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Protein

Probable 3',5'-cyclic phosphodiesterase pde-2

Gene

pde-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei422 – 4221Proton donorBy similarity
Metal bindingi426 – 4261Divalent metal cation 1By similarity
Metal bindingi462 – 4621Divalent metal cation 1By similarity
Metal bindingi463 – 4631Divalent metal cation 1By similarity
Metal bindingi463 – 4631Divalent metal cation 2By similarity
Metal bindingi576 – 5761Divalent metal cation 1By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-nucleotide phosphodiesterase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_237493. cGMP effects.
REACT_255560. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 3',5'-cyclic phosphodiesterase pde-2 (EC:3.1.4.17)
Gene namesi
Name:pde-2
ORF Names:R08D7.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiR08D7.6a; CE44867; WBGene00011146; pde-2.
R08D7.6b; CE44922; WBGene00011146; pde-2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 831831Probable 3',5'-cyclic phosphodiesterase pde-2PRO_0000198845Add
BLAST

Proteomic databases

PaxDbiP30645.
PRIDEiP30645.

Structurei

3D structure databases

ProteinModelPortaliP30645.
SMRiP30645. Positions 135-683.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini177 – 312136GAFAdd
BLAST

Sequence similaritiesi

Contains 1 GAF domain.Curated

Phylogenomic databases

eggNOGiNOG270709.
GeneTreeiENSGT00760000119066.
HOGENOMiHOG000018108.
InParanoidiP30645.
KOiK18283.
OMAiRYDIEEP.
OrthoDBiEOG767393.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 1 hit.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 1 hit.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: P30645-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLELRRNSSP SSAHPSPQTN CQNSQRGDGL HHHHHEAASG STCCGGMTVF
60 70 80 90 100
TGANAAKSSN EPAGSASPTV WRKTSHPPLH FNNNETRNRN LQMQLKNRGT
110 120 130 140 150
KDDWGASLRY DIEEPTSSGL LELLPDVPIV RKLSRPLVKM DDQDDACSVA
160 170 180 190 200
SNESDRTVLS PLVPMSIFDQ FLCLTNNLSA LISCIIAEAK KNTEAEDYAV
210 220 230 240 250
FLHDEDNKQM VLFNNETMLM TGKKFDMGYG IVGKVASTMR TMNIRDVSRC
260 270 280 290 300
PFFNEEIDEQ FSIKARNLIA FPLIDSSCSL IGVIVLYNKE NGFSRHDEKY
310 320 330 340 350
IKRFSYFVAN SIAHAILAKQ IEEVRTRIHM VEEFKIQGED AVIEEVDIMR
360 370 380 390 400
LVNDPLRDWR YFSQNFADFS FPPRSVGENH FHRASMMFFE DLGFSMLYKL
410 420 430 440 450
NKRKLSYLVL RVSAGYRPVP YHNWSHAFAV THFCWLTLRT DAIRRALSDM
460 470 480 490 500
ERLSLLIACL CHDIDHRGTT NSFQMQSLQK TPLSVLYSTE GSVLERHHFA
510 520 530 540 550
QTIKLLQQEE CSILENLPAA DFRTIVNTIR EVILATDISA HLRKQERIKT
560 570 580 590 600
MISEGYNPMS FDHRYLLMCL VMTASDLSDQ AKNFHNAKRI AENIYLEFFA
610 620 630 640 650
QGDLELQLGV KPLEMMDRTN AYVPTVQIDF LFKIGVPVFQ LLASVVPEGR
660 670 680 690 700
TTSEAIDANH LCWVALDEEV RNNPSATNTL EYLRDENLER RIYDRVRKQD
710 720 730 740 750
PRAAEIASKR FEPVYANGSV PQTQDILDHR FDGYDKKYQI GCSGGQNQSN
760 770 780 790 800
GKQQQIRSLQ KIRSKTSEDD ALLKPMDNGG SVAASSTRRG SRTPRRLWRR
810 820 830
ARQLISSMSS SCASCSPLPS RRQVSEDSES C

Note: No experimental confirmation available.

Length:831
Mass (Da):94,364
Last modified:July 27, 2011 - v4
Checksum:i0BCD803345E113B1
GO
Isoform b (identifier: P30645-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     48-50: Missing.

Note: No experimental confirmation available.

Show »
Length:828
Mass (Da):94,017
Checksum:i6C8B0F649C7E09D5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei48 – 503Missing in isoform b. CuratedVSP_014355

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12017 Genomic DNA. Translation: CAA78052.3.
Z12017 Genomic DNA. Translation: CAE47469.2.
PIRiD88544.
S41041. S24462.
RefSeqiNP_001022705.2. NM_001027534.3. [P30645-1]
NP_001022706.2. NM_001027535.4. [P30645-3]
UniGeneiCel.6004.

Genome annotation databases

EnsemblMetazoaiR08D7.6a; R08D7.6a; WBGene00011146. [P30645-1]
GeneIDi176270.
KEGGicel:CELE_R08D7.6.
UCSCiR08D7.6b. c. elegans. [P30645-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12017 Genomic DNA. Translation: CAA78052.3.
Z12017 Genomic DNA. Translation: CAE47469.2.
PIRiD88544.
S41041. S24462.
RefSeqiNP_001022705.2. NM_001027534.3. [P30645-1]
NP_001022706.2. NM_001027535.4. [P30645-3]
UniGeneiCel.6004.

3D structure databases

ProteinModelPortaliP30645.
SMRiP30645. Positions 135-683.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP30645.
PRIDEiP30645.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR08D7.6a; R08D7.6a; WBGene00011146. [P30645-1]
GeneIDi176270.
KEGGicel:CELE_R08D7.6.
UCSCiR08D7.6b. c. elegans. [P30645-1]

Organism-specific databases

CTDi176270.
WormBaseiR08D7.6a; CE44867; WBGene00011146; pde-2.
R08D7.6b; CE44922; WBGene00011146; pde-2.

Phylogenomic databases

eggNOGiNOG270709.
GeneTreeiENSGT00760000119066.
HOGENOMiHOG000018108.
InParanoidiP30645.
KOiK18283.
OMAiRYDIEEP.
OrthoDBiEOG767393.

Enzyme and pathway databases

ReactomeiREACT_237493. cGMP effects.
REACT_255560. G alpha (s) signalling events.

Miscellaneous databases

NextBioi891854.
PROiP30645.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 1 hit.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 1 hit.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiPDE2_CAEEL
AccessioniPrimary (citable) accession number: P30645
Secondary accession number(s): Q65ZC7, Q7JMU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 27, 2011
Last modified: January 7, 2015
This is version 107 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.