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P30679 (GNA15_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine nucleotide-binding protein subunit alpha-15

Short name=G alpha-15
Short name=G-protein subunit alpha-15
Alternative name(s):
Epididymis tissue protein Li 17E
Guanine nucleotide-binding protein subunit alpha-16
Short name=G alpha-16
Short name=G-protein subunit alpha-16
Gene names
Name:GNA15
Synonyms:GNA16
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Subunit structure

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Tissue specificity

Specifically expressed in hematopoietic cells. Expressed in epididymis (at protein level). Ref.2

Sequence similarities

Belongs to the G-alpha family. G(q) subfamily.

Ontologies

Keywords
   Coding sequence diversityPolymorphism
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionTransducer
   PTMADP-ribosylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of phospholipase C activity

Traceable author statement PubMed 7797501. Source: ProtInc

adenylate cyclase-modulating G-protein coupled receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

blood coagulation

Traceable author statement. Source: Reactome

phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway

Traceable author statement PubMed 7797501. Source: ProtInc

phospholipase C-activating dopamine receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

platelet activation

Traceable author statement. Source: Reactome

positive regulation of cytosolic calcium ion concentration

Inferred from genetic interaction PubMed 10571060. Source: UniProtKB

   Cellular_componentextrinsic component of cytoplasmic side of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

heterotrimeric G-protein complex

Non-traceable author statement PubMed 10571060. Source: UniProtKB

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionG-protein beta/gamma-subunit complex binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

G-protein coupled receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

GTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Traceable author statement PubMed 7797501. Source: ProtInc

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

signal transducer activity

Inferred from genetic interaction PubMed 10571060. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374Guanine nucleotide-binding protein subunit alpha-15
PRO_0000203755

Regions

Nucleotide binding49 – 568GTP By similarity
Nucleotide binding183 – 1897GTP By similarity
Nucleotide binding208 – 2125GTP By similarity
Nucleotide binding277 – 2804GTP By similarity

Sites

Metal binding561Magnesium By similarity
Metal binding1891Magnesium By similarity
Binding site3461GTP; via amide nitrogen By similarity

Amino acid modifications

Modified residue1861ADP-ribosylarginine; by cholera toxin By similarity

Natural variations

Natural variant1471Y → C. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.7
Corresponds to variant rs310680 [ dbSNP | Ensembl ].
VAR_028000

Sequences

Sequence LengthMass (Da)Tools
P30679 [UniParc].

Last modified November 2, 2010. Version 2.
Checksum: F32F8C9198FDC26A

FASTA37443,568
        10         20         30         40         50         60 
MARSLTWRCC PWCLTEDEKA AARVDQEINR ILLEQKKQDR GELKLLLLGP GESGKSTFIK 

        70         80         90        100        110        120 
QMRIIHGAGY SEEERKGFRP LVYQNIFVSM RAMIEAMERL QIPFSRPESK HHASLVMSQD 

       130        140        150        160        170        180 
PYKVTTFEKR YAAAMQWLWR DAGIRAYYER RREFHLLDSA VYYLSHLERI TEEGYVPTAQ 

       190        200        210        220        230        240 
DVLRSRMPTT GINEYCFSVQ KTNLRIVDVG GQKSERKKWI HCFENVIALI YLASLSEYDQ 

       250        260        270        280        290        300 
CLEENNQENR MKESLALFGT ILELPWFKST SVILFLNKTD ILEEKIPTSH LATYFPSFQG 

       310        320        330        340        350        360 
PKQDAEAAKR FILDMYTRMY TGCVDGPEGS KKGARSRRLF SHYTCATDTQ NIRKVFKDVR 

       370 
DSVLARYLDE INLL 

« Hide

References

« Hide 'large scale' references
[1]"G alpha 16, a G protein alpha subunit specifically expressed in hematopoietic cells."
Amatruda T.T. III, Steele D.A., Slepak V.Z., Simon M.I.
Proc. Natl. Acad. Sci. U.S.A. 88:5587-5591(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT CYS-147.
[2]"Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins."
Li J., Liu F., Wang H., Liu X., Liu J., Li N., Wan F., Wang W., Zhang C., Jin S., Liu J., Zhu P., Liu Y.
Mol. Cell. Proteomics 9:2517-2528(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT CYS-147.
Tissue: Epididymis.
[3]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-147.
[4]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-147.
[5]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT CYS-147.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-147.
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63904 mRNA. Translation: AAA35860.1.
GU727637 mRNA. Translation: ADU87639.1.
GU727645 mRNA. Translation: ADU87646.1.
AF493904 mRNA. Translation: AAM12618.1.
BT009850 mRNA. Translation: AAP88852.1.
AC005264 Genomic DNA. Translation: AAC25612.1.
AC005262 Genomic DNA. Translation: AAC25616.1.
CH471139 Genomic DNA. Translation: EAW69338.1.
BC013585 mRNA. Translation: AAH13585.1.
PIRA41096.
RefSeqNP_002059.2. NM_002068.2.
UniGeneHs.73797.

3D structure databases

ProteinModelPortalP30679.
SMRP30679. Positions 21-364.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109031. 5 interactions.
STRING9606.ENSP00000262958.

PTM databases

PhosphoSiteP30679.

Polymorphism databases

DMDM311033388.

Proteomic databases

PaxDbP30679.
PRIDEP30679.

Protocols and materials databases

DNASU2769.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000262958; ENSP00000262958; ENSG00000060558.
GeneID2769.
KEGGhsa:2769.
UCSCuc002lxf.2. human.

Organism-specific databases

CTD2769.
GeneCardsGC19P003140.
HGNCHGNC:4383. GNA15.
HPAHPA043113.
MIM139314. gene.
neXtProtNX_P30679.
PharmGKBPA28768.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG271464.
HOGENOMHOG000038729.
HOVERGENHBG063184.
InParanoidP30679.
KOK04637.
OMANIFVSMQ.
OrthoDBEOG7ZWD1W.
PhylomeDBP30679.
TreeFamTF300673.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_111217. Metabolism.
REACT_604. Hemostasis.

Gene expression databases

ArrayExpressP30679.
BgeeP30679.
CleanExHS_GNA15.
GenevestigatorP30679.

Family and domain databases

Gene3D1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR000654. Gprotein_alpha_Q.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10218. PTHR10218. 1 hit.
PfamPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSPR00318. GPROTEINA.
PR00442. GPROTEINAQ.
SMARTSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

GenomeRNAi2769.
NextBio10892.
PROP30679.
SOURCESearch...

Entry information

Entry nameGNA15_HUMAN
AccessionPrimary (citable) accession number: P30679
Secondary accession number(s): E9KL40 expand/collapse secondary AC list , E9KL47, O75247, Q53XK2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 2, 2010
Last modified: April 16, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM