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P30636 (TPK1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thiamin pyrophosphokinase 1

EC=2.7.6.2
Alternative name(s):
Thiamine pyrophosphokinase 1
Short name=TPK1
Gene names
Name:tpk-1
ORF Names:ZK637.9
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Functions cell non-autonomously. Ref.1

Catalytic activity

ATP + thiamine = AMP + thiamine diphosphate.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.

Sequence similarities

Belongs to the thiamine pyrophosphokinase family.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform b (identifier: P30636-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform a (identifier: P30636-2)

The sequence of this isoform differs from the canonical sequence as follows:
     146-160: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243Thiamin pyrophosphokinase 1
PRO_0000072646

Natural variations

Alternative sequence146 – 16015Missing in isoform a.
VSP_013462

Experimental info

Mutagenesis671C → Y in qm162; maternal-effect mutation. Partial loss-of-function. Slowdown and deregulation of physiological rhythms and lengthening of lifespan. No effect on oxygen consumption rate. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform b [UniParc].

Last modified April 26, 2005. Version 3.
Checksum: 2BA5401B76326678

FASTA24327,314
        10         20         30         40         50         60 
MSKKLKPFEI LEDSCASVCI WLNGEPTAIS NRAENLWNKA KYRVATDGAV NEILKRKSFV 

        70         80         90        100        110        120 
EWPHIICGDF DSINKQIDTK NAKVVHLPDQ DYTDLSKSVQ WCLEQKTLTS WEFENIVVLG 

       130        140        150        160        170        180 
GLNGRFDHTM STLSSLIRFV DSQTPVIVLD SRNLVLAVPT GDSNLDVNLE MTTKMCGIIP 

       190        200        210        220        230        240 
IVQKETIVSS IGLKYEMENL ALEFGKLIST SNEVTTSQVF LKSSSSLIFS IELENWVYKL 


DSL 

« Hide

Isoform a [UniParc].

Checksum: 78E3536535C2CA6C
Show »

FASTA22825,723

References

« Hide 'large scale' references
[1]"Thiamine pyrophosphate biosynthesis and transport in the nematode Caenorhabditis elegans."
de Jong L., Meng Y., Dent J., Hekimi S.
Genetics 168:845-854(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, MUTAGENESIS OF CYS-67.
[2]"The C. elegans genome sequencing project: a beginning."
Sulston J., Du Z., Thomas K., Wilson R., Hillier L., Staden R., Halloran N., Green P., Thierry-Mieg J., Qiu L., Dear S., Coulson A., Craxton M., Durbin R., Berks M., Metzstein M., Hawkins T., Ainscough R., Waterston R.
Nature 356:37-41(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[3]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY513235 mRNA. Translation: AAS21678.1.
Z11115 Genomic DNA. Translation: CAA77455.3.
Z11115 Genomic DNA. Translation: CAI46594.1.
PIRS15797.
RefSeqNP_001023023.1. NM_001027852.1. [P30636-2]
NP_001023024.1. NM_001027853.1. [P30636-1]
UniGeneCel.9539.

3D structure databases

ProteinModelPortalP30636.
SMRP30636. Positions 19-219.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaZK637.9b; ZK637.9b; WBGene00014027. [P30636-1]
GeneID176258.
KEGGcel:CELE_ZK637.9.
UCSCZK637.9b. c. elegans. [P30636-1]

Organism-specific databases

CTD176258.
WormBaseZK637.9a; CE37946; WBGene00014027; tpk-1.
ZK637.9b; CE37947; WBGene00014027; tpk-1.

Phylogenomic databases

eggNOGCOG1564.
GeneTreeENSGT00390000016016.
HOGENOMHOG000018120.
InParanoidP30636.
KOK00949.
OMAWEFENIV.
OrthoDBEOG76HQ2J.
PhylomeDBP30636.

Enzyme and pathway databases

UniPathwayUPA00060; UER00597.

Family and domain databases

Gene3D2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
InterProIPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsTIGR01378. thi_PPkinase. 1 hit.
ProtoNetSearch...

Other

NextBio891808.
PROP30636.

Entry information

Entry nameTPK1_CAEEL
AccessionPrimary (citable) accession number: P30636
Secondary accession number(s): Q5FC86, Q6R5A6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 26, 2005
Last modified: June 11, 2014
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase