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Protein

Guanine nucleotide-binding protein subunit alpha-15

Gene

Gna15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi56MagnesiumBy similarity1
Metal bindingi189MagnesiumBy similarity1
Binding sitei346GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 56GTPBy similarity8
Nucleotide bindingi183 – 189GTPBy similarity7
Nucleotide bindingi208 – 212GTPBy similarity5
Nucleotide bindingi277 – 280GTPBy similarity4

GO - Molecular functioni

  • G-protein beta/gamma-subunit complex binding Source: GO_Central
  • G-protein coupled receptor binding Source: GO_Central
  • GTPase activity Source: MGI
  • GTP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • signal transducer activity Source: UniProtKB

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: GO_Central
  • G-protein coupled receptor signaling pathway Source: MGI
  • phospholipase C-activating dopamine receptor signaling pathway Source: GO_Central
  • phospholipase C-activating G-protein coupled receptor signaling pathway Source: MGI
  • positive regulation of cytosolic calcium ion concentration Source: UniProtKB

Keywordsi

Molecular functionTransducer
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-399997. Acetylcholine regulates insulin secretion.
R-MMU-416476. G alpha (q) signalling events.
R-MMU-418592. ADP signalling through P2Y purinoceptor 1.
R-MMU-428930. Thromboxane signalling through TP receptor.
R-MMU-432142. Platelet sensitization by LDL.
R-MMU-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-MMU-456926. Thrombin signalling through proteinase activated receptors (PARs).

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit alpha-15
Short name:
G alpha-15
Short name:
G-protein subunit alpha-15
Gene namesi
Name:Gna15
Synonyms:Gna-15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:95770. Gna15.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002037561 – 374Guanine nucleotide-binding protein subunit alpha-15Add BLAST374

Proteomic databases

MaxQBiP30678.
PaxDbiP30678.
PRIDEiP30678.

PTM databases

iPTMnetiP30678.
PhosphoSitePlusiP30678.

Expressioni

Tissue specificityi

Expressed primarily in hematopoietic cells. Coexpressed with EDG6 at the same relative levels in all tissues examined, with the highest levels in adult spleen and lung.1 Publication

Gene expression databases

BgeeiENSMUSG00000034792.
CleanExiMM_GNA15.
GenevisibleiP30678. MM.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049175.

Structurei

3D structure databases

ProteinModelPortaliP30678.
SMRiP30678.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(q) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0085. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP30678.
KOiK04637.
OMAiWRCCPWC.
OrthoDBiEOG091G0VUT.
PhylomeDBiP30678.
TreeFamiTF300673.

Family and domain databases

CDDicd00066. G-alpha. 1 hit.
Gene3Di1.10.400.10. 1 hit.
InterProiView protein in InterPro
IPR000654. Gprotein_alpha_Q.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiView protein in Pfam
PF00503. G-alpha. 1 hit.
PRINTSiPR00318. GPROTEINA.
PR00442. GPROTEINAQ.
SMARTiView protein in SMART
SM00275. G_alpha. 1 hit.
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

P30678-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSLTWGCC PWCLTEEEKT AARIDQEINR ILLEQKKQER EELKLLLLGP
60 70 80 90 100
GESGKSTFIK QMRIIHGVGY SEEDRRAFRL LIYQNIFVSM QAMIDAMDRL
110 120 130 140 150
QIPFSRPDSK QHASLVMTQD PYKVSTFEKP YAVAMQYLWR DAGIRACYER
160 170 180 190 200
RREFHLLDSA VYYLSHLERI SEDSYIPTAQ DVLRSRMPTT GINEYCFSVK
210 220 230 240 250
KTKLRIVDVG GQRSERRKWI HCFENVIALI YLASLSEYDQ CLEENDQENR
260 270 280 290 300
MEESLALFST ILELPWFKST SVILFLNKTD ILEDKIHTSH LATYFPSFQG
310 320 330 340 350
PRRDAEAAKS FILDMYARVY ASCAEPQDGG RKGSRARRFF AHFTCATDTQ
360 370
SVRSVFKDVR DSVLARYLDE INLL
Length:374
Mass (Da):43,536
Last modified:April 1, 1993 - v1
Checksum:i44F15DC52C8C233E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80632 mRNA. Translation: AAA37713.1.
U37419
, U37414, U37415, U37416, U37417, U37418 Genomic DNA. Translation: AAB36840.1.
BC005439 mRNA. Translation: AAH05439.1.
BC011098 mRNA. Translation: AAH11098.1.
AJ489247 Genomic DNA. Translation: CAD33253.1.
CCDSiCCDS24060.1.
PIRiB41534.
RefSeqiNP_034434.1. NM_010304.3.
UniGeneiMm.1546.

Genome annotation databases

EnsembliENSMUST00000043709; ENSMUSP00000049175; ENSMUSG00000034792.
GeneIDi14676.
KEGGimmu:14676.
UCSCiuc007gij.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGNA15_MOUSE
AccessioniPrimary (citable) accession number: P30678
Secondary accession number(s): Q8K1S0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 5, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families