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P30635 (GSHR_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glutathione reductase 2

Short name=GR
Short name=GRase
EC=1.8.1.7
Gene names
Name:trxr-2
ORF Names:ZK637.10
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Maintains high levels of reduced glutathione in the cytosol.

Catalytic activity

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactor

Binds 1 FAD per subunit By similarity.

Subcellular location

Cytoplasm By similarity.

Miscellaneous

The active site is a redox-active disulfide bond.

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Probable glutathione reductase 2
PRO_0000067958

Regions

Nucleotide binding58 – 6710FAD By similarity

Sites

Active site4761Proton acceptor By similarity

Amino acid modifications

Disulfide bond67 ↔ 72Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
P30635 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: CE2385C9ACBD9AD2

FASTA50355,046
        10         20         30         40         50         60 
MLLSTFKRHL PIRRLFSSNK FDLIVIGAGS GGLSCSKRAA DLGANVALID AVEPTPHGHS 

        70         80         90        100        110        120 
WGIGGTCANV GCIPKKLMHQ AAIVGKELKH ADKYGWNGID QEKIKHDWNV LSKNVNDRVK 

       130        140        150        160        170        180 
ANNWIYRVQL NQKKINYFNA YAEFVDKDKI VITGTDKNKT KNFLSAPNVV ISTGLRPKYP 

       190        200        210        220        230        240 
NIPGAELGIT SDDLFTLASV PGKTLIVGGG YVALECAGFL SAFNQNVEVL VRSIPLKGFD 

       250        260        270        280        290        300 
RDCVHFVMEH LKTTGVKVKE HVEVERVEAV GSKKKVTFTG NGGVEEYDTV IWAAGRVPNL 

       310        320        330        340        350        360 
KSLNLDNAGV RTDKRSGKIL ADEFDRASCN GVYAVGDIVQ DRQELTPLAI QSGKLLADRL 

       370        380        390        400        410        420 
FSNSKQIVRF DGVATTVFTP LELSTVGLTE EEAIQKHGED SIEVFHSHFT PFEYVVPQNK 

       430        440        450        460        470        480 
DSGFCYVKAV CTRDESQKIL GLHFVGPNAA EVIQGYAVAF RVGISMSDLQ NTIAIHPCSS 

       490        500 
EEFVKLHITK RSGQDPRTQG CCG 

« Hide

References

[1]"The C. elegans genome sequencing project: a beginning."
Sulston J., Du Z., Thomas K., Wilson R., Hillier L., Staden R., Halloran N., Green P., Thierry-Mieg J., Qiu L., Dear S., Coulson A., Craxton M., Durbin R., Berks M., Metzstein M., Hawkins T., Ainscough R., Waterston R.
Nature 356:37-41(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11115 Genomic DNA. Translation: CAA77459.1.
PIRD88542.
S15798.
RefSeqNP_498971.1. NM_066570.4.
UniGeneCel.9538.

3D structure databases

ProteinModelPortalP30635.
SMRP30635. Positions 18-496.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6239.ZK637.10.

Proteomic databases

PaxDbP30635.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaZK637.10.1; ZK637.10.1; WBGene00014028.
ZK637.10.2; ZK637.10.2; WBGene00014028.
GeneID176259.
KEGGcel:CELE_ZK637.10.
UCSCZK637.10. c. elegans.

Organism-specific databases

CTD40475.
WormBaseZK637.10; CE15373; WBGene00014028; trxr-2.

Phylogenomic databases

eggNOGCOG1249.
GeneTreeENSGT00390000007578.
HOGENOMHOG000276712.
InParanoidP30635.
KOK00383.
OMAGLSCSKR.
PhylomeDBP30635.

Family and domain databases

Gene3D3.30.390.30. 1 hit.
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
IPR006338. Thioredoxin/glutathione_Rdtase.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. SSF55424. 1 hit.
TIGRFAMsTIGR01438. TGR. 1 hit.
PROSITEPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio891814.
PROP30635.

Entry information

Entry nameGSHR_CAEEL
AccessionPrimary (citable) accession number: P30635
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase