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P30663

- NIFL_AZOVI

UniProt

P30663 - NIFL_AZOVI

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Protein
Nitrogen fixation regulatory protein
Gene
nifL
Organism
Azotobacter vinelandii
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Required for the inhibition of NifA activity in response to oxygen and low level of fixed nitrogen.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

FAD.

Redox potential

E0 is -226 mV.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. flavin adenine dinucleotide binding Source: InterPro
  3. phosphorelay sensor kinase activity Source: InterPro

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: CACAO
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nitrogen fixation, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, FAD, Flavoprotein, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen fixation regulatory protein (EC:2.7.13.3)
Gene namesi
Name:nifL
OrganismiAzotobacter vinelandii
Taxonomic identifieri354 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Nitrogen fixation regulatory protein
PRO_0000074815Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei305 – 3051Phosphohistidine; by autocatalysis By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

IntActiP30663. 1 interaction.
MINTiMINT-107527.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi24 – 3310
Beta strandi35 – 417
Beta strandi46 – 505
Helixi52 – 587
Helixi64 – 674
Helixi70 – 734
Helixi80 – 9112
Beta strandi96 – 1038
Beta strandi109 – 12012
Beta strandi126 – 13510

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GJ3X-ray1.04A/B21-140[»]
ProteinModelPortaliP30663.
SMRiP30663. Positions 22-140.

Miscellaneous databases

EvolutionaryTraceiP30663.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 9371PAS 1
Add
BLAST
Domaini94 – 14855PAC
Add
BLAST
Domaini151 – 21767PAS 2
Add
BLAST
Domaini302 – 517216Histidine kinase
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_ATP-bd.
IPR014285. N_fixation_neg-reg_NifL.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00989. PAS. 2 hits.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 2 hits.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR02938. nifL_nitrog. 1 hit.
TIGR00229. sensory_box. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30663-1 [UniParc]FASTAAdd to Basket

« Hide

MTPANPTLSN EPQAPHAESD ELLPEIFRQT VEHAPIAISI TDLKANILYA    50
NRAFRTITGY GSEEVLGKNE SILSNGTTPR LVYQALWGRL AQKKPWSGVL 100
VNRRKDKTLY LAELTVAPVL NEAGETIYYL GMHRDTSELH ELEQRVNNQR 150
LMIEAVVNAA PAAMVVLDRQ HRVMLSNPSF CRLARDLVED GSSESLVALL 200
RENLAAPFET LENQGSAFSG KEISFDLGGR SPRWLSCHGR AIHIENEQAH 250
VFFAPTEERY LLLTINDISE LRQKQQDSRL NALKALMAEE ELLEGMRETF 300
NAAIHRLQGP VNLISAAMRM LERRLGDKAG NDPVLSAMRE ASTAGMEALE 350
NLSGSIPVRM AESKMPVNLN QLIREVITLC TDQLLAQGIV VDWQPALRLP 400
WVMGGESSQR SMIKHLVDNA IESMSQNQVS RRELFISTRV ENHLVRMEIT 450
DSGPGIPPDL VLKVFEPFFS TKPPHRVGRG MGLPVVQEIV AKHAGMVHVD 500
TDYREGCRIV VELPFSAST 519
Length:519
Mass (Da):57,827
Last modified:February 1, 1996 - v2
Checksum:iA40B1E76BD3723CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64832 Genomic DNA. Translation: CAA46044.1.
Y00554 Genomic DNA. Translation: CAA68632.1.
PIRiS19883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64832 Genomic DNA. Translation: CAA46044.1 .
Y00554 Genomic DNA. Translation: CAA68632.1 .
PIRi S19883.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2GJ3 X-ray 1.04 A/B 21-140 [» ]
ProteinModelPortali P30663.
SMRi P30663. Positions 22-140.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P30663. 1 interaction.
MINTi MINT-107527.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P30663.

Family and domain databases

Gene3Di 3.30.565.10. 1 hit.
InterProi IPR003594. HATPase_ATP-bd.
IPR014285. N_fixation_neg-reg_NifL.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
[Graphical view ]
Pfami PF02518. HATPase_c. 1 hit.
PF00989. PAS. 2 hits.
[Graphical view ]
PRINTSi PR00344. BCTRLSENSOR.
SMARTi SM00387. HATPase_c. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view ]
SUPFAMi SSF55785. SSF55785. 2 hits.
SSF55874. SSF55874. 1 hit.
TIGRFAMsi TIGR02938. nifL_nitrog. 1 hit.
TIGR00229. sensory_box. 1 hit.
PROSITEi PS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence and molecular analysis of the nifL gene of Azotobacter vinelandii."
    Blanco G., Drummond M., Woodley P., Kennedy C.
    Mol. Microbiol. 9:869-879(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.
  2. "Nucleotide sequence and mutagenesis of the nifA gene from Azotobacter vinelandii."
    Bennet L.T., Cannon F., Dean D.R.
    Mol. Microbiol. 2:315-321(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 417-519.
    Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.
  3. "Electron donation to the flavoprotein NifL, a redox-sensing transcriptional regulator."
    Macheroux P., Hill S., Austin S., Eydmann T., Jones T., Kim S.-O., Poole R., Dixon R.
    Biochem. J. 332:413-419(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Azotobacter vinelandii NIFL is a flavoprotein that modulates transcriptional activation of nitrogen-fixation genes via a redox-sensitive switch."
    Hill S., Austin S., Eydmann T., Jones T., Dixon R.
    Proc. Natl. Acad. Sci. U.S.A. 93:2143-2148(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FAD AND ADP-BINDING.
  5. "The redox- and fixed nitrogen-responsive regulatory protein NIFL from Azotobacter vinelandii comprises discrete flavin and nucleotide-binding domains."
    Soederbaeck E., Reyes-Ramirez F., Eydmann T., Austin S., Hill S., Dixon R.
    Mol. Microbiol. 28:179-192(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FAD AND ADP-BINDING.
  6. "Crystallization and preliminary crystallographic data of the PAS domain of the NifL protein from Azotobacter vinelandii."
    Hefti M., Hendle J., Enroth C., Vervoort J., Tucker P.A.
    Acta Crystallogr. D 57:1895-1896(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.

Entry informationi

Entry nameiNIFL_AZOVI
AccessioniPrimary (citable) accession number: P30663
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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