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Protein

Chromobox protein homolog 2

Gene

Cbx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi75 – 8713A.T hookAdd
BLAST

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: UniProtKB-KW
  • methylated histone binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_305463. Oxidative Stress Induced Senescence.
REACT_360954. SUMOylation of DNA damage response and repair proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromobox protein homolog 2
Alternative name(s):
M33
Modifier 3 protein
Gene namesi
Name:Cbx2
Synonyms:M33
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:88289. Cbx2.

Subcellular locationi

GO - Cellular componenti

  • euchromatin Source: MGI
  • heterochromatin Source: UniProtKB
  • nuclear speck Source: UniProtKB-SubCell
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • PcG protein complex Source: MGI
  • PRC1 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Chromobox protein homolog 2PRO_0000080202Add
BLAST

Proteomic databases

MaxQBiP30658.
PaxDbiP30658.
PRIDEiP30658.

PTM databases

PhosphoSiteiP30658.

Expressioni

Developmental stagei

Expressed in most embryonic tissues except the heart from 8.5 to 11.5 dpc. Expressed in central nervous system (CNS, ventricular zone and spinal cord), peripheral nervous system (PNS, sensory cranial and spinal ganglia), olfactory and tongue epithelia, lung, gastrointestinal duct and urogenital system at 13.5 dpc. Expressed in CNS, thymus, various epithelial cell types including the olfactory, tooth and tongue epithelia at 15.5 dpc.1 Publication

Gene expression databases

BgeeiP30658.
CleanExiMM_CBX2.
ExpressionAtlasiP30658. baseline and differential.
GenevisibleiP30658. MM.

Interactioni

Subunit structurei

Component of a PRC1-like complex (By similarity). Interacts with RING1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RybpQ8CCI53EBI-360174,EBI-929290

Protein-protein interaction databases

BioGridi198535. 19 interactions.
DIPiDIP-690N.
IntActiP30658. 15 interactions.
STRINGi10090.ENSMUSP00000026662.

Structurei

3D structure databases

ProteinModelPortaliP30658.
SMRiP30658. Positions 9-60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 7059ChromoPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi164 – 1696Nuclear localization signalSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi102 – 12019Ser-richAdd
BLAST
Compositional biasi121 – 13010Asp/Glu-rich (acidic)

Sequence similaritiesi

Contains 1 A.T hook DNA-binding domain.Curated
Contains 1 chromo domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG117427.
GeneTreeiENSGT00530000063056.
HOGENOMiHOG000231217.
HOVERGENiHBG005256.
InParanoidiP30658.
KOiK11451.
OMAiSRHNSWE.
OrthoDBiEOG7S7SF4.
TreeFamiTF106456.

Family and domain databases

InterProiIPR017956. AT_hook_DNA-bd_motif.
IPR000953. Chromo/shadow_dom.
IPR023780. Chromo_domain.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
[Graphical view]
PfamiPF00385. Chromo. 1 hit.
[Graphical view]
SMARTiSM00384. AT_hook. 1 hit.
SM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 1 hit.
PROSITEiPS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30658-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELSSVGEQ VFAAECILSK RLRKGKLEYL VKWRGWSSKH NSWEPEENIL
60 70 80 90 100
DPRLLLAFQK KEHEKEVQNR KRGKRPRGRP RKHTVTSSCS RRSKLKEPDA
110 120 130 140 150
PSKSKSSSSS SSSTSSSSSS DEEEDDSDLD SKRGPRGRET HPVPQKKAQI
160 170 180 190 200
LVAKPELKDP IRKKRGRKPL PPEQKAARRP VSLAKVLKTT RKDLGTSAAK
210 220 230 240 250
LPPPLSAPVA GLAALKAHTK EACGGPSTMA TPENLASLMK GMAGSPSRGG
260 270 280 290 300
IWQSSIVHYM NRMSQSQVQA ASRLALKAQA TNKCGLGLDL KVRTQKGGEL
310 320 330 340 350
GGSPAGGKVP KAPGGGAAEQ QRGNHSGSPG AQLAPTQELS LQVLDLQSVK
360 370 380 390 400
NGVPGVGLLA RHAPAKAIPA TNPATGKGPG SGPTGANMTN APTDNNKGEK
410 420 430 440 450
LTCKATALPA PSVKRDTVKS VAASGGQEGH TAPGEGRKPP ALSELSTGEE
460 470 480 490 500
NSSSDSDPDS TSLPSAAQNL SVAIQTSQDW KPTRSLIEHV FVTDVTANLI
510
TVTVKESPTS VGFFNLRHY
Length:519
Mass (Da):54,918
Last modified:July 27, 2011 - v2
Checksum:iF89EF3CEF14ACA72
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11M → EF in CAA73723 (PubMed:9312051).Curated
Sequence conflicti85 – 851V → A in CAA44398 (PubMed:1352241).Curated
Sequence conflicti107 – 1071S → G in CAA73723 (PubMed:9312051).Curated
Sequence conflicti133 – 1331R → G in CAA73723 (PubMed:9312051).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62537 mRNA. Translation: CAA44398.1.
Y13274 mRNA. Translation: CAA73723.1.
AL662835 Genomic DNA. Translation: CAM24931.1.
CH466558 Genomic DNA. Translation: EDL34680.1.
BC035199 mRNA. Translation: AAH35199.1.
CCDSiCCDS25708.1.
PIRiS23796.
RefSeqiNP_031649.2. NM_007623.2.
UniGeneiMm.14547.

Genome annotation databases

EnsembliENSMUST00000026662; ENSMUSP00000026662; ENSMUSG00000025577.
GeneIDi12416.
KEGGimmu:12416.
UCSCiuc007mpt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62537 mRNA. Translation: CAA44398.1.
Y13274 mRNA. Translation: CAA73723.1.
AL662835 Genomic DNA. Translation: CAM24931.1.
CH466558 Genomic DNA. Translation: EDL34680.1.
BC035199 mRNA. Translation: AAH35199.1.
CCDSiCCDS25708.1.
PIRiS23796.
RefSeqiNP_031649.2. NM_007623.2.
UniGeneiMm.14547.

3D structure databases

ProteinModelPortaliP30658.
SMRiP30658. Positions 9-60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198535. 19 interactions.
DIPiDIP-690N.
IntActiP30658. 15 interactions.
STRINGi10090.ENSMUSP00000026662.

PTM databases

PhosphoSiteiP30658.

Proteomic databases

MaxQBiP30658.
PaxDbiP30658.
PRIDEiP30658.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026662; ENSMUSP00000026662; ENSMUSG00000025577.
GeneIDi12416.
KEGGimmu:12416.
UCSCiuc007mpt.1. mouse.

Organism-specific databases

CTDi84733.
MGIiMGI:88289. Cbx2.

Phylogenomic databases

eggNOGiNOG117427.
GeneTreeiENSGT00530000063056.
HOGENOMiHOG000231217.
HOVERGENiHBG005256.
InParanoidiP30658.
KOiK11451.
OMAiSRHNSWE.
OrthoDBiEOG7S7SF4.
TreeFamiTF106456.

Enzyme and pathway databases

ReactomeiREACT_305463. Oxidative Stress Induced Senescence.
REACT_360954. SUMOylation of DNA damage response and repair proteins.

Miscellaneous databases

ChiTaRSiCbx2. mouse.
NextBioi281204.
PROiP30658.
SOURCEiSearch...

Gene expression databases

BgeeiP30658.
CleanExiMM_CBX2.
ExpressionAtlasiP30658. baseline and differential.
GenevisibleiP30658. MM.

Family and domain databases

InterProiIPR017956. AT_hook_DNA-bd_motif.
IPR000953. Chromo/shadow_dom.
IPR023780. Chromo_domain.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
[Graphical view]
PfamiPF00385. Chromo. 1 hit.
[Graphical view]
SMARTiSM00384. AT_hook. 1 hit.
SM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 1 hit.
PROSITEiPS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse has a Polycomb-like chromobox gene."
    Pearce J.J.H., Singh P.B., Gaunt S.J.
    Development 114:921-930(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Embryo.
  2. "Ring1A is a transcriptional repressor that interacts with the Polycomb-M33 protein and is expressed at rhombomere boundaries in the mouse hindbrain."
    Schoorlemmer J., Marcos-Gutierrez C., Were F., Martinez R., Garcia E., Satijn D.P.E., Otte A.P., Vidal M.
    EMBO J. 16:5930-5942(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RING1, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
    Tissue: Embryo.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.

Entry informationi

Entry nameiCBX2_MOUSE
AccessioniPrimary (citable) accession number: P30658
Secondary accession number(s): A2ABG2, O35731, Q8CIA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.