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P30681

- HMGB2_MOUSE

UniProt

P30681 - HMGB2_MOUSE

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Protein

High mobility group protein B2

Gene

Hmgb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS).1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi9 – 7971HMG box 1PROSITE-ProRule annotationAdd
BLAST
DNA bindingi95 – 16369HMG box 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chemoattractant activity Source: Ensembl
  2. damaged DNA binding Source: Ensembl
  3. DNA binding, bending Source: UniProtKB
  4. poly(A) RNA binding Source: Ensembl
  5. protein domain specific binding Source: MGI
  6. sequence-specific DNA binding transcription factor activity Source: Ensembl
  7. transcription regulatory region DNA binding Source: Ensembl

GO - Biological processi

  1. base-excision repair, DNA ligation Source: UniProtKB
  2. cell chemotaxis Source: Ensembl
  3. cellular response to lipopolysaccharide Source: Ensembl
  4. male gonad development Source: MGI
  5. negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
  6. negative regulation of transcription, DNA-templated Source: Ensembl
  7. positive regulation of DNA binding Source: MGI
  8. positive regulation of endothelial cell proliferation Source: Ensembl
  9. positive regulation of erythrocyte differentiation Source: Ensembl
  10. positive regulation of megakaryocyte differentiation Source: Ensembl
  11. positive regulation of nuclease activity Source: Ensembl
  12. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  13. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  14. response to steroid hormone Source: MGI
  15. spermatid nucleus differentiation Source: MGI
  16. spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_205002. Activation of DNA fragmentation factor.

Names & Taxonomyi

Protein namesi
Recommended name:
High mobility group protein B2
Alternative name(s):
High mobility group protein 2
Short name:
HMG-2
Gene namesi
Name:Hmgb2
Synonyms:Hmg2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:96157. Hmgb2.

Subcellular locationi

GO - Cellular componenti

  1. condensed chromosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. extracellular space Source: Ensembl
  4. nucleus Source: UniProtKB
  5. perinuclear region of cytoplasm Source: UniProtKB
  6. protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 210209High mobility group protein B2PRO_0000048535Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301N6-acetyllysine1 Publication
Modified residuei114 – 1141N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP30681.
PaxDbiP30681.
PRIDEiP30681.

PTM databases

PhosphoSiteiP30681.

Expressioni

Gene expression databases

BgeeiP30681.
CleanExiMM_HMGB2.
GenevestigatoriP30681.

Interactioni

Subunit structurei

Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Directly interacts with SET.1 Publication

Protein-protein interaction databases

BioGridi220633. 3 interactions.
DIPiDIP-899N.
IntActiP30681. 3 interactions.
MINTiMINT-4097539.

Structurei

3D structure databases

ProteinModelPortaliP30681.
SMRiP30681. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi186 – 21025Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the HMGB family.Curated
Contains 2 HMG box DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5648.
GeneTreeiENSGT00760000119164.
HOGENOMiHOG000197861.
HOVERGENiHBG009000.
InParanoidiP30681.
KOiK11295.
OMAiFCAEHRP.
OrthoDBiEOG7WHHBQ.
PhylomeDBiP30681.
TreeFamiTF105371.

Family and domain databases

Gene3Di1.10.30.10. 2 hits.
InterProiIPR009071. HMG_box_dom.
IPR017967. HMG_boxA_CS.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
PF09011. HMG_box_2. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 2 hits.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 2 hits.
PROSITEiPS00353. HMG_BOX_1. 1 hit.
PS50118. HMG_BOX_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30681-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK
60 70 80 90 100
TMSAKEKSKF EDLAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS
110 120 130 140 150
AFFLFCSENR PKIKIEHPGL SIGDTAKKLG EMWSEQSAKD KQPYEQKAAK
160 170 180 190 200
LKEKYEKDIA AYRAKGKSEA GKKGPGRPTG SKKKNEPEDE EEEEEEEEEE
210
DDEEEEEDEE
Length:210
Mass (Da):24,162
Last modified:January 23, 2007 - v3
Checksum:i45D1F667DB4ED94D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71N → I in CAA47900. (PubMed:1408807)Curated
Sequence conflicti10 – 101R → L in CAA47900. (PubMed:1408807)Curated
Sequence conflicti33 – 331D → N in CAA47900. (PubMed:1408807)Curated
Sequence conflicti41 – 411F → I in CAA47900. (PubMed:1408807)Curated
Sequence conflicti47 – 471E → K in CAA47900. (PubMed:1408807)Curated
Sequence conflicti57 – 571K → N in CAA47900. (PubMed:1408807)Curated
Sequence conflicti70 – 723RYD → CYY in CAA47900. (PubMed:1408807)Curated
Sequence conflicti80 – 801P → S in CAA47900. (PubMed:1408807)Curated
Sequence conflicti103 – 1031F → C in CAA47900. (PubMed:1408807)Curated
Sequence conflicti117 – 1171H → Y in CAA47900. (PubMed:1408807)Curated
Sequence conflicti140 – 1401D → E in CAA47900. (PubMed:1408807)Curated
Sequence conflicti159 – 1591I → F in CAA47900. (PubMed:1408807)Curated
Sequence conflicti164 – 1641A → V in CAA47900. (PubMed:1408807)Curated
Sequence conflicti179 – 1791T → A in CAA47900. (PubMed:1408807)Curated
Sequence conflicti184 – 19310KNEPEDEEEE → NDSED in CAA47900. (PubMed:1408807)Curated
Sequence conflicti202 – 2021D → E in CAA47900. (PubMed:1408807)Curated
Sequence conflicti202 – 2021D → E in AAG36939. 1 PublicationCurated
Sequence conflicti202 – 2021D → E in AAH02050. (PubMed:15489334)Curated
Sequence conflicti204 – 2041E → G in CAA47900. (PubMed:1408807)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67668 mRNA. Translation: CAA47900.1.
Z46757 mRNA. Translation: CAA86727.1.
AF267733 Genomic DNA. Translation: AAG36939.1.
AK003773 mRNA. Translation: BAB22988.1.
AK008443 mRNA. Translation: BAB25672.1.
AK012568 mRNA. Translation: BAB28323.1.
AK135296 mRNA. Translation: BAE22481.1.
AK135297 mRNA. Translation: BAE22482.1.
AK146212 mRNA. Translation: BAE26982.1.
BC002050 mRNA. Translation: AAH02050.1.
BC046759 mRNA. Translation: AAH46759.1.
BC083108 mRNA. Translation: AAH83108.1.
CCDSiCCDS40343.1.
PIRiS26062.
S54774.
RefSeqiNP_032278.1. NM_008252.3.
XP_006509587.1. XM_006509524.1.
UniGeneiMm.276881.
Mm.279998.

Genome annotation databases

EnsembliENSMUST00000067925; ENSMUSP00000065940; ENSMUSG00000054717.
GeneIDi97165.
KEGGimmu:97165.
UCSCiuc009lsx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67668 mRNA. Translation: CAA47900.1 .
Z46757 mRNA. Translation: CAA86727.1 .
AF267733 Genomic DNA. Translation: AAG36939.1 .
AK003773 mRNA. Translation: BAB22988.1 .
AK008443 mRNA. Translation: BAB25672.1 .
AK012568 mRNA. Translation: BAB28323.1 .
AK135296 mRNA. Translation: BAE22481.1 .
AK135297 mRNA. Translation: BAE22482.1 .
AK146212 mRNA. Translation: BAE26982.1 .
BC002050 mRNA. Translation: AAH02050.1 .
BC046759 mRNA. Translation: AAH46759.1 .
BC083108 mRNA. Translation: AAH83108.1 .
CCDSi CCDS40343.1.
PIRi S26062.
S54774.
RefSeqi NP_032278.1. NM_008252.3.
XP_006509587.1. XM_006509524.1.
UniGenei Mm.276881.
Mm.279998.

3D structure databases

ProteinModelPortali P30681.
SMRi P30681. Positions 1-166.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 220633. 3 interactions.
DIPi DIP-899N.
IntActi P30681. 3 interactions.
MINTi MINT-4097539.

PTM databases

PhosphoSitei P30681.

Proteomic databases

MaxQBi P30681.
PaxDbi P30681.
PRIDEi P30681.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000067925 ; ENSMUSP00000065940 ; ENSMUSG00000054717 .
GeneIDi 97165.
KEGGi mmu:97165.
UCSCi uc009lsx.2. mouse.

Organism-specific databases

CTDi 3148.
MGIi MGI:96157. Hmgb2.

Phylogenomic databases

eggNOGi COG5648.
GeneTreei ENSGT00760000119164.
HOGENOMi HOG000197861.
HOVERGENi HBG009000.
InParanoidi P30681.
KOi K11295.
OMAi FCAEHRP.
OrthoDBi EOG7WHHBQ.
PhylomeDBi P30681.
TreeFami TF105371.

Enzyme and pathway databases

Reactomei REACT_205002. Activation of DNA fragmentation factor.

Miscellaneous databases

NextBioi 352589.
PROi P30681.
SOURCEi Search...

Gene expression databases

Bgeei P30681.
CleanExi MM_HMGB2.
Genevestigatori P30681.

Family and domain databases

Gene3Di 1.10.30.10. 2 hits.
InterProi IPR009071. HMG_box_dom.
IPR017967. HMG_boxA_CS.
[Graphical view ]
Pfami PF00505. HMG_box. 1 hit.
PF09011. HMG_box_2. 1 hit.
[Graphical view ]
SMARTi SM00398. HMG. 2 hits.
[Graphical view ]
SUPFAMi SSF47095. SSF47095. 2 hits.
PROSITEi PS00353. HMG_BOX_1. 1 hit.
PS50118. HMG_BOX_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a mouse cDNA encoding the non-histone chromosomal high mobility group protein-2 (HMG-2)."
    Stolzenburg F., Dinkl E., Grummt F.
    Nucleic Acids Res. 20:4927-4927(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: PCC4.
  2. Stolzenburg F.
    Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "High mobility group protein 2 functionally interacts with the POU domains of octamer transcription factors."
    Zwilling S., Koenig H., Wirth T.
    EMBO J. 14:1198-1208(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Partial male infertility in mice lacking chromosomal protein HMG2."
    Ronfani L., Bianchi M.E.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 129/Sv.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and DBA/2.
    Tissue: Small intestine and Wolffian duct.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Mammary gland.
  7. "Stimulation of V(D)J cleavage by high mobility group proteins."
    van Gent D.C., Hiom K., Paull T.T., Gellert M.
    EMBO J. 16:2665-2670(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE RAG COMPLEX.
  8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-30 AND LYS-114, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiHMGB2_MOUSE
AccessioniPrimary (citable) accession number: P30681
Secondary accession number(s): Q3UXT1, Q9EQD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3