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Protein

Nitrogen fixation regulatory protein

Gene

nifL

Organism
Azotobacter vinelandii
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the inhibition of NifA activity in response to oxygen and low level of fixed nitrogen.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Redox potential

E0 is -226 mV.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processNitrogen fixation, Transcription, Transcription regulation, Two-component regulatory system
LigandATP-binding, FAD, Flavoprotein, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen fixation regulatory protein (EC:2.7.13.3)
Gene namesi
Name:nifL
OrganismiAzotobacter vinelandii
Taxonomic identifieri354 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000748151 – 519Nitrogen fixation regulatory proteinAdd BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei305Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP30663

Interactioni

Protein-protein interaction databases

IntActiP30663, 1 interactor

Structurei

Secondary structure

1519
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 33Combined sources10
Beta strandi35 – 41Combined sources7
Beta strandi46 – 50Combined sources5
Helixi52 – 58Combined sources7
Helixi64 – 67Combined sources4
Helixi70 – 73Combined sources4
Helixi80 – 91Combined sources12
Beta strandi96 – 103Combined sources8
Beta strandi109 – 120Combined sources12
Beta strandi126 – 135Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GJ3X-ray1.04A/B21-140[»]
ProteinModelPortaliP30663
SMRiP30663
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30663

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 93PAS 1PROSITE-ProRule annotationAdd BLAST71
Domaini94 – 148PACPROSITE-ProRule annotationAdd BLAST55
Domaini151 – 217PAS 2PROSITE-ProRule annotationAdd BLAST67
Domaini302 – 517Histidine kinasePROSITE-ProRule annotationAdd BLAST216

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105DS4 Bacteria
ENOG410XSW9 LUCA

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
cd00130 PAS, 2 hits
Gene3Di3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR014285 N_fixation_neg-reg_NifL
IPR001610 PAC
IPR000014 PAS
IPR000700 PAS-assoc_C
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR004358 Sig_transdc_His_kin-like_C
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00989 PAS, 2 hits
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SM00086 PAC, 1 hit
SM00091 PAS, 2 hits
SUPFAMiSSF55785 SSF55785, 2 hits
SSF55874 SSF55874, 1 hit
TIGRFAMsiTIGR02938 nifL_nitrog, 1 hit
TIGR00229 sensory_box, 1 hit
PROSITEiView protein in PROSITE
PS50109 HIS_KIN, 1 hit
PS50113 PAC, 1 hit
PS50112 PAS, 1 hit

Sequencei

Sequence statusi: Complete.

P30663-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPANPTLSN EPQAPHAESD ELLPEIFRQT VEHAPIAISI TDLKANILYA
60 70 80 90 100
NRAFRTITGY GSEEVLGKNE SILSNGTTPR LVYQALWGRL AQKKPWSGVL
110 120 130 140 150
VNRRKDKTLY LAELTVAPVL NEAGETIYYL GMHRDTSELH ELEQRVNNQR
160 170 180 190 200
LMIEAVVNAA PAAMVVLDRQ HRVMLSNPSF CRLARDLVED GSSESLVALL
210 220 230 240 250
RENLAAPFET LENQGSAFSG KEISFDLGGR SPRWLSCHGR AIHIENEQAH
260 270 280 290 300
VFFAPTEERY LLLTINDISE LRQKQQDSRL NALKALMAEE ELLEGMRETF
310 320 330 340 350
NAAIHRLQGP VNLISAAMRM LERRLGDKAG NDPVLSAMRE ASTAGMEALE
360 370 380 390 400
NLSGSIPVRM AESKMPVNLN QLIREVITLC TDQLLAQGIV VDWQPALRLP
410 420 430 440 450
WVMGGESSQR SMIKHLVDNA IESMSQNQVS RRELFISTRV ENHLVRMEIT
460 470 480 490 500
DSGPGIPPDL VLKVFEPFFS TKPPHRVGRG MGLPVVQEIV AKHAGMVHVD
510
TDYREGCRIV VELPFSAST
Length:519
Mass (Da):57,827
Last modified:February 1, 1996 - v2
Checksum:iA40B1E76BD3723CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64832 Genomic DNA Translation: CAA46044.1
Y00554 Genomic DNA Translation: CAA68632.1
PIRiS19883

Similar proteinsi

Entry informationi

Entry nameiNIFL_AZOVI
AccessioniPrimary (citable) accession number: P30663
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure
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Main funding by: National Institutes of Health