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P30636

- TPK1_CAEEL

UniProt

P30636 - TPK1_CAEEL

Protein

Thiamin pyrophosphokinase 1

Gene

tpk-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 3 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Functions cell non-autonomously.1 Publication

    Catalytic activityi

    ATP + thiamine = AMP + thiamine diphosphate.

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. kinase activity Source: UniProtKB-KW
    3. thiamine diphosphokinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    2. thiamine metabolic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00060; UER00597.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thiamin pyrophosphokinase 1 (EC:2.7.6.2)
    Alternative name(s):
    Thiamine pyrophosphokinase 1
    Short name:
    TPK1
    Gene namesi
    Name:tpk-1
    ORF Names:ZK637.9
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiZK637.9a; CE37946; WBGene00014027; tpk-1.
    ZK637.9b; CE37947; WBGene00014027; tpk-1.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi67 – 671C → Y in qm162; maternal-effect mutation. Partial loss-of-function. Slowdown and deregulation of physiological rhythms and lengthening of lifespan. No effect on oxygen consumption rate. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 243243Thiamin pyrophosphokinase 1PRO_0000072646Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP30636.
    SMRiP30636. Positions 19-219.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thiamine pyrophosphokinase family.Curated

    Phylogenomic databases

    eggNOGiCOG1564.
    GeneTreeiENSGT00390000016016.
    HOGENOMiHOG000018120.
    InParanoidiP30636.
    KOiK00949.
    OMAiWEFENIV.
    OrthoDBiEOG76HQ2J.
    PhylomeDBiP30636.

    Family and domain databases

    Gene3Di2.60.120.320. 1 hit.
    3.40.50.10240. 1 hit.
    InterProiIPR006282. Thi_PPkinase.
    IPR016966. Thiamin_pyrophosphokinase_euk.
    IPR007373. Thiamin_PyroPKinase_B1-bd.
    IPR007371. TPK_catalytic.
    [Graphical view]
    PfamiPF04265. TPK_B1_binding. 1 hit.
    PF04263. TPK_catalytic. 1 hit.
    [Graphical view]
    PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
    SMARTiSM00983. TPK_B1_binding. 1 hit.
    [Graphical view]
    SUPFAMiSSF63862. SSF63862. 1 hit.
    SSF63999. SSF63999. 1 hit.
    TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform b (identifier: P30636-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSKKLKPFEI LEDSCASVCI WLNGEPTAIS NRAENLWNKA KYRVATDGAV    50
    NEILKRKSFV EWPHIICGDF DSINKQIDTK NAKVVHLPDQ DYTDLSKSVQ 100
    WCLEQKTLTS WEFENIVVLG GLNGRFDHTM STLSSLIRFV DSQTPVIVLD 150
    SRNLVLAVPT GDSNLDVNLE MTTKMCGIIP IVQKETIVSS IGLKYEMENL 200
    ALEFGKLIST SNEVTTSQVF LKSSSSLIFS IELENWVYKL DSL 243

    Note: No experimental confirmation available.

    Length:243
    Mass (Da):27,314
    Last modified:April 26, 2005 - v3
    Checksum:i2BA5401B76326678
    GO
    Isoform a (identifier: P30636-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         146-160: Missing.

    Show »
    Length:228
    Mass (Da):25,723
    Checksum:i78E3536535C2CA6C
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei146 – 16015Missing in isoform a. 1 PublicationVSP_013462Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY513235 mRNA. Translation: AAS21678.1.
    Z11115 Genomic DNA. Translation: CAA77455.3.
    Z11115 Genomic DNA. Translation: CAI46594.1.
    PIRiS15797.
    RefSeqiNP_001023023.1. NM_001027852.1. [P30636-2]
    NP_001023024.1. NM_001027853.1. [P30636-1]
    UniGeneiCel.9539.

    Genome annotation databases

    EnsemblMetazoaiZK637.9b; ZK637.9b; WBGene00014027. [P30636-1]
    GeneIDi176258.
    KEGGicel:CELE_ZK637.9.
    UCSCiZK637.9b. c. elegans. [P30636-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY513235 mRNA. Translation: AAS21678.1 .
    Z11115 Genomic DNA. Translation: CAA77455.3 .
    Z11115 Genomic DNA. Translation: CAI46594.1 .
    PIRi S15797.
    RefSeqi NP_001023023.1. NM_001027852.1. [P30636-2 ]
    NP_001023024.1. NM_001027853.1. [P30636-1 ]
    UniGenei Cel.9539.

    3D structure databases

    ProteinModelPortali P30636.
    SMRi P30636. Positions 19-219.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai ZK637.9b ; ZK637.9b ; WBGene00014027 . [P30636-1 ]
    GeneIDi 176258.
    KEGGi cel:CELE_ZK637.9.
    UCSCi ZK637.9b. c. elegans. [P30636-1 ]

    Organism-specific databases

    CTDi 176258.
    WormBasei ZK637.9a ; CE37946 ; WBGene00014027 ; tpk-1.
    ZK637.9b ; CE37947 ; WBGene00014027 ; tpk-1.

    Phylogenomic databases

    eggNOGi COG1564.
    GeneTreei ENSGT00390000016016.
    HOGENOMi HOG000018120.
    InParanoidi P30636.
    KOi K00949.
    OMAi WEFENIV.
    OrthoDBi EOG76HQ2J.
    PhylomeDBi P30636.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00597 .

    Miscellaneous databases

    NextBioi 891808.
    PROi P30636.

    Family and domain databases

    Gene3Di 2.60.120.320. 1 hit.
    3.40.50.10240. 1 hit.
    InterProi IPR006282. Thi_PPkinase.
    IPR016966. Thiamin_pyrophosphokinase_euk.
    IPR007373. Thiamin_PyroPKinase_B1-bd.
    IPR007371. TPK_catalytic.
    [Graphical view ]
    Pfami PF04265. TPK_B1_binding. 1 hit.
    PF04263. TPK_catalytic. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
    SMARTi SM00983. TPK_B1_binding. 1 hit.
    [Graphical view ]
    SUPFAMi SSF63862. SSF63862. 1 hit.
    SSF63999. SSF63999. 1 hit.
    TIGRFAMsi TIGR01378. thi_PPkinase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Thiamine pyrophosphate biosynthesis and transport in the nematode Caenorhabditis elegans."
      de Jong L., Meng Y., Dent J., Hekimi S.
      Genetics 168:845-854(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, MUTAGENESIS OF CYS-67.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiTPK1_CAEEL
    AccessioniPrimary (citable) accession number: P30636
    Secondary accession number(s): Q5FC86, Q6R5A6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 94 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3