Chromobox protein homolog 2 - Mus musculus (Mouse)

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P30658 (CBX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 113. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chromobox protein homolog 2

Alternative name(s):
M33
Modifier 3 protein

Gene names

Name:	Cbx2
Synonyms:	M33

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Subunit structure	Component of a PRC1-like complex By similarity. Interacts with RING1. Ref.2
Subcellular location	Nucleus speckle Ref.2.
Developmental stage	Expressed in most embryonic tissues except the heart from 8.5 to 11.5 dpc. Expressed in central nervous system (CNS, ventricular zone and spinal cord), peripheral nervous system (PNS, sensory cranial and spinal ganglia), olfactory and tongue epithelia, lung, gastrointestinal duct and urogenital system at 13.5 dpc. Expressed in CNS, thymus, various epithelial cell types including the olfactory, tooth and tongue epithelia at 15.5 dpc. Ref.2
Sequence similarities	Contains 1 A.T hook DNA-binding domain. Contains 1 chromo domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Chromatin regulator Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW development of primary sexual characteristics Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.2 PubMed 9367786. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	PRC1 complex Inferred from sequence or structural similarity. Source: UniProtKB heterochromatin Inferred from direct assay PubMed 16537902. Source: UniProtKB nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW chromatin binding Inferred from direct assay PubMed 16687444. Source: MGI methylated histone residue binding Inferred from direct assay PubMed 16537902. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Rybp	Q8CCI5	3	EBI-360174,EBI-929290

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 519

519

Chromobox protein homolog 2

PRO_0000080202

Regions

Domain

12 – 70

Chromo

DNA binding

75 – 87

A.T hook

Motif

164 – 169

Nuclear localization signal Potential

Compositional bias

102 – 120

Ser-rich

Compositional bias

121 – 130

Asp/Glu-rich (acidic)

Experimental info

Sequence conflict

M → EF in CAA73723. Ref.2

Sequence conflict

V → A in CAA44398. Ref.1

Sequence conflict

107

S → G in CAA73723. Ref.2

Sequence conflict

133

R → G in CAA73723. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P30658 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: F89EF3CEF14ACA72
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FASTA

519

54,918

        10         20         30         40         50         60 
MEELSSVGEQ VFAAECILSK RLRKGKLEYL VKWRGWSSKH NSWEPEENIL DPRLLLAFQK 

        70         80         90        100        110        120 
KEHEKEVQNR KRGKRPRGRP RKHTVTSSCS RRSKLKEPDA PSKSKSSSSS SSSTSSSSSS 

       130        140        150        160        170        180 
DEEEDDSDLD SKRGPRGRET HPVPQKKAQI LVAKPELKDP IRKKRGRKPL PPEQKAARRP 

       190        200        210        220        230        240 
VSLAKVLKTT RKDLGTSAAK LPPPLSAPVA GLAALKAHTK EACGGPSTMA TPENLASLMK 

       250        260        270        280        290        300 
GMAGSPSRGG IWQSSIVHYM NRMSQSQVQA ASRLALKAQA TNKCGLGLDL KVRTQKGGEL 

       310        320        330        340        350        360 
GGSPAGGKVP KAPGGGAAEQ QRGNHSGSPG AQLAPTQELS LQVLDLQSVK NGVPGVGLLA 

       370        380        390        400        410        420 
RHAPAKAIPA TNPATGKGPG SGPTGANMTN APTDNNKGEK LTCKATALPA PSVKRDTVKS 

       430        440        450        460        470        480 
VAASGGQEGH TAPGEGRKPP ALSELSTGEE NSSSDSDPDS TSLPSAAQNL SVAIQTSQDW 

       490        500        510 
KPTRSLIEHV FVTDVTANLI TVTVKESPTS VGFFNLRHY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mouse has a Polycomb-like chromobox gene." Pearce J.J.H., Singh P.B., Gaunt S.J. Development 114:921-930(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Embryo.
[2]	"Ring1A is a transcriptional repressor that interacts with the Polycomb-M33 protein and is expressed at rhombomere boundaries in the mouse hindbrain." Schoorlemmer J., Marcos-Gutierrez C., Were F., Martinez R., Garcia E., Satijn D.P.E., Otte A.P., Vidal M. EMBO J. 16:5930-5942(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RING1, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION. Tissue: Embryo.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62537 mRNA. Translation: CAA44398.1. Y13274 mRNA. Translation: CAA73723.1. AL662835 Genomic DNA. Translation: CAM24931.1. CH466558 Genomic DNA. Translation: EDL34680.1. BC035199 mRNA. Translation: AAH35199.1.
IPI	IPI00132317.
PIR	S23796.
RefSeq	NP_031649.2. NM_007623.2.
UniGene	Mm.14547.
3D structure databases
ProteinModelPortal	P30658.
SMR	P30658. Positions 9-60.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-690N.
IntAct	P30658. 17 interactions.
PTM databases
PhosphoSite	P30658.
Proteomic databases
PaxDb	P30658.
PRIDE	P30658.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026662; ENSMUSP00000026662; ENSMUSG00000025577.
GeneID	12416.
KEGG	mmu:12416.
Organism-specific databases
CTD	84733.
MGI	MGI:88289. Cbx2.
Phylogenomic databases
eggNOG	NOG117427.
GeneTree	ENSGT00530000063056.
HOGENOM	HOG000231217.
HOVERGEN	HBG005256.
InParanoid	A2ABG2.
KO	K11451.
OMA	ASLMKGM.
OrthoDB	EOG4Q84ZX.
Gene expression databases
ArrayExpress	P30658.
Bgee	P30658.
CleanEx	MM_CBX2.
Genevestigator	P30658.
GermOnline	ENSMUSG00000025577. Mus musculus.
Family and domain databases
InterPro	IPR017956. AT_hook_DNA-bd_motif. IPR023780. Chromo_domain. IPR000953. Chromo_domain/shadow. IPR016197. Chromodomain-like. IPR023779. Chromodomain_CS. [Graphical view]
Pfam	PF00385. Chromo. 1 hit. [Graphical view]
SMART	SM00384. AT_hook. 1 hit. SM00298. CHROMO. 1 hit. [Graphical view]
SUPFAM	SSF54160. Chromodomain-like. 1 hit.
PROSITE	PS00598. CHROMO_1. 1 hit. PS50013. CHROMO_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281204.
SOURCE	Search...

Entry information

Entry name

CBX2_MOUSE

Accession

Primary (citable) accession number: P30658
Secondary accession number(s): A2ABG2, O35731, Q8CIA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents