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Protein

DNA replication licensing factor mcm3

Gene

mcm3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi356 – 363ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • mitotic DNA replication initiation Source: PomBase
  • negative regulation of helicase activity Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-68962. Activation of the pre-replicative complex.
R-SPO-69052. Switching of origins to a post-replicative state.
R-SPO-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor mcm3 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 3
Gene namesi
Name:mcm3
ORF Names:SPCC1682.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC1682.02c.
PomBaseiSPCC1682.02c. mcm3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • MCM complex Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear replication fork Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941001 – 879DNA replication licensing factor mcm3Add BLAST879

Proteomic databases

MaxQBiP30666.
PRIDEiP30666.

PTM databases

SwissPalmiP30666.

Interactioni

Subunit structurei

Component of the mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (Probable). The heterodimers of mcm4/mcm6 and mcm3/mcm5 interact with mcm2 and mcm7.Curated1 Publication

Protein-protein interaction databases

BioGridi275419. 11 interactors.
IntActiP30666. 4 interactors.
MINTiMINT-4688246.

Structurei

3D structure databases

ProteinModelPortaliP30666.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini306 – 513MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi488 – 491Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

HOGENOMiHOG000224126.
InParanoidiP30666.
KOiK02541.
OMAiVDENRDR.
OrthoDBiEOG092C0VUM.
PhylomeDBiP30666.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30666-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELLADEVF KDRVRIFQEY LEHDTDDANV TLYQEAILRM LNMGQRRLIV
60 70 80 90 100
NIDELRDYNR ELADGVLLKP LEYVEPFDEA LRNVVSTLID PVVHKDLKDK
110 120 130 140 150
LFYVGFRGSF GDHHVNPRTL RAMHLNKMIS LEGIVTRCSF VRPKVIKSVH
160 170 180 190 200
YCEATKRHHF KQYADATMNG GLSFQSTVYP TQDENGNPLS IEFGFSTFRD
210 220 230 240 250
HQSISLQEMP ERAPPGQLPR SIDILLDDDL VDTVKPGDRV NIVGQYRSMG
260 270 280 290 300
SKTSGNTSAT FRTVLLANNV VLLGNKPGLG NVGGGALDIT DADIRNINKL
310 320 330 340 350
ARKKNVFELL STSLAPSIYG YEYVKQAILL LLLGGTEKNL TNGTHIRGDI
360 370 380 390 400
NILMVGDPST AKSQLLRFVL NTAPLAIATT GRGSSGVGLT AAVTTDKETG
410 420 430 440 450
ERRLEAGAMV LADRGVVCID EFDKMSDIDR VAIHEVMEQQ TVTIAKAGIH
460 470 480 490 500
TSLNARCSVI AAANPIYGQY DIRKDPHQNI ALPDSMLSRF DLLFIVTDDI
510 520 530 540 550
DDKKDRALSE HVLRMHRYLP PGVEPGTPVR DSLNSVLNVG ATNAAGVSTE
560 570 580 590 600
NVEQEVETPV WETFSSLLHA NARTKKKELL NINFVRKYIQ YAKSRIHPIL
610 620 630 640 650
NQATAEYITN IYCGLRNDDL QGNQRRTSPL TARTLETLIR LSTAHAKARL
660 670 680 690 700
SSVVEVKDAK AAEKILRYAL FREVVKPKRK KHKKQRLEAG EEFDSEDDNS
710 720 730 740 750
DDMDIEESEE EMDTNMVIDS GSRRVTRSQN ATSQSQESGS EIGSSIAGTA
760 770 780 790 800
GSYNVGTSNT QLSWPSTHST LPATSRELAS SDRNINTGTS VASEVSASVS
810 820 830 840 850
EQSTVSLPRE KMSVFMARLA SLTKSELFSE ECASLEDVLE SINNIEDDVG
860 870
FSREEAIVAL KEMDAQNKIM FSDNVVYRI
Length:879
Mass (Da):97,481
Last modified:December 15, 1998 - v2
Checksum:i876051CC7DE2504A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063864 Genomic DNA. Translation: AAC32263.1.
CU329672 Genomic DNA. Translation: CAA20668.1.
Z15034 Genomic DNA. Translation: CAA78752.1.
PIRiS26642.
T41059.
RefSeqiNP_587795.1. NM_001022788.2.

Genome annotation databases

EnsemblFungiiSPCC1682.02c.1; SPCC1682.02c.1:pep; SPCC1682.02c.
GeneIDi2538838.
KEGGispo:SPCC1682.02c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063864 Genomic DNA. Translation: AAC32263.1.
CU329672 Genomic DNA. Translation: CAA20668.1.
Z15034 Genomic DNA. Translation: CAA78752.1.
PIRiS26642.
T41059.
RefSeqiNP_587795.1. NM_001022788.2.

3D structure databases

ProteinModelPortaliP30666.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275419. 11 interactors.
IntActiP30666. 4 interactors.
MINTiMINT-4688246.

PTM databases

SwissPalmiP30666.

Proteomic databases

MaxQBiP30666.
PRIDEiP30666.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC1682.02c.1; SPCC1682.02c.1:pep; SPCC1682.02c.
GeneIDi2538838.
KEGGispo:SPCC1682.02c.

Organism-specific databases

EuPathDBiFungiDB:SPCC1682.02c.
PomBaseiSPCC1682.02c. mcm3.

Phylogenomic databases

HOGENOMiHOG000224126.
InParanoidiP30666.
KOiK02541.
OMAiVDENRDR.
OrthoDBiEOG092C0VUM.
PhylomeDBiP30666.

Enzyme and pathway databases

ReactomeiR-SPO-68962. Activation of the pre-replicative complex.
R-SPO-69052. Switching of origins to a post-replicative state.
R-SPO-69300. Removal of licensing factors from origins.

Miscellaneous databases

PROiP30666.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCM3_SCHPO
AccessioniPrimary (citable) accession number: P30666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: December 15, 1998
Last modified: November 30, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.