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Protein

Somatostatin receptor type 2

Gene

Sstr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for somatostatin-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. Inhibits calcium entry by suppressing voltage-dependent calcium channels. Acts as the functionally dominant somatostatin receptor in pancreatic alpha- and beta-cells where it mediates the inhibitory effect of somatostatin-14 on hormone secretion. Inhibits cell growth through enhancement of MAPK1 and MAPK2 phosphorylation and subsequent up-regulation of CDKN1B. Stimulates neuronal migration and axon outgrowth and may participate in neuron development and maturation during brain development. Mediates negative regulation of insulin receptor signaling through PTPN6. Inactivates SSTR3 receptor function following heterodimerization.4 Publications

GO - Molecular functioni

  • neuropeptide binding Source: GO_Central
  • PDZ domain binding Source: UniProtKB
  • somatostatin receptor activity Source: RGD

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: Ensembl
  • cellular response to estradiol stimulus Source: RGD
  • cellular response to glucocorticoid stimulus Source: RGD
  • cerebellum development Source: RGD
  • chemical synaptic transmission Source: GO_Central
  • forebrain development Source: RGD
  • G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
  • negative regulation of cell proliferation Source: GO_Central
  • peristalsis Source: RGD
  • regulation of muscle contraction Source: RGD
  • response to starvation Source: RGD
  • spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Somatostatin receptor type 2
Short name:
SS-2-R
Short name:
SS2-R
Short name:
SS2R
Alternative name(s):
SRIF-1
Gene namesi
Name:Sstr2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi3763. Sstr2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 43ExtracellularSequence analysisAdd BLAST43
Transmembranei44 – 67Helical; Name=1Sequence analysisAdd BLAST24
Topological domaini68 – 78CytoplasmicSequence analysisAdd BLAST11
Transmembranei79 – 103Helical; Name=2Sequence analysisAdd BLAST25
Topological domaini104 – 118ExtracellularSequence analysisAdd BLAST15
Transmembranei119 – 138Helical; Name=3Sequence analysisAdd BLAST20
Topological domaini139 – 161CytoplasmicSequence analysisAdd BLAST23
Transmembranei162 – 181Helical; Name=4Sequence analysisAdd BLAST20
Topological domaini182 – 207ExtracellularSequence analysisAdd BLAST26
Transmembranei208 – 229Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini230 – 253CytoplasmicSequence analysisAdd BLAST24
Transmembranei254 – 278Helical; Name=6Sequence analysisAdd BLAST25
Topological domaini279 – 288ExtracellularSequence analysis10
Transmembranei289 – 303Helical; Name=7Sequence analysisAdd BLAST15
Topological domaini304 – 369CytoplasmicSequence analysisAdd BLAST66

GO - Cellular componenti

  • cytoplasm Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • neuron projection Source: GO_Central
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2978.
GuidetoPHARMACOLOGYi356.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000701231 – 369Somatostatin receptor type 2Add BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi9N-linked (GlcNAc...)Sequence analysis1
Glycosylationi22N-linked (GlcNAc...)Sequence analysis1
Glycosylationi29N-linked (GlcNAc...)Sequence analysis1
Glycosylationi32N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi115 ↔ 193PROSITE-ProRule annotation
Lipidationi328S-palmitoyl cysteineSequence analysis1
Modified residuei341Phosphoserine1 Publication1
Modified residuei343Phosphoserine1 Publication1
Modified residuei348Phosphoserine1 Publication1
Modified residuei353Phosphothreonine1 Publication1
Modified residuei354Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylated on serine and threonine residues in response to agonist stimulation, leading to receptor desensitization and rapid internalization. Phosphorylated to a greater extent on serine than threonine residues. Threonine phosphorylation is required for arrestin binding and receptor endocytosis but is not necessary for desensitization.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP30680.
PRIDEiP30680.

PTM databases

iPTMnetiP30680.
PhosphoSitePlusiP30680.

Expressioni

Tissue specificityi

Cortex, hippocampus, pituitary gland, colon, kidney, and adrenal gland. In the developing nervous system, expressed from E12 when it is restricted to postmitotic neuronal populations leaving the ventricular zone. From E12 on, expressed in migrating neuronal populations in numerous developing regions including the cerebral cortex, hippocampus and ganglionic eminences. Also detected in the deep part of the external granular layer of the cerebellum, the rostral migratory stream and a subset of axons and neurons. Expressed in the medial forebrain bundle, rostral migratory stream and cerebellum during development but not in adulthood.3 Publications

Gene expression databases

BgeeiENSRNOG00000002793.
GenevisibleiP30680. RN.

Interactioni

Subunit structurei

Homodimer and heterodimer with SSTR3 and SSTR5. Heterodimerization with SSTR3 inactivates SSTR3 receptor function. Heterodimerization with SSTR5 is enhanced by agonist stimulation of SSTR2 and increases SSTR2 cell growth inhibition activity. Following agonist stimulation, homodimers dissociate into monomers which is required for receptor internalization. Interacts with beta-arrestin; this interaction is necessary for receptor internalization and is destabilized by heterodimerization with SSTR5 which results in increased recycling of SSTR2 to the cell surface. Interacts (via C-terminus) with SHANK1 (via PDZ domain).2 Publications

GO - Molecular functioni

  • PDZ domain binding Source: UniProtKB

Protein-protein interaction databases

BioGridi248525. 4 interactors.
IntActiP30680. 1 interactor.
MINTiMINT-151186.
STRINGi10116.ENSRNOP00000003735.

Chemistry databases

BindingDBiP30680.

Structurei

3D structure databases

ProteinModelPortaliP30680.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230485.
HOVERGENiHBG106919.
InParanoidiP30680.
KOiK04218.
OMAiSTVVITC.
OrthoDBiEOG091G0HEN.
PhylomeDBiP30680.
TreeFamiTF315737.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000586. Somatstn_rcpt.
IPR002074. Somatstn_rcpt_2.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00246. SOMATOSTATNR.
PR00588. SOMATOSTTN2R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P30680-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELTSEQFNG SQVWIPSPFD LNGSLGPSNG SNQTEPYYDM TSNAVLTFIY
60 70 80 90 100
FVVCVVGLCG NTLVIYVILR YAKMKTITNI YILNLAIADE LFMLGLPFLA
110 120 130 140 150
MQVALVHWPF GKAICRVVMT VDGINQFTSI FCLTVMSIDR YLAVVHPIKS
160 170 180 190 200
AKWRRPRTAK MINVAVWGVS LLVILPIMIY AGLRSNQWGR SSCTINWPGE
210 220 230 240 250
SGAWYTGFII YAFILGFLVP LTIICLCYLF IIIKVKSSGI RVGSSKRKKS
260 270 280 290 300
EKKVTRMVSI VVAVFIFCWL PFYIFNVSSV SVAISPTPAL KGMFDFVVIL
310 320 330 340 350
TYANSCANPI LYAFLSDNFK KSFQNVLCLV KVSGAEDGER SDSKQDKSRL
360
NETTETQRTL LNGDLQTSI
Length:369
Mass (Da):41,200
Last modified:April 1, 1993 - v1
Checksum:i4990E489E88D7D19
GO
Isoform B (identifier: P30680-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     332-369: VSGAEDGERSDSKQDKSRLNETTETQRTLLNGDLQTSI → ADNSKTGEEDTMAWV

Show »
Length:346
Mass (Da):38,673
Checksum:i2D75370906A660E3
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001924332 – 369VSGAE…LQTSI → ADNSKTGEEDTMAWV in isoform B. CuratedAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96817 mRNA. Translation: AAA42166.1.
M93273 mRNA. Translation: AAA42165.1.
PIRiA45291.
RefSeqiNP_062221.1. NM_019348.1. [P30680-1]
UniGeneiRn.202974.
Rn.9929.

Genome annotation databases

EnsembliENSRNOT00000003735; ENSRNOP00000003735; ENSRNOG00000002793. [P30680-1]
GeneIDi54305.
KEGGirno:54305.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96817 mRNA. Translation: AAA42166.1.
M93273 mRNA. Translation: AAA42165.1.
PIRiA45291.
RefSeqiNP_062221.1. NM_019348.1. [P30680-1]
UniGeneiRn.202974.
Rn.9929.

3D structure databases

ProteinModelPortaliP30680.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248525. 4 interactors.
IntActiP30680. 1 interactor.
MINTiMINT-151186.
STRINGi10116.ENSRNOP00000003735.

Chemistry databases

BindingDBiP30680.
ChEMBLiCHEMBL2978.
GuidetoPHARMACOLOGYi356.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP30680.
PhosphoSitePlusiP30680.

Proteomic databases

PaxDbiP30680.
PRIDEiP30680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003735; ENSRNOP00000003735; ENSRNOG00000002793. [P30680-1]
GeneIDi54305.
KEGGirno:54305.

Organism-specific databases

CTDi6752.
RGDi3763. Sstr2.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230485.
HOVERGENiHBG106919.
InParanoidiP30680.
KOiK04218.
OMAiSTVVITC.
OrthoDBiEOG091G0HEN.
PhylomeDBiP30680.
TreeFamiTF315737.

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiP30680.

Gene expression databases

BgeeiENSRNOG00000002793.
GenevisibleiP30680. RN.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000586. Somatstn_rcpt.
IPR002074. Somatstn_rcpt_2.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00246. SOMATOSTATNR.
PR00588. SOMATOSTTN2R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSR2_RAT
AccessioniPrimary (citable) accession number: P30680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.