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P30681

- HMGB2_MOUSE

UniProt

P30681 - HMGB2_MOUSE

Protein

High mobility group protein B2

Gene

Hmgb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS).1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi9 – 7971HMG box 1PROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi95 – 16369HMG box 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chemoattractant activity Source: Ensembl
    2. damaged DNA binding Source: Ensembl
    3. DNA binding, bending Source: UniProtKB
    4. protein domain specific binding Source: MGI
    5. sequence-specific DNA binding transcription factor activity Source: Ensembl
    6. transcription regulatory region DNA binding Source: Ensembl

    GO - Biological processi

    1. base-excision repair, DNA ligation Source: UniProtKB
    2. cell chemotaxis Source: Ensembl
    3. cellular response to lipopolysaccharide Source: Ensembl
    4. male gonad development Source: MGI
    5. negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
    6. negative regulation of transcription, DNA-templated Source: Ensembl
    7. positive regulation of DNA binding Source: MGI
    8. positive regulation of endothelial cell proliferation Source: Ensembl
    9. positive regulation of erythrocyte differentiation Source: Ensembl
    10. positive regulation of megakaryocyte differentiation Source: Ensembl
    11. positive regulation of nuclease activity Source: Ensembl
    12. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    13. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    14. response to steroid hormone Source: MGI
    15. spermatid nucleus differentiation Source: MGI
    16. spermatogenesis Source: MGI

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_205002. Activation of DNA fragmentation factor.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High mobility group protein B2
    Alternative name(s):
    High mobility group protein 2
    Short name:
    HMG-2
    Gene namesi
    Name:Hmgb2
    Synonyms:Hmg2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:96157. Hmgb2.

    Subcellular locationi

    GO - Cellular componenti

    1. condensed chromosome Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. extracellular space Source: Ensembl
    4. nucleus Source: UniProtKB
    5. perinuclear region of cytoplasm Source: UniProtKB
    6. protein complex Source: Ensembl

    Keywords - Cellular componenti

    Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 210209High mobility group protein B2PRO_0000048535Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei30 – 301N6-acetyllysine1 Publication
    Modified residuei114 – 1141N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP30681.
    PaxDbiP30681.
    PRIDEiP30681.

    PTM databases

    PhosphoSiteiP30681.

    Expressioni

    Gene expression databases

    BgeeiP30681.
    CleanExiMM_HMGB2.
    GenevestigatoriP30681.

    Interactioni

    Subunit structurei

    Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Directly interacts with SET.1 Publication

    Protein-protein interaction databases

    BioGridi220633. 3 interactions.
    DIPiDIP-899N.
    IntActiP30681. 3 interactions.
    MINTiMINT-4097539.

    Structurei

    3D structure databases

    ProteinModelPortaliP30681.
    SMRiP30681. Positions 1-166.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi186 – 21025Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the HMGB family.Curated
    Contains 2 HMG box DNA-binding domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5648.
    GeneTreeiENSGT00730000110464.
    HOGENOMiHOG000197861.
    HOVERGENiHBG009000.
    InParanoidiP30681.
    KOiK11295.
    OMAiFCAEHRP.
    OrthoDBiEOG7WHHBQ.
    PhylomeDBiP30681.
    TreeFamiTF105371.

    Family and domain databases

    Gene3Di1.10.30.10. 2 hits.
    InterProiIPR009071. HMG_box_dom.
    IPR017967. HMG_boxA_CS.
    [Graphical view]
    PfamiPF00505. HMG_box. 1 hit.
    PF09011. HMG_box_2. 1 hit.
    [Graphical view]
    SMARTiSM00398. HMG. 2 hits.
    [Graphical view]
    SUPFAMiSSF47095. SSF47095. 2 hits.
    PROSITEiPS00353. HMG_BOX_1. 1 hit.
    PS50118. HMG_BOX_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30681-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK    50
    TMSAKEKSKF EDLAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS 100
    AFFLFCSENR PKIKIEHPGL SIGDTAKKLG EMWSEQSAKD KQPYEQKAAK 150
    LKEKYEKDIA AYRAKGKSEA GKKGPGRPTG SKKKNEPEDE EEEEEEEEEE 200
    DDEEEEEDEE 210
    Length:210
    Mass (Da):24,162
    Last modified:January 23, 2007 - v3
    Checksum:i45D1F667DB4ED94D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71N → I in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti10 – 101R → L in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti33 – 331D → N in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti41 – 411F → I in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti47 – 471E → K in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti57 – 571K → N in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti70 – 723RYD → CYY in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti80 – 801P → S in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti103 – 1031F → C in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti117 – 1171H → Y in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti140 – 1401D → E in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti159 – 1591I → F in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti164 – 1641A → V in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti179 – 1791T → A in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti184 – 19310KNEPEDEEEE → NDSED in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti202 – 2021D → E in CAA47900. (PubMed:1408807)Curated
    Sequence conflicti202 – 2021D → E in AAG36939. 1 PublicationCurated
    Sequence conflicti202 – 2021D → E in AAH02050. (PubMed:15489334)Curated
    Sequence conflicti204 – 2041E → G in CAA47900. (PubMed:1408807)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67668 mRNA. Translation: CAA47900.1.
    Z46757 mRNA. Translation: CAA86727.1.
    AF267733 Genomic DNA. Translation: AAG36939.1.
    AK003773 mRNA. Translation: BAB22988.1.
    AK008443 mRNA. Translation: BAB25672.1.
    AK012568 mRNA. Translation: BAB28323.1.
    AK135296 mRNA. Translation: BAE22481.1.
    AK135297 mRNA. Translation: BAE22482.1.
    AK146212 mRNA. Translation: BAE26982.1.
    BC002050 mRNA. Translation: AAH02050.1.
    BC046759 mRNA. Translation: AAH46759.1.
    BC083108 mRNA. Translation: AAH83108.1.
    CCDSiCCDS40343.1.
    PIRiS26062.
    S54774.
    RefSeqiNP_032278.1. NM_008252.3.
    XP_006509587.1. XM_006509524.1.
    UniGeneiMm.276881.
    Mm.279998.

    Genome annotation databases

    EnsembliENSMUST00000067925; ENSMUSP00000065940; ENSMUSG00000054717.
    GeneIDi97165.
    KEGGimmu:97165.
    UCSCiuc009lsx.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67668 mRNA. Translation: CAA47900.1 .
    Z46757 mRNA. Translation: CAA86727.1 .
    AF267733 Genomic DNA. Translation: AAG36939.1 .
    AK003773 mRNA. Translation: BAB22988.1 .
    AK008443 mRNA. Translation: BAB25672.1 .
    AK012568 mRNA. Translation: BAB28323.1 .
    AK135296 mRNA. Translation: BAE22481.1 .
    AK135297 mRNA. Translation: BAE22482.1 .
    AK146212 mRNA. Translation: BAE26982.1 .
    BC002050 mRNA. Translation: AAH02050.1 .
    BC046759 mRNA. Translation: AAH46759.1 .
    BC083108 mRNA. Translation: AAH83108.1 .
    CCDSi CCDS40343.1.
    PIRi S26062.
    S54774.
    RefSeqi NP_032278.1. NM_008252.3.
    XP_006509587.1. XM_006509524.1.
    UniGenei Mm.276881.
    Mm.279998.

    3D structure databases

    ProteinModelPortali P30681.
    SMRi P30681. Positions 1-166.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 220633. 3 interactions.
    DIPi DIP-899N.
    IntActi P30681. 3 interactions.
    MINTi MINT-4097539.

    PTM databases

    PhosphoSitei P30681.

    Proteomic databases

    MaxQBi P30681.
    PaxDbi P30681.
    PRIDEi P30681.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000067925 ; ENSMUSP00000065940 ; ENSMUSG00000054717 .
    GeneIDi 97165.
    KEGGi mmu:97165.
    UCSCi uc009lsx.2. mouse.

    Organism-specific databases

    CTDi 3148.
    MGIi MGI:96157. Hmgb2.

    Phylogenomic databases

    eggNOGi COG5648.
    GeneTreei ENSGT00730000110464.
    HOGENOMi HOG000197861.
    HOVERGENi HBG009000.
    InParanoidi P30681.
    KOi K11295.
    OMAi FCAEHRP.
    OrthoDBi EOG7WHHBQ.
    PhylomeDBi P30681.
    TreeFami TF105371.

    Enzyme and pathway databases

    Reactomei REACT_205002. Activation of DNA fragmentation factor.

    Miscellaneous databases

    NextBioi 352589.
    PROi P30681.
    SOURCEi Search...

    Gene expression databases

    Bgeei P30681.
    CleanExi MM_HMGB2.
    Genevestigatori P30681.

    Family and domain databases

    Gene3Di 1.10.30.10. 2 hits.
    InterProi IPR009071. HMG_box_dom.
    IPR017967. HMG_boxA_CS.
    [Graphical view ]
    Pfami PF00505. HMG_box. 1 hit.
    PF09011. HMG_box_2. 1 hit.
    [Graphical view ]
    SMARTi SM00398. HMG. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47095. SSF47095. 2 hits.
    PROSITEi PS00353. HMG_BOX_1. 1 hit.
    PS50118. HMG_BOX_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a mouse cDNA encoding the non-histone chromosomal high mobility group protein-2 (HMG-2)."
      Stolzenburg F., Dinkl E., Grummt F.
      Nucleic Acids Res. 20:4927-4927(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: PCC4.
    2. Stolzenburg F.
      Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "High mobility group protein 2 functionally interacts with the POU domains of octamer transcription factors."
      Zwilling S., Koenig H., Wirth T.
      EMBO J. 14:1198-1208(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Partial male infertility in mice lacking chromosomal protein HMG2."
      Ronfani L., Bianchi M.E.
      Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: 129/Sv.
    5. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and DBA/2.
      Tissue: Small intestine and Wolffian duct.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Mammary gland.
    7. "Stimulation of V(D)J cleavage by high mobility group proteins."
      van Gent D.C., Hiom K., Paull T.T., Gellert M.
      EMBO J. 16:2665-2670(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE RAG COMPLEX.
    8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-30 AND LYS-114, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiHMGB2_MOUSE
    AccessioniPrimary (citable) accession number: P30681
    Secondary accession number(s): Q3UXT1, Q9EQD5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 130 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3