P30676 (GNAI_ASTPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Guanine nucleotide-binding protein G(i) subunit alpha Alternative name(s): Adenylate cyclase-inhibiting G alpha protein
Organism	Asterina pectinifera (Starfish) (Patiria pectinifera)
Taxonomic identifier	7594 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Echinodermata › Eleutherozoa › Asterozoa › Asteroidea › Valvatacea › Valvatida › Asterinidae › Patiria

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. This G protein is involved in 1-methyladenine-induced oocyte maturation.
Subunit structure	G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Sequence similarities	Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Ontologies

Keywords
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transducer
PTM	Lipoprotein Myristate Palmitate
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	adenylate cyclase-modulating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 354	353	Guanine nucleotide-binding protein G(i) subunit alpha		PRO_0000203685
Regions
	Nucleotide binding	40 – 47	8	GTP By similarity
	Nucleotide binding	175 – 181	7	GTP By similarity
	Nucleotide binding	200 – 204	5	GTP By similarity
	Nucleotide binding	269 – 272	4	GTP By similarity
Sites
	Metal binding	47	1	Magnesium By similarity
	Metal binding	181	1	Magnesium By similarity
	Binding site	326	1	GTP; via amide nitrogen By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Potential
	Lipidation	3	1	S-palmitoyl cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	P30676 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E242F5BB911B05E1	FASTA	354	40,043
	10 20 30 40 50 60 MGCATSAEDK AAAERSKAID RNLRIDGEKA AREVKLLLLG AGESGKSTIV KQMKIIHEEG 70 80 90 100 110 120 YSEEDCKQYK PVVYSNTIQS MIAIIRAMGS LKVDFGDGDR TDDARQLFAL AGQAEEGELS 130 140 150 160 170 180 PELAAVMKRL WADGGVQGCF SRSREYQLND SASYYLNALD RLAAPGYIPT QQDVLRTRVK 190 200 210 220 230 240 TTGIVETHFT FKDLHFKMFD VGGQRSERKK WIHCFEGVTA IIFCVALSAY DLVLAEDEEM 250 260 270 280 290 300 NRMHESMKLF DSICNNKWFT ETSIILFLNK KDLFEEKITK SPLTICFPEY TGSNTYEEAA 310 320 330 340 350 AYIQMQFEDL NKRKDQKEIY THFTCATDTN NIQFVFDAVT DVIIKNNLKD CGLF

References

[1]

"The primary structure of the alpha subunit of a starfish guanosine-nucleotide-binding regulatory protein involved in 1-methyladenine-induced oocyte maturation." Chiba K., Tadenuma H., Matsumoto M., Takahashi K., Katada T., Hoshi M. Eur. J. Biochem. 207:833-838(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X66378 mRNA. Translation: CAA47019.1.
PIR	S24362.
3D structure databases
ProteinModelPortal	P30676.
SMR	P30676. Positions 6-353.
ModBase	Search...
Proteomic databases
PRIDE	P30676.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.400.10. 1 hit.
InterPro	IPR001408. Gprotein_alpha_I. IPR001019. Gprotein_alpha_su. IPR011025. GproteinA_insert. [Graphical view]
PANTHER	PTHR10218. PTHR10218. 1 hit.
Pfam	PF00503. G-alpha. 1 hit. [Graphical view]
PRINTS	PR00318. GPROTEINA. PR00441. GPROTEINAI.
SMART	SM00275. G_alpha. 1 hit. [Graphical view]
SUPFAM	SSF47895. Transducn_insert. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GNAI_ASTPE

Accession

Primary (citable) accession number: P30676

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)