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P30655 (PSB5_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable proteasome subunit beta type-5

EC=3.4.25.1
Alternative name(s):
Macropain subunit pts1
Multicatalytic endopeptidase complex subunit pts1
Proteasome component pts1
Gene names
Name:pts1
ORF Names:SPAC4A8.13c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.

Subcellular location

Cytoplasm. Nucleus Ref.4.

Sequence similarities

Belongs to the peptidase T1B family.

Sequence caution

The sequence CAA58746.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 6161Removed in mature form By similarity
PRO_0000026609
Chain62 – 272211Probable proteasome subunit beta type-5
PRO_0000026610

Sites

Active site621Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P30655 [UniParc].

Last modified July 15, 1998. Version 3.
Checksum: 3FC37D02A06B5E61

FASTA27229,987
        10         20         30         40         50         60 
MNSIVSKYTQ STNNDDPKKI IEEEGFTNRF DVVPVPQSSL YLRNLTDETK NKHCLIKMNH 

        70         80         90        100        110        120 
GTTTLAFRYQ HGIVVCVDSR ASAGPLIASQ TVKKVIEINP YLLGTLAGGA ADCQFWETVL 

       130        140        150        160        170        180 
GMECRLHQLR NKELISVSAA SKILSNITYS YKGYGLSMGT MLAGTGKGGT ALYYIDSDGT 

       190        200        210        220        230        240 
RLKGDLFSVG SGSTFAYGVL DSGYRWDLSK QEALYLAQRS IVAATHRDAY SGGSVNLYHI 

       250        260        270 
DENGWVFHGN FDVDSLIWEA KDNENSFAHI PR 

« Hide

References

« Hide 'large scale' references
[1]Stone E.M., Tanaka K., Ichihara A., Yanagida M.
Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[3]Hilti N.
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-204.
Strain: 972 / ATCC 24843.
[4]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13094 Genomic DNA. Translation: BAA02403.1.
CU329670 Genomic DNA. Translation: CAB11483.1.
X83866 Genomic DNA. Translation: CAA58746.1. Different initiation.
PIRJS0753.
T38783.
RefSeqNP_593825.1. NM_001019254.2.

3D structure databases

ProteinModelPortalP30655.
SMRP30655. Positions 62-270.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid280037. 2 interactions.
MINTMINT-4688233.
STRING4896.SPAC4A8.13c-1.

Protein family/group databases

MEROPST01.012.

Proteomic databases

MaxQBP30655.
PaxDbP30655.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC4A8.13c.1; SPAC4A8.13c.1:pep; SPAC4A8.13c.
GeneID2543623.
KEGGspo:SPAC4A8.13c.

Organism-specific databases

PomBaseSPAC4A8.13c.

Phylogenomic databases

eggNOGCOG0638.
HOGENOMHOG000091082.
KOK02737.
OMATMCAGVT.
OrthoDBEOG70W3R5.
PhylomeDBP30655.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSPR00141. PROTEASOME.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20804629.
PROP30655.

Entry information

Entry namePSB5_SCHPO
AccessionPrimary (citable) accession number: P30655
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 15, 1998
Last modified: June 11, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

Peptidase families

Classification of peptidase families and list of entries