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Protein

Chromobox protein homolog 2

Gene

Cbx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi75 – 87A.T hookAdd BLAST13

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: UniProtKB-KW
  • methylated histone binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-2559580. Oxidative Stress Induced Senescence.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-4551638. SUMOylation of chromatin organization proteins.
R-MMU-4570464. SUMOylation of RNA binding proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromobox protein homolog 2
Alternative name(s):
M33
Modifier 3 protein
Gene namesi
Name:Cbx2
Synonyms:M33
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:88289. Cbx2.

Subcellular locationi

GO - Cellular componenti

  • euchromatin Source: MGI
  • heterochromatin Source: UniProtKB
  • nuclear speck Source: UniProtKB-SubCell
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • PcG protein complex Source: MGI
  • PRC1 complex Source: UniProtKB

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802021 – 519Chromobox protein homolog 2Add BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei248Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei248Omega-N-methylarginine; alternateCombined sources1
Modified residuei303PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP30658.
MaxQBiP30658.
PaxDbiP30658.
PeptideAtlasiP30658.
PRIDEiP30658.

PTM databases

iPTMnetiP30658.
PhosphoSitePlusiP30658.

Expressioni

Developmental stagei

Expressed in most embryonic tissues except the heart from 8.5 to 11.5 dpc. Expressed in central nervous system (CNS, ventricular zone and spinal cord), peripheral nervous system (PNS, sensory cranial and spinal ganglia), olfactory and tongue epithelia, lung, gastrointestinal duct and urogenital system at 13.5 dpc. Expressed in CNS, thymus, various epithelial cell types including the olfactory, tooth and tongue epithelia at 15.5 dpc.1 Publication

Gene expression databases

BgeeiENSMUSG00000025577.
CleanExiMM_CBX2.
GenevisibleiP30658. MM.

Interactioni

Subunit structurei

Component of a PRC1-like complex (By similarity). Interacts with RING1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RybpQ8CCI53EBI-360174,EBI-929290

GO - Molecular functioni

  • methylated histone binding Source: UniProtKB

Protein-protein interaction databases

BioGridi198535. 19 interactors.
DIPiDIP-690N.
IntActiP30658. 15 interactors.
STRINGi10090.ENSMUSP00000026662.

Structurei

3D structure databases

ProteinModelPortaliP30658.
SMRiP30658.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 70ChromoPROSITE-ProRule annotationAdd BLAST59

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi164 – 169Nuclear localization signalSequence analysis6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi102 – 120Ser-richAdd BLAST19
Compositional biasi121 – 130Asp/Glu-rich (acidic)10

Phylogenomic databases

eggNOGiENOG410IPQ5. Eukaryota.
ENOG4111HE0. LUCA.
GeneTreeiENSGT00530000063056.
HOGENOMiHOG000231217.
HOVERGENiHBG005256.
InParanoidiP30658.
KOiK11451.
OMAiKCGLGLD.
OrthoDBiEOG091G08J1.
TreeFamiTF106456.

Family and domain databases

InterProiView protein in InterPro
IPR033773. CBX7_C.
IPR000953. Chromo/chromo_shadow_dom.
IPR023780. Chromo_domain.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
PfamiView protein in Pfam
PF17218. CBX7_C. 1 hit.
PF00385. Chromo. 1 hit.
SMARTiView protein in SMART
SM00298. CHROMO. 1 hit.
SUPFAMiSSF54160. SSF54160. 1 hit.
PROSITEiView protein in PROSITE
PS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P30658-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELSSVGEQ VFAAECILSK RLRKGKLEYL VKWRGWSSKH NSWEPEENIL
60 70 80 90 100
DPRLLLAFQK KEHEKEVQNR KRGKRPRGRP RKHTVTSSCS RRSKLKEPDA
110 120 130 140 150
PSKSKSSSSS SSSTSSSSSS DEEEDDSDLD SKRGPRGRET HPVPQKKAQI
160 170 180 190 200
LVAKPELKDP IRKKRGRKPL PPEQKAARRP VSLAKVLKTT RKDLGTSAAK
210 220 230 240 250
LPPPLSAPVA GLAALKAHTK EACGGPSTMA TPENLASLMK GMAGSPSRGG
260 270 280 290 300
IWQSSIVHYM NRMSQSQVQA ASRLALKAQA TNKCGLGLDL KVRTQKGGEL
310 320 330 340 350
GGSPAGGKVP KAPGGGAAEQ QRGNHSGSPG AQLAPTQELS LQVLDLQSVK
360 370 380 390 400
NGVPGVGLLA RHAPAKAIPA TNPATGKGPG SGPTGANMTN APTDNNKGEK
410 420 430 440 450
LTCKATALPA PSVKRDTVKS VAASGGQEGH TAPGEGRKPP ALSELSTGEE
460 470 480 490 500
NSSSDSDPDS TSLPSAAQNL SVAIQTSQDW KPTRSLIEHV FVTDVTANLI
510
TVTVKESPTS VGFFNLRHY
Length:519
Mass (Da):54,918
Last modified:July 27, 2011 - v2
Checksum:iF89EF3CEF14ACA72
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1M → EF in CAA73723 (PubMed:9312051).Curated1
Sequence conflicti85V → A in CAA44398 (PubMed:1352241).Curated1
Sequence conflicti107S → G in CAA73723 (PubMed:9312051).Curated1
Sequence conflicti133R → G in CAA73723 (PubMed:9312051).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62537 mRNA. Translation: CAA44398.1.
Y13274 mRNA. Translation: CAA73723.1.
AL662835 Genomic DNA. Translation: CAM24931.1.
CH466558 Genomic DNA. Translation: EDL34680.1.
BC035199 mRNA. Translation: AAH35199.1.
CCDSiCCDS25708.1.
PIRiS23796.
RefSeqiNP_031649.2. NM_007623.3.
UniGeneiMm.14547.

Genome annotation databases

EnsembliENSMUST00000026662; ENSMUSP00000026662; ENSMUSG00000025577.
GeneIDi12416.
KEGGimmu:12416.
UCSCiuc007mpt.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCBX2_MOUSE
AccessioniPrimary (citable) accession number: P30658
Secondary accession number(s): A2ABG2, O35731, Q8CIA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 27, 2011
Last modified: August 30, 2017
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot