P30679 (GNA15_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein subunit alpha-15
Short name=G alpha-15
Short name=G-protein subunit alpha-15

Alternative name(s):
Epididymis tissue protein Li 17E
Guanine nucleotide-binding protein subunit alpha-16
Short name=G alpha-16
Short name=G-protein subunit alpha-16

Gene names

Name:	GNA15
Synonyms:	GNA16

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
Subunit structure	G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Tissue specificity	Specifically expressed in hematopoietic cells. Expressed in epididymis (at protein level). Ref.2
Sequence similarities	Belongs to the G-alpha family. G(q) subfamily.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transducer
PTM	ADP-ribosylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of phospholipase C activity Traceable author statement PubMed 7797501. Source: ProtInc adenylate cyclase-modulating G-protein coupled receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome elevation of cytosolic calcium ion concentration Inferred from genetic interaction PubMed 10571060. Source: UniProtKB phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Traceable author statement PubMed 7797501. Source: ProtInc phospholipase C-activating dopamine receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome platelet activation Traceable author statement. Source: Reactome
Cellular_component	heterotrimeric G-protein complex Non-traceable author statement PubMed 10571060. Source: UniProtKB
Molecular_function	G-protein beta/gamma-subunit complex binding Inferred from Biological aspect of Ancestor. Source: RefGenome G-protein coupled receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome GTP binding Non-traceable author statement. Source: UniProtKB GTPase activity Traceable author statement PubMed 7797501. Source: ProtInc metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from genetic interaction PubMed 10571060. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Guanine nucleotide-binding protein subunit alpha-15		PRO_0000203755
Regions
	Nucleotide binding	49 – 56	8	GTP By similarity
	Nucleotide binding	183 – 189	7	GTP By similarity
	Nucleotide binding	208 – 212	5	GTP By similarity
	Nucleotide binding	277 – 280	4	GTP By similarity
Sites
	Metal binding	56	1	Magnesium By similarity
	Metal binding	189	1	Magnesium By similarity
	Binding site	346	1	GTP; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	186	1	ADP-ribosylarginine; by cholera toxin By similarity
Natural variations
	Natural variant	147	1	Y → C. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.7 Corresponds to variant rs310680 [ dbSNP | Ensembl ].		VAR_028000

Sequences

	Sequence		Length	Mass (Da)
	P30679 [UniParc]. Last modified November 2, 2010. Version 2. Checksum: F32F8C9198FDC26A	FASTA	374	43,568
	10 20 30 40 50 60 MARSLTWRCC PWCLTEDEKA AARVDQEINR ILLEQKKQDR GELKLLLLGP GESGKSTFIK 70 80 90 100 110 120 QMRIIHGAGY SEEERKGFRP LVYQNIFVSM RAMIEAMERL QIPFSRPESK HHASLVMSQD 130 140 150 160 170 180 PYKVTTFEKR YAAAMQWLWR DAGIRAYYER RREFHLLDSA VYYLSHLERI TEEGYVPTAQ 190 200 210 220 230 240 DVLRSRMPTT GINEYCFSVQ KTNLRIVDVG GQKSERKKWI HCFENVIALI YLASLSEYDQ 250 260 270 280 290 300 CLEENNQENR MKESLALFGT ILELPWFKST SVILFLNKTD ILEEKIPTSH LATYFPSFQG 310 320 330 340 350 360 PKQDAEAAKR FILDMYTRMY TGCVDGPEGS KKGARSRRLF SHYTCATDTQ NIRKVFKDVR 370 DSVLARYLDE INLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"G alpha 16, a G protein alpha subunit specifically expressed in hematopoietic cells." Amatruda T.T. III, Steele D.A., Slepak V.Z., Simon M.I. Proc. Natl. Acad. Sci. U.S.A. 88:5587-5591(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT CYS-147.
[2]	"Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins." Li J., Liu F., Wang H., Liu X., Liu J., Li N., Wan F., Wang W., Zhang C., Jin S., Liu J., Zhu P., Liu Y. Mol. Cell. Proteomics 9:2517-2528(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT CYS-147. Tissue: Epididymis.
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-147.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-147.
[5]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT CYS-147.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-147. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63904 mRNA. Translation: AAA35860.1. GU727637 mRNA. Translation: ADU87639.1. GU727645 mRNA. Translation: ADU87646.1. AF493904 mRNA. Translation: AAM12618.1. BT009850 mRNA. Translation: AAP88852.1. AC005264 Genomic DNA. Translation: AAC25612.1. AC005262 Genomic DNA. Translation: AAC25616.1. CH471139 Genomic DNA. Translation: EAW69338.1. BC013585 mRNA. Translation: AAH13585.1.
IPI	IPI00027243.
PIR	A41096.
RefSeq	NP_002059.2. NM_002068.2.
UniGene	Hs.73797.
3D structure databases
ProteinModelPortal	P30679.
SMR	P30679. Positions 17-364.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000262958.
PTM databases
PhosphoSite	P30679.
Polymorphism databases
DMDM	311033388.
Proteomic databases
PaxDb	P30679.
PRIDE	P30679.
Protocols and materials databases
DNASU	2769.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000262958; ENSP00000262958; ENSG00000060558.
GeneID	2769.
KEGG	hsa:2769.
UCSC	uc002lxf.2. human.
Organism-specific databases
CTD	2769.
GeneCards	GC19P003140.
HGNC	HGNC:4383. GNA15.
HPA	HPA043113.
MIM	139314. gene.
neXtProt	NX_P30679.
PharmGKB	PA28768.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG271464.
HOGENOM	HOG000038729.
HOVERGEN	HBG063184.
InParanoid	P30679.
KO	K04637.
OMA	NIFVSMQ.
OrthoDB	EOG4Z8XWS.
PhylomeDB	P30679.
Enzyme and pathway databases
Pathway_Interaction_DB	arf6cyclingpathway. Arf6 signaling events. endothelinpathway. Endothelins. lysophospholipid_pathway. LPA receptor mediated events. er_nongenomic_pathway. Plasma membrane estrogen receptor signaling. s1p_s1p2_pathway. S1P2 pathway. s1p_s1p3_pathway. S1P3 pathway. s1p_meta_pathway. Sphingosine 1-phosphate (S1P) pathway. txa2pathway. Thromboxane A2 receptor signaling.
Reactome	REACT_111102. Signal Transduction. REACT_111217. Metabolism. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	P30679.
Bgee	P30679.
CleanEx	HS_GNA15.
Genevestigator	P30679.
GermOnline	ENSG00000060558. Homo sapiens.
Family and domain databases
Gene3D	1.10.400.10. 1 hit.
InterPro	IPR000654. Gprotein_alpha_Q. IPR001019. Gprotein_alpha_su. IPR011025. GproteinA_insert. IPR027417. P-loop_NTPase. [Graphical view]
PANTHER	PTHR10218. PTHR10218. 1 hit.
Pfam	PF00503. G-alpha. 1 hit. [Graphical view]
PRINTS	PR00318. GPROTEINA. PR00442. GPROTEINAQ.
SMART	SM00275. G_alpha. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit. SSF47895. Transducn_insert. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	2769.
NextBio	10892.
SOURCE	Search...

Entry information

Entry name

GNA15_HUMAN

Accession

Primary (citable) accession number: P30679
Secondary accession number(s): E9KL40 , E9KL47, O75247, Q53XK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 2, 2010
Last modified:	May 29, 2013
This is version 138 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.