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Protein

Long-chain-fatty-acid--CoA ligase 1

Gene

FAA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It may supplement intracellular myristoyl-CoA pools from exogenous myristate. Preferentially acts on C12:0-C16:0 fatty acids with myristic and pentadecanic acid (C15:0) having the highest activities.

Miscellaneous

Present with 7470 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Temperature dependencei

Optimum temperature is 30 degrees Celsius.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • decanoate-CoA ligase activity Source: UniProtKB-EC
  • long-chain fatty acid-CoA ligase activity Source: SGD
  • medium-chain fatty acid-CoA ligase activity Source: SGD

GO - Biological processi

  • long-chain fatty acid import Source: SGD
  • long-chain fatty-acyl-CoA metabolic process Source: SGD
  • sphingoid long-chain base transport Source: SGD

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YOR317W-MONOMER
ReactomeiR-SCE-75876 Synthesis of very long-chain fatty acyl-CoAs

Chemistry databases

SwissLipidsiSLP:000000132

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 1 (EC:6.2.1.3)
Alternative name(s):
Fatty acid activator 1
Long-chain acyl-CoA synthetase 1
Gene namesi
Name:FAA1
Ordered Locus Names:YOR317W
ORF Names:O6136
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR317W
SGDiS000005844 FAA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931191 – 700Long-chain-fatty-acid--CoA ligase 1Add BLAST700

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki189Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP30624
PaxDbiP30624
PRIDEiP30624

PTM databases

CarbonylDBiP30624
iPTMnetiP30624

Interactioni

Protein-protein interaction databases

BioGridi34704136 interactors.
DIPiDIP-4654N
IntActiP30624 9 interactors.
STRINGi4932.YOR317W

Structurei

3D structure databases

ProteinModelPortaliP30624
SMRiP30624
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00870000136474
HOGENOMiHOG000159459
InParanoidiP30624
KOiK01897
OMAiGPLVCCE
OrthoDBiEOG092C0RYI

Family and domain databases

InterProiView protein in InterPro
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

P30624-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAQYTVPVG KAANEHETAP RRNYQCREKP LVRPPNTKCS TVYEFVLECF
60 70 80 90 100
QKNKNSNAMG WRDVKEIHEE SKSVMKKVDG KETSVEKKWM YYELSHYHYN
110 120 130 140 150
SFDQLTDIMH EIGRGLVKIG LKPNDDDKLH LYAATSHKWM KMFLGAQSQG
160 170 180 190 200
IPVVTAYDTL GEKGLIHSLV QTGSKAIFTD NSLLPSLIKP VQAAQDVKYI
210 220 230 240 250
IHFDSISSED RRQSGKIYQS AHDAINRIKE VRPDIKTFSF DDILKLGKES
260 270 280 290 300
CNEIDVHPPG KDDLCCIMYT SGSTGEPKGV VLKHSNVVAG VGGASLNVLK
310 320 330 340 350
FVGNTDRVIC FLPLAHIFEL VFELLSFYWG ACIGYATVKT LTSSSVRNCQ
360 370 380 390 400
GDLQEFKPTI MVGVAAVWET VRKGILNQID NLPFLTKKIF WTAYNTKLNM
410 420 430 440 450
QRLHIPGGGA LGNLVFKKIR TATGGQLRYL LNGGSPISRD AQEFITNLIC
460 470 480 490 500
PMLIGYGLTE TCASTTILDP ANFELGVAGD LTGCVTVKLV DVEELGYFAK
510 520 530 540 550
NNQGEVWITG ANVTPEYYKN EEETSQALTS DGWFKTGDIG EWEANGHLKI
560 570 580 590 600
IDRKKNLVKT MNGEYIALEK LESVYRSNEY VANICVYADQ SKTKPVGIIV
610 620 630 640 650
PNHAPLTKLA KKLGIMEQKD SSINIENYLE DAKLIKAVYS DLLKTGKDQG
660 670 680 690 700
LVGIELLAGI VFFDGEWTPQ NGFVTSAQKL KRKDILNAVK DKVDAVYSSS
Length:700
Mass (Da):77,866
Last modified:April 1, 1993 - v1
Checksum:i56EE7E04D95BC5C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66194 Genomic DNA Translation: CAA46957.1
X90565 Genomic DNA Translation: CAA62172.1
Z75225 Genomic DNA Translation: CAA99637.1
BK006948 Genomic DNA Translation: DAA11082.1
PIRiS23052
RefSeqiNP_014962.3, NM_001183737.3

Genome annotation databases

EnsemblFungiiYOR317W; YOR317W; YOR317W
GeneIDi854495
KEGGisce:YOR317W

Similar proteinsi

Entry informationi

Entry nameiLCF1_YEAST
AccessioniPrimary (citable) accession number: P30624
Secondary accession number(s): D6W316
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 25, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome