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P30624

- LCF1_YEAST

UniProt

P30624 - LCF1_YEAST

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Protein
Long-chain-fatty-acid--CoA ligase 1
Gene
FAA1, YOR317W, O6136
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It may supplement intracellular myristoyl-CoA pools from exogenous myristate. Preferentially acts on C12:0-C16:0 fatty acids with myristic and pentadecanic acid (C15:0) having the highest activities.

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Magnesium.

Temperature dependencei

Optimum temperature is 30 degrees Celsius.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: SGD
  3. medium-chain fatty acid-CoA ligase activity Source: SGD

GO - Biological processi

  1. long-chain fatty acid metabolic process Source: GOC
  2. long-chain fatty acid transport Source: SGD
  3. long-chain fatty-acyl-CoA metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YOR317W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 1 (EC:6.2.1.3)
Alternative name(s):
Fatty acid activator 1
Long-chain acyl-CoA synthetase 1
Gene namesi
Name:FAA1
Ordered Locus Names:YOR317W
ORF Names:O6136
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR317w.
SGDiS000005844. FAA1.

Subcellular locationi

GO - Cellular componenti

  1. lipid particle Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 700700Long-chain-fatty-acid--CoA ligase 1
PRO_0000193119Add
BLAST

Proteomic databases

MaxQBiP30624.
PaxDbiP30624.
PeptideAtlasiP30624.

Expressioni

Gene expression databases

GenevestigatoriP30624.

Interactioni

Protein-protein interaction databases

BioGridi34704. 68 interactions.
DIPiDIP-4654N.
IntActiP30624. 1 interaction.
MINTiMINT-541922.
STRINGi4932.YOR317W.

Structurei

3D structure databases

ProteinModelPortaliP30624.
SMRiP30624. Positions 112-604.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1022.
GeneTreeiENSGT00690000102168.
HOGENOMiHOG000159459.
KOiK01897.
OMAiTETCANT.
OrthoDBiEOG7CCC0K.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30624-1 [UniParc]FASTAAdd to Basket

« Hide

MVAQYTVPVG KAANEHETAP RRNYQCREKP LVRPPNTKCS TVYEFVLECF    50
QKNKNSNAMG WRDVKEIHEE SKSVMKKVDG KETSVEKKWM YYELSHYHYN 100
SFDQLTDIMH EIGRGLVKIG LKPNDDDKLH LYAATSHKWM KMFLGAQSQG 150
IPVVTAYDTL GEKGLIHSLV QTGSKAIFTD NSLLPSLIKP VQAAQDVKYI 200
IHFDSISSED RRQSGKIYQS AHDAINRIKE VRPDIKTFSF DDILKLGKES 250
CNEIDVHPPG KDDLCCIMYT SGSTGEPKGV VLKHSNVVAG VGGASLNVLK 300
FVGNTDRVIC FLPLAHIFEL VFELLSFYWG ACIGYATVKT LTSSSVRNCQ 350
GDLQEFKPTI MVGVAAVWET VRKGILNQID NLPFLTKKIF WTAYNTKLNM 400
QRLHIPGGGA LGNLVFKKIR TATGGQLRYL LNGGSPISRD AQEFITNLIC 450
PMLIGYGLTE TCASTTILDP ANFELGVAGD LTGCVTVKLV DVEELGYFAK 500
NNQGEVWITG ANVTPEYYKN EEETSQALTS DGWFKTGDIG EWEANGHLKI 550
IDRKKNLVKT MNGEYIALEK LESVYRSNEY VANICVYADQ SKTKPVGIIV 600
PNHAPLTKLA KKLGIMEQKD SSINIENYLE DAKLIKAVYS DLLKTGKDQG 650
LVGIELLAGI VFFDGEWTPQ NGFVTSAQKL KRKDILNAVK DKVDAVYSSS 700
Length:700
Mass (Da):77,866
Last modified:April 1, 1993 - v1
Checksum:i56EE7E04D95BC5C9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66194 Genomic DNA. Translation: CAA46957.1.
X90565 Genomic DNA. Translation: CAA62172.1.
Z75225 Genomic DNA. Translation: CAA99637.1.
BK006948 Genomic DNA. Translation: DAA11082.1.
PIRiS23052.
RefSeqiNP_014962.3. NM_001183737.3.

Genome annotation databases

EnsemblFungiiYOR317W; YOR317W; YOR317W.
GeneIDi854495.
KEGGisce:YOR317W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66194 Genomic DNA. Translation: CAA46957.1 .
X90565 Genomic DNA. Translation: CAA62172.1 .
Z75225 Genomic DNA. Translation: CAA99637.1 .
BK006948 Genomic DNA. Translation: DAA11082.1 .
PIRi S23052.
RefSeqi NP_014962.3. NM_001183737.3.

3D structure databases

ProteinModelPortali P30624.
SMRi P30624. Positions 112-604.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34704. 68 interactions.
DIPi DIP-4654N.
IntActi P30624. 1 interaction.
MINTi MINT-541922.
STRINGi 4932.YOR317W.

Proteomic databases

MaxQBi P30624.
PaxDbi P30624.
PeptideAtlasi P30624.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR317W ; YOR317W ; YOR317W .
GeneIDi 854495.
KEGGi sce:YOR317W.

Organism-specific databases

CYGDi YOR317w.
SGDi S000005844. FAA1.

Phylogenomic databases

eggNOGi COG1022.
GeneTreei ENSGT00690000102168.
HOGENOMi HOG000159459.
KOi K01897.
OMAi TETCANT.
OrthoDBi EOG7CCC0K.

Enzyme and pathway databases

BioCyci YEAST:YOR317W-MONOMER.

Miscellaneous databases

NextBioi 976828.

Gene expression databases

Genevestigatori P30624.

Family and domain databases

InterProi IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a Saccharomyces cerevisiae long chain fatty acyl:CoA synthetase gene (FAA1) and assessment of its role in protein N-myristoylation."
    Duronio R.J., Knoll L.J., Gordon J.I.
    J. Cell Biol. 117:515-529(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 4-14.
    Strain: ATCC 204508 / S288c.
  2. "Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome XV reveals regions of similarity to chromosomes I and XIII."
    Pearson B.M., Hernando Y., Payne J., Wolf S.S., Kalogeropoulos A., Schweizer M.
    Yeast 12:1021-1031(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Biochemical studies of three Saccharomyces cerevisiae acyl-CoA synthetases, Faa1p, Faa2p, and Faa3p."
    Knoll L.J., Johnson D.R., Gordon J.I.
    J. Biol. Chem. 269:16348-16356(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLCF1_YEAST
AccessioniPrimary (citable) accession number: P30624
Secondary accession number(s): D6W316
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: September 3, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7470 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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