P30645 (PDE2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3',5'-cyclic phosphodiesterase pde-2
EC=3.1.4.17

Gene names

Name:	pde-2
ORF Names:	R08D7.6

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	831 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 1 GAF domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC signal transduction Inferred from electronic annotation. Source: InterPro
Molecular_function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P30645-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: P30645-3)

The sequence of this isoform differs from the canonical sequence as follows:
     48-50: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 831	831	Probable 3',5'-cyclic phosphodiesterase pde-2		PRO_0000198845
Regions
	Domain	177 – 312	136	GAF
Sites
	Active site	422	1	Proton donor By similarity
	Metal binding	426	1	Divalent metal cation 1 By similarity
	Metal binding	462	1	Divalent metal cation 1 By similarity
	Metal binding	463	1	Divalent metal cation 1 By similarity
	Metal binding	463	1	Divalent metal cation 2 By similarity
	Metal binding	576	1	Divalent metal cation 1 By similarity
Natural variations
	Alternative sequence	48 – 50	3	Missing in isoform b.		VSP_014355

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified July 27, 2011. Version 4. Checksum: 0BCD803345E113B1	FASTA	831	94,364
	10 20 30 40 50 60 MLELRRNSSP SSAHPSPQTN CQNSQRGDGL HHHHHEAASG STCCGGMTVF TGANAAKSSN 70 80 90 100 110 120 EPAGSASPTV WRKTSHPPLH FNNNETRNRN LQMQLKNRGT KDDWGASLRY DIEEPTSSGL 130 140 150 160 170 180 LELLPDVPIV RKLSRPLVKM DDQDDACSVA SNESDRTVLS PLVPMSIFDQ FLCLTNNLSA 190 200 210 220 230 240 LISCIIAEAK KNTEAEDYAV FLHDEDNKQM VLFNNETMLM TGKKFDMGYG IVGKVASTMR 250 260 270 280 290 300 TMNIRDVSRC PFFNEEIDEQ FSIKARNLIA FPLIDSSCSL IGVIVLYNKE NGFSRHDEKY 310 320 330 340 350 360 IKRFSYFVAN SIAHAILAKQ IEEVRTRIHM VEEFKIQGED AVIEEVDIMR LVNDPLRDWR 370 380 390 400 410 420 YFSQNFADFS FPPRSVGENH FHRASMMFFE DLGFSMLYKL NKRKLSYLVL RVSAGYRPVP 430 440 450 460 470 480 YHNWSHAFAV THFCWLTLRT DAIRRALSDM ERLSLLIACL CHDIDHRGTT NSFQMQSLQK 490 500 510 520 530 540 TPLSVLYSTE GSVLERHHFA QTIKLLQQEE CSILENLPAA DFRTIVNTIR EVILATDISA 550 560 570 580 590 600 HLRKQERIKT MISEGYNPMS FDHRYLLMCL VMTASDLSDQ AKNFHNAKRI AENIYLEFFA 610 620 630 640 650 660 QGDLELQLGV KPLEMMDRTN AYVPTVQIDF LFKIGVPVFQ LLASVVPEGR TTSEAIDANH 670 680 690 700 710 720 LCWVALDEEV RNNPSATNTL EYLRDENLER RIYDRVRKQD PRAAEIASKR FEPVYANGSV 730 740 750 760 770 780 PQTQDILDHR FDGYDKKYQI GCSGGQNQSN GKQQQIRSLQ KIRSKTSEDD ALLKPMDNGG 790 800 810 820 830 SVAASSTRRG SRTPRRLWRR ARQLISSMSS SCASCSPLPS RRQVSEDSES C
	Isoform b [UniParc]. Checksum: 6C8B0F649C7E09D5	FASTA	828	94,017
	10 20 30 40 50 60 MLELRRNSSP SSAHPSPQTN CQNSQRGDGL HHHHHEAASG STCCGGMTGA NAAKSSNEPA 70 80 90 100 110 120 GSASPTVWRK TSHPPLHFNN NETRNRNLQM QLKNRGTKDD WGASLRYDIE EPTSSGLLEL 130 140 150 160 170 180 LPDVPIVRKL SRPLVKMDDQ DDACSVASNE SDRTVLSPLV PMSIFDQFLC LTNNLSALIS 190 200 210 220 230 240 CIIAEAKKNT EAEDYAVFLH DEDNKQMVLF NNETMLMTGK KFDMGYGIVG KVASTMRTMN 250 260 270 280 290 300 IRDVSRCPFF NEEIDEQFSI KARNLIAFPL IDSSCSLIGV IVLYNKENGF SRHDEKYIKR 310 320 330 340 350 360 FSYFVANSIA HAILAKQIEE VRTRIHMVEE FKIQGEDAVI EEVDIMRLVN DPLRDWRYFS 370 380 390 400 410 420 QNFADFSFPP RSVGENHFHR ASMMFFEDLG FSMLYKLNKR KLSYLVLRVS AGYRPVPYHN 430 440 450 460 470 480 WSHAFAVTHF CWLTLRTDAI RRALSDMERL SLLIACLCHD IDHRGTTNSF QMQSLQKTPL 490 500 510 520 530 540 SVLYSTEGSV LERHHFAQTI KLLQQEECSI LENLPAADFR TIVNTIREVI LATDISAHLR 550 560 570 580 590 600 KQERIKTMIS EGYNPMSFDH RYLLMCLVMT ASDLSDQAKN FHNAKRIAEN IYLEFFAQGD 610 620 630 640 650 660 LELQLGVKPL EMMDRTNAYV PTVQIDFLFK IGVPVFQLLA SVVPEGRTTS EAIDANHLCW 670 680 690 700 710 720 VALDEEVRNN PSATNTLEYL RDENLERRIY DRVRKQDPRA AEIASKRFEP VYANGSVPQT 730 740 750 760 770 780 QDILDHRFDG YDKKYQIGCS GGQNQSNGKQ QQIRSLQKIR SKTSEDDALL KPMDNGGSVA 790 800 810 820 ASSTRRGSRT PRRLWRRARQ LISSMSSSCA SCSPLPSRRQ VSEDSESC

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z12017 Genomic DNA. Translation: CAA78052.3. Z12017 Genomic DNA. Translation: CAE47469.2.
PIR	D88544. S24462. S41041.
RefSeq	NP_001022705.2. NM_001027534.3. NP_001022706.2. NM_001027535.4.
3D structure databases
ProteinModelPortal	P30645.
SMR	P30645. Positions 135-683.
ModBase	Search...
Proteomic databases
PaxDb	P30645.
PRIDE	P30645.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	R08D7.6a; R08D7.6a; R08D7.6.
GeneID	176270.
KEGG	cel:CELE_R08D7.6.
UCSC	R08D7.6b. c. elegans.
Organism-specific databases
CTD	176270.
WormBase	R08D7.6a; CE44867; WBGene00011146; pde-2. R08D7.6b; CE44922; WBGene00011146; pde-2.
Phylogenomic databases
eggNOG	NOG270709.
GeneTree	ENSGT00550000074280.
HOGENOM	HOG000018108.
InParanoid	P30645.
OMA	RYDIEEP.
Family and domain databases
Gene3D	1.10.1300.10. 1 hit.
InterPro	IPR003018. GAF. IPR003607. HD/PDEase_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Pfam	PF01590. GAF. 1 hit. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 1 hit. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	891854.

Entry information

Entry name

PDE2_CAEEL

Accession

Primary (citable) accession number: P30645
Secondary accession number(s): Q65ZC7, Q7JMU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 94 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project