES B*3529 AND B*3532).
[10]	"Twenty-nine new HLA-B alleles associated with antigens in the 5C CREG." Steiner N.K., Kosman C., Jones P.F., Gans C.P., Rodriguez-Marino S.G., Rizzuto G., Baldassarre L.A., Edson S., Koester R., Sese D., Mitton W., Ng J., Hartzman R.J., Hurley C.K. Tissue Antigens 57:481-485(2001) [PubMed: 11556976] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELES B*3525; B*3528; B*3529; B*3530 AND B*3536).
[11]	"An altered position of the alpha 2 helix of MHC class I is revealed by the crystal structure of HLA-B*3501." Smith K.J., Reid S.W., Stuart D.I., McMichael A.J., Jones E.Y., Bell J.I. Immunity 4:203-214(1996) [PubMed: 8624811] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-300.
[12]	"Decamer-like conformation of a nona-peptide bound to HLA-B*3501 due to non-standard positioning of the C-terminus." Menssen R., Orth P., Ziegler A., Saenger W. J. Mol. Biol. 285:645-653(1999) [PubMed: 9878435] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M28115 , M28109, M28110, M28111, M28112, M28113, M28114 Genomic DNA. Translation: AAA59617.1.
M63454 Genomic DNA. Translation: AAA59682.1.
M81798 mRNA. Translation: AAA59684.1.
D50299 mRNA. Translation: BAA08828.1.
M84385 mRNA. Translation: AAA59635.1.
M84381 mRNA. Translation: AAA59631.1.
L04695 mRNA. Translation: AAA59694.1.
L04696 mRNA. Translation: AAA52674.1.
Z22651 mRNA. Translation: CAA80366.1.
M86403 mRNA. No translation available.
AF117771, AF117770 Genomic DNA. Translation: AAD23460.1.
AF134867, AF134866 Genomic DNA. Translation: AAD30277.1.
AF061864, AF061863 Genomic DNA. Translation: AAC32570.1.
AF108429, AF108428 Genomic DNA. Translation: AAD27538.1.
AF176078, AF176077 Genomic DNA. Translation: AAD51745.1.
AF110505, AF110504 Genomic DNA. Translation: AAD26151.1.
AF282766, AF282765 Genomic DNA. Translation: AAG01819.1.

IPI

IPI00646083.

PIR

A45880.
I54298.
I56133.
I61904.
I61907.
I81233.

UniGene

Hs.703277
Hs.707171
Hs.77961

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A1N	X-ray	2.00	A	25-300	[»]
1A9B	X-ray	3.20	A/D	25-301	[»]
1A9E	X-ray	2.50	A	25-301	[»]
1CG9	X-ray	2.70	A	25-301	[»]
1XH3	X-ray	1.48	A	25-300	[»]
1ZHK	X-ray	1.60	A	25-300	[»]
1ZHL	X-ray	1.50	A	25-300	[»]
1ZSD	X-ray	1.70	A	25-300	[»]
2AK4	X-ray	2.50	A/F/K/Q	25-300	[»]
2AXF	X-ray	1.80	A	25-300	[»]
2AXG	X-ray	2.00	A	25-300	[»]
2CIK	X-ray	1.75	A	25-300	[»]
2FYY	X-ray	1.50	A	25-300	[»]
2FZ3	X-ray	1.90	A	25-300	[»]
2H6P	X-ray	1.90	A	25-300	[»]
2NW3	X-ray	1.70	A	25-300	[»]
2NX5	X-ray	2.70	A/F/K/Q	25-300	[»]
3BW9	X-ray	1.75	A	25-300	[»]
3BWA	X-ray	1.30	A	25-300	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:6056N.

Proteomic databases

PRIDE

P30685.

Genome annotation databases

Ensembl

ENSG00000206450. Homo sapiens. [Contig view]

Organism-specific databases

GeneCards

GC06M031345.
GC06Mf31371.

H-InvDB

HIX0005715.

HGNC

HGNC:4932. HLA-B.

MIM

142830. gene.

PharmGKB

PA35056.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P30685.

Enzyme and pathway databases

Reactome

REACT_6900. Signaling in Immune system.

Gene expression databases

Bgee

P30685.

CleanEx

HS_HLA-B.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
G3DSA:3.30.500.10. MHC_I-like_Ag-recog. 1 hit.

Pfam

PF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]

PRINTS

PR01638. MHCCLASSI.

ProDom

PD000050. MHC_I. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00407. IGc1. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

1B35_HUMAN

Accession

Primary (citable) accession number: P30685
Secondary accession number(s): O62919 , P30468, P30469, P30470, P30471, P30472, P30473, P30474, Q9GJM7, Q9TPV2, Q9TQH3, Q9TQH9, Q9TQN4, Q9TQN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	July 7, 2009
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)