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Protein

Thiamin pyrophosphokinase 1

Gene

tpk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Functions cell non-autonomously.1 Publication

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kinase activity Source: UniProtKB-KW
  3. thiamine binding Source: InterPro
  4. thiamine diphosphokinase activity Source: UniProtKB-EC

GO - Biological processi

  1. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
  2. thiamine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.6.2. 1045.
ReactomeiREACT_311537. Vitamin B1 (thiamin) metabolism.
UniPathwayiUPA00060; UER00597.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamin pyrophosphokinase 1 (EC:2.7.6.2)
Alternative name(s):
Thiamine pyrophosphokinase 1
Short name:
TPK1
Gene namesi
Name:tpk-1
ORF Names:ZK637.9
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiZK637.9a; CE37946; WBGene00014027; tpk-1.
ZK637.9b; CE37947; WBGene00014027; tpk-1.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi67 – 671C → Y in qm162; maternal-effect mutation. Partial loss-of-function. Slowdown and deregulation of physiological rhythms and lengthening of lifespan. No effect on oxygen consumption rate. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243Thiamin pyrophosphokinase 1PRO_0000072646Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP30636.
SMRiP30636. Positions 19-219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.Curated

Phylogenomic databases

eggNOGiCOG1564.
GeneTreeiENSGT00390000016016.
HOGENOMiHOG000018120.
InParanoidiP30636.
KOiK00949.
OMAiWEFENIV.
OrthoDBiEOG76HQ2J.
PhylomeDBiP30636.

Family and domain databases

Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
InterProiIPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamiPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b (identifier: P30636-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKKLKPFEI LEDSCASVCI WLNGEPTAIS NRAENLWNKA KYRVATDGAV
60 70 80 90 100
NEILKRKSFV EWPHIICGDF DSINKQIDTK NAKVVHLPDQ DYTDLSKSVQ
110 120 130 140 150
WCLEQKTLTS WEFENIVVLG GLNGRFDHTM STLSSLIRFV DSQTPVIVLD
160 170 180 190 200
SRNLVLAVPT GDSNLDVNLE MTTKMCGIIP IVQKETIVSS IGLKYEMENL
210 220 230 240
ALEFGKLIST SNEVTTSQVF LKSSSSLIFS IELENWVYKL DSL

Note: No experimental confirmation available.

Length:243
Mass (Da):27,314
Last modified:April 26, 2005 - v3
Checksum:i2BA5401B76326678
GO
Isoform a (identifier: P30636-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     146-160: Missing.

Show »
Length:228
Mass (Da):25,723
Checksum:i78E3536535C2CA6C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei146 – 16015Missing in isoform a. 1 PublicationVSP_013462Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY513235 mRNA. Translation: AAS21678.1.
Z11115 Genomic DNA. Translation: CAA77455.3.
Z11115 Genomic DNA. Translation: CAI46594.1.
PIRiS15797.
RefSeqiNP_001023023.1. NM_001027852.1. [P30636-2]
NP_001023024.1. NM_001027853.1. [P30636-1]
UniGeneiCel.9539.

Genome annotation databases

EnsemblMetazoaiZK637.9b; ZK637.9b; WBGene00014027. [P30636-1]
GeneIDi176258.
KEGGicel:CELE_ZK637.9.
UCSCiZK637.9b. c. elegans. [P30636-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY513235 mRNA. Translation: AAS21678.1.
Z11115 Genomic DNA. Translation: CAA77455.3.
Z11115 Genomic DNA. Translation: CAI46594.1.
PIRiS15797.
RefSeqiNP_001023023.1. NM_001027852.1. [P30636-2]
NP_001023024.1. NM_001027853.1. [P30636-1]
UniGeneiCel.9539.

3D structure databases

ProteinModelPortaliP30636.
SMRiP30636. Positions 19-219.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZK637.9b; ZK637.9b; WBGene00014027. [P30636-1]
GeneIDi176258.
KEGGicel:CELE_ZK637.9.
UCSCiZK637.9b. c. elegans. [P30636-1]

Organism-specific databases

CTDi176258.
WormBaseiZK637.9a; CE37946; WBGene00014027; tpk-1.
ZK637.9b; CE37947; WBGene00014027; tpk-1.

Phylogenomic databases

eggNOGiCOG1564.
GeneTreeiENSGT00390000016016.
HOGENOMiHOG000018120.
InParanoidiP30636.
KOiK00949.
OMAiWEFENIV.
OrthoDBiEOG76HQ2J.
PhylomeDBiP30636.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00597.
BRENDAi2.7.6.2. 1045.
ReactomeiREACT_311537. Vitamin B1 (thiamin) metabolism.

Miscellaneous databases

NextBioi891808.
PROiP30636.

Family and domain databases

Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
InterProiIPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamiPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Thiamine pyrophosphate biosynthesis and transport in the nematode Caenorhabditis elegans."
    de Jong L., Meng Y., Dent J., Hekimi S.
    Genetics 168:845-854(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, MUTAGENESIS OF CYS-67.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiTPK1_CAEEL
AccessioniPrimary (citable) accession number: P30636
Secondary accession number(s): Q5FC86, Q6R5A6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 26, 2005
Last modified: April 1, 2015
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.