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P30676

- GNAI_PATPE

UniProt

P30676 - GNAI_PATPE

Protein

Guanine nucleotide-binding protein G(i) subunit alpha

Gene
N/A
Organism
Patiria pectinifera (Starfish) (Asterina pectinifera)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. This G protein is involved in 1-methyladenine-induced oocyte maturation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471MagnesiumBy similarity
    Metal bindingi181 – 1811MagnesiumBy similarity
    Binding sitei326 – 3261GTP; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi40 – 478GTPBy similarity
    Nucleotide bindingi175 – 1817GTPBy similarity
    Nucleotide bindingi200 – 2045GTPBy similarity
    Nucleotide bindingi269 – 2724GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Transducer

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein G(i) subunit alpha
    Alternative name(s):
    Adenylate cyclase-inhibiting G alpha protein
    OrganismiPatiria pectinifera (Starfish) (Asterina pectinifera)
    Taxonomic identifieri7594 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEchinodermataEleutherozoaAsterozoaAsteroideaValvataceaValvatidaAsterinidaePatiria

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 354353Guanine nucleotide-binding protein G(i) subunit alphaPRO_0000203685Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineSequence Analysis
    Lipidationi3 – 31S-palmitoyl cysteineSequence Analysis

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate

    Proteomic databases

    PRIDEiP30676.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

    Structurei

    3D structure databases

    ProteinModelPortaliP30676.
    SMRiP30676. Positions 6-353.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

    Family and domain databases

    Gene3Di1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR001408. Gprotein_alpha_I.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10218. PTHR10218. 1 hit.
    PfamiPF00503. G-alpha. 1 hit.
    [Graphical view]
    PRINTSiPR00318. GPROTEINA.
    PR00441. GPROTEINAI.
    SMARTiSM00275. G_alpha. 1 hit.
    [Graphical view]
    SUPFAMiSSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30676-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCATSAEDK AAAERSKAID RNLRIDGEKA AREVKLLLLG AGESGKSTIV    50
    KQMKIIHEEG YSEEDCKQYK PVVYSNTIQS MIAIIRAMGS LKVDFGDGDR 100
    TDDARQLFAL AGQAEEGELS PELAAVMKRL WADGGVQGCF SRSREYQLND 150
    SASYYLNALD RLAAPGYIPT QQDVLRTRVK TTGIVETHFT FKDLHFKMFD 200
    VGGQRSERKK WIHCFEGVTA IIFCVALSAY DLVLAEDEEM NRMHESMKLF 250
    DSICNNKWFT ETSIILFLNK KDLFEEKITK SPLTICFPEY TGSNTYEEAA 300
    AYIQMQFEDL NKRKDQKEIY THFTCATDTN NIQFVFDAVT DVIIKNNLKD 350
    CGLF 354
    Length:354
    Mass (Da):40,043
    Last modified:January 23, 2007 - v3
    Checksum:iE242F5BB911B05E1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66378 mRNA. Translation: CAA47019.1.
    PIRiS24362.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66378 mRNA. Translation: CAA47019.1 .
    PIRi S24362.

    3D structure databases

    ProteinModelPortali P30676.
    SMRi P30676. Positions 6-353.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P30676.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR001408. Gprotein_alpha_I.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10218. PTHR10218. 1 hit.
    Pfami PF00503. G-alpha. 1 hit.
    [Graphical view ]
    PRINTSi PR00318. GPROTEINA.
    PR00441. GPROTEINAI.
    SMARTi SM00275. G_alpha. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The primary structure of the alpha subunit of a starfish guanosine-nucleotide-binding regulatory protein involved in 1-methyladenine-induced oocyte maturation."
      Chiba K., Tadenuma H., Matsumoto M., Takahashi K., Katada T., Hoshi M.
      Eur. J. Biochem. 207:833-838(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Ovary.

    Entry informationi

    Entry nameiGNAI_PATPE
    AccessioniPrimary (citable) accession number: P30676
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 83 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3