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P30658

- CBX2_MOUSE

UniProt

P30658 - CBX2_MOUSE

Protein

Chromobox protein homolog 2

Gene

Cbx2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi75 – 8713A.T hookAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: UniProtKB-KW
    3. methylated histone binding Source: UniProtKB
    4. protein binding Source: IntAct

    GO - Biological processi

    1. chromatin modification Source: UniProtKB-KW
    2. development of primary sexual characteristics Source: Ensembl
    3. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_188970. Oxidative Stress Induced Senescence.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chromobox protein homolog 2
    Alternative name(s):
    M33
    Modifier 3 protein
    Gene namesi
    Name:Cbx2
    Synonyms:M33
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:88289. Cbx2.

    Subcellular locationi

    Nucleus speckle 1 Publication

    GO - Cellular componenti

    1. euchromatin Source: MGI
    2. heterochromatin Source: UniProtKB
    3. nuclear speck Source: UniProtKB-SubCell
    4. nucleus Source: UniProtKB
    5. PcG protein complex Source: MGI
    6. PRC1 complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 519519Chromobox protein homolog 2PRO_0000080202Add
    BLAST

    Proteomic databases

    MaxQBiP30658.
    PaxDbiP30658.
    PRIDEiP30658.

    PTM databases

    PhosphoSiteiP30658.

    Expressioni

    Developmental stagei

    Expressed in most embryonic tissues except the heart from 8.5 to 11.5 dpc. Expressed in central nervous system (CNS, ventricular zone and spinal cord), peripheral nervous system (PNS, sensory cranial and spinal ganglia), olfactory and tongue epithelia, lung, gastrointestinal duct and urogenital system at 13.5 dpc. Expressed in CNS, thymus, various epithelial cell types including the olfactory, tooth and tongue epithelia at 15.5 dpc.1 Publication

    Gene expression databases

    ArrayExpressiP30658.
    BgeeiP30658.
    CleanExiMM_CBX2.
    GenevestigatoriP30658.

    Interactioni

    Subunit structurei

    Component of a PRC1-like complex By similarity. Interacts with RING1.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RybpQ8CCI53EBI-360174,EBI-929290

    Protein-protein interaction databases

    BioGridi198535. 19 interactions.
    DIPiDIP-690N.
    IntActiP30658. 15 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP30658.
    SMRiP30658. Positions 9-60.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 7059ChromoPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi164 – 1696Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi102 – 12019Ser-richAdd
    BLAST
    Compositional biasi121 – 13010Asp/Glu-rich (acidic)

    Sequence similaritiesi

    Contains 1 A.T hook DNA-binding domain.Curated
    Contains 1 chromo domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG117427.
    GeneTreeiENSGT00530000063056.
    HOGENOMiHOG000231217.
    HOVERGENiHBG005256.
    InParanoidiA2ABG2.
    KOiK11451.
    OMAiASLMKGM.
    OrthoDBiEOG7S7SF4.
    TreeFamiTF106456.

    Family and domain databases

    InterProiIPR017956. AT_hook_DNA-bd_motif.
    IPR023780. Chromo_domain.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR023779. Chromodomain_CS.
    [Graphical view]
    PfamiPF00385. Chromo. 1 hit.
    [Graphical view]
    SMARTiSM00384. AT_hook. 1 hit.
    SM00298. CHROMO. 1 hit.
    [Graphical view]
    SUPFAMiSSF54160. SSF54160. 1 hit.
    PROSITEiPS00598. CHROMO_1. 1 hit.
    PS50013. CHROMO_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30658-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEELSSVGEQ VFAAECILSK RLRKGKLEYL VKWRGWSSKH NSWEPEENIL    50
    DPRLLLAFQK KEHEKEVQNR KRGKRPRGRP RKHTVTSSCS RRSKLKEPDA 100
    PSKSKSSSSS SSSTSSSSSS DEEEDDSDLD SKRGPRGRET HPVPQKKAQI 150
    LVAKPELKDP IRKKRGRKPL PPEQKAARRP VSLAKVLKTT RKDLGTSAAK 200
    LPPPLSAPVA GLAALKAHTK EACGGPSTMA TPENLASLMK GMAGSPSRGG 250
    IWQSSIVHYM NRMSQSQVQA ASRLALKAQA TNKCGLGLDL KVRTQKGGEL 300
    GGSPAGGKVP KAPGGGAAEQ QRGNHSGSPG AQLAPTQELS LQVLDLQSVK 350
    NGVPGVGLLA RHAPAKAIPA TNPATGKGPG SGPTGANMTN APTDNNKGEK 400
    LTCKATALPA PSVKRDTVKS VAASGGQEGH TAPGEGRKPP ALSELSTGEE 450
    NSSSDSDPDS TSLPSAAQNL SVAIQTSQDW KPTRSLIEHV FVTDVTANLI 500
    TVTVKESPTS VGFFNLRHY 519
    Length:519
    Mass (Da):54,918
    Last modified:July 27, 2011 - v2
    Checksum:iF89EF3CEF14ACA72
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 11M → EF in CAA73723. (PubMed:9312051)Curated
    Sequence conflicti85 – 851V → A in CAA44398. (PubMed:1352241)Curated
    Sequence conflicti107 – 1071S → G in CAA73723. (PubMed:9312051)Curated
    Sequence conflicti133 – 1331R → G in CAA73723. (PubMed:9312051)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62537 mRNA. Translation: CAA44398.1.
    Y13274 mRNA. Translation: CAA73723.1.
    AL662835 Genomic DNA. Translation: CAM24931.1.
    CH466558 Genomic DNA. Translation: EDL34680.1.
    BC035199 mRNA. Translation: AAH35199.1.
    CCDSiCCDS25708.1.
    PIRiS23796.
    RefSeqiNP_031649.2. NM_007623.2.
    UniGeneiMm.14547.

    Genome annotation databases

    EnsembliENSMUST00000026662; ENSMUSP00000026662; ENSMUSG00000025577.
    GeneIDi12416.
    KEGGimmu:12416.
    UCSCiuc007mpt.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62537 mRNA. Translation: CAA44398.1 .
    Y13274 mRNA. Translation: CAA73723.1 .
    AL662835 Genomic DNA. Translation: CAM24931.1 .
    CH466558 Genomic DNA. Translation: EDL34680.1 .
    BC035199 mRNA. Translation: AAH35199.1 .
    CCDSi CCDS25708.1.
    PIRi S23796.
    RefSeqi NP_031649.2. NM_007623.2.
    UniGenei Mm.14547.

    3D structure databases

    ProteinModelPortali P30658.
    SMRi P30658. Positions 9-60.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198535. 19 interactions.
    DIPi DIP-690N.
    IntActi P30658. 15 interactions.

    PTM databases

    PhosphoSitei P30658.

    Proteomic databases

    MaxQBi P30658.
    PaxDbi P30658.
    PRIDEi P30658.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026662 ; ENSMUSP00000026662 ; ENSMUSG00000025577 .
    GeneIDi 12416.
    KEGGi mmu:12416.
    UCSCi uc007mpt.1. mouse.

    Organism-specific databases

    CTDi 84733.
    MGIi MGI:88289. Cbx2.

    Phylogenomic databases

    eggNOGi NOG117427.
    GeneTreei ENSGT00530000063056.
    HOGENOMi HOG000231217.
    HOVERGENi HBG005256.
    InParanoidi A2ABG2.
    KOi K11451.
    OMAi ASLMKGM.
    OrthoDBi EOG7S7SF4.
    TreeFami TF106456.

    Enzyme and pathway databases

    Reactomei REACT_188970. Oxidative Stress Induced Senescence.

    Miscellaneous databases

    NextBioi 281204.
    PROi P30658.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P30658.
    Bgeei P30658.
    CleanExi MM_CBX2.
    Genevestigatori P30658.

    Family and domain databases

    InterProi IPR017956. AT_hook_DNA-bd_motif.
    IPR023780. Chromo_domain.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR023779. Chromodomain_CS.
    [Graphical view ]
    Pfami PF00385. Chromo. 1 hit.
    [Graphical view ]
    SMARTi SM00384. AT_hook. 1 hit.
    SM00298. CHROMO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54160. SSF54160. 1 hit.
    PROSITEi PS00598. CHROMO_1. 1 hit.
    PS50013. CHROMO_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The mouse has a Polycomb-like chromobox gene."
      Pearce J.J.H., Singh P.B., Gaunt S.J.
      Development 114:921-930(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Embryo.
    2. "Ring1A is a transcriptional repressor that interacts with the Polycomb-M33 protein and is expressed at rhombomere boundaries in the mouse hindbrain."
      Schoorlemmer J., Marcos-Gutierrez C., Were F., Martinez R., Garcia E., Satijn D.P.E., Otte A.P., Vidal M.
      EMBO J. 16:5930-5942(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RING1, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
      Tissue: Embryo.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.

    Entry informationi

    Entry nameiCBX2_MOUSE
    AccessioniPrimary (citable) accession number: P30658
    Secondary accession number(s): A2ABG2, O35731, Q8CIA0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3