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P30663

- NIFL_AZOVI

UniProt

P30663 - NIFL_AZOVI

Protein

Nitrogen fixation regulatory protein

Gene

nifL

Organism
Azotobacter vinelandii
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Required for the inhibition of NifA activity in response to oxygen and low level of fixed nitrogen.

    Catalytic activityi

    ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

    Cofactori

    FAD.

    Redox potential

    E0 is -226 mV.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. flavin adenine dinucleotide binding Source: InterPro
    3. phosphorelay sensor kinase activity Source: InterPro

    GO - Biological processi

    1. nitrogen fixation Source: UniProtKB-KW
    2. regulation of transcription, DNA-templated Source: CACAO
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Nitrogen fixation, Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    ATP-binding, FAD, Flavoprotein, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrogen fixation regulatory protein (EC:2.7.13.3)
    Gene namesi
    Name:nifL
    OrganismiAzotobacter vinelandii
    Taxonomic identifieri354 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 519519Nitrogen fixation regulatory proteinPRO_0000074815Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei305 – 3051Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    IntActiP30663. 1 interaction.
    MINTiMINT-107527.

    Structurei

    Secondary structure

    1
    519
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi24 – 3310
    Beta strandi35 – 417
    Beta strandi46 – 505
    Helixi52 – 587
    Helixi64 – 674
    Helixi70 – 734
    Helixi80 – 9112
    Beta strandi96 – 1038
    Beta strandi109 – 12012
    Beta strandi126 – 13510

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2GJ3X-ray1.04A/B21-140[»]
    ProteinModelPortaliP30663.
    SMRiP30663. Positions 22-140.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP30663.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 9371PAS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini94 – 14855PACPROSITE-ProRule annotationAdd
    BLAST
    Domaini151 – 21767PAS 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini302 – 517216Histidine kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 histidine kinase domain.PROSITE-ProRule annotation
    Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
    Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di3.30.565.10. 1 hit.
    InterProiIPR003594. HATPase_ATP-bd.
    IPR014285. N_fixation_neg-reg_NifL.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR000700. PAS-assoc_C.
    IPR013767. PAS_fold.
    IPR004358. Sig_transdc_His_kin-like_C.
    IPR005467. Sig_transdc_His_kinase_core.
    [Graphical view]
    PfamiPF02518. HATPase_c. 1 hit.
    PF00989. PAS. 2 hits.
    [Graphical view]
    PRINTSiPR00344. BCTRLSENSOR.
    SMARTiSM00387. HATPase_c. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 2 hits.
    [Graphical view]
    SUPFAMiSSF55785. SSF55785. 2 hits.
    SSF55874. SSF55874. 1 hit.
    TIGRFAMsiTIGR02938. nifL_nitrog. 1 hit.
    TIGR00229. sensory_box. 1 hit.
    PROSITEiPS50109. HIS_KIN. 1 hit.
    PS50113. PAC. 1 hit.
    PS50112. PAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30663-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPANPTLSN EPQAPHAESD ELLPEIFRQT VEHAPIAISI TDLKANILYA    50
    NRAFRTITGY GSEEVLGKNE SILSNGTTPR LVYQALWGRL AQKKPWSGVL 100
    VNRRKDKTLY LAELTVAPVL NEAGETIYYL GMHRDTSELH ELEQRVNNQR 150
    LMIEAVVNAA PAAMVVLDRQ HRVMLSNPSF CRLARDLVED GSSESLVALL 200
    RENLAAPFET LENQGSAFSG KEISFDLGGR SPRWLSCHGR AIHIENEQAH 250
    VFFAPTEERY LLLTINDISE LRQKQQDSRL NALKALMAEE ELLEGMRETF 300
    NAAIHRLQGP VNLISAAMRM LERRLGDKAG NDPVLSAMRE ASTAGMEALE 350
    NLSGSIPVRM AESKMPVNLN QLIREVITLC TDQLLAQGIV VDWQPALRLP 400
    WVMGGESSQR SMIKHLVDNA IESMSQNQVS RRELFISTRV ENHLVRMEIT 450
    DSGPGIPPDL VLKVFEPFFS TKPPHRVGRG MGLPVVQEIV AKHAGMVHVD 500
    TDYREGCRIV VELPFSAST 519
    Length:519
    Mass (Da):57,827
    Last modified:February 1, 1996 - v2
    Checksum:iA40B1E76BD3723CB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64832 Genomic DNA. Translation: CAA46044.1.
    Y00554 Genomic DNA. Translation: CAA68632.1.
    PIRiS19883.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64832 Genomic DNA. Translation: CAA46044.1 .
    Y00554 Genomic DNA. Translation: CAA68632.1 .
    PIRi S19883.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2GJ3 X-ray 1.04 A/B 21-140 [» ]
    ProteinModelPortali P30663.
    SMRi P30663. Positions 22-140.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P30663. 1 interaction.
    MINTi MINT-107527.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P30663.

    Family and domain databases

    Gene3Di 3.30.565.10. 1 hit.
    InterProi IPR003594. HATPase_ATP-bd.
    IPR014285. N_fixation_neg-reg_NifL.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR000700. PAS-assoc_C.
    IPR013767. PAS_fold.
    IPR004358. Sig_transdc_His_kin-like_C.
    IPR005467. Sig_transdc_His_kinase_core.
    [Graphical view ]
    Pfami PF02518. HATPase_c. 1 hit.
    PF00989. PAS. 2 hits.
    [Graphical view ]
    PRINTSi PR00344. BCTRLSENSOR.
    SMARTi SM00387. HATPase_c. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF55785. SSF55785. 2 hits.
    SSF55874. SSF55874. 1 hit.
    TIGRFAMsi TIGR02938. nifL_nitrog. 1 hit.
    TIGR00229. sensory_box. 1 hit.
    PROSITEi PS50109. HIS_KIN. 1 hit.
    PS50113. PAC. 1 hit.
    PS50112. PAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and molecular analysis of the nifL gene of Azotobacter vinelandii."
      Blanco G., Drummond M., Woodley P., Kennedy C.
      Mol. Microbiol. 9:869-879(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.
    2. "Nucleotide sequence and mutagenesis of the nifA gene from Azotobacter vinelandii."
      Bennet L.T., Cannon F., Dean D.R.
      Mol. Microbiol. 2:315-321(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 417-519.
      Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.
    3. "Electron donation to the flavoprotein NifL, a redox-sensing transcriptional regulator."
      Macheroux P., Hill S., Austin S., Eydmann T., Jones T., Kim S.-O., Poole R., Dixon R.
      Biochem. J. 332:413-419(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. "Azotobacter vinelandii NIFL is a flavoprotein that modulates transcriptional activation of nitrogen-fixation genes via a redox-sensitive switch."
      Hill S., Austin S., Eydmann T., Jones T., Dixon R.
      Proc. Natl. Acad. Sci. U.S.A. 93:2143-2148(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FAD AND ADP-BINDING.
    5. "The redox- and fixed nitrogen-responsive regulatory protein NIFL from Azotobacter vinelandii comprises discrete flavin and nucleotide-binding domains."
      Soederbaeck E., Reyes-Ramirez F., Eydmann T., Austin S., Hill S., Dixon R.
      Mol. Microbiol. 28:179-192(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FAD AND ADP-BINDING.
    6. "Crystallization and preliminary crystallographic data of the PAS domain of the NifL protein from Azotobacter vinelandii."
      Hefti M., Hendle J., Enroth C., Vervoort J., Tucker P.A.
      Acta Crystallogr. D 57:1895-1896(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION.

    Entry informationi

    Entry nameiNIFL_AZOVI
    AccessioniPrimary (citable) accession number: P30663
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3