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P30655

- PSB5_SCHPO

UniProt

P30655 - PSB5_SCHPO

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Protein
Probable proteasome subunit beta type-5
Gene
pts1, SPAC4A8.13c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei62 – 621Nucleophile By similarity

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. proteasome-mediated ubiquitin-dependent protein catabolic process Source: PomBase
  2. regulation of mitotic cell cycle Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiREACT_188524. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_188566. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_188568. ER-Phagosome pathway.
REACT_188587. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_208775. SCF-beta-TrCP mediated degradation of Emi1.
REACT_215140. CDK-mediated phosphorylation and removal of Cdc6.
REACT_215320. Orc1 removal from chromatin.
REACT_218991. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_81558. Regulation of activated PAK-2p34 by proteasome mediated degradation.

Protein family/group databases

MEROPSiT01.012.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable proteasome subunit beta type-5 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit pts1
Multicatalytic endopeptidase complex subunit pts1
Proteasome component pts1
Gene namesi
Name:pts1
ORF Names:SPAC4A8.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC4A8.13c.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. nuclear envelope Source: PomBase
  3. nucleus Source: PomBase
  4. proteasome core complex, beta-subunit complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 6161Removed in mature form By similarity
PRO_0000026609Add
BLAST
Chaini62 – 272211Probable proteasome subunit beta type-5
PRO_0000026610Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

MaxQBiP30655.
PaxDbiP30655.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.

Protein-protein interaction databases

BioGridi280037. 2 interactions.
MINTiMINT-4688233.
STRINGi4896.SPAC4A8.13c-1.

Structurei

3D structure databases

ProteinModelPortaliP30655.
SMRiP30655. Positions 62-270.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091082.
KOiK02737.
OMAiTMCAGVT.
OrthoDBiEOG70W3R5.
PhylomeDBiP30655.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30655-1 [UniParc]FASTAAdd to Basket

« Hide

MNSIVSKYTQ STNNDDPKKI IEEEGFTNRF DVVPVPQSSL YLRNLTDETK    50
NKHCLIKMNH GTTTLAFRYQ HGIVVCVDSR ASAGPLIASQ TVKKVIEINP 100
YLLGTLAGGA ADCQFWETVL GMECRLHQLR NKELISVSAA SKILSNITYS 150
YKGYGLSMGT MLAGTGKGGT ALYYIDSDGT RLKGDLFSVG SGSTFAYGVL 200
DSGYRWDLSK QEALYLAQRS IVAATHRDAY SGGSVNLYHI DENGWVFHGN 250
FDVDSLIWEA KDNENSFAHI PR 272
Length:272
Mass (Da):29,987
Last modified:July 15, 1998 - v3
Checksum:i3FC37D02A06B5E61
GO

Sequence cautioni

The sequence CAA58746.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13094 Genomic DNA. Translation: BAA02403.1.
CU329670 Genomic DNA. Translation: CAB11483.1.
X83866 Genomic DNA. Translation: CAA58746.1. Different initiation.
PIRiJS0753.
T38783.
RefSeqiNP_593825.1. NM_001019254.2.

Genome annotation databases

EnsemblFungiiSPAC4A8.13c.1; SPAC4A8.13c.1:pep; SPAC4A8.13c.
GeneIDi2543623.
KEGGispo:SPAC4A8.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13094 Genomic DNA. Translation: BAA02403.1 .
CU329670 Genomic DNA. Translation: CAB11483.1 .
X83866 Genomic DNA. Translation: CAA58746.1 . Different initiation.
PIRi JS0753.
T38783.
RefSeqi NP_593825.1. NM_001019254.2.

3D structure databases

ProteinModelPortali P30655.
SMRi P30655. Positions 62-270.
ModBasei Search...

Protein-protein interaction databases

BioGridi 280037. 2 interactions.
MINTi MINT-4688233.
STRINGi 4896.SPAC4A8.13c-1.

Protein family/group databases

MEROPSi T01.012.

Proteomic databases

MaxQBi P30655.
PaxDbi P30655.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC4A8.13c.1 ; SPAC4A8.13c.1:pep ; SPAC4A8.13c .
GeneIDi 2543623.
KEGGi spo:SPAC4A8.13c.

Organism-specific databases

PomBasei SPAC4A8.13c.

Phylogenomic databases

eggNOGi COG0638.
HOGENOMi HOG000091082.
KOi K02737.
OMAi TMCAGVT.
OrthoDBi EOG70W3R5.
PhylomeDBi P30655.

Enzyme and pathway databases

Reactomei REACT_188524. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_188566. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_188568. ER-Phagosome pathway.
REACT_188587. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_208775. SCF-beta-TrCP mediated degradation of Emi1.
REACT_215140. CDK-mediated phosphorylation and removal of Cdc6.
REACT_215320. Orc1 removal from chromatin.
REACT_218991. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_81558. Regulation of activated PAK-2p34 by proteasome mediated degradation.

Miscellaneous databases

NextBioi 20804629.
PROi P30655.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
PRINTSi PR00141. PROTEASOME.
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Stone E.M., Tanaka K., Ichihara A., Yanagida M.
    Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Hilti N.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-204.
    Strain: 972 / ATCC 24843.
  4. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPSB5_SCHPO
AccessioniPrimary (citable) accession number: P30655
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 15, 1998
Last modified: September 3, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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