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P30624

- LCF1_YEAST

UniProt

P30624 - LCF1_YEAST

Protein

Long-chain-fatty-acid--CoA ligase 1

Gene

FAA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It may supplement intracellular myristoyl-CoA pools from exogenous myristate. Preferentially acts on C12:0-C16:0 fatty acids with myristic and pentadecanic acid (C15:0) having the highest activities.

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

    Cofactori

    Magnesium.

    Temperature dependencei

    Optimum temperature is 30 degrees Celsius.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. long-chain fatty acid-CoA ligase activity Source: SGD
    3. medium-chain fatty acid-CoA ligase activity Source: SGD

    GO - Biological processi

    1. long-chain fatty acid metabolic process Source: GOC
    2. long-chain fatty acid transport Source: SGD
    3. long-chain fatty-acyl-CoA metabolic process Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YOR317W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long-chain-fatty-acid--CoA ligase 1 (EC:6.2.1.3)
    Alternative name(s):
    Fatty acid activator 1
    Long-chain acyl-CoA synthetase 1
    Gene namesi
    Name:FAA1
    Ordered Locus Names:YOR317W
    ORF Names:O6136
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR317w.
    SGDiS000005844. FAA1.

    Subcellular locationi

    GO - Cellular componenti

    1. lipid particle Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 700700Long-chain-fatty-acid--CoA ligase 1PRO_0000193119Add
    BLAST

    Proteomic databases

    MaxQBiP30624.
    PaxDbiP30624.
    PeptideAtlasiP30624.

    Expressioni

    Gene expression databases

    GenevestigatoriP30624.

    Interactioni

    Protein-protein interaction databases

    BioGridi34704. 68 interactions.
    DIPiDIP-4654N.
    IntActiP30624. 1 interaction.
    MINTiMINT-541922.
    STRINGi4932.YOR317W.

    Structurei

    3D structure databases

    ProteinModelPortaliP30624.
    SMRiP30624. Positions 112-604.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1022.
    GeneTreeiENSGT00690000102168.
    HOGENOMiHOG000159459.
    KOiK01897.
    OMAiTETCANT.
    OrthoDBiEOG7CCC0K.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30624-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAQYTVPVG KAANEHETAP RRNYQCREKP LVRPPNTKCS TVYEFVLECF    50
    QKNKNSNAMG WRDVKEIHEE SKSVMKKVDG KETSVEKKWM YYELSHYHYN 100
    SFDQLTDIMH EIGRGLVKIG LKPNDDDKLH LYAATSHKWM KMFLGAQSQG 150
    IPVVTAYDTL GEKGLIHSLV QTGSKAIFTD NSLLPSLIKP VQAAQDVKYI 200
    IHFDSISSED RRQSGKIYQS AHDAINRIKE VRPDIKTFSF DDILKLGKES 250
    CNEIDVHPPG KDDLCCIMYT SGSTGEPKGV VLKHSNVVAG VGGASLNVLK 300
    FVGNTDRVIC FLPLAHIFEL VFELLSFYWG ACIGYATVKT LTSSSVRNCQ 350
    GDLQEFKPTI MVGVAAVWET VRKGILNQID NLPFLTKKIF WTAYNTKLNM 400
    QRLHIPGGGA LGNLVFKKIR TATGGQLRYL LNGGSPISRD AQEFITNLIC 450
    PMLIGYGLTE TCASTTILDP ANFELGVAGD LTGCVTVKLV DVEELGYFAK 500
    NNQGEVWITG ANVTPEYYKN EEETSQALTS DGWFKTGDIG EWEANGHLKI 550
    IDRKKNLVKT MNGEYIALEK LESVYRSNEY VANICVYADQ SKTKPVGIIV 600
    PNHAPLTKLA KKLGIMEQKD SSINIENYLE DAKLIKAVYS DLLKTGKDQG 650
    LVGIELLAGI VFFDGEWTPQ NGFVTSAQKL KRKDILNAVK DKVDAVYSSS 700
    Length:700
    Mass (Da):77,866
    Last modified:April 1, 1993 - v1
    Checksum:i56EE7E04D95BC5C9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66194 Genomic DNA. Translation: CAA46957.1.
    X90565 Genomic DNA. Translation: CAA62172.1.
    Z75225 Genomic DNA. Translation: CAA99637.1.
    BK006948 Genomic DNA. Translation: DAA11082.1.
    PIRiS23052.
    RefSeqiNP_014962.3. NM_001183737.3.

    Genome annotation databases

    EnsemblFungiiYOR317W; YOR317W; YOR317W.
    GeneIDi854495.
    KEGGisce:YOR317W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66194 Genomic DNA. Translation: CAA46957.1 .
    X90565 Genomic DNA. Translation: CAA62172.1 .
    Z75225 Genomic DNA. Translation: CAA99637.1 .
    BK006948 Genomic DNA. Translation: DAA11082.1 .
    PIRi S23052.
    RefSeqi NP_014962.3. NM_001183737.3.

    3D structure databases

    ProteinModelPortali P30624.
    SMRi P30624. Positions 112-604.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34704. 68 interactions.
    DIPi DIP-4654N.
    IntActi P30624. 1 interaction.
    MINTi MINT-541922.
    STRINGi 4932.YOR317W.

    Proteomic databases

    MaxQBi P30624.
    PaxDbi P30624.
    PeptideAtlasi P30624.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR317W ; YOR317W ; YOR317W .
    GeneIDi 854495.
    KEGGi sce:YOR317W.

    Organism-specific databases

    CYGDi YOR317w.
    SGDi S000005844. FAA1.

    Phylogenomic databases

    eggNOGi COG1022.
    GeneTreei ENSGT00690000102168.
    HOGENOMi HOG000159459.
    KOi K01897.
    OMAi TETCANT.
    OrthoDBi EOG7CCC0K.

    Enzyme and pathway databases

    BioCyci YEAST:YOR317W-MONOMER.

    Miscellaneous databases

    NextBioi 976828.

    Gene expression databases

    Genevestigatori P30624.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a Saccharomyces cerevisiae long chain fatty acyl:CoA synthetase gene (FAA1) and assessment of its role in protein N-myristoylation."
      Duronio R.J., Knoll L.J., Gordon J.I.
      J. Cell Biol. 117:515-529(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 4-14.
      Strain: ATCC 204508 / S288c.
    2. "Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome XV reveals regions of similarity to chromosomes I and XIII."
      Pearson B.M., Hernando Y., Payne J., Wolf S.S., Kalogeropoulos A., Schweizer M.
      Yeast 12:1021-1031(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Biochemical studies of three Saccharomyces cerevisiae acyl-CoA synthetases, Faa1p, Faa2p, and Faa3p."
      Knoll L.J., Johnson D.R., Gordon J.I.
      J. Biol. Chem. 269:16348-16356(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLCF1_YEAST
    AccessioniPrimary (citable) accession number: P30624
    Secondary accession number(s): D6W316
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7470 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3