Reviewed, UniProtKB/Swiss-Prot P30645 (PDE2_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable 3',5'-cyclic phosphodiesterase pde-2
    EC=3.1.4.17

Gene names

Name:	pde-2
ORF Names:	R08D7.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 1 GAF domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	cGMP
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P30645-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: P30645-2)

The sequence of this isoform differs from the canonical sequence as follows:
     692-734: GTFYSEKQEM...RKQDPRAAEI → DIRSSAKARP...RYFGSSFRWI
     735-859: Missing.

Note: No experimental confirmation available.

Isoform c (identifier: P30645-3)

The sequence of this isoform differs from the canonical sequence as follows:
     48-50: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 859	859	Probable 3',5'-cyclic phosphodiesterase pde-2		PRO_0000198845
Regions
	Domain	177 – 312	136	GAF
Natural variations
	Alternative sequence	48 – 50	3	Missing in isoform c.		VSP_014355
	Alternative sequence	692 – 734	43	GTFYS…RAAEI → DIRSSAKARPKSCRNCFKTV RACICKRKCSSNTRYFGSSF RWI in isoform b.		VSP_011155
	Alternative sequence	735 – 859	125	Missing in isoform b.		VSP_011156

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified July 30, 2004. Version 3. Checksum: 3CDE777017E08455	FASTA	859	97,770
	10 20 30 40 50 60 MLELRRNSSP SSAHPSPQTN CQNSQRGDGL HHHHHEAASG STCCGGMTVF TGANAAKSSN 70 80 90 100 110 120 EPAGSASPTV WRKTSHPPLH FNNNETRNRN LQMQLKNRGT KDDWGASLRY DIEEPTSSGL 130 140 150 160 170 180 LELLPDVPIV RKLSRPLVKM DDQDDACSVA SNESDRTVLS PLVPMSIFDQ FLCLTNNLSA 190 200 210 220 230 240 LISCIIAEAK KNTEAEDYAV FLHDEDNKQM VLFNNETMLM TGKKFDMGYG IVGKVASTMR 250 260 270 280 290 300 TMNIRDVSRC PFFNEEIDEQ FSIKARNLIA FPLIDSSCSL IGVIVLYNKE NGFSRHDEKY 310 320 330 340 350 360 IKRFSYFVAN SIAHAILAKQ IEEVRTRIHM VEEFKIQGED AVIEEVDIMR LVNDPLRDWR 370 380 390 400 410 420 YFSQNFADFS FPPRSVGENH FHRASMMFFE DLGFSMLYKL NKRKLSYLVL RVSAGYRPVP 430 440 450 460 470 480 YHNWSHAFAV THFCWLTLRT DAIRRALSDM ERLSLLIACL CHDIDHRGTT NSFQMQSLQK 490 500 510 520 530 540 TPLSVLYSTE GSVLERHHFA QTIKLLQQEE CSILENLPAA DFRTIVNTIR EVILATDISA 550 560 570 580 590 600 HLRKQERIKT MISEGYNPMS FDHRYLLMCL VMTASDLSDQ AKNFHNAKRI AENIYLEFFA 610 620 630 640 650 660 QGDLELQLGV KPLEMMDRTN AYVPTVQIDF LFKIGVPVFQ LLASVVPEGR TTSEAIDANH 670 680 690 700 710 720 LCWVALDEEV RNNPSATKYT RVFARRESRT EGTFYSEKQE MEICEKPKKL MLEFIFFKTI 730 740 750 760 770 780 YDRVRKQDPR AAEIASKRFE PVYANGSVPQ TQDILDHRFD GYDKKYQIGC SGGQNQSNGK 790 800 810 820 830 840 QQQIRSLQKI RSKTSEDDAL LKPMDNGGSV AASSTRRGSR TPRRLWRRAR QLISSMSSSC 850 ASCSPLPSRR QVSEDSESC
	Isoform b. Checksum: AEDD4F142F1EDD47	FASTA	734	83,694
	10 20 30 40 50 60 MLELRRNSSP SSAHPSPQTN CQNSQRGDGL HHHHHEAASG STCCGGMTVF TGANAAKSSN 70 80 90 100 110 120 EPAGSASPTV WRKTSHPPLH FNNNETRNRN LQMQLKNRGT KDDWGASLRY DIEEPTSSGL 130 140 150 160 170 180 LELLPDVPIV RKLSRPLVKM DDQDDACSVA SNESDRTVLS PLVPMSIFDQ FLCLTNNLSA 190 200 210 220 230 240 LISCIIAEAK KNTEAEDYAV FLHDEDNKQM VLFNNETMLM TGKKFDMGYG IVGKVASTMR 250 260 270 280 290 300 TMNIRDVSRC PFFNEEIDEQ FSIKARNLIA FPLIDSSCSL IGVIVLYNKE NGFSRHDEKY 310 320 330 340 350 360 IKRFSYFVAN SIAHAILAKQ IEEVRTRIHM VEEFKIQGED AVIEEVDIMR LVNDPLRDWR 370 380 390 400 410 420 YFSQNFADFS FPPRSVGENH FHRASMMFFE DLGFSMLYKL NKRKLSYLVL RVSAGYRPVP 430 440 450 460 470 480 YHNWSHAFAV THFCWLTLRT DAIRRALSDM ERLSLLIACL CHDIDHRGTT NSFQMQSLQK 490 500 510 520 530 540 TPLSVLYSTE GSVLERHHFA QTIKLLQQEE CSILENLPAA DFRTIVNTIR EVILATDISA 550 560 570 580 590 600 HLRKQERIKT MISEGYNPMS FDHRYLLMCL VMTASDLSDQ AKNFHNAKRI AENIYLEFFA 610 620 630 640 650 660 QGDLELQLGV KPLEMMDRTN AYVPTVQIDF LFKIGVPVFQ LLASVVPEGR TTSEAIDANH 670 680 690 700 710 720 LCWVALDEEV RNNPSATKYT RVFARRESRT EDIRSSAKAR PKSCRNCFKT VRACICKRKC 730 SSNTRYFGSS FRWI
	Isoform c. Checksum: 12F2DCDCA3043578	FASTA	856	97,423
	10 20 30 40 50 60 MLELRRNSSP SSAHPSPQTN CQNSQRGDGL HHHHHEAASG STCCGGMTGA NAAKSSNEPA 70 80 90 100 110 120 GSASPTVWRK TSHPPLHFNN NETRNRNLQM QLKNRGTKDD WGASLRYDIE EPTSSGLLEL 130 140 150 160 170 180 LPDVPIVRKL SRPLVKMDDQ DDACSVASNE SDRTVLSPLV PMSIFDQFLC LTNNLSALIS 190 200 210 220 230 240 CIIAEAKKNT EAEDYAVFLH DEDNKQMVLF NNETMLMTGK KFDMGYGIVG KVASTMRTMN 250 260 270 280 290 300 IRDVSRCPFF NEEIDEQFSI KARNLIAFPL IDSSCSLIGV IVLYNKENGF SRHDEKYIKR 310 320 330 340 350 360 FSYFVANSIA HAILAKQIEE VRTRIHMVEE FKIQGEDAVI EEVDIMRLVN DPLRDWRYFS 370 380 390 400 410 420 QNFADFSFPP RSVGENHFHR ASMMFFEDLG FSMLYKLNKR KLSYLVLRVS AGYRPVPYHN 430 440 450 460 470 480 WSHAFAVTHF CWLTLRTDAI RRALSDMERL SLLIACLCHD IDHRGTTNSF QMQSLQKTPL 490 500 510 520 530 540 SVLYSTEGSV LERHHFAQTI KLLQQEECSI LENLPAADFR TIVNTIREVI LATDISAHLR 550 560 570 580 590 600 KQERIKTMIS EGYNPMSFDH RYLLMCLVMT ASDLSDQAKN FHNAKRIAEN IYLEFFAQGD 610 620 630 640 650 660 LELQLGVKPL EMMDRTNAYV PTVQIDFLFK IGVPVFQLLA SVVPEGRTTS EAIDANHLCW 670 680 690 700 710 720 VALDEEVRNN PSATKYTRVF ARRESRTEGT FYSEKQEMEI CEKPKKLMLE FIFFKTIYDR 730 740 750 760 770 780 VRKQDPRAAE IASKRFEPVY ANGSVPQTQD ILDHRFDGYD KKYQIGCSGG QNQSNGKQQQ 790 800 810 820 830 840 IRSLQKIRSK TSEDDALLKP MDNGGSVAAS STRRGSRTPR RLWRRARQLI SSMSSSCASC 850 SPLPSRRQVS EDSESC

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z12017 Genomic DNA. Translation: CAA78052.2. Z12017 Genomic DNA. Translation: CAE47469.1. Z12017 Genomic DNA. Translation: CAH19088.1.
PIR	D88544. S24462. S41041.
RefSeq	NP_001022705.1. NP_001022706.1. NP_001022707.1.
UniGene	Cel.6004
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P30645.
Genome annotation databases
Ensembl	R08D7.6a; R08D7.6a; R08D7.6; Caenorhabditis elegans. [Genome view] R08D7.6b; R08D7.6b; R08D7.6; Caenorhabditis elegans. [Genome view] R08D7.6c; R08D7.6c; R08D7.6; Caenorhabditis elegans. [Genome view]
GeneID	176270.
KEGG	cel:R08D7.6.
UCSC	R08D7.6b. c. elegans.
Organism-specific databases
CTD	176270.
WormBase	WBGene00011146. pde-2.
WormPep	R08D7.6a. CE35749. [WorfDB] R08D7.6b. CE35750. [WorfDB] R08D7.6c. CE37267. [WorfDB]
Phylogenomic databases
OMA	ECSILEN.
Enzyme and pathway databases
BRENDA	3.1.4.17. 672.
Gene expression databases
ArrayExpress	P30645.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF01590. GAF. 1 hit. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 1 hit. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	891854.

Entry information

Entry name

PDE2_CAEEL

Accession

Primary (citable) accession number: P30645
Secondary accession number(s): Q65ZC7, Q7JMU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	July 30, 2004
Last modified:	November 3, 2009
This is version 64 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project