Reviewed, UniProtKB/Swiss-Prot P30625 (KAPR_CAEEL)

Names and origin

Protein names

Recommended name:
    cAMP-dependent protein kinase regulatory subunit

Gene names

Name:	kin-2
Synonyms:	kin-a
ORF Names:	R07E4.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer that binds four cAMP molecules.
Subcellular location	Cytoplasm › cytosol. Ref.1
Developmental stage	Expressed at a low level during embryogenesis. Expression increases sharply approximately four hours before hatching with levels peaking during the first larval stage. Levels then decrease as development progresses to adulthood. Ref.1
Post-translational modification	The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain but is not phosphorylated. The physiological significance of phosphorylations by other kinases is unclear.
Sequence similarities	Belongs to the cAMP-dependent kinase regulatory chain family. Contains 2 cyclic nucleotide-binding domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Nucleotide-binding cAMP cAMP-binding
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cAMP-dependent protein kinase complex Inferred from electronic annotation. Source: InterPro cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cAMP binding Inferred from electronic annotation. Source: UniProtKB-KW cAMP-dependent protein kinase regulator activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q17964	1	EBI-325716,EBI-325726
	Q20177	1	EBI-325716,EBI-325747
	Q8MQD2	1	EBI-325716,EBI-313681
	Q95XK1	1	EBI-325716,EBI-325771
	Q95XK2	1	EBI-325716,EBI-325775
	Q9U1U3	1	EBI-325716,EBI-325789
kbp-5	O44956	1	EBI-325716,EBI-325730
nhr-48	Q94407	1	EBI-325716,EBI-325793
sodh-1	Q17334	1	EBI-325716,EBI-313947

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P30625-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: P30625-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: MSGGNEEDQL...FEKLNEQRAQ → MGQQLSNRRNSQSVGATKNAKTPKPK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	cAMP-dependent protein kinase regulatory subunit		PRO_0000205394
Regions
	Nucleotide binding	122 – 239	118	cAMP 1
	Nucleotide binding	240 – 366	127	cAMP 2
	Region	1 – 121	121	Dimerization and phosphorylation
	Motif	82 – 86	5	Pseudophosphorylation motif
Sites
	Binding site	187	1	cAMP 1
	Binding site	196	1	cAMP 1
	Binding site	311	1	cAMP 2
	Binding site	320	1	cAMP 2
Amino acid modifications
	Modified residue	87	1	Phosphoserine By similarity
	Disulfide bond	13		Interchain (with C-34) By similarity
	Disulfide bond	34		Interchain (with C-13) By similarity
Natural variations
	Alternative sequence	1 – 58	58	MSGGN…EQRAQ → MGQQLSNRRNSQSVGATKNA KTPKPK in isoform b.		VSP_015952
Experimental info
	Sequence conflict	204	1	T → I in AAA27980. Ref.1
	Sequence conflict	250	1	A → V in AAA27980. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified May 16, 2006. Version 3. Checksum: 8EE6AF7111A58C0E	FASTA	366	41,468
	10 20 30 40 50 60 MSGGNEEDQL AQCQAYVQRH NIQQLVKEAI VVLCIHKPDN PVLFLKDHFE KLNEQRAQEG 70 80 90 100 110 120 GNPDAADDDD IIVEPPKRSG GRRTGISAEP IKEDDTEYKK VVIPKDDATR RSLESAMRKN 130 140 150 160 170 180 LLFAHLEEDE QKTMYDAMFP VEKSAGETII EQGEEGDNFY VIDKGTVDVY VNHEYVLTIN 190 200 210 220 230 240 EGGSFGELAL IYGTPRAATV IAKTDVKLWA IDRLTYRRIL MGSVTKKRKM YDEFLSKVQI 250 260 270 280 290 300 LADLDQWERA NVADALERCD FEPGTHVVEQ GQPGDEFFII LEGEANVLQK RSDDAPFDVV 310 320 330 340 350 360 GHLGMSDYFG EIALLLDRPR AATVVAKTHL KCIKLDRNRF ERVMGPVREI LKRDVSNYNS YVKLMT
	Isoform b. Checksum: 94AFC203F2AF2539	FASTA	334	37,580
	10 20 30 40 50 60 MGQQLSNRRN SQSVGATKNA KTPKPKEGGN PDAADDDDII VEPPKRSGGR RTGISAEPIK 70 80 90 100 110 120 EDDTEYKKVV IPKDDATRRS LESAMRKNLL FAHLEEDEQK TMYDAMFPVE KSAGETIIEQ 130 140 150 160 170 180 GEEGDNFYVI DKGTVDVYVN HEYVLTINEG GSFGELALIY GTPRAATVIA KTDVKLWAID 190 200 210 220 230 240 RLTYRRILMG SVTKKRKMYD EFLSKVQILA DLDQWERANV ADALERCDFE PGTHVVEQGQ 250 260 270 280 290 300 PGDEFFIILE GEANVLQKRS DDAPFDVVGH LGMSDYFGEI ALLLDRPRAA TVVAKTHLKC 310 320 330 IKLDRNRFER VMGPVREILK RDVSNYNSYV KLMT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, structure, and expression of the gene for a novel regulatory subunit of cAMP-dependent protein kinase in Caenorhabditis elegans." Lu X., Gross R.E., Bagchi S., Rubin C.S. J. Biol. Chem. 265:3293-3303(1990) [PubMed: 2303451] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J05220 mRNA. Translation: AAA27980.1. Different initiation. U39652 Genomic DNA. Translation: AAA80405.2. U39652 Genomic DNA. Translation: AAK82909.1.
PIR	OKKW1R. A35076. T16701.
RefSeq	NP_508999.2.
UniGene	Cel.5527
3D structure databases
HSSP	HSSP built from PDB template 1RGS based on UniProtKB P00514.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:26281N.
IntAct	P30625. 17 interactions.
Proteomic databases
PRIDE	P30625.
Genome annotation databases
Ensembl	R07E4.6. Caenorhabditis elegans. [Contig view]
GeneID	180864.
Organism-specific databases
WormBase	WBGene00002190. kin-2.
WormPep	R07E4.6a. CE39609. [WorfDB] R07E4.6b. CE28749. [WorfDB]
Gene expression databases
ArrayExpress	P30625.
Family and domain databases
InterPro	IPR003117. cAMP-dep_prot_kin_reg_I/II_a/b. IPR002373. cAMP/cGMP_kin. IPR000595. cNMP_bd. IPR018488. cNMP_bd_CS. IPR012198. PK_regulatory. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF00027. cNMP_binding. 2 hits. PF02197. RIIa. 1 hit. [Graphical view]
PIRSF	PIRSF000548. PK_regulatory. 1 hit.
PRINTS	PR00103. CAMPKINASE.
SMART	SM00100. cNMP. 2 hits. SM00394. RIIa. 1 hit. [Graphical view]
PROSITE	PS00888. CNMP_BINDING_1. 2 hits. PS00889. CNMP_BINDING_2. 2 hits. PS50042. CNMP_BINDING_3. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	911310.

Entry information

Entry name

KAPR_CAEEL

Accession

Primary (citable) accession number: P30625
Secondary accession number(s): Q21820, Q95ZR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project