P30626 (SORCN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sorcin

Alternative name(s):
22 kDa protein
CP-22
Short name=CP22
V19

Gene names

Name:

SRI

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels. Ref.13
Subunit structure	Homodimer. Interacts with GCA, RYR2 and ANXA7. Ref.10 Ref.13
Subcellular location	Cytoplasm. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note: Relocates to the sarcoplasmic reticulum membrane in response to elevated calcium levels.
Tissue specificity	Detected in cardiac myocytes.
Miscellaneous	This protein is encoded by an amplified gene in multidrug-resistant cells. This protein has been shown to bind calcium with high affinity.
Sequence similarities	Contains 4 EF-hand domains.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane Sarcoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Calcium Metal-binding
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	calcium ion transport Inferred from electronic annotation. Source: Compara heart development Traceable author statement PubMed 9668070. Source: ProtInc intracellular sequestering of iron ion Traceable author statement PubMed 9668070. Source: ProtInc muscle organ development Traceable author statement PubMed 9668070. Source: ProtInc negative regulation of heart rate Inferred from mutant phenotype PubMed 12754254. Source: BHF-UCL negative regulation of ryanodine-sensitive calcium-release channel activity Inferred from direct assay Ref.13. Source: BHF-UCL regulation of action potential Traceable author statement PubMed 9668070. Source: ProtInc regulation of cardiac muscle cell contraction Inferred from mutant phenotype PubMed 12754254PubMed 17130302. Source: BHF-UCL regulation of cell communication by electrical coupling involved in cardiac conduction Inferred from mutant phenotype PubMed 17130302. Source: BHF-UCL regulation of relaxation of muscle Inferred from mutant phenotype PubMed 12754254. Source: BHF-UCL regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Traceable author statement Ref.13. Source: BHF-UCL regulation of voltage-gated calcium channel activity Inferred from mutant phenotype PubMed 12754254. Source: BHF-UCL signal transduction Traceable author statement PubMed 16130169. Source: UniProtKB transport Traceable author statement PubMed 9668070. Source: ProtInc
Cellular_component	T-tubule Traceable author statement Ref.13. Source: BHF-UCL Z disc Inferred from direct assay PubMed 12754254. Source: BHF-UCL cytosol Inferred from direct assay PubMed 10748169. Source: BHF-UCL endoplasmic reticulum membrane Inferred from direct assay PubMed 10672515. Source: BHF-UCL sarcoplasmic reticulum Traceable author statement Ref.13. Source: BHF-UCL sarcoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vesicle Inferred from electronic annotation. Source: Compara
Molecular_function	calcium channel regulator activity Traceable author statement PubMed 9668070. Source: ProtInc calcium ion binding Inferred from direct assay PubMed 17215369. Source: BHF-UCL ion channel binding Traceable author statement PubMed 12754254. Source: BHF-UCL receptor binding Traceable author statement PubMed 9668070. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
STAT3	P40763	2	EBI-750459,EBI-518675

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P30626-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P30626-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MAYPGHPGAGGGYYPGG → MQ

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 198

198

Sorcin

PRO_0000073725

Regions

Domain

29 – 64

36

EF-hand 1

Domain

70 – 103

34

EF-hand 2

Domain

100 – 135

36

EF-hand 3

Domain

134 – 169

36

EF-hand 4

Calcium binding

83 – 94

12

1 Probable

Calcium binding

113 – 124

12

2 Probable

Natural variations

Alternative sequence

1 – 17

17

MAYPG…YYPGG → MQ in isoform 2.

VSP_046277

Experimental info

Mutagenesis

112

1

F → L: Reduces affinity for calcium 5-fold.

Secondary structure

1

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198

Helix Strand Turn

Details...

Helix

36 – 43

8

Turn

44 – 47

4

Helix

51 – 61

11

Turn

62 – 64

3

Beta strand

65 – 68

4

Helix

72 – 82

11

Beta strand

84 – 86

3

Helix

92 – 110

19

Beta strand

118 – 120

3

Helix

122 – 131

10

Helix

138 – 147

10

Beta strand

150 – 155

6

Helix

156 – 175

20

Helix

178 – 180

3

Beta strand

182 – 187

6

Helix

188 – 195

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: A4829C7650A5E3FA	FASTA	198	21,676
	10 20 30 40 50 60 MAYPGHPGAG GGYYPGGYGG APGGPAFPGQ TQDPLYGYFA AVAGQDGQID ADELQRCLTQ 70 80 90 100 110 120 SGIAGGYKPF NLETCRLMVS MLDRDMSGTM GFNEFKELWA VLNGWRQHFI SFDTDRSGTV 130 140 150 160 170 180 DPQELQKALT TMGFRLSPQA VNSIAKRYST NGKITFDDYI ACCVKLRALT DSFRRRDTAQ 190 QGVVNFPYDD FIQCVMSV
	Isoform 2 [UniParc]. Checksum: A6B23B49FF8B9778	FASTA	183	20,345
	10 20 30 40 50 60 MQYGGAPGGP AFPGQTQDPL YGYFAAVAGQ DGQIDADELQ RCLTQSGIAG GYKPFNLETC 70 80 90 100 110 120 RLMVSMLDRD MSGTMGFNEF KELWAVLNGW RQHFISFDTD RSGTVDPQEL QKALTTMGFR 130 140 150 160 170 180 LSPQAVNSIA KRYSTNGKIT FDDYIACCVK LRALTDSFRR RDTAQQGVVN FPYDDFIQCV MSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and molecular cloning of human sorcin a calcium-binding protein in vincristine-resistant HOB1 lymphoma cells." Wang S.L., Tam M.F., Ho Y.S., Pai S.H., Kao M.C. Biochim. Biophys. Acta 1260:285-293(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Purification, cDNA cloning, and expression of human sorcin in vincristine-resistant HOB1 lymphoma cell lines." Lee W.P. Arch. Biochem. Biophys. 325:217-226(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Transcriptome analysis of human gastric cancer." Oh J.H., Yang J.O., Hahn Y., Kim M.R., Byun S.S., Jeon Y.J., Kim J.M., Song K.S., Noh S.M., Kim S., Yoo H.S., Kim Y.S., Kim N.S. Mamm. Genome 16:942-954(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Ascites.
[4]	"Isolation of the cDNA clone for human CP22 overexpressed in multidrug-resistant cell lines." Sugimoto Y., Asami N., Okochi E., Ogura M., Tsuruo T. Submitted (MAR-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[8]	Lubec G., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 57-116; 128-135 AND 154-165, MASS SPECTROMETRY. Tissue: Fetal brain cortex.
[9]	"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes." Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J. Electrophoresis 13:960-969(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 128-135 AND 154-165. Tissue: Keratinocyte.
[10]	"The PEF family proteins sorcin and grancalcin interact in vivo and in vitro." Hansen C., Tarabykina S., la Cour J.M., Lollike K., Berchtold M.W. FEBS Lett. 545:151-154(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GCA.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"Crystal structure of calcium-free human sorcin: a member of the penta-EF-hand protein family." Xie X., Dwyer M.D., Swenson L., Parker M.H., Botfield M.C. Protein Sci. 10:2419-2425(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[13]	"Molecular basis for the impaired function of the natural F112L sorcin mutant: X-ray crystal structure, calcium affinity, and interaction with annexin VII and the ryanodine receptor." Franceschini S., Ilari A., Verzili D., Zamparelli C., Antaramian A., Rueda A., Valdivia H.H., Chiancone E., Colotti G. FASEB J. 22:295-306(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF MUTANT LEU-112, SUBUNIT, FUNCTION, CALCIUM-BINDING, INTERACTION WITH RYR2 AND ANXA7.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L12387 mRNA. Translation: AAA92155.1.
BM739144 mRNA. No translation available.
M32886 mRNA. Translation: AAA60588.1.
AC003991 Genomic DNA. No translation available.
AC005075 Genomic DNA. No translation available.
CH471091 Genomic DNA. Translation: EAW76909.1.
CH471091 Genomic DNA. Translation: EAW76910.1.
BC011025 mRNA. Translation: AAH11025.1.

IPI

IPI00027175.
IPI01013070.

PIR

S52094.

RefSeq

NP_003121.1. NM_003130.3.
NP_944490.1. NM_198901.1.

UniGene

Hs.489040.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JUO	X-ray	2.20	A/B	1-198	[»]
2JC2	X-ray	2.50	A/B/C/D	1-198	[»]

ProteinModelPortal

P30626.

ModBase

Search...

Protein-protein interaction databases

IntAct

P30626. 6 interactions.

MINT

MINT-4999795.

STRING

9606.ENSP00000265729.

PTM databases

PhosphoSite

P30626.

Polymorphism databases

DMDM

267021.

2D gel databases

OGP

P30626.

Proteomic databases

PaxDb

P30626.

PRIDE

P30626.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000265729; ENSP00000265729; ENSG00000075142.
ENST00000394641; ENSP00000378137; ENSG00000075142.

GeneID

6717.

KEGG

hsa:6717.

UCSC

uc003ujq.1. human.

Organism-specific databases

CTD

6717.

GeneCards

GC07M087834.

HGNC

HGNC:11292. SRI.

MIM

182520. gene.

neXtProt

NX_P30626.

PharmGKB

PA36117.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG298587.

HOGENOM

HOG000231982.

HOVERGEN

HBG004492.

OMA

RRDSGQQ.

OrthoDB

EOG4ZS949.

PhylomeDB

P30626.

Gene expression databases

ArrayExpress

P30626.

Bgee

P30626.

CleanEx

HS_SRI.

Genevestigator

P30626.

GermOnline

ENSG00000075142. Homo sapiens.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]

Pfam

PF13499. EF_hand_5. 1 hit.
[Graphical view]

SMART

SM00054. EFh. 2 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

SRI. human.

EvolutionaryTrace

P30626.

GenomeRNAi

6717.

NextBio

26200.

SOURCE

Search...

Entry information

Entry name

SORCN_HUMAN

Accession

Primary (citable) accession number: P30626
Secondary accession number(s): A8MTH6, D6W5Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 139 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.