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Protein

cAMP-dependent protein kinase regulatory subunit

Gene

kin-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Controls the rhythmic contraction of enteric muscles probably by regulating G-protein coupled receptor aex-2-mediated calcium influx in GABAergic DVB neurons.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei187 – 1871cAMP 1
Binding sitei196 – 1961cAMP 1
Binding sitei311 – 3111cAMP 2
Binding sitei320 – 3201cAMP 2

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi122 – 239118cAMP 1Add
BLAST
Nucleotide bindingi240 – 366127cAMP 2Add
BLAST

GO - Molecular functioni

  • cAMP binding Source: UniProtKB-KW
  • cAMP-dependent protein kinase regulator activity Source: WormBase

GO - Biological processi

  • medium-term memory Source: WormBase
  • negative regulation of oocyte maturation Source: WormBase
  • negative regulation of protein kinase C signaling Source: WormBase
  • regulation of establishment of cell polarity Source: WormBase
  • regulation of protein kinase activity Source: GOC
Complete GO annotation...

Keywords - Ligandi

cAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-163615. PKA activation.
R-CEL-164378. PKA activation in glucagon signalling.
R-CEL-180024. DARPP-32 events.
R-CEL-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-CEL-5610787. Hedgehog 'off' state.
R-CEL-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase regulatory subunit
Gene namesi
Name:kin-2
Synonyms:kin-a
ORF Names:R07E4.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiR07E4.6a; CE39609; WBGene00002190; kin-2.
R07E4.6b; CE28749; WBGene00002190; kin-2.
R07E4.6c; CE04821; WBGene00002190; kin-2.

Subcellular locationi

  • Cytoplasmcytosol 1 Publication

GO - Cellular componenti

  • cAMP-dependent protein kinase complex Source: WormBase
  • cytosol Source: WormBase
  • membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi310 – 3101G → D: Loss of cAMP binding which may prevent dissociation from kin-1. Contraction of enteric muscles is partially impaired. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366cAMP-dependent protein kinase regulatory subunitPRO_0000205394Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi13 – 13Interchain (with C-34)By similarity
Disulfide bondi34 – 34Interchain (with C-13)By similarity
Modified residuei87 – 871PhosphoserineBy similarity

Post-translational modificationi

The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain but is not phosphorylated. The physiological significance of phosphorylations by other kinases is unclear.

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP30625.
PaxDbiP30625.
PRIDEiP30625.

Expressioni

Developmental stagei

Expressed at a low level during embryogenesis. Expression increases sharply approximately four hours before hatching with levels peaking during the first larval stage. Levels then decrease as development progresses to adulthood.1 Publication

Gene expression databases

ExpressionAtlasiP30625. baseline and differential.

Interactioni

Subunit structurei

Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer that binds four cAMP molecules.

Protein-protein interaction databases

BioGridi45796. 17 interactions.
DIPiDIP-26281N.
IntActiP30625. 9 interactions.
MINTiMINT-1053467.
STRINGi6239.R07E4.6c.

Structurei

3D structure databases

ProteinModelPortaliP30625.
SMRiP30625. Positions 10-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 121121Dimerization and phosphorylationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi82 – 865Pseudophosphorylation motif

Sequence similaritiesi

Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1113. Eukaryota.
COG0664. LUCA.
GeneTreeiENSGT00530000062947.
HOGENOMiHOG000196669.
InParanoidiP30625.
KOiK04739.
OMAiDQWERAN.
OrthoDBiEOG72JWG6.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR012198. cAMP_dep_PK_reg_su.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF02197. RIIa. 1 hit.
[Graphical view]
PIRSFiPIRSF000548. PK_regulatory. 1 hit.
SMARTiSM00100. cNMP. 2 hits.
SM00394. RIIa. 1 hit.
[Graphical view]
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF51206. SSF51206. 2 hits.
PROSITEiPS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aImported (identifier: P30625-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGGNEEDQL AQCQAYVQRH NIQQLVKEAI VVLCIHKPDN PVLFLKDHFE
60 70 80 90 100
KLNEQRAQEG GNPDAADDDD IIVEPPKRSG GRRTGISAEP IKEDDTEYKK
110 120 130 140 150
VVIPKDDATR RSLESAMRKN LLFAHLEEDE QKTMYDAMFP VEKSAGETII
160 170 180 190 200
EQGEEGDNFY VIDKGTVDVY VNHEYVLTIN EGGSFGELAL IYGTPRAATV
210 220 230 240 250
IAKTDVKLWA IDRLTYRRIL MGSVTKKRKM YDEFLSKVQI LADLDQWERA
260 270 280 290 300
NVADALERCD FEPGTHVVEQ GQPGDEFFII LEGEANVLQK RSDDAPFDVV
310 320 330 340 350
GHLGMSDYFG EIALLLDRPR AATVVAKTHL KCIKLDRNRF ERVMGPVREI
360
LKRDVSNYNS YVKLMT
Length:366
Mass (Da):41,468
Last modified:May 16, 2006 - v3
Checksum:i8EE6AF7111A58C0E
GO
Isoform bImported (identifier: P30625-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: MSGGNEEDQL...FEKLNEQRAQ → MGQQLSNRRNSQSVGATKNAKTPKPK

Note: No experimental confirmation available.Curated
Show »
Length:334
Mass (Da):37,580
Checksum:i94AFC203F2AF2539
GO
Isoform cImported (identifier: P30625-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNNYSGDIVFM

Note: No experimental confirmation available.Curated
Show »
Length:376
Mass (Da):42,609
Checksum:iEE8E6747CFD794EF
GO

Sequence cautioni

The sequence AAA27980.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti204 – 2041T → I in AAA27980 (PubMed:2303451).Curated
Sequence conflicti250 – 2501A → V in AAA27980 (PubMed:2303451).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5858MSGGN…EQRAQ → MGQQLSNRRNSQSVGATKNA KTPKPK in isoform b. CuratedVSP_015952Add
BLAST
Alternative sequencei1 – 11M → MNNYSGDIVFM in isoform c. CuratedVSP_057512

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05220 mRNA. Translation: AAA27980.1. Different initiation.
FO080885 Genomic DNA. Translation: CCD67512.1.
FO080885 Genomic DNA. Translation: CCD67513.1.
FO080885 Genomic DNA. Translation: CCD67514.1.
PIRiA35076. OKKW1R.
T16701.
RefSeqiNP_001024842.1. NM_001029671.3. [P30625-2]
NP_001076771.1. NM_001083302.1. [P30625-3]
NP_508999.2. NM_076598.5. [P30625-1]
UniGeneiCel.5527.

Genome annotation databases

EnsemblMetazoaiR07E4.6a; R07E4.6a; WBGene00002190. [P30625-1]
GeneIDi180864.
KEGGicel:CELE_R07E4.6.
UCSCiR07E4.6a. c. elegans. [P30625-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05220 mRNA. Translation: AAA27980.1. Different initiation.
FO080885 Genomic DNA. Translation: CCD67512.1.
FO080885 Genomic DNA. Translation: CCD67513.1.
FO080885 Genomic DNA. Translation: CCD67514.1.
PIRiA35076. OKKW1R.
T16701.
RefSeqiNP_001024842.1. NM_001029671.3. [P30625-2]
NP_001076771.1. NM_001083302.1. [P30625-3]
NP_508999.2. NM_076598.5. [P30625-1]
UniGeneiCel.5527.

3D structure databases

ProteinModelPortaliP30625.
SMRiP30625. Positions 10-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi45796. 17 interactions.
DIPiDIP-26281N.
IntActiP30625. 9 interactions.
MINTiMINT-1053467.
STRINGi6239.R07E4.6c.

Proteomic databases

EPDiP30625.
PaxDbiP30625.
PRIDEiP30625.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR07E4.6a; R07E4.6a; WBGene00002190. [P30625-1]
GeneIDi180864.
KEGGicel:CELE_R07E4.6.
UCSCiR07E4.6a. c. elegans. [P30625-1]

Organism-specific databases

CTDi180864.
WormBaseiR07E4.6a; CE39609; WBGene00002190; kin-2.
R07E4.6b; CE28749; WBGene00002190; kin-2.
R07E4.6c; CE04821; WBGene00002190; kin-2.

Phylogenomic databases

eggNOGiKOG1113. Eukaryota.
COG0664. LUCA.
GeneTreeiENSGT00530000062947.
HOGENOMiHOG000196669.
InParanoidiP30625.
KOiK04739.
OMAiDQWERAN.
OrthoDBiEOG72JWG6.

Enzyme and pathway databases

ReactomeiR-CEL-163615. PKA activation.
R-CEL-164378. PKA activation in glucagon signalling.
R-CEL-180024. DARPP-32 events.
R-CEL-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-CEL-5610787. Hedgehog 'off' state.
R-CEL-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

NextBioi911310.
PROiP30625.

Gene expression databases

ExpressionAtlasiP30625. baseline and differential.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR012198. cAMP_dep_PK_reg_su.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF02197. RIIa. 1 hit.
[Graphical view]
PIRSFiPIRSF000548. PK_regulatory. 1 hit.
SMARTiSM00100. cNMP. 2 hits.
SM00394. RIIa. 1 hit.
[Graphical view]
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF51206. SSF51206. 2 hits.
PROSITEiPS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, structure, and expression of the gene for a novel regulatory subunit of cAMP-dependent protein kinase in Caenorhabditis elegans."
    Lu X., Gross R.E., Bagchi S., Rubin C.S.
    J. Biol. Chem. 265:3293-3303(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "PKA controls calcium influx into motor neurons during a rhythmic behavior."
    Wang H., Sieburth D.
    PLoS Genet. 9:E1003831-E1003831(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLY-310.

Entry informationi

Entry nameiKAPR_CAEEL
AccessioniPrimary (citable) accession number: P30625
Secondary accession number(s): Q0MQ68, Q21820, Q95ZR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: May 16, 2006
Last modified: May 11, 2016
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.