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Protein

DNA replication licensing factor mcm3

Gene

mcm3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi356 – 363ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-68962 Activation of the pre-replicative complex
R-SPO-69052 Switching of origins to a post-replicative state
R-SPO-69300 Removal of licensing factors from origins

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor mcm3 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 3
Gene namesi
Name:mcm3
ORF Names:SPCC1682.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC1682.02c
PomBaseiSPCC1682.02c mcm3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941001 – 879DNA replication licensing factor mcm3Add BLAST879

Proteomic databases

MaxQBiP30666
PaxDbiP30666
PRIDEiP30666

PTM databases

SwissPalmiP30666

Interactioni

Subunit structurei

Component of the mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (Probable). The heterodimers of mcm4/mcm6 and mcm3/mcm5 interact with mcm2 and mcm7.Curated1 Publication

Protein-protein interaction databases

BioGridi275419, 11 interactors
IntActiP30666, 4 interactors
MINTiP30666
STRINGi4896.SPCC1682.02c.1

Structurei

3D structure databases

ProteinModelPortaliP30666
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini306 – 513MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi488 – 491Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

HOGENOMiHOG000224126
InParanoidiP30666
KOiK02541
OMAiYQSKVRE
OrthoDBiEOG092C0VUM
PhylomeDBiP30666

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031327 MCM
IPR008046 Mcm3
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01659 MCMPROTEIN3
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P30666-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELLADEVF KDRVRIFQEY LEHDTDDANV TLYQEAILRM LNMGQRRLIV
60 70 80 90 100
NIDELRDYNR ELADGVLLKP LEYVEPFDEA LRNVVSTLID PVVHKDLKDK
110 120 130 140 150
LFYVGFRGSF GDHHVNPRTL RAMHLNKMIS LEGIVTRCSF VRPKVIKSVH
160 170 180 190 200
YCEATKRHHF KQYADATMNG GLSFQSTVYP TQDENGNPLS IEFGFSTFRD
210 220 230 240 250
HQSISLQEMP ERAPPGQLPR SIDILLDDDL VDTVKPGDRV NIVGQYRSMG
260 270 280 290 300
SKTSGNTSAT FRTVLLANNV VLLGNKPGLG NVGGGALDIT DADIRNINKL
310 320 330 340 350
ARKKNVFELL STSLAPSIYG YEYVKQAILL LLLGGTEKNL TNGTHIRGDI
360 370 380 390 400
NILMVGDPST AKSQLLRFVL NTAPLAIATT GRGSSGVGLT AAVTTDKETG
410 420 430 440 450
ERRLEAGAMV LADRGVVCID EFDKMSDIDR VAIHEVMEQQ TVTIAKAGIH
460 470 480 490 500
TSLNARCSVI AAANPIYGQY DIRKDPHQNI ALPDSMLSRF DLLFIVTDDI
510 520 530 540 550
DDKKDRALSE HVLRMHRYLP PGVEPGTPVR DSLNSVLNVG ATNAAGVSTE
560 570 580 590 600
NVEQEVETPV WETFSSLLHA NARTKKKELL NINFVRKYIQ YAKSRIHPIL
610 620 630 640 650
NQATAEYITN IYCGLRNDDL QGNQRRTSPL TARTLETLIR LSTAHAKARL
660 670 680 690 700
SSVVEVKDAK AAEKILRYAL FREVVKPKRK KHKKQRLEAG EEFDSEDDNS
710 720 730 740 750
DDMDIEESEE EMDTNMVIDS GSRRVTRSQN ATSQSQESGS EIGSSIAGTA
760 770 780 790 800
GSYNVGTSNT QLSWPSTHST LPATSRELAS SDRNINTGTS VASEVSASVS
810 820 830 840 850
EQSTVSLPRE KMSVFMARLA SLTKSELFSE ECASLEDVLE SINNIEDDVG
860 870
FSREEAIVAL KEMDAQNKIM FSDNVVYRI
Length:879
Mass (Da):97,481
Last modified:December 15, 1998 - v2
Checksum:i876051CC7DE2504A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063864 Genomic DNA Translation: AAC32263.1
CU329672 Genomic DNA Translation: CAA20668.1
Z15034 Genomic DNA Translation: CAA78752.1
PIRiS26642
T41059
RefSeqiNP_587795.1, NM_001022788.2

Genome annotation databases

EnsemblFungiiSPCC1682.02c.1; SPCC1682.02c.1:pep; SPCC1682.02c
GeneIDi2538838
KEGGispo:SPCC1682.02c

Entry informationi

Entry nameiMCM3_SCHPO
AccessioniPrimary (citable) accession number: P30666
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: December 15, 1998
Last modified: March 28, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health