Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P30679

- GNA15_HUMAN

UniProt

P30679 - GNA15_HUMAN

Protein

Guanine nucleotide-binding protein subunit alpha-15

Gene

GNA15

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 2 (02 Nov 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi56 – 561MagnesiumBy similarity
    Metal bindingi189 – 1891MagnesiumBy similarity
    Binding sitei346 – 3461GTP; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi49 – 568GTPBy similarity
    Nucleotide bindingi183 – 1897GTPBy similarity
    Nucleotide bindingi208 – 2125GTPBy similarity
    Nucleotide bindingi277 – 2804GTPBy similarity

    GO - Molecular functioni

    1. G-protein beta/gamma-subunit complex binding Source: RefGenome
    2. G-protein coupled receptor binding Source: RefGenome
    3. GTPase activity Source: RefGenome
    4. GTP binding Source: UniProtKB-KW
    5. metal ion binding Source: UniProtKB-KW
    6. signal transducer activity Source: UniProtKB

    GO - Biological processi

    1. activation of phospholipase C activity Source: ProtInc
    2. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RefGenome
    3. blood coagulation Source: Reactome
    4. phospholipase C-activating dopamine receptor signaling pathway Source: RefGenome
    5. phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
    6. platelet activation Source: Reactome
    7. positive regulation of cytosolic calcium ion concentration Source: UniProtKB

    Keywords - Molecular functioni

    Transducer

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_18283. G alpha (q) signalling events.
    REACT_18405. Acetylcholine regulates insulin secretion.
    REACT_19140. ADP signalling through P2Y purinoceptor 1.
    REACT_19193. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
    REACT_20647. Thromboxane signalling through TP receptor.
    REACT_21384. Thrombin signalling through proteinase activated receptors (PARs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein subunit alpha-15
    Short name:
    G alpha-15
    Short name:
    G-protein subunit alpha-15
    Alternative name(s):
    Epididymis tissue protein Li 17E
    Guanine nucleotide-binding protein subunit alpha-16
    Short name:
    G alpha-16
    Short name:
    G-protein subunit alpha-16
    Gene namesi
    Name:GNA15
    Synonyms:GNA16
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:4383. GNA15.

    Subcellular locationi

    GO - Cellular componenti

    1. heterotrimeric G-protein complex Source: UniProtKB
    2. plasma membrane Source: Reactome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28768.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 374374Guanine nucleotide-binding protein subunit alpha-15PRO_0000203755Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei186 – 1861ADP-ribosylarginine; by cholera toxinBy similarity

    Keywords - PTMi

    ADP-ribosylation

    Proteomic databases

    MaxQBiP30679.
    PaxDbiP30679.
    PRIDEiP30679.

    PTM databases

    PhosphoSiteiP30679.

    Expressioni

    Tissue specificityi

    Specifically expressed in hematopoietic cells. Expressed in epididymis (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiP30679.
    BgeeiP30679.
    CleanExiHS_GNA15.
    GenevestigatoriP30679.

    Organism-specific databases

    HPAiHPA043113.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

    Protein-protein interaction databases

    BioGridi109031. 5 interactions.
    IntActiP30679. 9 interactions.
    STRINGi9606.ENSP00000262958.

    Structurei

    3D structure databases

    ProteinModelPortaliP30679.
    SMRiP30679. Positions 21-364.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-alpha family. G(q) subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG271464.
    HOGENOMiHOG000038729.
    HOVERGENiHBG063184.
    InParanoidiP30679.
    KOiK04637.
    OMAiNIFVSMQ.
    OrthoDBiEOG7ZWD1W.
    PhylomeDBiP30679.
    TreeFamiTF300673.

    Family and domain databases

    Gene3Di1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR000654. Gprotein_alpha_Q.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10218. PTHR10218. 1 hit.
    PfamiPF00503. G-alpha. 1 hit.
    [Graphical view]
    PRINTSiPR00318. GPROTEINA.
    PR00442. GPROTEINAQ.
    SMARTiSM00275. G_alpha. 1 hit.
    [Graphical view]
    SUPFAMiSSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P30679-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARSLTWRCC PWCLTEDEKA AARVDQEINR ILLEQKKQDR GELKLLLLGP    50
    GESGKSTFIK QMRIIHGAGY SEEERKGFRP LVYQNIFVSM RAMIEAMERL 100
    QIPFSRPESK HHASLVMSQD PYKVTTFEKR YAAAMQWLWR DAGIRAYYER 150
    RREFHLLDSA VYYLSHLERI TEEGYVPTAQ DVLRSRMPTT GINEYCFSVQ 200
    KTNLRIVDVG GQKSERKKWI HCFENVIALI YLASLSEYDQ CLEENNQENR 250
    MKESLALFGT ILELPWFKST SVILFLNKTD ILEEKIPTSH LATYFPSFQG 300
    PKQDAEAAKR FILDMYTRMY TGCVDGPEGS KKGARSRRLF SHYTCATDTQ 350
    NIRKVFKDVR DSVLARYLDE INLL 374
    Length:374
    Mass (Da):43,568
    Last modified:November 2, 2010 - v2
    Checksum:iF32F8C9198FDC26A
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti147 – 1471Y → C.6 Publications
    Corresponds to variant rs310680 [ dbSNP | Ensembl ].
    VAR_028000

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63904 mRNA. Translation: AAA35860.1.
    GU727637 mRNA. Translation: ADU87639.1.
    GU727645 mRNA. Translation: ADU87646.1.
    AF493904 mRNA. Translation: AAM12618.1.
    BT009850 mRNA. Translation: AAP88852.1.
    AC005264 Genomic DNA. Translation: AAC25612.1.
    AC005262 Genomic DNA. Translation: AAC25616.1.
    CH471139 Genomic DNA. Translation: EAW69338.1.
    BC013585 mRNA. Translation: AAH13585.1.
    CCDSiCCDS12104.1.
    PIRiA41096.
    RefSeqiNP_002059.3. NM_002068.3.
    UniGeneiHs.73797.

    Genome annotation databases

    EnsembliENST00000262958; ENSP00000262958; ENSG00000060558.
    GeneIDi2769.
    KEGGihsa:2769.
    UCSCiuc002lxf.2. human.

    Polymorphism databases

    DMDMi311033388.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63904 mRNA. Translation: AAA35860.1 .
    GU727637 mRNA. Translation: ADU87639.1 .
    GU727645 mRNA. Translation: ADU87646.1 .
    AF493904 mRNA. Translation: AAM12618.1 .
    BT009850 mRNA. Translation: AAP88852.1 .
    AC005264 Genomic DNA. Translation: AAC25612.1 .
    AC005262 Genomic DNA. Translation: AAC25616.1 .
    CH471139 Genomic DNA. Translation: EAW69338.1 .
    BC013585 mRNA. Translation: AAH13585.1 .
    CCDSi CCDS12104.1.
    PIRi A41096.
    RefSeqi NP_002059.3. NM_002068.3.
    UniGenei Hs.73797.

    3D structure databases

    ProteinModelPortali P30679.
    SMRi P30679. Positions 21-364.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109031. 5 interactions.
    IntActi P30679. 9 interactions.
    STRINGi 9606.ENSP00000262958.

    PTM databases

    PhosphoSitei P30679.

    Polymorphism databases

    DMDMi 311033388.

    Proteomic databases

    MaxQBi P30679.
    PaxDbi P30679.
    PRIDEi P30679.

    Protocols and materials databases

    DNASUi 2769.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000262958 ; ENSP00000262958 ; ENSG00000060558 .
    GeneIDi 2769.
    KEGGi hsa:2769.
    UCSCi uc002lxf.2. human.

    Organism-specific databases

    CTDi 2769.
    GeneCardsi GC19P003140.
    HGNCi HGNC:4383. GNA15.
    HPAi HPA043113.
    MIMi 139314. gene.
    neXtProti NX_P30679.
    PharmGKBi PA28768.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG271464.
    HOGENOMi HOG000038729.
    HOVERGENi HBG063184.
    InParanoidi P30679.
    KOi K04637.
    OMAi NIFVSMQ.
    OrthoDBi EOG7ZWD1W.
    PhylomeDBi P30679.
    TreeFami TF300673.

    Enzyme and pathway databases

    Reactomei REACT_18283. G alpha (q) signalling events.
    REACT_18405. Acetylcholine regulates insulin secretion.
    REACT_19140. ADP signalling through P2Y purinoceptor 1.
    REACT_19193. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
    REACT_20647. Thromboxane signalling through TP receptor.
    REACT_21384. Thrombin signalling through proteinase activated receptors (PARs).

    Miscellaneous databases

    GenomeRNAii 2769.
    NextBioi 10892.
    PROi P30679.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P30679.
    Bgeei P30679.
    CleanExi HS_GNA15.
    Genevestigatori P30679.

    Family and domain databases

    Gene3Di 1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR000654. Gprotein_alpha_Q.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10218. PTHR10218. 1 hit.
    Pfami PF00503. G-alpha. 1 hit.
    [Graphical view ]
    PRINTSi PR00318. GPROTEINA.
    PR00442. GPROTEINAQ.
    SMARTi SM00275. G_alpha. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "G alpha 16, a G protein alpha subunit specifically expressed in hematopoietic cells."
      Amatruda T.T. III, Steele D.A., Slepak V.Z., Simon M.I.
      Proc. Natl. Acad. Sci. U.S.A. 88:5587-5591(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT CYS-147.
    2. "Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins."
      Li J., Liu F., Wang H., Liu X., Liu J., Li N., Wan F., Wang W., Zhang C., Jin S., Liu J., Zhu P., Liu Y.
      Mol. Cell. Proteomics 9:2517-2528(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT CYS-147.
      Tissue: Epididymis.
    3. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-147.
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-147.
    5. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT CYS-147.
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-147.
      Tissue: Lung.

    Entry informationi

    Entry nameiGNA15_HUMAN
    AccessioniPrimary (citable) accession number: P30679
    Secondary accession number(s): E9KL40
    , E9KL47, O75247, Q53XK2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: November 2, 2010
    Last modified: October 1, 2014
    This is version 149 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3