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P30626 (SORCN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 149. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sorcin
Alternative name(s):
22 kDa protein
CP-22
Short name=CP22
V19
Gene names
Name:SRI
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length198 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Calcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels. Ref.13

Subunit structure

Homodimer. Interacts with GCA, RYR2 and ANXA7. Ref.10 Ref.13

Subcellular location

Cytoplasm. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note: Relocates to the sarcoplasmic reticulum membrane in response to elevated calcium levels.

Tissue specificity

Detected in cardiac myocytes.

Miscellaneous

This protein is encoded by an amplified gene in multidrug-resistant cells.

This protein has been shown to bind calcium with high affinity.

Sequence similarities

Contains 4 EF-hand domains.

Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
Sarcoplasmic reticulum
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandCalcium
Metal-binding
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processaction potential

Traceable author statement PubMed 9668070. Source: ProtInc

calcium ion transport

Inferred from electronic annotation. Source: Ensembl

cytoplasmic sequestering of transcription factor

Inferred from electronic annotation. Source: Ensembl

heart development

Traceable author statement PubMed 9668070. Source: ProtInc

intracellular sequestering of iron ion

Traceable author statement PubMed 9668070. Source: ProtInc

muscle organ development

Traceable author statement PubMed 9668070. Source: ProtInc

negative regulation of heart rate

Inferred from mutant phenotype PubMed 12754254. Source: BHF-UCL

negative regulation of ryanodine-sensitive calcium-release channel activity

Inferred from direct assay Ref.13. Source: BHF-UCL

negative regulation of transcription regulatory region DNA binding

Inferred from electronic annotation. Source: Ensembl

positive regulation of insulin secretion involved in cellular response to glucose stimulus

Inferred from electronic annotation. Source: Ensembl

positive regulation of release of sequestered calcium ion into cytosol

Inferred from electronic annotation. Source: Ensembl

regulation of calcium ion transport

Inferred from mutant phenotype PubMed 12754254PubMed 17130302. Source: BHF-UCL

regulation of cardiac muscle cell contraction

Inferred from mutant phenotype PubMed 12754254PubMed 17130302. Source: BHF-UCL

regulation of cell communication by electrical coupling

Traceable author statement Ref.13. Source: BHF-UCL

regulation of cell communication by electrical coupling involved in cardiac conduction

Inferred from mutant phenotype PubMed 17130302. Source: BHF-UCL

regulation of heart contraction

Traceable author statement PubMed 9668070. Source: ProtInc

regulation of high voltage-gated calcium channel activity

Inferred from mutant phenotype PubMed 12754254. Source: BHF-UCL

regulation of relaxation of muscle

Inferred from mutant phenotype PubMed 12754254. Source: BHF-UCL

regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum

Traceable author statement Ref.13. Source: BHF-UCL

regulation of striated muscle contraction

Traceable author statement PubMed 9668070. Source: ProtInc

signal transduction

Traceable author statement PubMed 16130169. Source: UniProtKB

transport

Traceable author statement PubMed 9668070. Source: ProtInc

   Cellular_componentT-tubule

Traceable author statement Ref.13. Source: BHF-UCL

Z disc

Inferred from direct assay PubMed 12754254. Source: BHF-UCL

cytoplasm

Traceable author statement PubMed 16130169. Source: UniProtKB

cytosol

Inferred from direct assay PubMed 10748169. Source: BHF-UCL

endoplasmic reticulum membrane

Inferred from direct assay PubMed 10672515. Source: BHF-UCL

extracellular vesicular exosome

Inferred from direct assay PubMed 15326289PubMed 19056867PubMed 20458337. Source: UniProt

membrane

Inferred from direct assay PubMed 10748169PubMed 17130302. Source: BHF-UCL

sarcoplasmic reticulum

Traceable author statement Ref.13. Source: BHF-UCL

sarcoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncalcium channel regulator activity

Traceable author statement PubMed 9668070. Source: ProtInc

calcium ion binding

Inferred from direct assay PubMed 17215369. Source: BHF-UCL

ion channel binding

Traceable author statement PubMed 12754254. Source: BHF-UCL

protease binding

Inferred from physical interaction PubMed 10748169. Source: BHF-UCL

protein heterodimerization activity

Inferred from physical interaction Ref.10. Source: BHF-UCL

receptor binding

Traceable author statement PubMed 9668070. Source: ProtInc

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

STAT3P407632EBI-750459,EBI-518675

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P30626-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P30626-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MAYPGHPGAGGGYYPGG → MQ
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 198198Sorcin
PRO_0000073725

Regions

Domain29 – 6436EF-hand 1
Domain70 – 10334EF-hand 2
Domain100 – 13536EF-hand 3
Domain134 – 16936EF-hand 4
Calcium binding83 – 94121 Probable
Calcium binding113 – 124122 Probable

Natural variations

Alternative sequence1 – 1717MAYPG…YYPGG → MQ in isoform 2.
VSP_046277

Experimental info

Mutagenesis1121F → L: Reduces affinity for calcium 5-fold.

Secondary structure

............................ 198
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: A4829C7650A5E3FA

FASTA19821,676
        10         20         30         40         50         60 
MAYPGHPGAG GGYYPGGYGG APGGPAFPGQ TQDPLYGYFA AVAGQDGQID ADELQRCLTQ 

        70         80         90        100        110        120 
SGIAGGYKPF NLETCRLMVS MLDRDMSGTM GFNEFKELWA VLNGWRQHFI SFDTDRSGTV 

       130        140        150        160        170        180 
DPQELQKALT TMGFRLSPQA VNSIAKRYST NGKITFDDYI ACCVKLRALT DSFRRRDTAQ 

       190 
QGVVNFPYDD FIQCVMSV 

« Hide

Isoform 2 [UniParc].

Checksum: A6B23B49FF8B9778
Show »

FASTA18320,345

References

« Hide 'large scale' references
[1]"Isolation and molecular cloning of human sorcin a calcium-binding protein in vincristine-resistant HOB1 lymphoma cells."
Wang S.L., Tam M.F., Ho Y.S., Pai S.H., Kao M.C.
Biochim. Biophys. Acta 1260:285-293(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Purification, cDNA cloning, and expression of human sorcin in vincristine-resistant HOB1 lymphoma cell lines."
Lee W.P.
Arch. Biochem. Biophys. 325:217-226(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Transcriptome analysis of human gastric cancer."
Oh J.H., Yang J.O., Hahn Y., Kim M.R., Byun S.S., Jeon Y.J., Kim J.M., Song K.S., Noh S.M., Kim S., Yoo H.S., Kim Y.S., Kim N.S.
Mamm. Genome 16:942-954(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Ascites.
[4]"Isolation of the cDNA clone for human CP22 overexpressed in multidrug-resistant cell lines."
Sugimoto Y., Asami N., Okochi E., Ogura M., Tsuruo T.
Submitted (MAR-1990) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[8]Lubec G., Chen W.-Q., Sun Y.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 57-116; 128-135 AND 154-165, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Fetal brain cortex.
[9]"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes."
Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J.
Electrophoresis 13:960-969(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 128-135 AND 154-165.
Tissue: Keratinocyte.
[10]"The PEF family proteins sorcin and grancalcin interact in vivo and in vitro."
Hansen C., Tarabykina S., la Cour J.M., Lollike K., Berchtold M.W.
FEBS Lett. 545:151-154(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GCA.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Crystal structure of calcium-free human sorcin: a member of the penta-EF-hand protein family."
Xie X., Dwyer M.D., Swenson L., Parker M.H., Botfield M.C.
Protein Sci. 10:2419-2425(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[13]"Molecular basis for the impaired function of the natural F112L sorcin mutant: X-ray crystal structure, calcium affinity, and interaction with annexin VII and the ryanodine receptor."
Franceschini S., Ilari A., Verzili D., Zamparelli C., Antaramian A., Rueda A., Valdivia H.H., Chiancone E., Colotti G.
FASEB J. 22:295-306(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF MUTANT LEU-112, SUBUNIT, FUNCTION, CALCIUM-BINDING, INTERACTION WITH RYR2 AND ANXA7.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L12387 mRNA. Translation: AAA92155.1.
BM739144 mRNA. No translation available.
M32886 mRNA. Translation: AAA60588.1.
AC003991 Genomic DNA. No translation available.
AC005075 Genomic DNA. No translation available.
CH471091 Genomic DNA. Translation: EAW76909.1.
CH471091 Genomic DNA. Translation: EAW76910.1.
BC011025 mRNA. Translation: AAH11025.1.
PIRS52094.
RefSeqNP_003121.1. NM_003130.3.
NP_944490.1. NM_198901.1.
UniGeneHs.489040.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JUOX-ray2.20A/B1-198[»]
2JC2X-ray2.50A/B/C/D1-198[»]
ProteinModelPortalP30626.
SMRP30626. Positions 27-198.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112595. 20 interactions.
IntActP30626. 10 interactions.
MINTMINT-4999795.
STRING9606.ENSP00000265729.

PTM databases

PhosphoSiteP30626.

Polymorphism databases

DMDM267021.

2D gel databases

OGPP30626.

Proteomic databases

PaxDbP30626.
PRIDEP30626.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000265729; ENSP00000265729; ENSG00000075142. [P30626-1]
ENST00000394641; ENSP00000378137; ENSG00000075142. [P30626-2]
GeneID6717.
KEGGhsa:6717.
UCSCuc003ujq.2. human. [P30626-1]

Organism-specific databases

CTD6717.
GeneCardsGC07M087834.
HGNCHGNC:11292. SRI.
HPAHPA019004.
MIM182520. gene.
neXtProtNX_P30626.
PharmGKBPA36117.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG298587.
HOGENOMHOG000231982.
HOVERGENHBG004492.
OMAGQQGVVN.
OrthoDBEOG7RV9FM.
PhylomeDBP30626.
TreeFamTF314682.

Gene expression databases

ArrayExpressP30626.
BgeeP30626.
CleanExHS_SRI.
GenevestigatorP30626.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 2 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSRI. human.
EvolutionaryTraceP30626.
GeneWikiSRI_(gene).
GenomeRNAi6717.
NextBio26200.
PROP30626.
SOURCESearch...

Entry information

Entry nameSORCN_HUMAN
AccessionPrimary (citable) accession number: P30626
Secondary accession number(s): A8MTH6, D6W5Q0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM