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Protein

Probable proteasome subunit beta type-5

Gene

pts1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei62 – 621NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiREACT_282727. Orc1 removal from chromatin.
REACT_294541. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_308182. SCF-beta-TrCP mediated degradation of Emi1.
REACT_310263. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_327472. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_329750. ER-Phagosome pathway.
REACT_331986. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_349687. Cross-presentation of soluble exogenous antigens (endosomes).

Protein family/group databases

MEROPSiT01.012.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable proteasome subunit beta type-5 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit pts1
Multicatalytic endopeptidase complex subunit pts1
Proteasome component pts1
Gene namesi
Name:pts1
ORF Names:SPAC4A8.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4A8.13c.
PomBaseiSPAC4A8.13c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: PomBase
  • nuclear envelope Source: PomBase
  • nucleus Source: PomBase
  • proteasome core complex Source: GO_Central
  • proteasome core complex, beta-subunit complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 6161Removed in mature formBy similarityPRO_0000026609Add
BLAST
Chaini62 – 272211Probable proteasome subunit beta type-5PRO_0000026610Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

MaxQBiP30655.
PaxDbiP30655.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity).By similarity

Protein-protein interaction databases

BioGridi280037. 2 interactions.
MINTiMINT-4688233.
STRINGi4896.SPAC4A8.13c-1.

Structurei

3D structure databases

ProteinModelPortaliP30655.
SMRiP30655. Positions 62-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091082.
InParanoidiP30655.
KOiK02737.
OMAiTMCAGVT.
OrthoDBiEOG70W3R5.
PhylomeDBiP30655.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30655-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSIVSKYTQ STNNDDPKKI IEEEGFTNRF DVVPVPQSSL YLRNLTDETK
60 70 80 90 100
NKHCLIKMNH GTTTLAFRYQ HGIVVCVDSR ASAGPLIASQ TVKKVIEINP
110 120 130 140 150
YLLGTLAGGA ADCQFWETVL GMECRLHQLR NKELISVSAA SKILSNITYS
160 170 180 190 200
YKGYGLSMGT MLAGTGKGGT ALYYIDSDGT RLKGDLFSVG SGSTFAYGVL
210 220 230 240 250
DSGYRWDLSK QEALYLAQRS IVAATHRDAY SGGSVNLYHI DENGWVFHGN
260 270
FDVDSLIWEA KDNENSFAHI PR
Length:272
Mass (Da):29,987
Last modified:July 15, 1998 - v3
Checksum:i3FC37D02A06B5E61
GO

Sequence cautioni

The sequence CAA58746.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13094 Genomic DNA. Translation: BAA02403.1.
CU329670 Genomic DNA. Translation: CAB11483.1.
X83866 Genomic DNA. Translation: CAA58746.1. Different initiation.
PIRiJS0753.
T38783.
RefSeqiNP_593825.1. NM_001019254.2.

Genome annotation databases

EnsemblFungiiSPAC4A8.13c.1; SPAC4A8.13c.1:pep; SPAC4A8.13c.
GeneIDi2543623.
KEGGispo:SPAC4A8.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13094 Genomic DNA. Translation: BAA02403.1.
CU329670 Genomic DNA. Translation: CAB11483.1.
X83866 Genomic DNA. Translation: CAA58746.1. Different initiation.
PIRiJS0753.
T38783.
RefSeqiNP_593825.1. NM_001019254.2.

3D structure databases

ProteinModelPortaliP30655.
SMRiP30655. Positions 62-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280037. 2 interactions.
MINTiMINT-4688233.
STRINGi4896.SPAC4A8.13c-1.

Protein family/group databases

MEROPSiT01.012.

Proteomic databases

MaxQBiP30655.
PaxDbiP30655.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4A8.13c.1; SPAC4A8.13c.1:pep; SPAC4A8.13c.
GeneIDi2543623.
KEGGispo:SPAC4A8.13c.

Organism-specific databases

EuPathDBiFungiDB:SPAC4A8.13c.
PomBaseiSPAC4A8.13c.

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091082.
InParanoidiP30655.
KOiK02737.
OMAiTMCAGVT.
OrthoDBiEOG70W3R5.
PhylomeDBiP30655.

Enzyme and pathway databases

ReactomeiREACT_282727. Orc1 removal from chromatin.
REACT_294541. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_308182. SCF-beta-TrCP mediated degradation of Emi1.
REACT_310263. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_327472. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_329750. ER-Phagosome pathway.
REACT_331986. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_349687. Cross-presentation of soluble exogenous antigens (endosomes).

Miscellaneous databases

NextBioi20804629.
PROiP30655.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Stone E.M., Tanaka K., Ichihara A., Yanagida M.
    Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Hilti N.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-204.
    Strain: 972 / ATCC 24843.
  4. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPSB5_SCHPO
AccessioniPrimary (citable) accession number: P30655
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 15, 1998
Last modified: May 27, 2015
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.