P30680 (SSR2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Somatostatin receptor type 2
Short name=SS-2-R
Short name=SS2-R
Short name=SS2R

Alternative name(s):
SRIF-1

Gene names

Name:

Sstr2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels.
Subunit structure	The C-terminus interacts with SHANK1 PDZ domain By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Cortex, hippocampus, pituitary gland, colon adrenals, pancreas-derived cell line and pancreatic tumor.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara cellular response to estradiol stimulus Inferred from expression pattern PubMed 9564833. Source: RGD cellular response to glucocorticoid stimulus Inferred from expression pattern PubMed 14512709. Source: RGD cerebellum development Inferred from expression pattern PubMed 9166718. Source: RGD forebrain development Inferred from expression pattern PubMed 10805921. Source: RGD peristalsis Inferred from mutant phenotype PubMed 12504886. Source: RGD regulation of muscle contraction Inferred from mutant phenotype PubMed 11897621. Source: RGD response to starvation Inferred from expression pattern PubMed 7956902. Source: RGD spermatogenesis Inferred from expression pattern PubMed 9421433. Source: RGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 9322965. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 9322965. Source: RGD
Molecular_function	somatostatin receptor activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P30680-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P30680-2)

The sequence of this isoform differs from the canonical sequence as follows:
     332-369: VSGAEDGERSDSKQDKSRLNETTETQRTLLNGDLQTSI → ADNSKTGEEDTMAWV

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Somatostatin receptor type 2		PRO_0000070123
Regions
	Topological domain	1 – 43	43	Extracellular Potential
	Transmembrane	44 – 67	24	Helical; Name=1; Potential
	Topological domain	68 – 78	11	Cytoplasmic Potential
	Transmembrane	79 – 103	25	Helical; Name=2; Potential
	Topological domain	104 – 118	15	Extracellular Potential
	Transmembrane	119 – 138	20	Helical; Name=3; Potential
	Topological domain	139 – 161	23	Cytoplasmic Potential
	Transmembrane	162 – 181	20	Helical; Name=4; Potential
	Topological domain	182 – 207	26	Extracellular Potential
	Transmembrane	208 – 229	22	Helical; Name=5; Potential
	Topological domain	230 – 253	24	Cytoplasmic Potential
	Transmembrane	254 – 278	25	Helical; Name=6; Potential
	Topological domain	279 – 288	10	Extracellular Potential
	Transmembrane	289 – 303	15	Helical; Name=7; Potential
	Topological domain	304 – 369	66	Cytoplasmic Potential
Amino acid modifications
	Lipidation	328	1	S-palmitoyl cysteine Potential
	Glycosylation	9	1	N-linked (GlcNAc...) Potential
	Glycosylation	22	1	N-linked (GlcNAc...) Potential
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	32	1	N-linked (GlcNAc...) Potential
	Disulfide bond	115 ↔ 193		By similarity
Natural variations
	Alternative sequence	332 – 369	38	VSGAE…LQTSI → ADNSKTGEEDTMAWV in isoform B.		VSP_001924

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 4990E489E88D7D19	FASTA	369	41,200
	10 20 30 40 50 60 MELTSEQFNG SQVWIPSPFD LNGSLGPSNG SNQTEPYYDM TSNAVLTFIY FVVCVVGLCG 70 80 90 100 110 120 NTLVIYVILR YAKMKTITNI YILNLAIADE LFMLGLPFLA MQVALVHWPF GKAICRVVMT 130 140 150 160 170 180 VDGINQFTSI FCLTVMSIDR YLAVVHPIKS AKWRRPRTAK MINVAVWGVS LLVILPIMIY 190 200 210 220 230 240 AGLRSNQWGR SSCTINWPGE SGAWYTGFII YAFILGFLVP LTIICLCYLF IIIKVKSSGI 250 260 270 280 290 300 RVGSSKRKKS EKKVTRMVSI VVAVFIFCWL PFYIFNVSSV SVAISPTPAL KGMFDFVVIL 310 320 330 340 350 360 TYANSCANPI LYAFLSDNFK KSFQNVLCLV KVSGAEDGER SDSKQDKSRL NETTETQRTL LNGDLQTSI
	Isoform B [UniParc]. Checksum: 2D75370906A660E3	FASTA	346	38,673
	10 20 30 40 50 60 MELTSEQFNG SQVWIPSPFD LNGSLGPSNG SNQTEPYYDM TSNAVLTFIY FVVCVVGLCG 70 80 90 100 110 120 NTLVIYVILR YAKMKTITNI YILNLAIADE LFMLGLPFLA MQVALVHWPF GKAICRVVMT 130 140 150 160 170 180 VDGINQFTSI FCLTVMSIDR YLAVVHPIKS AKWRRPRTAK MINVAVWGVS LLVILPIMIY 190 200 210 220 230 240 AGLRSNQWGR SSCTINWPGE SGAWYTGFII YAFILGFLVP LTIICLCYLF IIIKVKSSGI 250 260 270 280 290 300 RVGSSKRKKS EKKVTRMVSI VVAVFIFCWL PFYIFNVSSV SVAISPTPAL KGMFDFVVIL 310 320 330 340 TYANSCANPI LYAFLSDNFK KSFQNVLCLV KADNSKTGEE DTMAWV

References

[1]	"Expression cloning of a rat brain somatostatin receptor cDNA." Kluxen F.-W., Bruns C., Luebbert H. Proc. Natl. Acad. Sci. U.S.A. 89:4618-4622(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Partial amino acid sequence of a somatostatin receptor isolated from GH4C1 pituitary cells." Hulmes J.D., Corbett M., Zysk J.R., Boehlen P., Eppler C.M. Biochem. Biophys. Res. Commun. 184:131-136(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 76-81; 179-189; 294-320 AND 350-358. Tissue: Pituitary.
[3]	"Multiple gene transcripts of the somatostatin receptor SSTR2: tissue selective distribution and cAMP regulation." Patel Y.C., Greenwood M., Kent G., Panetta R., Srikant C.B. Biochem. Biophys. Res. Commun. 192:288-294(1993) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96817 mRNA. Translation: AAA42166.1. M93273 mRNA. Translation: AAA42165.1.
IPI	IPI00211113. IPI00230998.
PIR	A45291.
RefSeq	NP_062221.1. NM_019348.1.
UniGene	Rn.202974. Rn.9929.
3D structure databases
ProteinModelPortal	P30680.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-151186.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P30680.
Proteomic databases
PRIDE	P30680.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000003734; ENSRNOP00000003734; ENSRNOG00000002793. ENSRNOT00000003735; ENSRNOP00000003735; ENSRNOG00000002793.
GeneID	54305.
KEGG	rno:54305.
Organism-specific databases
CTD	6752.
RGD	3763. Sstr2.
Phylogenomic databases
eggNOG	NOG274661.
GeneTree	ENSGT00630000089736.
HOGENOM	HOG000230485.
HOVERGEN	HBG106919.
InParanoid	P30680.
KO	K04218.
OMA	STVVITC.
OrthoDB	EOG4PK283.
Gene expression databases
ArrayExpress	P30680.
Genevestigator	P30680.
GermOnline	ENSRNOG00000002793. Rattus norvegicus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000586. Somatstn_rcpt. IPR002074. Somatstn_rcpt_2. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00246. SOMATOSTATNR. PR00588. SOMATOSTTN2R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P30680.
ChEMBL	CHEMBL2978.
NextBio	610964.

Entry information

Entry name

SSR2_RAT

Accession

Primary (citable) accession number: P30680

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program