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P30655

- PSB5_SCHPO

UniProt

P30655 - PSB5_SCHPO

Protein

Probable proteasome subunit beta type-5

Gene

pts1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 3 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei62 – 621NucleophileBy similarity

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. proteasome-mediated ubiquitin-dependent protein catabolic process Source: PomBase
    2. regulation of mitotic cell cycle Source: PomBase

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    ReactomeiREACT_188524. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_188566. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_188568. ER-Phagosome pathway.
    REACT_188587. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_208775. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_215140. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_215320. Orc1 removal from chromatin.
    REACT_218991. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_81558. Regulation of activated PAK-2p34 by proteasome mediated degradation.

    Protein family/group databases

    MEROPSiT01.012.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable proteasome subunit beta type-5 (EC:3.4.25.1)
    Alternative name(s):
    Macropain subunit pts1
    Multicatalytic endopeptidase complex subunit pts1
    Proteasome component pts1
    Gene namesi
    Name:pts1
    ORF Names:SPAC4A8.13c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC4A8.13c.

    Subcellular locationi

    Cytoplasm 1 PublicationPROSITE-ProRule annotation. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. nuclear envelope Source: PomBase
    3. nucleus Source: PomBase
    4. proteasome core complex, beta-subunit complex Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 6161Removed in mature formBy similarityPRO_0000026609Add
    BLAST
    Chaini62 – 272211Probable proteasome subunit beta type-5PRO_0000026610Add
    BLAST

    Keywords - PTMi

    Zymogen

    Proteomic databases

    MaxQBiP30655.
    PaxDbiP30655.

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.By similarity

    Protein-protein interaction databases

    BioGridi280037. 2 interactions.
    MINTiMINT-4688233.
    STRINGi4896.SPAC4A8.13c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP30655.
    SMRiP30655. Positions 62-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    HOGENOMiHOG000091082.
    KOiK02737.
    OMAiTMCAGVT.
    OrthoDBiEOG70W3R5.
    PhylomeDBiP30655.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    PRINTSiPR00141. PROTEASOME.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30655-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSIVSKYTQ STNNDDPKKI IEEEGFTNRF DVVPVPQSSL YLRNLTDETK    50
    NKHCLIKMNH GTTTLAFRYQ HGIVVCVDSR ASAGPLIASQ TVKKVIEINP 100
    YLLGTLAGGA ADCQFWETVL GMECRLHQLR NKELISVSAA SKILSNITYS 150
    YKGYGLSMGT MLAGTGKGGT ALYYIDSDGT RLKGDLFSVG SGSTFAYGVL 200
    DSGYRWDLSK QEALYLAQRS IVAATHRDAY SGGSVNLYHI DENGWVFHGN 250
    FDVDSLIWEA KDNENSFAHI PR 272
    Length:272
    Mass (Da):29,987
    Last modified:July 15, 1998 - v3
    Checksum:i3FC37D02A06B5E61
    GO

    Sequence cautioni

    The sequence CAA58746.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13094 Genomic DNA. Translation: BAA02403.1.
    CU329670 Genomic DNA. Translation: CAB11483.1.
    X83866 Genomic DNA. Translation: CAA58746.1. Different initiation.
    PIRiJS0753.
    T38783.
    RefSeqiNP_593825.1. NM_001019254.2.

    Genome annotation databases

    EnsemblFungiiSPAC4A8.13c.1; SPAC4A8.13c.1:pep; SPAC4A8.13c.
    GeneIDi2543623.
    KEGGispo:SPAC4A8.13c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13094 Genomic DNA. Translation: BAA02403.1 .
    CU329670 Genomic DNA. Translation: CAB11483.1 .
    X83866 Genomic DNA. Translation: CAA58746.1 . Different initiation.
    PIRi JS0753.
    T38783.
    RefSeqi NP_593825.1. NM_001019254.2.

    3D structure databases

    ProteinModelPortali P30655.
    SMRi P30655. Positions 62-270.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 280037. 2 interactions.
    MINTi MINT-4688233.
    STRINGi 4896.SPAC4A8.13c-1.

    Protein family/group databases

    MEROPSi T01.012.

    Proteomic databases

    MaxQBi P30655.
    PaxDbi P30655.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC4A8.13c.1 ; SPAC4A8.13c.1:pep ; SPAC4A8.13c .
    GeneIDi 2543623.
    KEGGi spo:SPAC4A8.13c.

    Organism-specific databases

    PomBasei SPAC4A8.13c.

    Phylogenomic databases

    eggNOGi COG0638.
    HOGENOMi HOG000091082.
    KOi K02737.
    OMAi TMCAGVT.
    OrthoDBi EOG70W3R5.
    PhylomeDBi P30655.

    Enzyme and pathway databases

    Reactomei REACT_188524. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_188566. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_188568. ER-Phagosome pathway.
    REACT_188587. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_208775. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_215140. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_215320. Orc1 removal from chromatin.
    REACT_218991. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_81558. Regulation of activated PAK-2p34 by proteasome mediated degradation.

    Miscellaneous databases

    NextBioi 20804629.
    PROi P30655.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    PRINTSi PR00141. PROTEASOME.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Stone E.M., Tanaka K., Ichihara A., Yanagida M.
      Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. Hilti N.
      Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-204.
      Strain: 972 / ATCC 24843.
    4. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPSB5_SCHPO
    AccessioniPrimary (citable) accession number: P30655
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 126 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3