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P30658 (CBX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chromobox protein homolog 2
Alternative name(s):
M33
Modifier 3 protein
Gene names
Name:Cbx2
Synonyms:M33
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length519 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Subunit structure

Component of a PRC1-like complex By similarity. Interacts with RING1. Ref.2

Subcellular location

Nucleus speckle Ref.2.

Developmental stage

Expressed in most embryonic tissues except the heart from 8.5 to 11.5 dpc. Expressed in central nervous system (CNS, ventricular zone and spinal cord), peripheral nervous system (PNS, sensory cranial and spinal ganglia), olfactory and tongue epithelia, lung, gastrointestinal duct and urogenital system at 13.5 dpc. Expressed in CNS, thymus, various epithelial cell types including the olfactory, tooth and tongue epithelia at 15.5 dpc. Ref.2

Sequence similarities

Contains 1 A.T hook DNA-binding domain.

Contains 1 chromo domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RybpQ8CCI53EBI-360174,EBI-929290

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 519519Chromobox protein homolog 2
PRO_0000080202

Regions

Domain12 – 7059Chromo
DNA binding75 – 8713A.T hook
Motif164 – 1696Nuclear localization signal Potential
Compositional bias102 – 12019Ser-rich
Compositional bias121 – 13010Asp/Glu-rich (acidic)

Experimental info

Sequence conflict11M → EF in CAA73723. Ref.2
Sequence conflict851V → A in CAA44398. Ref.1
Sequence conflict1071S → G in CAA73723. Ref.2
Sequence conflict1331R → G in CAA73723. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P30658 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: F89EF3CEF14ACA72

FASTA51954,918
        10         20         30         40         50         60 
MEELSSVGEQ VFAAECILSK RLRKGKLEYL VKWRGWSSKH NSWEPEENIL DPRLLLAFQK 

        70         80         90        100        110        120 
KEHEKEVQNR KRGKRPRGRP RKHTVTSSCS RRSKLKEPDA PSKSKSSSSS SSSTSSSSSS 

       130        140        150        160        170        180 
DEEEDDSDLD SKRGPRGRET HPVPQKKAQI LVAKPELKDP IRKKRGRKPL PPEQKAARRP 

       190        200        210        220        230        240 
VSLAKVLKTT RKDLGTSAAK LPPPLSAPVA GLAALKAHTK EACGGPSTMA TPENLASLMK 

       250        260        270        280        290        300 
GMAGSPSRGG IWQSSIVHYM NRMSQSQVQA ASRLALKAQA TNKCGLGLDL KVRTQKGGEL 

       310        320        330        340        350        360 
GGSPAGGKVP KAPGGGAAEQ QRGNHSGSPG AQLAPTQELS LQVLDLQSVK NGVPGVGLLA 

       370        380        390        400        410        420 
RHAPAKAIPA TNPATGKGPG SGPTGANMTN APTDNNKGEK LTCKATALPA PSVKRDTVKS 

       430        440        450        460        470        480 
VAASGGQEGH TAPGEGRKPP ALSELSTGEE NSSSDSDPDS TSLPSAAQNL SVAIQTSQDW 

       490        500        510 
KPTRSLIEHV FVTDVTANLI TVTVKESPTS VGFFNLRHY 

« Hide

References

« Hide 'large scale' references
[1]"The mouse has a Polycomb-like chromobox gene."
Pearce J.J.H., Singh P.B., Gaunt S.J.
Development 114:921-930(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Embryo.
[2]"Ring1A is a transcriptional repressor that interacts with the Polycomb-M33 protein and is expressed at rhombomere boundaries in the mouse hindbrain."
Schoorlemmer J., Marcos-Gutierrez C., Were F., Martinez R., Garcia E., Satijn D.P.E., Otte A.P., Vidal M.
EMBO J. 16:5930-5942(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RING1, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
Tissue: Embryo.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62537 mRNA. Translation: CAA44398.1.
Y13274 mRNA. Translation: CAA73723.1.
AL662835 Genomic DNA. Translation: CAM24931.1.
CH466558 Genomic DNA. Translation: EDL34680.1.
BC035199 mRNA. Translation: AAH35199.1.
PIRS23796.
RefSeqNP_031649.2. NM_007623.2.
UniGeneMm.14547.

3D structure databases

ProteinModelPortalP30658.
SMRP30658. Positions 9-60, 489-517.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198535. 19 interactions.
DIPDIP-690N.
IntActP30658. 15 interactions.

PTM databases

PhosphoSiteP30658.

Proteomic databases

PaxDbP30658.
PRIDEP30658.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026662; ENSMUSP00000026662; ENSMUSG00000025577.
GeneID12416.
KEGGmmu:12416.
UCSCuc007mpt.1. mouse.

Organism-specific databases

CTD84733.
MGIMGI:88289. Cbx2.

Phylogenomic databases

eggNOGNOG117427.
GeneTreeENSGT00530000063056.
HOGENOMHOG000231217.
HOVERGENHBG005256.
InParanoidA2ABG2.
KOK11451.
OMAASLMKGM.
OrthoDBEOG7S7SF4.
TreeFamTF106456.

Gene expression databases

ArrayExpressP30658.
BgeeP30658.
CleanExMM_CBX2.
GenevestigatorP30658.

Family and domain databases

InterProIPR017956. AT_hook_DNA-bd_motif.
IPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
[Graphical view]
PfamPF00385. Chromo. 1 hit.
[Graphical view]
SMARTSM00384. AT_hook. 1 hit.
SM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMSSF54160. SSF54160. 1 hit.
PROSITEPS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281204.
PROP30658.
SOURCESearch...

Entry information

Entry nameCBX2_MOUSE
AccessionPrimary (citable) accession number: P30658
Secondary accession number(s): A2ABG2, O35731, Q8CIA0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot